生物反应器中的力学刺激促进组织工程软骨再生

BACKGROUND: Cartilage tissue engineering has been widely used to achieve cartilage regeneration in vitro and repair cartilage defects. Tissue-engineered cartilage mainly consists of chondrocytes, cartilage scaffold and in vitro environment. OBJECTIVE: To mimic the environment of articular cartilage development in vivo, in order to increase the bionic features of tissue-engineered cartilage scaffold and effectiveness of cartilage repair. METHODS: Knee joint chondrocytes were isolated from New Zealand white rabbits, 2 months old, and expanded in vitro. The chondrocytes at passage 2 were seeded onto a scaffold of articular cartilage extracellular matrix in the concentration of 1×106/L to prepare cell-scaffold composites. Cell-scaffold composites were cultivated in an Instron bioreactor with mechanical compression (1 Hz, 3 hours per day, 10% compression) as experimental group for 7, 14, 24, 28 days or cultured statically for 1 day as control group. RESULTS AND CONCLUSION: Morphological observations demonstrated that the thickness, elastic modulus and maximum load of the composite in the experimental group were significantly higher than those in the control group, which were positively related to time (P < 0.05). Histological staining showed the proliferation of chondrocytes, formation of cartilage lacuna and synthesis of proteoglycan in the experimental group through hematoxylin-eosin staining and safranin-O staining, which were increased gradually with mechanical stimulation time. These results were consistent with the findings of proteoglycan kit. Real-time quantitative PCR revealed that mRNA expressions of collagen type I and collagen type II were significantly higher in the experimental group than the control group (P < 0.05). The experimental group showed the highest mRNA expression of collagen type I and collagen type II at 21 and 28 days of mechanical stimulation, respectively (P < 0.05). With the mechanical stimulation of bioreactor, the cell-scaffold composite can produce more extracellular matrix, such as collagen and proteoglycan, strengthen the mechanical properties to be more coincident with the in vivo environment of cartilage development, and increase the bionic features. With the progress of tissue engineering, the clinical bioregeneration of damaged cartilage will be achieved.   中国组织工程研究杂志出版内容重点：组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程     

安徽省麻疹野毒株的首次分离和鉴定

为了监测安徽省现流行的麻疹野毒株,在1998、1999年的2起麻疹暴发中,用绒猴淋巴母细胞（B95a）分离到4株麻疹野病毒,通过血凝试验、血吸附试验、中和试验对分离到的麻疹病毒进行特性鉴定。该4株病毒无血凝和血球吸附活性,我国人类疫苗免疫后,血清能中和这4株病毒,但中和此野毒株的滴度低于中和疫苗株滴度的2倍。逆转录聚合酶链反应（RT－PCR）和其产物的基因序列分析提示,该4株病毒属于麻疹病毒第八基因组（Hgroup）。继续监测我国各省麻疹流行株的基因和抗原变异,对我国强化麻疹控制及麻疹消除、消灭十分必要     

加权平均组合评价法及其应用

目的针对现有的组合评价方法的不足,提出一种改进的组合评价方法--加权平均组合评价法.方法通过实例介绍加权平均组合评价法的具体步骤,并比较该法与传统的四种组合评价方法的评价效果.结果加权平均组合评价法保距效果更佳.结论加权平均组合评价法有应用推广的价值.     

研究调肝泻火法对糖尿病患者颈动脉硬化的影响

目的:研究调肝泻火法对2型糖尿病患者颈动脉硬化的影响。方法:将149例患者按完全随机原则分为中药组、西药组及空白对照组,空白对照组予基础治疗,中药、西药组在空白对照组基础上分别予金柴消渴胶囊、盐酸吡格列酮,3个月1个疗程,观察2个疗程。结果:3组治疗前后血脂自身前后对比,P0.01,中药组与基础治疗组对比,P0.05,西药组与基础治疗组相比P0.05.3组治疗前后IMT均有显著改善P0.01;中药组较基础治疗组改善明显P0.05,中药组与西药组比较,西药组与基础治疗组比较,P0.05。结论:以调肝泻火法组方的金柴消渴胶囊可使糖尿病患者的血脂改善,并有抗动脉粥样硬化作用,进而使颈动脉IMT增厚减退。     

Heart Rate Distribution and Cause-specific Death in General Population of South China

