蒙医针刺疗法结合微波治疗周围性面神经麻痹的疗效观察

目的:观察蒙医针刺疗法结合微波治疗周围性面神经麻痹的临床疗效。方法:72例周围性面神经麻痹的患者随机分为两组,即对照组(以激素为主的西药治疗组)和治疗组(蒙医针刺结合微波治疗组),每组36例,两组进行同步治疗,疗程不超过3次(每个疗程为10天)。治疗结束后,对两组治疗积分和总疗效进行对比分析。结果:①治疗组治疗后计分为0.14±0.2,对照组为0.80±0.1,两组比较有显著性差异(P<0.05);②治疗最多3个疗程后,治疗组治愈率为88.88%;对照组治愈率为61.11%(P<0.05)。结论:蒙医针刺结合微波治疗周围性面神经麻痹可以提高治愈率,降低治疗后积分,提示蒙医针刺结合微波治疗周围性面瘫的疗效较以激素为主的西药治疗明显,是治疗周围性面神经麻痹的有效方法之一,值得临床推广。     

2012年北京雨洪灾害后传染病疫情风险评估与应对策略

目的分析北京市2012年7·21洪涝灾害后存在的主要危险因素,确定传染病疫情风险评估结果,并提出应对策略。方法运用历史数据分析、文献法和专家咨询法以及现场观察,开展风险识别和客观评价,并利用矩阵法进行风险等级评估,确定洪涝灾后可能的传染病疫情和公共卫生风险,并制定管理策略。结果综合分析北京市2012年7月21日雨洪灾害后传染病发生的主要危险因素,确定北京市雨洪灾害后需重点防控的传染病主要包括肠道传染病(8种)、自然疫源性疾病(2种)、虫媒传染病(乙脑)以及狂犬病等12种疾病。并树立"大卫生"观念,制定出切实可行的风险控制管理策略。结论这些风险评估的结果和管理策略为政府决策提供了科学依据,落实到了灾后疫情应急管理的每个环节,为此次洪灾后的风险管理做到了有的放矢,"关口前移",实现了"大灾之后无大疫"的目标。     

西洋参茎叶化学成分研究进展

本文查阅了国内外西洋参茎叶相关文献,按其化学结构进行分类,对西洋参茎叶的化学成分作一综述。西洋参茎叶的主要化学成分是三萜皂苷类、黄酮、挥发油和木脂素等。希望此研究能为西洋参和西洋参茎叶的研究开发与深加工提供参考。     

阴囊部鲍温样丘疹病演变为疣状癌1例

阴囊部鲍温样丘疹病演变为疣状癌１例李铁男，刘岩，顾苏宁，李映菊沈阳市第七人民医院皮肤科（邮政编码１１０００３）患者男，３２岁。半年前于阴囊右侧发生数个粟粒大小暗红色丘疹，不痛，不痒。以后皮疹增多，阴囊左侧也出现同样皮疹。皮疹逐渐增大。曾在外院以“血管...     

HSV-TK/GCV自杀基因系统治疗小鼠乳腺癌的研究

目的:探讨HSV-TK/GCV自杀基因系统对小鼠乳腺癌细胞系MA782/5S-8102形成肿瘤的体内杀伤作用及其产生的旁观者效应。方法:体内实验分动物致瘤组、抑瘤实验组和治疗实验组。分别按实验组别要求接种5.0×106细胞/只于BALB/C小鼠的右腋窝皮下,观察各组肿瘤形成及肿瘤治疗情况并统计各组生存期。治疗结束后将标本制成石蜡切片进行病理学分析,RT-PCR检测HSV-TK基因在肿瘤组织中的表达情况。统计学处理采用SPSS软件进行完全随机的方差分析(ANOVA)。结果:实验结果显示小鼠成瘤率为100％。丙氧鸟苷(ganciclovir,GCV)可明显抑制MA782/5S-8102/TK细胞在BALB/C小鼠体内的肿瘤形成。经GCV治疗后,MA782/5S-8102/TK组和混合细胞组的肿瘤体积分别较对照组肿瘤体积缩小约36.7％和28.6％(均P<0.001);生存期也明显延长(P<0.001);RT-PCR检测HSV-TK基因在肿瘤组织中有表达。实验组肿瘤组织与对照组相比存在明显的病理学改变。结论:逆转录病毒可介导HSV-TK基因转入小鼠乳腺癌细胞MA782/5S-8102并获稳定表达,HSV-TK/GCV自杀基因系统在体内对乳腺癌细胞有杀伤作用,且存在明显的旁观者效应。     

