儿童巨大纵隔肿物切除2例麻醉处理及体会

例1,女患,6岁,体重16 kg.术前诊断为纵隔巨大肿物,拟剖胸探查行肿物切除术.术前检查:血压110/70 mmHg,心率120次/min;CT显示后下纵隔巨大肿物(大小为11.0 cm×9.6 cm×7.2 cm),向下侵及肝门区,心脏受压前移,右肺门增大;心电图显示窦性心动过速,心电轴右偏,右心室扩大.     

细胞蜡块的制作改进与体会

随着病理新技术的不断进展,胸、腹水等液体的诊断也需要通过免疫组化或分子病理检测来进行判读,为下一步的精准治疗提供依据.因此细胞蜡块的制作就尤为重要,细胞蜡块可以很好地保持组织细胞的完整性,细胞蜡块中细胞的分布保留与实体组织有类似的排列结构,可提高诊断的准确性,并可以重复切片进行多项组织检测.但由于胸、腹水等液体的细胞成...     

二种材料修复楔状缺损431例疗效分析

目的比较两种材料修复楔状缺损。方法选取需行楔状缺损修复治疗431例,分别用光固化复合树脂、富士（Ⅱ）玻璃离子修复根据修复后半年、1年存在率进行比较分析,评价其结果。结果富士（Ⅱ）玻璃离子修复的存在率高。结论应用两种材料充填楔状缺损,富士（Ⅱ）优于光固化复合树脂。     

骨髓单个核细胞Coombs试验阳性的血细胞减少患者补体水平变化及其意义

Objective To investigate the variation of bone marrow complement level in cytopenia pa-tients with positive BMMNC-Coombs test(CBCPC), and probe the role of complement in destroying hemato-poietic cells of CBCPC patients. Methods One hundred and twenty-four patients with CBCPC and twenty-three healthy donors as controls were enrolled in this study. The levels of CI-150, C3, C4, C5b-9 were tested with ELISA. The auto-antibodies on bone marrow hematupoietic cells (BMHC) were examined with flow cy-tometry. Results The level of C5b-9 in bone marrow(BM) of untreated CBCPC patients [(119.8 ± 54.0)μg,/L] was significantly higher than that of recovered patients [(100.7 ± 33.4) μg/L] or normal controls [(93.9 ± 28.8) μg/I.] (P < 0.05). The levels of CH50 in BM of untreated or recovered CBCPC patients [(33.3 ± 11.5) kU/L, (30.8 ± 10.3) kU/L] were significantly higher than that of normal controls [(24.1 ±6.4) kU/L] (P < 0.05). The level of C3 in BM of untreated or recovered CBCPC patients [(4.9 ± 2.2) mg/ L], (5.0 ± 3.5) mg/L] was significantly lower than that of normal controls [(7.0 ± 5.6) mg/L] (P < 0.05). The level of complement in peripheral blood was consistent with that in BM. CH50 in BM of CBCPC patients was negatively correlated with their C3 (r = - 0. 303, P = 0. 007) and positively correlated with their C5b-9(r = 0. 241, P = 0. 003) levels. The level of C5h-9 in BM of CBCPC patients was higher in the BMHC-IgM positive group [(117.6 ± 55.7) μg/L] than in the BMHC- IgM negative group [(99.2 ± 26.2)μg/L] (P < 0. 05). The positive rate of CD34+ -IgG or CD34 + -IgM of CBCPC patients was positively corre-lated with their C5 b-9 level (r = 0. 593, P = 0.000, r = 0. 326, P = 0. 049). The reticulocyte percentage (r =0. 421, P = 0.000) and serum indirect bilirubin level (r = 0. 230, P = 0. 032) of CBCPC patients were posi-tively correlated with their CHSO level. Conclusions The hematocytopenia of CBCPC patients might be re-lated to the hematopoietic cells destruction caused by auto-antibedy activated complements.     

类天疱疮样扁平苔藓1例

临床资料患者女,46岁。口腔溃疡7个月,全身丘疹伴瘙痒3个月。患者7个月前无明显诱因口腔黏膜出现水疱,疱液清,进食时容易破溃,伴有明显疼痛,当地医院给予外用药物漱口（具体药物不详）,未见明显好转。3个月前先于躯干出现粟粒至黄豆大红色丘疹、斑丘疹、丘疱疹,皮损逐渐增多,发展至全身,自觉瘙痒剧烈,无发热及关节痛。我科门诊拟“扁平苔藓”行皮肤病理检查及天疱疮抗体测定,并收入院。起病以来,因口腔内水疱破溃引起疼痛进而进食少,半年来体重下降约5kg。患者平素体健,无肝炎、结核病病史,无药物及食物过敏史,家族中无类似病例。     

