Effects of Laser Acupuncture on HSP70 Expression and Arthritic Histopathology in C57 Black Mice with Osteoarthritis

Methods： Sixty C57 black mice aged 10 weeks were divided randomly into six groups. Ten mice as normal control and fifty mice received forced running for 6 weeks as model of spontaneous osteoarthritis. In four treated groups, forty mice receiving either a 90-second treatment with a combined or single 10.6 μm laser or 650 nm laser irradiation or a sham treatment 3 times a week for 4 weeks on acupoint Dubi （ST 35） of left leg. All the mice were sacrificed and the left knee joints were removed at 20^th week. Histopathotogic changes and presence of heat shock protein （HSP） of arthritic cartilage were identified by hematoxylin and eosin stain and by immunostains of HSP 70 antibody individually from frozen sections of arthritic cartilage. Histological changes in cartilage structure were assessed and quantified with the modified Mankin scoring system. Results： The modified Mankin scores were significantly higher in the model control group （5.9±1.2） than all the other groups （P〈0.05）. Combined laser irradiation group （2.5 ± 1.4） is similar to that of normal group （2.3 ± 0.5） （P〉0.05）. But there are significant differences in single 10.6μm laser and 650 nm laser for the sham group and the model group, when compare with normal group （P〈0.05）.     

风市穴定位标准化探究

目的:比较风市穴直立位人工测量与仰卧位人工测量定位的不同,以明确定位。方法:对197名志愿者进行风市穴直立位人工测量、并对其中92名志愿者进行仰卧位人工测量,比较两种测量方法间的不同。结果:直立位人工测量结果显示,股骨大转子至腘横纹距离为(39.27±3.83)cm[男性为(39.96±4.21)cm,女性为(38.82±3.49)cm,P0.05];中指尖至腘横纹距离为(19.08±2.97)cm[男性为(18.96±3.50)cm,女性为(19.16±2.58)cm];折算后得出风市穴位于腘横纹上(9.28±1.44)寸[男性(9.07±1.64)寸,女性(9.42±1.29)寸]。仰卧位人工测量结果显示,股骨大转子至腘横纹距离为(41.89±3.88)cm[男性为(42.36±3.88)cm,女性为(41.67±3.89)cm],中指尖至腘横纹距离为(23.01±3.37)cm[男性为(22.50±3.73)cm,女性为(23.25±3.20)cm];折算后得出风市穴位于腘横纹上(10.51±1.69)寸[男性为(10.16±1.75)寸,女性为(10.67±1.63)寸]。结论:风市穴位于股部,腘横纹上9寸(髌底上7寸),髂胫束后缘。直立位简便取穴法:直立垂手,掌心贴于大腿时,中指尖所指凹陷中。仰卧位简便取穴法:仰卧平躺,掌心贴于大腿外侧中央,中指尖所指凹陷中约直下1寸处。女性风市穴定位略高于男性。     

针灸为主治疗多囊卵巢综合征致不孕症的临床及实验研究近况

综述近5年来针灸为主治疗多囊卵巢综合征致不孕症的临床试验和动物实验研究进展。发现针药联合疗法和针刺疗法较为常用,关元、中极、三阴交、足三里、子宫、肾俞等腧穴的使用频率较高。针灸疗法能够通过调节激素水平,刺激卵泡、子宫内膜的生长及卵泡的排出,从而提高妊娠率。同时,相关研究也存在取穴缺乏系统化、研究设计严谨性不足、动物实验机制研究不够深入等问题。如何筛选及优化针灸治疗方案,在遵循中医辨证论治个性治疗特色的同时做到系统化,以便于临床推广执行,仍然值得未来深入研究。     

CO2激光灸治疗不同中医证型膝骨关节炎疗效观察

目的观察CO2激光灸对不同中医证型膝骨关节炎疗效。方法将88例膝骨关节炎患者根据中医辨证分为肾虚髓亏(A组28例)、阳虚寒凝(B组34例)、瘀滞筋骨(C组26例)3组,以10.6 m CO2激光照射犊鼻穴治疗。采用WOMAC VA3.1问卷,进行疗效评价。结果除A组治疗2星期、4星期时疼痛得分及2星期时僵硬得分外,治疗后3组WOMAC量表各项得分均较治疗前显著改善(P<0.05)。治疗后B组除2星期时疼痛得分外,WOMAC量表各项得分均显著优于A组(P<0.05),其治疗2星期时功能障碍得分亦显著优于C组(P=0.036);C组在治疗4星期时WOMAC量表各项得分及随访时僵硬、功能障碍得分均显著优于A组(P<0.01)。结论 CO2激光灸可有效治疗膝骨关节炎,但对不同中医证型膝骨关节炎疗效有差异,其总体趋势是阳虚寒凝型最好,其次是瘀滞筋骨型,再次为肾虚髓亏型。