麝香乌龙丸对佐剂性关节炎大鼠激素水平的影响

目的:研究麝香乌龙丸对佐剂性关节炎大鼠激素水平调节的影响,探讨麝香乌龙丸对类风湿性关节炎(RA)作用机制是否与调节激素水平有关.方法:建立佐剂性关节炎大鼠模型,麝香乌龙丸灌胃1周后,测定血清皮质醇(Cor)、血浆促肾上腺皮质激素(ACTH)及肾上腺维生素C含量的变化.结果:麝香乌龙丸能升高佐剂性关节炎(AA)大鼠Cot含量,降低肾上腺维生素C含量,与模型组比较有统计学意义(P＜0.05);能降低ACTH水平,但与模型组比较无统计学意义.结论:麝香乌龙丸作用与刺激.肾上腺增强其激素分泌功能有关.     

c-kit基因突变对胃肠道间质瘤预后影响的荟萃分析

目的 探讨c-kit基因突变对胃肠道间质瘤(GIST)预后的影响.方法 通过电子检索PubMed、MedLine数据库,对1999年1月至2008年8月有关c-kit基因突变对GIST预后影响相关病例对照研究资料,用RevMan 5.0.15软件进行荟萃分析.结果 共纳入文献16篇,共计患者1751例.其中与细胞病理学相关统计显示c-kit基因野生型与突变型在有丝分裂计数>5/50 HPF(高倍视野)的病例发生率上差异无统计学意义(P=0.710);与术后复发转移发生率相关统计显示,c-kit基因突变型术后复发转移的发生率显著高于野生型者(P=0.010);与伊马替尼疗效相关统计显示,c-kit基因突变型患者伊马替尼治疗缓解率显著高于野生型(P=0.009),而伊马替尼耐药率较低(P=0.000).亚组分析发现,外显子11突变型的患者伊马替尼治疗缓解率显著高于野生型和外显子9突变型者(P均<0.05),而伊马替尼耐药率较低(P均<0.05).c-kit基因二次突变与伊马替尼获得性耐药相关研究发现,二次突变区域集中在外显子13、14和17,部分为混合型突变.结论 c-kit基因突变与GIST术后复发转移的发生有着密切关系,c-kit基因突变可能会影响伊马替尼的疗效,而二次突变的发生可能是GIST产生耐药的主要原因之一.     

麝香乌龙丸对佐剂性关节炎大鼠性激素水平影响的研究

目的:研究麝香乌龙丸对佐剂性关节炎大鼠性激素水平调节的影响,探讨麝香乌龙丸治疗类风湿关节炎是否与调节性激素水平有关。方法:建立佐剂性关节炎大鼠模型,麝香乌龙丸灌胃1w后,测定血清中睾酮(T)、雌二醇(E2)及孕酮(P)的水平。结果:麝香乌龙丸能上调雄性大鼠血清T、E2水平以及雌性大鼠E2、P水平,与模型组比较有统计学意义(P<0.05)。结论:麝香乌龙丸治疗类风湿关节炎机理与调节性激素水平有关。     

CO2气腹环境对胃癌细胞黏附与侵袭转移的影响

Objective To investigate the influence of CO2 pneumoperitoneum on the adhesive and invasive ability of gastric cancer cells based on the expression of adhesive and invasive molecules. Methods With an artificial CO2 pneumoperitoneum model in vitro, human gastric cancer cells MKN-45, SGC-7901 and MKN-28 were exposed to 3 different CO2 gradients: 9 mm Hg, 15 mm Hg and control group (0 mm Hg). The expression of E-cadherin, ICAM-1, MMP-2 and VEGF-A were measured at 2 and 4 hours exposure by using RT-PCR, CytoMatrixTM kit and ECMatrixTM kit. The pretreated gastric cancer cells were injected into abdominal cavity of nude mice(2×106 cells per mouse). Five mice in each group were sacrificed 4 weeks later to record the number of tumor nodules in abdominal cavity. The remaining mice were kept for observation of survival time. Results The expression of E-cadherin (MKN-45: from 2.26 to 2.19, SGC-7901 :from 2.16 to 2.09、MKN-28 :from 2.06 to 1.99), ICAM-1 (MKN-45 : from 2.20 to 2.28、SGC-7901: from 2.10 to 2.18、MKN-28: from 2.00 to 2.08), MMP-2 (MKN-45:from 2.05 to 2.13、SGC-7901: from 1.95 to 2.03、MKN-28: from 1.85 to 1.93) and VEGF-A(MKN-45 : from 2.10 to 2.16、SGC-7901 :from 2.00 to 2.06、MKN-28: from 1.90 to 1.96) didn't change significantly with increasing pressure and time (P>0.05). The expression of adhesive and invasive molecules didn't change significantly between the experimental groups and the control group. There was no statistical significance of tumor metastasis in abdominal cavity of nude mice(MKN-45:from 22 to 23、SGC-7901 :from 20 to 22、MKN-28:from 21 to 22) and survival time(MKN-45 :from 23 to 21、SGC-7901 :from 22 to 21、MKN-28 :from 22 to 21) among all the groups. Conclusion Under low pressure and short time of CO2 exposure, the adhesive and invasive capacity of gastric cancer cells did not change significantly hence did not increase the possibility of neoplasm metastasis.     

