Survivin在人胃腺癌中的表达及其与临床病理的关系

目的：研究Survivin蛋白在人胃腺癌细胞系和组织中的表达及其临床意义。方法：用RT-PCR、Western Blot方法及免疫组织化学方法检测6株人胃腺癌细胞系,80例人胃腺癌及癌旁组织中Survivin基因的表达。结果：Survivin在6株人胃腺癌细胞系均有表达,胃腺癌组织中Survivin表达阳性率为60%,癌旁正常胃组织均无Survivin的表达;在胃腺癌中的表达与患者生存期和肿瘤的区域淋巴结转移（P〈0.05）密切相关,与肿瘤分化程度无相关性（P〉0.05）。结论：Survivin的变化与胃腺癌的发生和预后密切相关,并可能成为一个有希望的肿瘤治疗靶点。     

几种不同液体复苏失血性休克大鼠的适宜量研究

目的对几种不同液体复苏失血性休克大鼠适宜量进行探讨。方法SD大鼠分别做35%、45%放血失血性休克模型,以不同量的乳酸林格液(LR)、羟乙基淀粉(HES)、高渗氯化钠右旋糖酐(HSD)和LR HES进行复苏,观察其对失血性休克大鼠平均动脉血压(mean arterial blood pressure,MAP)、左心室收缩压(left intraventricular systolic pressure,LVSP)、左心室压力上升或下降的最大速率(the maximal change rate of left intraventricular pressure,±dp/dtmax)的影响,同时观察血气指标变化和存活率的变化。结果在35%失血性休克(中度休克)时,不同剂量的LR、HSD输注对休克大鼠血流动力学的影响无显著差异(P>0.05);30ml/kg HES输液后血流动力学指标明显优于20ml/kg HES(P<0.05);1倍失血量LR HES输注优于2倍和3倍失血量的LR HES(P<0.05)。在45%失血性休克(重度休克)时,3倍失血量LR输液后血流动力学指标明显比1倍和2倍失血量的LR效果好(P<0.05);6ml/kg的HSD输注后血流动力学效果比4、8ml/kg的HSD效果好(P<0.05)。同样,30ml/kg HES和2倍失血量的LR HES输液后改善血流动力学指标,血气指标和存活时间效果优于其他剂量组的HES和LR HES(P<0.05),除HSD各剂量组明显降低血pH值外,其他各液体对血气指标的影响均不大。结论LR除复苏重度休克以3倍失血量输注效果好外,其余液体以HES30ml/kg,HSD6ml/kg以及2倍失血量的LR HES复苏失血性休克效果较好,是较理想的失血性休克复苏容量。     

护理质量敏感指标在骨科患者护理管理中的应用

目的护理质量敏感指标在骨科患者护理管理中的应用效果。方法以本院2017年3月-2018年3月骨科收治的110例患者为研究对象,按照数字随机分配原则将所有骨科患者分为对照组与实验组,每组骨科患者55例,对照组实施常规护理方式,实验组在对照组基础上增加护理质量敏感指标管理项目,对比两组患者临床护理效果。结果实验组护理人员护理质量评估情况优于对照组,差异具有统计学意义(P0.05);实验组护理满意度高于对照组,差异具有统计学意义(P0.05)。结论在骨科患者护理管理中应用护理质量敏感指标,可以获得令人满意的护理效果,对患者身体状况的恢复、骨科临床护理效果的提升具有良好作用,因此,值得推广与应用。     

