Effects of Repeated Electroacupuncture on β-Endorphin and Adrencorticotropic Hormone Levels in the Hypothalamus and Pituitary in Rats with Chronic Pain and Ovariectomy

<正>Objective:To explore the mechanism of electroacupuncture(EA)-induced cumulative analgesic effects on chronic pain in rats with or without ovariectomy(OVX).Methods:A total of 110 female Wistar rats were randomized into normal control(n=10),chronic constrictive injury(CCI,n=10),CCI+EA(n=30),OVX+CCI (n=30),and OVX+CCI+EA(n=30)groups.Each of the latter 3 groups was further divided into 2 days(2d),2 weeks(2 W)and 3 weeks(3 W)subgroups,respectively(n=10 in each subgroup).The CCI pain model was established by ligature of the right sciatic nerve,and the memory impairment model duplicated by OVX.The paw withdrawal latency(PWL,pain threshold)of the bilateral footplates was detected by radiant heat irradiation,and the bilateral difference in PWL(PWLD)was used to evaluate changes in the pain reaction.Morris water maze test was conducted for evaluating the rats' learning-memory ability.EA was applied to bilateral Zusanli(ST36) and Yanglingquan(GB34)for 2 d,2 W and 3 W,respectively.Pituitary and hypothalamic p-endorphin(EP)and adrenocorticotrophic hormone(ACTH)contents were detected by immunoradioassay.Results:Compared with the CCI group,PWLD of the CCI+EA-3 W group decreased significantly(P0.05).Compared with the OVX+CCI group,PWLD of the OVX+CCI+EA-3 W group was lowered considerably(P0.05),but the value was markedly higher than its basal value and those of the normal control and CCI+EA groups(P0.05).In comparison with the sham-OVX group,the escape latency,swimming distance(SD)in the target quadrant and total SD were increased remarkably in the OVX group(P0.05),while the number of target platform crossings was decreased significantly(P0.05),suggesting an impairment of the OVX rats' learning-memory ability.In simple CCI rats, bothβ-EP and ACTH contents of the pituitary increased markedly(P0.05),and those of the hypothalamus decreased obviously compared to the normal control group(P0.05).After EA,pituitary and hypothalamic ACTH levels were significantly lowered at 2 d and hypothalamic ACTH andβ-EP contents increased obviously at 3 W in comparison with the CCI group(P0.05).In OVX+CCI rats,following EA,pituitaryβ-EP contents at 2 d,2 W and 3 W,and hypothalamicβ-EP and ACTH contents at 2 W and hypothalamic ACTH levels at 3 W increased significantly(P0.05),but hypothalamicβ-EP level at 3W decreased markedly(P0.05).The effects of repeated EA in lowering pituitary ACTH and raising hypothalamicβ-EP and ACTH levels disappeared after OVX+CCI. Conclusions:Repeated EA has a cumulative analgesic effect,which is closely associated with its effects in regulating pituitary and hypothalamicβ-EP and ACTH levels.OVX may weaken the analgesic effect of EA by affecting hypothalamic-pituitary axis activity.     

药带并用治疗痛经2169例临床观察

痛经是妇科常见病和多发病，也是妇科急症、顽症之一。多发生在月经之前、行经时、或行经之后，主要表现为小腹及腰骶部的剧烈疼痛，周身不适，常随月经周期性发作，有时因疼痛剧烈出现虚脱症状；该病不仅多见，而且还可诱发其它妇科疾病，直接影响着工作和生活；几乎每个女性一生都有一次或多次     

中西医结合治疗更年期功血的临床体会

功能性子宫出血(以下简称功血)是妇产科门诊的常见病之一，是由于内分泌失调而引起的子宫出血，属中医崩漏范畴，主要表现为月经量多，经期长，严重危害这些妇女的身心健康，单纯西医用激素等法治疗，虽有一定疗效，但不够理想，单纯中药治疗对大量出血的病人抢救也尚欠把握，近几年来本人采用中西结合治疗126例病人，疗效较佳，现将其结果总结分析如下。     