Based on the development of pathologic physiologyprocess of coronary heart disease, it is considered thatlower heart rate(HR) is advantageous to the protectionof ischemic heart and the treatment of heart failure.Thus traditional normal range of HR from 60 to 100beats /min (bpm) in adult should be questioned. Thistext analyzed the baseline and follow-up data of thePRC-USA Collaborative Study of CardiovascularEpidemiology to investigate the HR distribution inhealthy community adults and to…     

工业氟污染区人群骨骼X线的改变

随着改革开放进一步深入和中央西部大开发号召 ,各地引资办厂、办企业如雨后春笋 ,但如何保护好环境 ,减少工业污染 ,值得人们高度重视。现将某化肥厂工业废水、废气排放污染区12 0人骨骼 X线改变观察结果报告如下。1　材料与方法1.1　对象选择　某化肥厂建于 80年代初 ,投产后排污废水地段的耕作地长期受到污灌 ,在距厂排污下段 10 0 0 m村寨 (土壤氟含量 132 1.2 9mg/ kg,水氟含量 0 .44～ 9.6 3mg/ L ,大气氟含量0 .0 199～ 0 .0 15 9mg/ m3 ,食物氟含量3.0 0～ 5 3.6 6 mg/ kg)抽取 15～ 73岁12 0人作为观察组。选择土壤氟含量885 .6 7…     

调肝泻火法对糖尿病颈动脉硬化患者胰岛素抵抗的影响

目的研究调肝泻火法对2型糖尿病颈动脉硬化患者胰岛素抵抗的影响。方法将149例2型糖尿病患者按完全随机、单盲分为3组,对照组49例子降糖、降脂配合饮食、运动等基础治疗,中药组50例在对照组治疗基‘础上予金柴降糖胶囊治疗,西药组50例在对照组治疗基础上给予吡格列酮治疗。观察3组总胆固醇（TC）、三酰甘油（TG）、低密度脂蛋白（LDL）、高密度脂蛋白（HDL）、白细胞介素-6（IL-6）、c反应蛋白（CRP）、肿瘤坏死因子-α（TNF—α）、胰岛素抵抗指数（HOMA—α）及胰岛素分泌指数（HOMA—B）的变化。结果中药组在改善TC、TG、LDL、IL-6、TNF-α、CRP、HOMA-α、HOMA—B方面均优于对照组（P均〈0．05）,西药组在改善TC、TG、TNF—α、CPR方面优于对照组（P均〈0．05）,中药组与西药组各项指标比较均无显著性差异（P均〉0．05）。蛄论调肝泻火法可改善2型糖尿病颈动脉硬化患者胰岛素敏感性,其可能机制与其调脂及降低TNF—α与CRP水平有关。     

信息网络技术在船舶检验检疫业务中的应用

[目的]适应大通关的要求，实现船舶检验检疫工作由单一模式向信息化模式的转变。[方法]通过计算机网络技术和电信工具，使检验检疫部门与船舶代理公司之间架起信息高速公路，开发了1套集船舶电子申报、检疫、监管、监督、出证于一体的《船舶检验检疫管理系统》(以下简称系统)，开辟了船舶检验检疫新方式。[结果]该系统的运行使检验检疫与船代公司之间构筑了一条信息化高速公路，使大窑湾出入境检验检疫局船舶检验检疫工作全部实现了电子化。[结论]系统的应用，使船舶检疫工作提高了效率，加速了船舶的通关速度，减少了船舶在港的停泊时间，为船舶代理公司节约了资金，填补了CIQ2000系统缺少船舶检疫的空白。为船舶卫生检疫工作提供了便捷的工作方式。     

高热 咳嗽 气喘伴皮下气肿

患者男性,16岁,主因\     

慢性重型肝炎血清甲胎蛋白检测意义

了解血清甲胎蛋白测定在慢性重型肝炎患者药物治疗疗效、预后判断中的意义,为临床治疗、预后判断提供依据。70例慢性重型肝炎患者在院对其进行血清甲胎蛋白测定,而后观察治疗2月后的患者肝功能等恢复情况。初次血清甲胎蛋白测定浓度大于200μg／L组患者肝功能好转率明显高于（100-200）μg／L组及100μg／L以下组。血清甲胎球蛋白含量高,则其治疗后肝功能好转率高,预后好,反之则差。血清甲胎蛋白测定可作为慢性重型肝炎疗效和预后判断的指标。