陈苏宁开玄府法治疗功能性消化不良经验总结

中医理论认为,五脏六腑皆有玄府,而玄府更是气机升降出入的基本通道。上中下三焦、脏腑内外、经络气血皆依靠玄府进行基本物质交换。玄府通则百病除,玄府闭则百病生。在脾胃病治疗上,玄府同样对中焦运化起着重要作用。功能性消化不良即为玄府郁闭的典型表现。陈苏宁教授应用玄府理论,采取开玄府法治疗功能性消化不良。其认为功能性消化不良关键为气机不畅,升降失和,治疗上依据中医证候辨证用药,同时考虑到玄府郁闭的基本病机,在辨证基础上配合中药宣剂开通玄府,以畅达中焦,开郁解闭。为中医临床治疗功能性消化不良提供新的思路。     

一株新的急性髓系白血病细胞系SH-2的建立及其鉴定

Objective To establish and characterize a novel human myeloid leukemia cell line SH-2. Methods Bone marrow mononuclear cells(BMMNC) isolated from a AML-M2 patient, who failed to ob-tain complete remission after chemotherapy and allogenic bone marrow transplantation were passed in a long term IMDM culture medium supplemented with 20% fetal calf serum. Stromal cells were retained and rh-IL-3was added in the culture system. A new human myeloid leukemia cell line SH-2 was successfully established with a cytogeuetic characteristics of a loss of Y chromosome(- Y), a derivative chromosome 16 resulting from unbalanced translocation between chromosome 16 and 17, monosome 17, trisomy 19 and p53 alteration. Vari-ous methods were employed to characterize SH-2 cell line. Results SH-2 cells has been maintained without cytokine and stromal cells for more than 3 years without EB virus and mycoplasma contamination. SH-2 cells had the basically same morphological, immunophenotypic and cytogenetic features as the patient' s leukemia cells did, such as myeloid morphology, an immunophenotype of CD13+ , CD33+ , CD56+ , CD16/56+ and a hypodiploid karyotype of 45, X, - Y, der(16) t(16;17) (q24;q12) , - 17, + 19, which were gradually de-creased and replaced by the near-tetraploid cells with a karyotype of 73 - 102 (80), XX, - Y, - Y, del (lq31) ×2, der(16)t(16;17) (q24;q12) ×2, - 17, - 17, + 19, + 19. FISH and multiple FISH delineated all the abnormalities and revealed a loss of one p53 allele due to monosomy 17. DNA direct sequencing detec-ted a point mutation of CAG to CAT at codon 576 of exon 5 in another p53 allele. RT-PCR showed that SH-2 cells expressed apoptosis-related genes (bcl-2, Fas, GST- π and p21) rather than MDR-related genes. Short tandem repeat PCR provided powerful evidence for the derivation of SH-2 cell line from the patient' s leukemia cells. SH-2 cells had certain colony formation and tumorigenic capacities in nude and SCID mice. Conclu-sion SH-2 is a new myeloid leukemia cell line with a unique biology background, and will provide a useful tool for leukemia research.     