甲醛致雄性大鼠脑和睾丸细胞DNA-蛋白质交联的研究

目的探讨气态甲醛致雄性大鼠脑细胞和睾丸细胞DNA-蛋白质交联（DNA-protein crosslinks,DPC）作用。方法将24只SPF级Wistar雄性大鼠随机分为4组,每组6只,分别为0.5、1.0、3.0mg／m。气态甲醛染毒组和阴性对照组。连续动态吸入染毒72h后,应用KCl-SDS沉淀法检测大鼠脑和睾丸组织DNA-蛋白质交联的含量。结果与阴性对照组比较,0.5mg／m^2气态甲醛染毒组大鼠脑和睾丸组织的DPC系数差异无统计学意义（P〉0.05）,随着甲醛浓度的增加,DPC系数逐渐升高。与阴性对照组比较,1.0、3.0mg／m^3气态甲醛染毒组大鼠脑和睾丸组织DPC系数升高,差异有统计学意义（P〈0.01）结论低浓度（0.5mg／m^3）的气态甲醛不能引起大鼠脑和睾丸组织产生DPC效应,而较高浓度（1.0、3.0mg／m^3）的气态甲醛可明显诱导其产生DPC效应,造成严重的DNA损伤。     

持久性豆状角化过度症一例

患者,女,48岁。双下肢角化性丘疹半年。皮肤科检查:双下肢多发性对称分布的棕红色角化过度性丘疹,直径约1~5 mm。皮损组织病理检查示:表皮局部呈塔尖样致密角化过度,其下方表皮萎缩,真皮浅层炎细胞浸润。诊断为持久性豆状角化过度症。给予阿维A口服及维A酸乳膏外用症状好转。     

头孢菌素在输液中的稳定性

头孢菌素在输液中的稳定性王红蕾河南省新乡市第一人民医院（４５３０００）头孢菌素为半合成抗生素，在临床抗菌药物用量中已占有相当大的比例，因而与输液配伍的稳定性更加显得重要，广大医药人员对此进行了一系列的研究，为临床安全、有效、合理使用头孢菌素，提供了可...     

骨髓单个核细胞Coombs试验阳性的血细胞减少患者补体水平变化及其意义

Objective To investigate the variation of bone marrow complement level in cytopenia pa-tients with positive BMMNC-Coombs test(CBCPC), and probe the role of complement in destroying hemato-poietic cells of CBCPC patients. Methods One hundred and twenty-four patients with CBCPC and twenty-three healthy donors as controls were enrolled in this study. The levels of CI-150, C3, C4, C5b-9 were tested with ELISA. The auto-antibodies on bone marrow hematupoietic cells (BMHC) were examined with flow cy-tometry. Results The level of C5b-9 in bone marrow(BM) of untreated CBCPC patients [(119.8 ± 54.0)μg,/L] was significantly higher than that of recovered patients [(100.7 ± 33.4) μg/L] or normal controls [(93.9 ± 28.8) μg/I.] (P < 0.05). The levels of CH50 in BM of untreated or recovered CBCPC patients [(33.3 ± 11.5) kU/L, (30.8 ± 10.3) kU/L] were significantly higher than that of normal controls [(24.1 ±6.4) kU/L] (P < 0.05). The level of C3 in BM of untreated or recovered CBCPC patients [(4.9 ± 2.2) mg/ L], (5.0 ± 3.5) mg/L] was significantly lower than that of normal controls [(7.0 ± 5.6) mg/L] (P < 0.05). The level of complement in peripheral blood was consistent with that in BM. CH50 in BM of CBCPC patients was negatively correlated with their C3 (r = - 0. 303, P = 0. 007) and positively correlated with their C5b-9(r = 0. 241, P = 0. 003) levels. The level of C5h-9 in BM of CBCPC patients was higher in the BMHC-IgM positive group [(117.6 ± 55.7) μg/L] than in the BMHC- IgM negative group [(99.2 ± 26.2)μg/L] (P < 0. 05). The positive rate of CD34+ -IgG or CD34 + -IgM of CBCPC patients was positively corre-lated with their C5 b-9 level (r = 0. 593, P = 0.000, r = 0. 326, P = 0. 049). The reticulocyte percentage (r =0. 421, P = 0.000) and serum indirect bilirubin level (r = 0. 230, P = 0. 032) of CBCPC patients were posi-tively correlated with their CHSO level. Conclusions The hematocytopenia of CBCPC patients might be re-lated to the hematopoietic cells destruction caused by auto-antibedy activated complements.     

单壁碳纳米管致小鼠肝脏组织蛋白质氧化损伤的研究

目的研究单壁碳纳米材料对小鼠肝组织蛋白质的氧化损伤作用。方法用不同剂量的单壁碳纳米管悬浮液（0．1,0．2,0．4mg／ml）对小鼠进行腹腔注射一次性染毒,7d后用2,4-二硝基苯肼比色法测定小鼠肝组织蛋白质的羰基含量,以判断蛋白质的氧化损伤程度。结果注入0．4mg／ml单壁碳纳米管悬浮液后,小鼠肝的蛋白质羰基较对照组含量呈显著升高（P〈0．05）,而在低浓度（0．1mg／ml）和中浓度（0．2mg／ml）无显著差异（P〉0．05）。结论单壁碳纳米管在高浓度（0．4mg／ml）时,能引起小白鼠肝的蛋白质氧化损伤。