经皮腰椎间盘后外侧入路"三角工作区"的薄层断面解剖和三维重建

目的 探明经皮腰椎间盘后外侧入路"三角工作区"的连续薄层断面的形态和三维结构.寻求解决"工作三角"的确切定义. 方法 选取2例中国数字化可视人体数据,对经皮腰椎问盘后外侧入路的连续断面形态进行解剖学观测并进行三维重建. 结果 获得经皮腰椎间盘后外侧入路的连续薄层断面形态学特征、相关测量参数和三维结构."三角工作区"的面积为(23.70±8.73)mm2,腰椎间盘穿刺虽是比较安全的区域,但有腰动脉脊支、椎间静脉和椎内静脉通过. 结论 经皮腰椎问盘后外侧入路"工作三角"的连续薄层断面数据集和三维重建可良好显示其形态和三维结构.初步明确了"工作三角"的定义,进一步证实腰动脉脊支、椎间静脉和椎内静脉是穿刺出血的解剖学基础.     

石骨症1例

1 病例资料 患者,女,38岁,摔伤致左大腿疼痛,肿胀,活动受限3月余.曾在外院行左下肢骨牵引50余天,未见明显骨痂生长,转入我科行切开复位钢板螺钉内固定.     

淋病奈瑟菌孔蛋白PIA真核表达系统的构建及序列分析

目的:构建淋病奈瑟菌孔蛋白PIA(Porin IA)基因的真核表达系统,为淋病DNA疫苗的研究提供材料。方法:PCR技术扩增淋病奈瑟菌捷克标准株的PIA基因片段,将其定向插入真核表达载体pC I-neo多克隆位点中,构建重组表达载体。并用酶切分析、PCR扩增及序列测定方法,对重组载体进行鉴定。结果:淋病奈瑟菌PIA基因被正确克隆到真核表达载体pC I-neo中,测序结果与Genebank中AF090819等菌株序列有99.99%同源性。结论:真核表达系统(pC I-PIA)构建成功。     

月经期断指再植围手术期的心理护理

通过对2008年6月～2012年3月本院收治的35例(42指)月经期手指离断性损伤行急诊清创断指再植术的系统护理资料进行回顾性分析,认为充分的术前评估、心理护理及术前准备,术中患者密切配合,术后严密的病情观察、积极对症处理及精心护理,是确保月经期手指离断性损伤患者手术成功的重要环节.     

基于网络药理学的补中益气丸抗疲劳机制研究

目的：探讨补中益气丸发挥抗疲劳作用的主要活性成分和潜在的机制。方法：通过中药系统药理学数据库与分析平台（TCMSP）筛选补中益气丸中的活性成分,利用Swiss Target Prediction、Drugbank、Cytoscape 3.7.1、R语言等数据库及软件预测活性成分作用靶点和“疲劳”相关靶点、构建疾病-药物-有效成分-靶基因的互作网络和蛋白质-蛋白质相互作用（PPI）网络,并进行关键靶基因的基因本体（GO）富集分析和京都基因和基因组百科全书（KEGG）富集分析,最后通过设计动物实验探究补中益气丸的抗疲劳药效。结果：分析表明与补中益气丸抗疲劳相关的163个潜在活性成分作用于与疲劳相关的108个靶点和160条通路。进一步网络特征分析发现表现主要活性成分为黄酮类和甾醇类化合物,通过参与脂多糖应答、对原细菌分子的反应等生物过程,调控MAPK、PI3K-AKT、TNF等信号通路进而发挥抗疲劳作用。药效学试验结果提示补中益气丸可显著增强小鼠运动耐力,但无显著缓解体力疲劳的效果。结论：补中益气丸具有多成分-多靶点-多途径的抗疲劳特点,其活性成分作用于多个靶点蛋白,调控多个生物过程及信号通路,起到改善机体氧化应激反应,调控细胞功能等作用,但在调节机体能量代谢方面未有明显的富集效果及药效学试验结果。本研究为进一步开发抗疲劳功能产品及药品和临床应用提供理论指导,为探究补中益气丸抗疲劳作用机制提供新思路。     

中药材定向培育分析评估系统的设计与实现

目的:建立科学的中药材定向培育分析评估系统。方法:基于地理信息技术的组件式开发方式,以.Net Framework为开发框架搭建C/S架构。结果:以生态环境因子库为基础,构建了一套完整的定向培育分析评估方案并建立了中药材定向培育分析评估系统。结论:构建的中药材定向培育分析评估系统能够解析中药材关键栽培技术与生长环境对中药材产量和质量的影响、指导中药材的施肥与灌溉等栽培技术、提高中药材定向培育种植过程中的科学性。     