异基因造血干细胞移植治疗复发难治性急性淋巴细胞白血病的临床研究

目的 探讨异基因造血干细胞移植(allo-HSCT)对复发难治性急性淋巴细胞白血病(ALL)患者的疗效及治疗相关毒性.方法 观察并分析47例复发难治性ALL患者对allo-HSCT治疗的耐受情况、移植相关并发症、总生存率以及无病存活率.其中HLA相合同胞间移植(sib-HSCT)19例,HLA相合的无血缘关系移植(URD-HSCT)18例,单倍型移植(Hi-HSCT)10例.预处理方案:42例采用改良TBI+CY方案,5例采用改良BU/CY方案.移植物抗宿主病(GVHD)的预防:环孢素(CsA)联合短程甲氨蝶呤(MTX)、Hi-HSCT和URD-HSCT加用霉酚酸酯(MMF)及抗胸腺细胞免疫球蛋白(ATG).定期监测微量残留病变(MRD),明确有分子生物学或细胞遗传学复发趋势的患者接受供者淋巴细胞输注(DLI).结果 所有患者均完成移植治疗,出现了不同程度黏膜炎;2例患者在应用CsA过程中有肾功能损害;1例患者发生药物性癫痢.移植后出现Ⅲ～Ⅳ度急性GVHD 7例;慢性GVHD22例;致命性肺部感染9例(包括间质性肺炎3例);出血性膀胱炎4例.有13例患者移植后再次复发.移植后造血重建的中位时间为移植后第17天.术后有19例接受DLI,6例疾病未再进展.中位随访期43(10～77)个月,预期5年总生存率为49.65%,无病存活率为46.55%.结论 统 allo-HSCT能有效治疗复发难治性ALL,改善其预后,治疗失败的主要原因是移植后复发,其次为致命性肺部感染和重度急性GVHD.DLI可能有助于减少移植后复发.     

医学期刊综合质量评价的实践与思考

通过对中华医学会系列期刊进行政治、学术、编辑出版及运营质量全面考评,探讨科技期刊评价和质量控制体系的建立。     

一株新的急性髓系白血病细胞系SH-2的建立及其鉴定

Objective To establish and characterize a novel human myeloid leukemia cell line SH-2. Methods Bone marrow mononuclear cells(BMMNC) isolated from a AML-M2 patient, who failed to ob-tain complete remission after chemotherapy and allogenic bone marrow transplantation were passed in a long term IMDM culture medium supplemented with 20% fetal calf serum. Stromal cells were retained and rh-IL-3was added in the culture system. A new human myeloid leukemia cell line SH-2 was successfully established with a cytogeuetic characteristics of a loss of Y chromosome(- Y), a derivative chromosome 16 resulting from unbalanced translocation between chromosome 16 and 17, monosome 17, trisomy 19 and p53 alteration. Vari-ous methods were employed to characterize SH-2 cell line. Results SH-2 cells has been maintained without cytokine and stromal cells for more than 3 years without EB virus and mycoplasma contamination. SH-2 cells had the basically same morphological, immunophenotypic and cytogenetic features as the patient' s leukemia cells did, such as myeloid morphology, an immunophenotype of CD13+ , CD33+ , CD56+ , CD16/56+ and a hypodiploid karyotype of 45, X, - Y, der(16) t(16;17) (q24;q12) , - 17, + 19, which were gradually de-creased and replaced by the near-tetraploid cells with a karyotype of 73 - 102 (80), XX, - Y, - Y, del (lq31) ×2, der(16)t(16;17) (q24;q12) ×2, - 17, - 17, + 19, + 19. FISH and multiple FISH delineated all the abnormalities and revealed a loss of one p53 allele due to monosomy 17. DNA direct sequencing detec-ted a point mutation of CAG to CAT at codon 576 of exon 5 in another p53 allele. RT-PCR showed that SH-2 cells expressed apoptosis-related genes (bcl-2, Fas, GST- π and p21) rather than MDR-related genes. Short tandem repeat PCR provided powerful evidence for the derivation of SH-2 cell line from the patient' s leukemia cells. SH-2 cells had certain colony formation and tumorigenic capacities in nude and SCID mice. Conclu-sion SH-2 is a new myeloid leukemia cell line with a unique biology background, and will provide a useful tool for leukemia research.