CO2气腹环境对胃癌细胞黏附与侵袭转移的影响

Objective To investigate the influence of CO2 pneumoperitoneum on the adhesive and invasive ability of gastric cancer cells based on the expression of adhesive and invasive molecules. Methods With an artificial CO2 pneumoperitoneum model in vitro, human gastric cancer cells MKN-45, SGC-7901 and MKN-28 were exposed to 3 different CO2 gradients: 9 mm Hg, 15 mm Hg and control group (0 mm Hg). The expression of E-cadherin, ICAM-1, MMP-2 and VEGF-A were measured at 2 and 4 hours exposure by using RT-PCR, CytoMatrixTM kit and ECMatrixTM kit. The pretreated gastric cancer cells were injected into abdominal cavity of nude mice(2×106 cells per mouse). Five mice in each group were sacrificed 4 weeks later to record the number of tumor nodules in abdominal cavity. The remaining mice were kept for observation of survival time. Results The expression of E-cadherin (MKN-45: from 2.26 to 2.19, SGC-7901 :from 2.16 to 2.09、MKN-28 :from 2.06 to 1.99), ICAM-1 (MKN-45 : from 2.20 to 2.28、SGC-7901: from 2.10 to 2.18、MKN-28: from 2.00 to 2.08), MMP-2 (MKN-45:from 2.05 to 2.13、SGC-7901: from 1.95 to 2.03、MKN-28: from 1.85 to 1.93) and VEGF-A(MKN-45 : from 2.10 to 2.16、SGC-7901 :from 2.00 to 2.06、MKN-28: from 1.90 to 1.96) didn't change significantly with increasing pressure and time (P>0.05). The expression of adhesive and invasive molecules didn't change significantly between the experimental groups and the control group. There was no statistical significance of tumor metastasis in abdominal cavity of nude mice(MKN-45:from 22 to 23、SGC-7901 :from 20 to 22、MKN-28:from 21 to 22) and survival time(MKN-45 :from 23 to 21、SGC-7901 :from 22 to 21、MKN-28 :from 22 to 21) among all the groups. Conclusion Under low pressure and short time of CO2 exposure, the adhesive and invasive capacity of gastric cancer cells did not change significantly hence did not increase the possibility of neoplasm metastasis.     

左振素论治结节性红斑临床经验

总结名老中医左振素论治结节性红斑的临床经验。名老中医左振素崇尚"湿痰瘀"致病学术思想,以湿热痰瘀立论辨治结节性红斑,根据清热利湿祛痰、化瘀散结的治法确立治病基本方,临证注重分期论治,用药各有特色。急性期侧重湿热用药,稳定期侧重痰瘀用药,恢复期侧重祛除痰湿之源,调护脾胃,同时善于配合外治足浴之法调治,临床验案取得较好实效。     

泽泻汤加味治疗美尼尔氏综合征

美尼尔氏综合征在临床是常见病,属中医眩晕范畴,采用泽泻汤加味治疗42例,取得满意疗效,现报告如下。1临床资料1.1一般资料患者均系2000～2004年间我院门诊就诊的42例患者。男10例,女32例,年龄18～52岁,平均年龄30.5岁,眩晕每年发作1～3次12例,3～6次16例,7～12次10例,每月发作3～5次4例。病程在1年以内者16例,2年以内者22例,3年以上者4例。1.2诊断标准必备条件:①眩晕呈阵发性、突发性。②眩晕发作时伴耳鸣、耳闭及听力减退。③听力检查呈一侧感音性耳聋或混合性耳聋,属耳蜗性损害。④前庭功能检查为前庭终末期病变。次要条件:①眩晕呈旋转…     

麝香乌龙丸抗炎及镇痛药理研究

目的通过对麝香乌龙丸抗炎、镇痛的药理研究,为临床应用提供实验依据。方法建立小鼠耳廓肿胀、大鼠足跖肿胀、佐剂性关节炎和棉球肉芽肿炎症模型,研究其抗炎作用;采用小鼠扭体法和热板法,观察其镇痛作用。结果对小鼠耳廓炎症、大鼠足跖肿胀、佐剂性关节炎、棉球肉芽肿等均有明显抑制作用(P<0.01);能明显抑制炎性渗出液和白细胞趋化,并能明显减少冰醋酸引起的小鼠扭体次数、延长扭体出现时间和显著提高小鼠热板痛阈值(P<0.01)。结论麝香乌龙丸有明显的抗炎、镇痛作用。     

清宫长春胶囊治疗中医肾阳虚证疗效观察

目的：观察清宫长春胶囊对中医肾阳虚证的疗效。方法：采用随机、开放、阳性药平行对照的方法。试验组口服清宫长春胶囊，对照组口服金匮肾气丸，4周为1个疗程。在治疗前中后记录证候评分情况，治疗前后做血、尿、大便常规，肝肾功能及心电图检查。结果：治疗后，患者，临床症状得到改善，清宫长春胶囊和金匮肾气丸的总有效率分别为93．9％和86．4％，两组比较无统计学意义（P〉0．05）。结论：清宫长春胶囊和金匮肾气丸是治疗中医肾阳虚证安全、有效的药物。