孤啡肽对大鼠离体心脏功能的调节作用及其对去甲肾上腺素诱发心脏功能变化的影响

Objective To investigate the effects of orphanin FQ (OFQ) on simple and norepinephrineactivated cardiac function in isolated rat heart.Methods Male SD rats aged 3-4 months weighing 250-280 g were anesthetized with intraperitoneal 25% urethane 1.4-1.8 ml.Their hearts were excised and passively perfused with K-H solutionin a Lanngendorff apparatus.A fluid-filled latex balloon was inserted into left ventricle for measurement of developed pressure.Left ventricular systolic pressure(LVSP),left ventricular end-diastofic pressure(LVEDP),left ventricular developed pressure(LVDP=LVSP-LVEDP),HR and±dp/dtmax were measured.Sixty isolated rat hearts were randomly divided into 10 groups(n=6 each):Ⅰ control group (group C);Ⅱ,Ⅲ,Ⅳ OFQ1,OFQ2,OFQ3 group--OFQ 10-6,10-7,10-8 mol/L were administered via perfusion system respectively;Ⅴ OFQ+UFP group--OFQ 10-6 mol/L+UFP-101 (all OFQ antagonist) 10-6 mol/L were administered;Ⅵ NE group--norepinephrine 10-5 mol/L;Ⅶ,Ⅷ,Ⅸ NE+OFQ groups--NE 10-5 mol/L+OFQ 10-6,10-7,10-8 mol/L were administered respectively;Ⅹ NE+OFQ+UFP group--NE 10-5 mol/L+OFQ 10-6mol/L+UFP-101 10-6mol/L.Results LVSP,LVDP and±dp/dtmax were significantly increased while HR was significantly decreased in the 3 OFQ groups as compared with control group.LVSP,LVDP±dp/dt max and HR were significantly increased in the 3 NE+OFQ groups as compared with NE group.Conclusion OFQ has positive inotropic action and negative chronotropic effect on the isolated rat heart.OFQ Can enhance the positive inotropic and chronotropic effect of NE on the heart.     

复方甘草酸苷制剂对脂多糖诱导小鼠RAW264.7细胞分泌炎症因子的调节作用

目的:研究复方甘草酸苷片剂和注射剂对脂多糖诱导小鼠巨噬细胞RAW 264.7分泌炎症因子的调节作用。方法:采用脂多糖诱导的小鼠巨噬细胞RWA264.7,建立体外炎症模型。取复方甘草酸苷片剂和注射剂,制备供试药液。分别通过,MTT、Griess和双抗体夹心ABC-ELISA等实验,测定在不同浓度的供试药液作用下RAW264.7细胞活力以及经脂多糖诱导后的RAW264.7细胞的一氧化氮、肿瘤坏死因子TNF-α及白介素IL-1β、-6和-10等炎症因子分泌量的变化。结果:复方甘草酸苷的2种制剂药液在0～300μmol.L-1浓度范围内对RAW264.7细胞活力无影响。复方甘草酸苷注射剂药液在75、150和300μmol.L-1浓度下对经脂多糖处理的RAW264.7细胞的一氧化氮分泌抑制率分别为13.8%、40.4%和53.8%,并致细胞的TNF-α分泌量降低29.8%、41.5%和52.1%,IL-1β分泌量降低39.5%、55.6%和69.6%,IL-6分泌量降低18.3%、29.5%和39.1%,但IL-10分泌量却提高了8.2%、23.8%和30.8%;而复方甘草酸苷片药液在相同浓度下对同样细胞的一氧化氮分泌抑制率分别为9.8%、27.1%和37.8%,致细胞的TNF-α分泌量降低16.6%、37.0%和48.4%,IL-1β分泌量降低28.1%、47.9%和57.9%,IL-6分泌量降低13.5%、19.9%和28.2%,IL-10分泌量提高了3.9%、14.8%和23.4%。复方甘草酸苷的2种制剂对细胞分泌炎症因子的调节作用与阳性对照药地塞米松相似,其中注射剂的作用强于片剂。结论:复方甘草酸苷可剂量依赖性地显著抑制脂多糖诱导小鼠RAW 264.7细胞产生促炎因子一氧化氮、TNF-α及IL-1β和-6并促进抗炎因子IL-10的表达,这可能为其抗炎作用机制。     

两种宫内节育器临床效果比较

目的观察MCuⅡ功能性宫内节育器(MCuⅡ intrauterine contraceptivedevice,MCuⅡIUD)的妊娠率、脱落率、因症取出率和副反应发生率.方法采用多中心随机对照,将MCuⅡIUD与TCu220C宫内节育器(TCu220CIUD)进行临床比较.常规放置IUD,放置后第1、3、6、12个月进行随访观察.结果放置MCuⅡIUD与TCu220CIUD各500例,置器满12个月累积带器妊娠率依次为0.20%、0.41%;脱落/下移取出率分别为0.20%、1.62%,差异有统计学意义(P<0.05);因症取出率分别为2.21%、2.65%;置器后4次随访主诉(副反应)两组间差异均有统计学意义(P<0.05).结论 MCuⅡIUD带器妊娠率低、脱落率低、因症取出率低和副反应发生率低的IUD,值得临床推广.     