限制性液体复苏对肝脏缺血-再灌注损伤的影响

Objective To investigate the effects of limited resuscitation on hepatic ischernia-reperfusion in-jury in rots with hemorrhagic shock. Method Uncontrolled hemorrhagic shock was induced in 54 rats by transect-ing the middle branch of the splenic artery to produce standardized massive splenic injury. Resuscitation started when the mean arterial pressure (MAP) reached 40 mmHg. The rats were randomly divided (n = 9per group) in-to sham-operated group (SS), or one of five treatment groups in which infusion of Ringer' s solution was continually administrated to maintain MAP at 40 (RS40), 50 (RS50), 60 (RS60), 80 (RS80) or 100 mmHg (RS100) for 45 minutes (T45 point). After the bleeding was controlled, resuscitation was continued with Ringer's solution and whole blood (2:1) to increase the MAP to 100 mmHg for 120 minutes (T165 point), which was followed by obser-vation for 240 minutes (T405 point). All animals were observed for 240 minutes or until death. Blood specimens were collected at TO, T45, T165 and T405 for determination of blood lactate levels. At the end of the experiment,a small amount of hepatic tissue was collected to measure tissue blood perfusion, total antioxidative capacity (TAOC), Na+K+ ATPase activity and malondialdehyde (MDA) levels. Results At T405, the blood lactate lev-els in the RS80 and RS100 groups [(3.60±0.68) and (3.84 ± 1.09) mmol/L, respectively] were significantly higher than those in the SS, RS40, RS50 and RS60 groups [(2.00±0.66),(2.74±1.45),(2.43 +0.94) and (2.07±0.95) mmol/L, respectively;all:P < 0.05]. The MDA levels were significantly higher in the RS80 and RS100[(7.32±0.31) and (7.71±0.23) nmol/mg,respectively] than those in the SS, RS40, RS50 and RS60 groups[(4.95±0.80),(6.14±0.94),(6.42±0.48) and (6.84±0.36) nmol/mg, respeetively;all: P <0.05]. The Na+ K+ ATPase and TAOC levels were significantly lower in all of the RS groups than those in the SS group (all: P < 0.05), and those in the RS80 and RS100 groups was significantly lower than those in the RS40,RS50 and RS60 groups (all: P <0.05). Blood perfusion in the RS80 and RS100 groups was significantly lower than that in the other groups (all: P < 0.05). Conclusions If hemorrhage is uncontrolled, limited resuscitation appears to balance the needs for organ perfusion, improve the microcircttlation and decrease lactate levels. Fur-thermore, limited resuscitation could decrease ischemia-reperfusion injury in liver tissue.     

内毒素对鼠肺／肠微血管及体外培养内皮细胞通透性的影响及凋亡在其中的作用

目的观察内毒素（LPS）对鼠肺和肠微血管及体外培养内皮细胞通透性的影响及凋亡在其中的可能作用。方法（1）观察内毒素血症时大鼠肺、肠组织微血管通透性的改变。（2）观察LPS对体外培养人脐静脉内皮细胞（HUVECs）通透性的影响。（3）观察LPS对体外培养HUVEC凋亡的影响，从而探讨凋亡在内皮细胞通透性改变中的作用。结果（1）在大鼠内毒素血症模型中，5ms／ks和10mg／kg LPS均可造成肺、回肠组织伊文氏兰含量增加（P〈0．01或0．05）。（2）100μg／ml LPS可显著增加体外单层HUVEC对牛血清白蛋白的通透性（P〈0．05或0．01）。（3）LPS可引起的体外培养内皮细胞的凋亡。结论（1）LPS能迅速提高体内肺微血管内皮细胞通透性，而LPS作用后6h肠微血管通透性开始增加。（2）LPS可剂量依赖性的引起体外培养脐静脉内皮细胞通透性升高。（3）细胞凋亡可能是引起内毒索血症时内皮细胞通透性的原因之一。     

不同液体对失血性休克大鼠复苏效果的影响

目的 探讨不同液体输注对失血性休克大鼠复苏效果的影响.方法 用SD大鼠制作35%放血失血性休克模型,以乳酸林格氏液(LR)、羟乙基淀粉130(HESl30)、6%右旋糖酐40、高渗氯化钠右旋糖酐(HSD)、LR+右旋糖酐(体积比2:1)和LR+6%HES(体积比2:1)复苏休克动物至适宜血压(80mmHg)1 h后,观察其对失血性休克大鼠平均动脉血压(mean arterial blood pressure,MAP)、左心室收缩压(left intraventricular systolic pressure,LVSP)、左心室压力上升或下降的最大速率(the maximal change rate of left intraventricular pressure,±dp/dtmax)的影响,同时观察血气指标和存活时间的变化.结果 输注LR、HES130、LR+6%HES后MAP能较好地维持在80 mmHg水平,而右旋糖酐40、LR+右旋糖酐以及HSD输注后MAP不能达到80 mmHg.HES130、LR+6%HES输注后LVSP和±dp/dtmax明显增加,而输注右旋糖酐、LR+右旋糖酐以及HSD后,LVSP和±dp/dtmaxLR组显著降低(P<0.05),以输注HSD后最低.输注HSD后动物存活时间最短,输注HES130和LR+6%HES后动物存活时间较其他组长(P<0.05).HES130、LR+6%HES改善血气效果较其他各组好.结论 不同液体对失血性休克大鼠复苏效果存在明显差异,以HES130和LR+6%HES效果较好,HSD效果较差.     

