miR-374a在糖尿病肾病患者血清中的表达水平及其意义

目的 探讨miR-374a在糖尿病肾病(DN)患者外周血中的表达及对炎症的调控机制.方法 选择2017年1月-2020年1月河北省保定市第一中心医院收治的44例T2D患者为研究对象(T2D组).根据是否并发DN,将患者分为无DN组(n=27)及DN组(n=17).根据疾病严重程度,将DN组患者分为大量蛋白尿组(n=10...     

大鼠肠道菌群对长梗冬青苷体外代谢的影响

摘 要 目的：探究体外孵育的大鼠肠道菌群对长梗冬青苷的代谢转化规律。方法: 将长梗冬青苷与大鼠肠道菌群在厌氧条件下共孵育,分别于0,4,8,12,24,48 h时取样,经乙酸乙酯萃取后采用HPLC法进行定性和定量分析。结果: 在与大鼠肠道菌群共孵育48 h后,90.8%的长梗冬青苷被代谢转化为M₂,M₂与铁冬青酸对照品通过HPLC分析时色谱行为一致,故确定M₂为铁冬青酸。结论:长梗冬青苷可以被体外孵育的大鼠肠道菌群代谢转化为铁冬青酸。     

手术前后C-肽水平及胰岛素敏感指数测定的临床价值

目的探讨手术前后病人空腹血清C-肽(C-P)、血糖(GLU)和胰岛素(INS)的关系,了解病人术后胰岛素抵抗程度和胰岛细胞功能变化。方法70例非糖尿病患者,于手术前、后各取标本1次,标本离心后每份待测标本分为2份,1份直接测定葡萄糖、胰岛素含量,另1份贮存于-20℃低温冰箱,用于测定C-肽含量。计算其胰岛素敏感指数(IAI)。结果手术后IAI水平明显下降,C-P、GLU、INS明显增高(P<0.05)。结论术后IAI水平明显下降,C-P、GLU、INS明显增高,从而导致胰岛素抵抗产生,手术后适当应用胰岛素和胰岛素增敏剂是有益的。     

小儿右室流出道室性早搏10例分析

目的　分析小儿右室流出道室早,为临床识别和治疗提供参考。方法　根据心电图特征,选出起源于右室流出道室早10例,分析其病因、临床特征、QT离散度和晚电位结果。结果　本型室早多表现为频发、连发或短阵室速,病程长,药物治疗效果不佳,运动后室早常增多,但不转为持续性室速。结论　右室流出道室早为一顽固性室早,病程长,药物治疗效果不佳,需长期随诊观察     

LASER-BEAM LITHOTRIPSY FOR BILE DUCT STONE VIA PERCUTANEOUS-TRANSHEPATIC CHOLAN- GIOSCOPY

Nd-YAG laser exposure via percutaneous-trans hepatic cholangioscopy (PTCS) has been used suc cessfully in destruction of bilirubin stones in three patients with huge commen bile duct (CBD) stones or intrahepatic stones, which couldn't be removed by endoscopic sphincteropapillotomy (EST). Experi- mental studies were conducted to observe the changes in temperature of stone surface and the surroundings in air or water, during laser cxposur:. In addition, the correlation between the elevation and recovery of temperature of the surrounding water and the speed of water infusion was observed. A plastic tube of 6 mm in diameter was used as a model. The peak temperature of stone surface 5 mm close to the con. tact point of the quartz fiber at point A, B and C was 5I C, 30 C, and 158 C respectively. No changes in temperature were observed at the three points when a similar experiment on the bilirubin stone was done but in a beaker with 400 ml water. The relationship between the peak of temperature and the speed of water infusion was not significant, but the time need ed for lowering a peak temperature to the original, after laser beam was delivered, was obviously related to the speed of water infusion. It should be em phasized that during the treatment of the biliary stone by Nd-YAG laser via PTCS, the water infusion at any time should be continued and it is better to keep the speed of water infusion not less than 40 ml/min. Of course, the free tip of the quartz fiber should be well contacted with the surface of the stone, so as to avoid a direct burn injury to the wall of the bile duct by laser beam.     

谷氨酸对原代培养海马神经细胞兴奋毒作用的研究

为更清楚地了解药物对神经元的损伤和保护作用，在体外对新生大鼠（０－１ｄ）海马神经细胞进行原代培养，建立了谷氨酸对原代培养海马神经细胞兴奋性神经毒损伤模型。通过检测神经细胞乳酸脱氢酶泄漏率和相关显微镜下对神经细胞形态变化的观察，发现只有发育成熟的神经元对谷氨酸兴奋毒损伤最敏感，神经元的兴奋毒损伤对谷氨酸在一定范围内有剂量信赖性或接触时间依赖性关系，提示谷氨酸通过与其受体结合造成神经元兴奋毒损伤。     

ELISA和PCR两种方法检测TTV感染的结果比较

目的:通过EUSA和PCR方法检测TTV。分析两种方法检测结果的差异。方法:采用ELISA和PCR两种方法同时检测无偿献血者及非甲型～庚型肝炎患者血清标本TTV-Ab、TTV、DNA。结果:ELISA法检出率为2.63% (14/532),PCR法检出率为4.14%(22/532)。结论:ELISA和PCR两种方法检测TTV感染的结果差别具有统计学意义(P<0.05)。     

96例成人牙齿正畸的临床观察

随着社会发展和人民生活水平的提高，年青人对美的追求越来越强烈，成人正畸需求日趋增加，成年人生长发育基本完成，有许多不同于青少年的特点，因此在矫治设计和拔牙选择上也有其特殊性。我院1996—2005年共收治成年患者52例，均取得良好效果，现报告如下。     

食管粘液表皮样癌7例临床病理分析

我们复查胸科医院病理科及本教研室原诊断为食管腺癌的17例切片,发现粘液表皮样癌7例。其中食道中段2例,中下段4例,缩窄型5例。瘤体直径1.5～6.1cm,切面灰白色、质硬,5例已穿透肌