益妇宁软胶囊对去势大鼠骨质疏松症的影响

目的:以去卵巢大鼠为实验对象,观察益妇宁软胶囊(YFN)对绝经后骨质疏松症的影响。方法:采用3月龄SD雌性大鼠,行双侧卵巢切除术,随机分为YFN大、中、小剂量组和阳性药对照组、模型对照组及假手术组。术后4周开始给药,给药12周后收集标本。采用放免法测定血清雌二醇水平,血清钙、磷、碱性磷酸酶含量;称量子宫重量;测量大鼠肱骨长度、横径及肱骨干重、湿重;采用原子吸收光谱法测定骨矿物质钙、镁、锌、锰含量;使用双能x线骨密度仪测定离体股骨密度。结果:VFN能明显提高去势大鼠体内雌激素水平,增加性器官重量;使去势大鼠骨长、宽、干重、湿重增加;增加骨钙、镁、锌、锰等矿物质含量;增强绝经后疏松骨质的密度。结论:YFN能起到治疗绝经后骨质疏松症的作用。     

益妇宁软胶囊对去势大鼠β-EP的影响

目的 观察益妇宁软胶囊对去势大鼠血清性激素及下丘脑、全体和血浆中β—内啡肤(β—EP)水平的影响。方法 给药4周后用放射免疫法测定各实验组大鼠血清性激素及下丘脑、全体和血浆中β—EP水平，并测定大鼠体重及子宫指数。结果 益妇宁能明显升高大鼠去势后降低的血清E2水平和子宫指数(P＜0．05或P＜0、01)；提高去势大鼠下丘脑、全体和血浆中β—EP水平(P＜0.01或P＜0．05)。结论 益妇宁软胶囊通过调节生殖内分泌功能和β—EP神经递质功能，从而起到稳定机体内环境和缓解围绝经期综合征的作用。     

黄芫花乙醇液抗早孕312例临床分析

目的 :了解自制黄芫花乙醇液抗早孕 (孕周小于 12周 )临床效果。方法 :回顾性调查了本院 1979～1998年 3 12份临床病历并进行了分析。结果 :显示其完全流产率为 83 .97% ,总有效率为 94 .5 5 % ,与前列腺素类似物抗早孕效果相似。流产时间的中位数为 2 0h 11min ;平均流血量为 ( 78.18± 77.2 5 )ml。在完全流产的病历中 ,初孕、经孕未产与经产妇 3组间的流产率无显著性差异 ;流产率与年龄有非常显著差异 ,而与孕期无关。结论 :黄芫花乙醇液抗早孕是安全有效的。     

微米中药功能衬材：活血及促进骨折愈合的作用

BACKGROUND: As a new self-synthetized medical orthopedic fixation material, micron traditional Chinese medicine functional lining material can promote the secretion of alkaline phosphatase and callus formation, decrease serum calcium level, as well as increase serum phosphorus level and calcium-phosphorus product. OBJECTIVE: To further verify the therapeutic effect of micron traditional Chinese medicine functional lining material on fracture healing. METHODS: Twenty-four New Zealand rabbits were selected to prepare models of right radial fracture, and then were randomly allocated into four groups: rabbits in micron group were treated with micron traditional Chinese medicine functional lining material (micron Panax notoginseng powder mixed with active ingredients of Davallia divaricata and Carthamus tinctoriousplus) plus plaster external fixation; those in traditional Chinese medicine control group treated with ordinary Chinese medicine functional material plus plaster external fixation; those in lining material control group treated with ordinary lining material (sterile degreasing cotton) plus plaster external fixation; those in model control group received no treatment. The whole blood viscosity at high/middle/low shear rates and plasma viscosity were detected at 2, 4 and 6 weeks before and after modeling. Additionally, rabbits in each group were killed at 6 weeks after modeling, and radial samples were removed for histological observation. RESULTS AND CONCLUSION: The whole blood viscosity at high/middle/low shear rates and plasma viscosity in the micron group were all lower than those in the other three groups in the same period, and had significant differences from those in the model control group at 4 and 6 weeks after modeling (P < 0.05 or 0.01). A large number of osteoblasts could be found, woven bone began to form and the bone marrow cavity was recanalized in the micron group; in the traditional Chinese medicine group, woven bone, chondrocytes and endochondral ossification coexisted and there was a connection between calluses inside and outside of the broken end, but the bone marrow cavity was not recanalized. In contrast, neither connection between calluses inside and outside of the broken end, nor recanalized bone marrow cavity appeared in the lining material and model control groups. In conclusion, micron traditional Chinese medicine functional lining material can promote fracture healing by improving microcirculation and accelerating osteoblast proliferation.     