Mitofusin2 decreases intracellular cholesterol of oxidized LDL-induced foam cells from rat vascular smooth muscle cells

Mitofusin2 (Mfn2) plays a pivotal role in the proliferation and apoptosis of vascular smooth muscle cells (VSMCs). The purpose of this study was to investigate the effects of Mfn2 on the trafficking of intracellular cholesterol in the foam cells derived from rat VSMCs (rVSMCs) and also to investigate the effects of Mfn2 on the expression of adenosine triphosphate-binding cassette subfamily A member 1 (ABCA1), adenosine triphosphate-binding cassette subfamily G member 1 (ABCG1) and peroxisome proliferator-activated receptor gamma (PPARγ). The rVSMCs were co-cultured with oxidized low density lipoprotein (LDL, 80 μg/mL) to produce foam cells and cholesterol accumulation in cells. Before oxidized LDL treatment, different titers (20, 40 and 60 pfu/cell) of recombinant adenovirus containing Mfn2 gene (Adv-Mfn2) were added into the culture medium for 24 h to transfect the Mfn2 gene into the rVSMCs. Then the cells were harvested for analyses. The protein expression of Mfn2 was significantly higher in Adv-Mfn2-transfected group than in untransfected group (P<0.05), and the expression levels significantly increased when the titer of Adv-Mfn2 increased (P<0.05). At 24 or 48 h after oxidized LDL treatment, rVSMCs became irregular and their nuclei became larger, and their plasma abounded with red lipid droplets. However, the number of red lipid droplets was significantly decreased in Adv-Mfn2-transfected group as compared with untransfected group. At 48 h after oxidized LDL treatment, the intracellular cholesterol in rVSMCs was significantly increased (P<0.05), but it was significantly decreased in Adv-Mfn2-transfected group as compared with untransfected group (P<0.05), and it also significantly decreased when the titer of Adv-Mfn2 increased (P<0.05). The mRNA and protein expression levels of ABCA1 and ABCG1 were significantly increased in Adv-Mfn2-transfected group as compared with untransfected group (P<0.05). Though the mRNA and protein expression levels of PPARγ was not significantly increased (P>0.05), the phosporylation levels of PPARγ were significantly decreased in Adv-Mfn2-transfected group as compared with untransfected group (P<0.05). These results suggest that the transfection of Adv-Mfn2 can significantly reduce intracellular cholesterol in oxidized LDL-induced rVSMCs possibly by decreasing PPARγ phosporylation and then increasing protein expression levels of ABCA1 and ABCG1, which may be helpful to suppress the formation of foam cells.     

加强总住院医师培养造就高质量临床医师

阐述了某医院培养总住院医师的做法和体会,认为总住院医师培养是医学人才培养中的重要阶段,总住院医师制符合临床医师培养规律,能加速提高医疗技术水平,有利于人才素质的全面提升.只有加强总住院医师阶段的培养,才能造就高质量的临床医师.     

创新军队医院聘用人员管理模式的探讨

第四军医大学拥有2所大型综合性附属医院。在军队医院编制体制调整后,医院设立了严格的人员编制定额,为维持原有医院规模并满足医院持续发展的需要,近年来陆续招聘地方医务人员及地方医学院校毕业生来我校工作。现就招     

营养元素对菘蓝生长特性的影响

目的 阐明不同营养元素对菘蓝生长的影响,明确其营养需求,为菘蓝的精准施肥提供理论依据。方法 以菘蓝幼苗为研究对象,采用水培法研究元素种类和浓度对其生长特性的影响,观察缺素处理菘蓝幼苗的典型特征,测定不同处理菘蓝生长和生理指标。结果 元素氮（N）、磷（P）、钾（K）、钙（Ca）、镁（Mg）、铁（Fe）、铜（Cu）、锰（Mn）、锌（Zn）对菘蓝幼苗生长具有关键作用。菘蓝对不同营养元素的需求程度不一,低浓度Zn、硼（B）,中浓度N、K、Mg、Fe、Mn,高浓度P、Ca、Cu对菘蓝生长有显著促进作用。结论 N、P、K、Ca、Mg、Fe、Cu、Mn、Zn元素对菘蓝生长影响较大,尤其是K、N、Fe、P元素。