银屑病易感基因HLA-Cw*0602的构建

目的扩增银屑病易感基因HLA-Cw* 0602,为进一步利用该易感基因制备银屑病转基因动物模型提供试验基础。方法以银屑病患者全血基因组为模板,用HLA-Cw* 0602特异性引物筛选含有该基因的阳性模板,根据HLA-Cw*0602基因全长序列设计引物,利用聚合酶链式反应,扩增出相应基因片段,并克隆至质粒载体pMD-19T Simple Vector。结果PCR鉴定、酶切鉴定和测序鉴定结果显示HLA-Cw* 0602基因构建成功。结论成功构建HLA-Cw* 0602基因,为后续银屑病转基因动物的制备奠定基础。     

丹参4-(5′-二磷酸胞苷)-2-C-甲基-D-赤藓醇激酶的cDNA全长克隆及其诱导表达分析

本文首次从丹参毛状根中克隆得到了丹参4-(5′-二磷酸胞苷)-2-C-甲基-D-赤藓醇激酶(简称为SmCMK)的编码基因的全长cDNA序列,GenBank注册号:EF534309。KEGG软件分析表明:SmCMK位于二萜类化合物生物合成的上游途径——非甲羟戊酸途径(DXP),是DXP途径上唯一的激酶。所克隆的SmCMK基因序列全长1 493 bp,包括完整的开放阅读框(open reading frame,ORF),推测编码396个氨基酸的多肽,分子质量为43.302 kDa,等电点(pI)值为6.78;含有71 bp的5′非转译区(5′UTR)和232 bp的3′非转译区(3′UTR)。末尾具有完整的AATAA加尾信号和PolyA结构,说明所克隆基因的序列较为完整。经序列比对分析表明,SmCMK与其他植物CMK激酶家族具有较高的同源性。实时荧光定量PCR结果显示该基因受茉莉酸甲酯(MJ)诱导后表达水平显著升高,与丹参酮类成分含量受MJ诱导后的增加趋势一致,初步证明了SmCMK基因表达量与丹参酮类成分的积累之间的关系,为进一步研究丹参酮类成分的次生代谢调控机制奠定了基础。     

糖尿病视网膜病变激光光凝对角膜内皮的影响

目的 观察糖尿病视网膜病变患者行全视网膜光凝对角膜内皮细胞的影响.方法 用角膜内皮细胞显微镜对532 nm激光行糖尿病视网膜病变全视网膜光凝的患眼进行角膜内皮细胞的观察.结果 532 nm激光全视网膜光凝后14 d及21 d中央部位及周边6点处角膜内皮细胞的各项检测指标的改变均无统计学意义（P＞0.05）.结论 532 nm激光行糖尿病视网膜病变全视网膜光凝对角膜内皮细胞造成的损伤没有统计学意义.     

掌控性爱“生物钟”

古人云：“文武之道，一张一弛。”实际上，性爱之道，也是一张一弛。循环周期在人的生理和行为上成为一种普遍的规律。人们的生活也不能例外，也要受这种循环规律的支配和制约。这一点，从女性的性欲循环周期律当中可以得到十分明显的证实。     

癫清片对戊四唑点燃大鼠海马损害的病理学观察

目的观察癫清片对戊四唑(PTZ)点燃大鼠脑组织学损害特点。方法取按照One’s分级标准造模成功的癫大鼠60只,随机分为模型对照组;癫清片高、中、低剂量组;癫宁片组及苯妥英钠片组,连续灌胃给药28 d,处死后脑标本制成4μm层厚的横轴位切片,HE染色,光镜下观察各组海马的CA1、CA2、CA3、CA4的组织学改变,并选择组织学损害最明显的区域在高倍镜下(40×10)计数神经元的丢失。结果模型对照组总的神经细胞数量减少,变性神经细胞可占锥体细胞的83%,各给药组与模型组相比,均可增加总的神经细胞数量,变性神经细胞比率减少,其中癫清片高剂量组与苯妥英钠片组表现相似。结论PTZ可使海马的CA1、CA2、CA3、CA4的大量神经细胞变性坏死;癫清片具有增加锥体层总体神经细胞和正常神经细胞数量的作用。     

水煎提取赤首乌白首乌的中试探讨

目的探讨赤、白首乌有效部位的创新水提取、分离、纯化工艺。方法主要应用生物酶工程技术与絮凝技术对水提取液作前处理,采取组合大孔树脂技术进行分离精制。结果及结论白首乌的平均提取率高,赤首乌中二苯乙烯苷含量高。     

水提何首乌有效部位重金属和农药残留的研究

采用创新中药水提取工艺,将生物酶技术、絮凝技术、组合大孔树脂技术共同用于何首乌有效部位提取、分离与精制,检测其重金属和农药残留,结果表明符合标准。     

重组链激酶溶栓联合氯吡格雷治疗急性心肌梗死的临床观察

目的观察国产重组链激酶(r-SK)治疗急性心肌梗死时经加用口服氯吡格雷后的临床疗效以及其安全性。方法自2006年10月至2007年10月间的急性心肌梗死(AMI)患者36例经国产重组链激酶溶栓联合氯吡格雷治疗的血管再通率、急性期病死率、并发症以及不良反应发生率。结果梗死相关血管再通率为86%,梗死后心绞痛6%,严重心律失常8%,心源性休克3%,出血8%,30d病死率3%。无其他药物相关的严重不良反应及过敏反应发生。结论r-SK溶栓联合氯吡格雷治疗急性心肌梗死是安全有效的。     

小儿推拿加针灸法治疗婴幼儿秋冬季腹泻

2006年10月～2007年11月收治秋冬季腹泻患儿20例，男12例，女8例，年龄4个月～2岁，病程≤2天。     

Pathological features of gastric cancer in Zhuanghe high-risk area in China during 1992–2005

Objective  To investigate the pathological features and chronological changes of 1003 cases with gastric cancer in Zhuanghe high-risk area during 1992–2005 and the relationship between the changes and etiology factors in order to make a clue for gastric cancer prevention. Methods  A total of 1003 gastric cancer specimens resected surgically between 1992–2005 in Zhuanghe Center Hospital were studied. The specimens were fixed in formalin and diagnosed by routine pathology. Results  The incidence of patients with gastric cancer was highest at age of 60–69, the next high was at age of 50–59 and it was significantly higher in male than in female (P<0.001), the ratio was 3.0:1. During the past 14 years, there were 159 (15.9%) EGC, 195 (19.4%) moderate and 649 (64.7%) advanced gastric cancer detected. In macroscopical features, type III remained dominant in EGC, the next was mixed type in EGC. In advanced gastric cancer the Borrmann’s type III remained the dominant, the next was type II. For nodal metastasis, positive cases were decreasing and negative were increasing in EGC, moreover negative cases were higher than positive ones each year. There was no obvious trend in advanced cancer but positive cases were higher than negative ones each year. In histological features, papillary, moderately and poorly differentiated tubular adenocarcinoma remained downtrend, mucus adenocarcinoma and undifferentiation cancer remained uptrend year after year. The radio of intestinal to diffuse type decreased from 0.78 to 0.62 during the past 14 years. Conclusion  There were significant chorological trends of pathological characteristic of gastric cancer in Zhuanghe high-risk area during the past 14 years. This work was supported by the National “Tenth-Five” Key Technologies R&D Program of China (2004BA703B04-02)