限制性液体复苏对肝脏缺血-再灌注损伤的影响

Objective To investigate the effects of limited resuscitation on hepatic ischernia-reperfusion in-jury in rots with hemorrhagic shock. Method Uncontrolled hemorrhagic shock was induced in 54 rats by transect-ing the middle branch of the splenic artery to produce standardized massive splenic injury. Resuscitation started when the mean arterial pressure (MAP) reached 40 mmHg. The rats were randomly divided (n = 9per group) in-to sham-operated group (SS), or one of five treatment groups in which infusion of Ringer' s solution was continually administrated to maintain MAP at 40 (RS40), 50 (RS50), 60 (RS60), 80 (RS80) or 100 mmHg (RS100) for 45 minutes (T45 point). After the bleeding was controlled, resuscitation was continued with Ringer's solution and whole blood (2:1) to increase the MAP to 100 mmHg for 120 minutes (T165 point), which was followed by obser-vation for 240 minutes (T405 point). All animals were observed for 240 minutes or until death. Blood specimens were collected at TO, T45, T165 and T405 for determination of blood lactate levels. At the end of the experiment,a small amount of hepatic tissue was collected to measure tissue blood perfusion, total antioxidative capacity (TAOC), Na+K+ ATPase activity and malondialdehyde (MDA) levels. Results At T405, the blood lactate lev-els in the RS80 and RS100 groups [(3.60±0.68) and (3.84 ± 1.09) mmol/L, respectively] were significantly higher than those in the SS, RS40, RS50 and RS60 groups [(2.00±0.66),(2.74±1.45),(2.43 +0.94) and (2.07±0.95) mmol/L, respectively;all:P < 0.05]. The MDA levels were significantly higher in the RS80 and RS100[(7.32±0.31) and (7.71±0.23) nmol/mg,respectively] than those in the SS, RS40, RS50 and RS60 groups[(4.95±0.80),(6.14±0.94),(6.42±0.48) and (6.84±0.36) nmol/mg, respeetively;all: P <0.05]. The Na+ K+ ATPase and TAOC levels were significantly lower in all of the RS groups than those in the SS group (all: P < 0.05), and those in the RS80 and RS100 groups was significantly lower than those in the RS40,RS50 and RS60 groups (all: P <0.05). Blood perfusion in the RS80 and RS100 groups was significantly lower than that in the other groups (all: P < 0.05). Conclusions If hemorrhage is uncontrolled, limited resuscitation appears to balance the needs for organ perfusion, improve the microcircttlation and decrease lactate levels. Fur-thermore, limited resuscitation could decrease ischemia-reperfusion injury in liver tissue.     

汉黄芩苷的药理活性研究进展

综述汉黄芩苷的药理活性研究进展。汉黄芩苷是一种具有多种生物活性的黄酮类化合物,具有抗肿瘤、抗炎、抗菌、抗氧化、心血管保护、神经保护等多种药理活性作用,分析其作用机制为更加深入地开发利用汉黄芩苷提供了科学依据。     

调控RhoA/Rac平衡对失血性休克大鼠血管双相反应性的影响

目的 观察调控RhoA/Rac平衡在休克后血管反应性双相变化中的作用.方法 采用失血性休克模型,分别取RhoA、Rac激动剂或抑制剂,观察其对休克早期或休克晚期大鼠平均动脉血压(mean arterial pressure,MAP)、肠系膜上动脉血管管径对去甲肾上腺素(norepinephrine,NE)收缩反应性的影响.结果 失血性休克后整体动物肠系膜上动脉对NE的收缩反应和NE的升压反应呈双相变化,休克早期升高,休克晚期降低.Rac激动剂血小板衍生生长因子(platetederived growth factor,PDGF)和RhoA特异性抑制剂C3转移酶可降低休克早期肠系膜动脉对NE收缩反应性和NE升压反应性;RhoA激动剂U-46619和Rac特异性抑制剂NSC23766可改善休克晚期肠系膜上动脉对NE的收缩反应和NE的升压效果,也进一步升高休克早期收缩反应性;RhoA激动剂U-46619和Rac激动剂PDGF的作用可以分别被其特异性抑制剂所拮抗.结论 用RhoA或Rac激动剂或拮抗剂可调控失血性休克大鼠血管反应性双相变化,提示休克早期RhoA活性升高,Rac活性降低,而休克晚期RhoA活性降低,Rac活性升高,RhoA/Rac活性平衡参与了休克后血管反应性双相变化的调节.