单克隆抗体的基因工程

本文介绍了抗体基因工程的最新进展，其中包括用聚合酶链反应（ＰＣＲ）从杂交瘤或非免疫细胞基因库快速克隆抗体可变区（Ｖ区）基因，互补决定区（ＣＤＲ）移植Ｖ区基因的设计和构建，Ｆｖ和单链Ｆｖ片段的构建等方法，以及载体扩增在哺乳动物细胞或大肠杆菌中高效表达抗体及其片段的技术等。     

大肠腺瘤—腺瘤癌变—腺癌序列中多基因异常表达的检测及意义

大肠腺瘤┐腺瘤癌变┐腺癌序列中多基因异常表达的检测及意义李正生郁宝铭王瑞年秦兰芳尹浩然林言箴关键词大肠腺瘤┐腺瘤癌变┐腺癌序列ｐ５３ＰＣＮＡＥＧＦＲ作者单位：上海第二医科大学附属瑞金医院普外科（上海２０００２５）Ｖｏｇｅｌｓｔｅｉｎ等［１］在实验的基...     

SURGICAL TREATMENT AND LATE RESULTS IN l,226 COLORECTAL CANCER CASES

1,226 cases of colorectal cancer were treated surgically between 1956-1978. 798 (65.09%) were rectal, 74.3% 0f which were located below the peritoneal reflection. Dukes C2 cases and cases with distant metastasis constituted 39.15To and Dukes' A cases 9.22%. Resectability rate was 75.1% (77.6% for rectal cancer). 32.96% of 619 cases of rectal excision had restorative resection. 84.8% 0f the combined excision for extraperi- toneal lesions in female patients were posterior pelvic exenteration. The overall operative mor- tality rate was 2.93%. Mortality rate of resec- tion was, 1.73(70 and that of rectal resection 0.8%。 and curative rectal resection 0.63%. Follow up rate was 94.13% The 5- and 10-year survival rates for rectal resection were 53.08To and 47.65%, for curative rectal resection 66.91To and 60.27To, and for Dukes' A cases 98.05% and 96.39%. The 5- and 10-year survival rates for colonic resec- tion were 59.79% and 52.18%, for curative colonic resection 72.79% and 62.06%, and for Dukes' A cases both 100%. Among factors affecting the- rapeutic results, local immunologic reaction of the host is also important, showing that the more lymphocytic infiltration in and around cancer, and the more follicular hyperplasia and sinus histiocytosis in regiona.l lymph nodes the better the prognosis. Anal preservation in radical re- section of rectal cancer and improving results in the treatment of extraperitoneal rectal cancer are discussed in detail.     

启东市未婚青年生殖健康知识问卷调查结果分析

<正>"2015年人人享有生殖保健"是1994国际人口与发展大会提出的一个全球性奋斗目标,1995年在第四届世界妇女大会上又一次强化了这一目标。生殖健康是人类健康的核心,生殖健康的新概念已经越来越深入地被人们所接受,大家越来越意识到生殖健康与提高生命质量密切相关,它是幸福生活的基本前提。为了解我市未婚青年对生殖健康知识的掌握程度和对生殖健康教育及服务的需求,我们对2003年7-9月在我所进行婚前体检的980例未婚男女进行了生殖健康知识问卷调查,现将结果报告如下。     

蛇鳖软膏灸治疗腰椎间盘突出症临床观察及机理探讨

将１０５例腰椎间盘突出症患者（突出物≤０３ｃｍ）,按２∶２∶１的比例随机分为３组,即蛇鳖软膏灸治疗组４２例,单用蛇鳖软膏为对照１组４２例,外贴硬膏麝香止痛膏为对照２组２１例。统计学分析结果显示,治疗组与对照１组相比,有效率差异无显著意义（Ｐ＞００５）,但在优良率上前者明显较高（Ｐ＜００５）;对照１组与对照２组比较,疗效差异有非常显著意义（Ｐ＜０００５）。     

针炙治疗腰椎间盘突出症的临床研究

将蛇鳖软膏灸配合牵引态下针刺下单纯牵引态下针刺治疗随机分组,进行疗效对比分析。结果：两组在统计学差异有显著意义,前者在多项指标上优于后者。：牵引态下针刺能发迹突出物与受刺激根之间的位置关系,蛇软膏灸能抑制神经根水肿和炎性反应。两者 事,能疗效、缩短疗程、减少后遗传症状。     

Relationship between angiotensinogen gene M235T variant with diabetic nephropathy in Chinese NIDDM

Ｏｂｊｅｃｔｉｖｅ　Ｔｏｉｎｖｅｓｔｉｇａｔｅｗｈｅｔｈｅｒａｎｇｉｏｔｅｎｓｉｎｏｇｅｎ（ＡＧＴ）ｇｅｎｅＭ２３５Ｔｖａｒｉａｎｔｉｓａｓｓｏｃｉａｔｅｄｗｉｔｈｎｏｎｉｎｓｕｌｉｎｄｅｐｅｎｄｅｎｔｄｉａｂｅｔｅｓｍｅｌｌｉｔｕｓｗｉｔｈｏｕｔｎｅｐｈｒｏｐａｔｈｙ（ＤＮ－）,ａｎｄｄｉａｂｅｔｉｃｎｅｐｈｒｏｐａｔｈｙ（ＤＮ＋）ｉｎＣｈｉｎｅｓｅｎｏｎｉｎｓｕｌｉｎｄｅｐｅｎｄｅｎｔｄｉａｂｅｔｅｓｍｅｌｌｉｔｕｓ（ＮＩＤＤＭ）Ｍｅｔｈｏｄｓ　ＴｈｅｓｕｂｊｅｃｔｓｉｎＤＮ＋ｇｒ…