外科护理记录的书写应注意的问题与解决对策

<正>护理记录是医院具有法律效力的重要文件之一,必须要全面、真实、准确、及时的完成,它不仅反映了护理质量的高低,也是对护士自身的一种保护。外科临床护士在书写护理记录时涉及许多潜在的法律问题,对护理病历的书写提出了     

P物质对去甲肾上腺素诱发大鼠离体心脏室性心律失常的影响

P物质(SP)是一种神经肽,广泛分布于中枢和外周神经系统中,具有多种生物学作用.前期研究表明,大鼠急性心肌缺血时心肌组织内SP水平增加[1,2],与血液中儿茶酚胺浓度急剧升高的时间相重叠[3],可能存在SP与儿茶酚胺的相互作用.急性心肌缺血时交感神经兴奋,去甲肾上腺素(NE)过度释放,可导致心律失常等.本研究拟评价SP对去甲肾上腺素诱发大鼠离体心脏室性心律失常的影响.     

谈大学英语课堂内外的文化导入

传统英语教学过于侧重讲解语言基础知识,但是大学英语课程不仅是一门语言基础课程,更是使学生了解世界文化,增强跨文化交际能力的素质课程,因此应该重视文化因素在教学中的意义。本文列举了在教学中存在的与文化有关的问题,提出应在课堂内外进行文化导入,以期能够激发学生的学习积极性,提高学生的跨文化交际能力。     

解析护理工作中的护患沟通

护理工作的服务对象是众多不同年龄、性别、职业、社会地位、文化教养的人。沟通交流是护理工作中一项职业技术。护士通过与患者进行沟通,获取宝贵的一手资料,进行有的放矢的护理,对于促进患者的康复,提高患者的满意度,提高护理质量,意义重大。在交流沟通中,倾听与反馈尤为重要。     

多囊卵巢综合征患者血清内脂素和高敏C反应蛋白水平的测定与临床意义

Objective To explore serum levels of visfatin and high-sensitivity C-reactive protein (Hs-CRP) in patients with polycystic ovary syndrome (PCOS), as well as their clinical significance. Methods Ninety patients with PCOS and 30 ovulating healthy women with body mass index (BMI) less than 25 as controls were recruited. PCOS patients were divided to two groups, 48 in obese group with BMI≥ 25 and 42 in non-obese group with BMI<25. Serum level of visfatin was measured by enzyme-linked immunosorbent assay (ELISA) and that of Hs-CRP was assayed by immunoturbidimetry in all participants. Oral glucose tolerance test (OGTT) and insulin sensitivity were assessed in PCOS patients only. Results ①Obese and non-obese patients with PCOS beth presented markedly higher serum levels of visfatin and Hs-CRP than those in controls (t=11.35, 8.46, 10.43 and 8.25, respectively, P<0.05), and levels of visfatin and Hs-CRP were higher in obese group than those in non-obese group (t=7.74 and 6.38, respectively, P< 0.05 ). ②Univariate analysis revealed that serum levels of visfatin and Hs-CRP positively correlated with BMI and homeostasis model assessment insulin resistance, respectively (P<0.05) and serum level of visfatin positively correlated with that of Hs-CRP (P<0.05). Conclusions Chronic inflammation possibly exists in patients with PCOS, which may cause their serum visfatin and Hs-CRP levels increased.     

5例关节置换术后低血压原因分析及护理对策

<正>膝、髋关节置换术为骨科常见手术,常用麻醉方法为硬膜外麻醉,术前需要常规禁食12 h、禁水8 h,术后6 h才可进流质饮食;关节置换术大多为老年患者,老年患者各种器官代偿功能减退,分解、清除、中和药物毒性的能力下降,失血相对较多,血流动力学不稳,容易导致心血管意外[1];该手术髓腔需注入骨水泥,机体条件差可能会造成低血压[2]。术后伤口     

急性重度一氧化碳中毒三种不同氧疗方法的疗效比较

目的对急性重度一氧化碳中毒患者,采用临床氧疗的方法,并对三种不同的氧疗方法的疗效进行探讨和比较。方法选取本县医院在2009年至2012年收治的90例患者,经过病例检测均为急性重度一氧化碳中毒,并对不同氧疗的治疗方法的治疗效果进行探讨和分析。结果采用鼻导管吸氧治疗组患者共30例,治愈率为57%,治愈人数为17例,并发症为7例;采用无创机械通气治疗组患者30例,治愈率67%,治愈人数为20例,并发症为5例;采用高压氧治疗组患者30例,治愈率为96%,治愈人数为29例,并发症患者1例。结论经过上述方法,可得出,在三种治疗方法中,高压氧治疗方法对患者的治疗具有显著疗效。但目前,在临床急救治疗上,适合采用具有方便、快捷、安全的无创机械通气方法,且该方法值得在临床急救治疗上推广。