肠益康汤对脾虚泄泻小鼠肠道功能的保护作用

目的观察肠益康汤对脾虚动物肠道菌群失调及胃肠功能异常的调整作用。方法用大黄诱导脾虚泄泻小鼠模型,连续灌胃给予肠益康汤14d,采用镜检法测定肠道菌群水平,墨汁印迹法检测小肠推进率,电镜观察小肠黏膜。结果与模型组比较,肠益康汤高、中、低剂量组小鼠双歧杆菌和乳酸杆菌数量显著增加(P0.05),肠益康高、中剂量组小鼠小肠碳末推进率显著降低(P0.05),肠益康汤高、中、低剂量组小鼠小肠黏膜病理改变显著改善。结论肠益康汤能调节肠道菌群紊乱,抑制脾虚小鼠肠推进功能亢进,并对大黄致脾虚小鼠小肠黏膜的病理改变也有一定的保护作用。     

肾衰宁灌肠液对腺嘌呤所致慢性肾衰模型大鼠的影响

肾衰宁灌肠液灌肠给药对含0．5％腺嘌呤饲料所致大鼠慢性肾功能衰竭模型有显的保护作用,可降低血清肌酐、尿素氮含量,对提高红细胞及血红蛋白含量也有一定的效果。     

紫外分光光度法测定复方红景天片中总黄酮含量

目的:建立以紫外分光光度法测定复方红景天片中总黄酮含量的方法。方法:采用紫外分光光度法,检测波长为254nm,以大豆苷元为标准品测定本品总黄酮含量。结果:本品中大豆苷元浓度在0.8～6.4mg·L^-1范围内与吸光度线性关系良好(r=0.9999),平均回收率为100.76%(RSD=0.37%)。结论:该方法准确可靠,操作简便,稳定性好。     

哈蟆油对D-半乳糖所致雄性衰老大鼠肝组织CyclinD1、CDK6和P15蛋白表达的影响

目的 探讨哈蟆油对D-半乳糖所致雄性衰老大鼠肝组织细胞周期素D1(CyclinD1)、周期性依赖性激酶6(CDK6)和细胞周期蛋白D/CDK激酶的抑制因子P15蛋白表达调控的影响.方法 SPF级SD雄性大鼠18只,随机分为模型、维生素E(VE)和哈蟆油组,每组6只,D-半乳糖颈背部皮下连续注射6周,另取雄性大鼠6只,同样部位每日注射生理盐水,作为空白组.第3周开始灌胃给药,给药时间30 d.给药结束后收集标本采用Western blotting法检测肝组织CyclinD1、CDK6和P15蛋白的表达.结果 与空白组比较,模型组大鼠肝脏CyclinD1蛋白、CDK6蛋白、P15蛋白表达明显下调.雄性衰老大鼠口服哈蟆油后,CyclinD1、CDK6和P15蛋白表达量(与内参比较)分别为41.73±0.54、23.29±0.30和21.49±0.30.与模型组相比,哈蟆油组能明显上调肝脏Cyelin D1、CDK6、P15蛋白的表达(P＜0.01).结论 哈蟆油能不同程度上调CyclinD1和CDK6蛋白表达,促进衰老雄性大鼠肝细胞增殖.同时反馈性上调细胞增殖负性调控蛋白P15表达,防止不必要的过度增殖.哈蟆油延缓雄性衰老作用可能通过调控肝脏有关增殖蛋白表达发挥抗衰老作用,与VE作用有所不同.     

槐定碱、苦参碱和苦豆碱对LPS诱发结肠上皮细胞炎症模型中细胞因子IL-6和TNF-α水平的影响

目的：构建结肠上皮细胞炎症模型,探讨槐定碱、苦参碱和苦豆碱对细胞因子白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）表达的影响。方法：将细胞接种12孔板中,每孔接种肠上皮细胞株IEC-6 2×10⁵个细胞。细菌脂多糖（LPS,1 mg·L^-1）干预生长良好的IEC-6细胞12 h,建立细胞炎症模型。四甲基偶氮唑盐（MTT）法检测3种生物碱不同浓度对炎症细胞增殖的影响;ELISA法检测给药后细胞上清中IL-6,TNF-α的变化;免疫印迹法检测细胞内IL-6及TNF-α蛋白表达水平。结果：与正常组比较,模型组细胞上清中IL-6,TNF-α水平显著升高,LPS （1 mg·L^-1）干预12 h后,IL-6,TNF-α含量分别为（1 899.68±45.80）,（148.67±5.93） ng·L^-1。苦豆碱、槐定碱和苦参碱均可有效减弱LPS诱导的结肠上皮细胞损伤。当生物碱浓度为100 μmol·L^-1时,槐定碱、苦参碱和苦豆碱均可提高LPS损伤细胞的活性,细胞存活率分别从80.47%提高为91.96%,92.40%,97.23%。此外,这3种生物碱可降低IL-6和TNF-α的含量及其在细胞内的表达。结论：槐定碱、苦参碱和苦豆碱3种生物碱均可通过降低IL-6和TNF-α活性发挥抗炎作用。其中,苦豆碱的抗炎作用最佳。     

天然可降解多糖材料在口服结肠定位释药制剂中的应用

结肠定位释放制剂是近年来药剂学研究的热点之一。论述了基于菌触发型结肠定位策略的优点,分析了肠道菌群、酶在消化道的分布、种类、量的变化以及不同菌属对多糖降解的差异。天然多糖来源广泛,毒性极低,性质稳定且多数有一定的免疫调节、抗炎、抗肿瘤等功效,是酶、多肽、激素等药物的良好载体。综述直链淀粉、果胶、瓜儿胶、海藻酸类、壳聚糖在口服结肠定位释放制剂中的国外最新应用进展,为更好地利用多糖材料开发结肠定位递送制剂提供文献支持。