经阴道超声监测卵泡大小及子宫内膜厚度在不孕症治疗中的应用

目的探讨经阴道超声（TVS）监测卵泡大小及子宫内膜厚度状况在不孕症治疗中的价值。方法应用TVS对68例不孕症患者月经周期进行连续监测卵泡生成情况及子宫内膜变化,监测3个周期以上（采用克罗米酚＋hCG,促卵泡生长及促排卵治疗）。结果68例不孕症中治疗后排卵障碍患者中有优势卵泡且最终排卵成功42例（61.8%）,42例中有22例妊娠,受孕率达52.4%。42例排卵成功妊娠与非妊娠患者的卵泡直径及子宫内膜厚度比较差异有统计学意义（P〈0.001）。结论TVS监测卵泡发育及子宫内膜变化更实用可靠,是目前生殖医学监测排卵的首选方法。     

铁心甘草治疗痛经的临床疗效观察

将100％铁心甘草煎液与元胡止痛片对照,观察68例痛经患者的临床疗效。结果铁心甘草组痊愈率28.9％,总有效率达92.1％,疗效明显高于元胡止痛片对照组。铁心甘草对气滞血瘀、寒湿凝滞、气血虚弱、肝肾亏虚型痛经均有良好疗效,尤以气滞血瘀及气血虚弱型为佳;有良好的调经作用,但对原发性痛经与继发性痛经作用无明显差异。该药具有疗效高、作用范围广、标本兼顾、毒副作用极小的特点。     

中药周期疗法治疗乳腺增生病830例

2001～2004年,笔者用自制乳核速消Ⅰ、Ⅱ、Ⅲ、Ⅳ号胶囊治疗830例经诊断明确为乳腺增生病患者,取得了满意疗效,现报告如下。1临床资料1.1一般资料830例患者均为18～45岁非孕期女性,病程最短45天,最长15年。单侧乳房发病8例,双侧发病822例;发病部位:肿块发于外上象限者占85%,其它部位占15%。治疗组830例,对照组148例,经统计学分析,两组在年龄、病程、病情上分布相近,具有可比性(P>0.05)。1.2诊断标准依据《中医病证诊断疗效标准》[1]中乳癖的诊断标准。2治疗方法2.1组方乳核速消Ⅰ号胶囊:柴胡、郁金、制香附、三棱、莪术、赤芍、海藻、夏枯…     

针刺对缺血缺氧性脑性瘫痪幼鼠脑细胞凋亡及含水量的干预效应

目的：观察针刺治疗缺血缺氧性脑性瘫痪幼鼠脑组织含水量和细胞凋亡情况，探讨其对促进神经功能恢复的影响。方法：实验于2004-09／2005-02商丘医学高等专科学校生理学教研室神经生物学实验室进行。7d龄新生Wistar大鼠62只，随机分为3组。①模型+针刺组30只，制作缺血缺氧性脑瘫幼鼠模型后12h，采用WQ-10D1型多用电子穴位治疗仪针刺百会，曲池，内关，涌泉穴位，固定频率5Hz，连续波，持续刺激20min。间隔24h后重复进行，连续7d。②模型组20只，制作缺血缺氧性脑瘫幼鼠模型，不做针刺治疗。③正常对照组12只，不做处理。各组动物于处理后7d处死，检测其脑组织含水量[(湿重-千重)／湿重&;#215;100％]及脑细胞凋亡情况(采用HPIAS-1000型图像分析系统对原位切口末端标记切片进行扫描，分析各组吸光情况，吸光度越高，说明表达越强)。结果：62只幼鼠均进入结果分析。①脑组织含水量测定结果：正常对照组平均为(79．3&;#177;2．5)％。模型+针刺组明显低于模型组[(82．2&;#177;1．9)，(85．6&;#177;2．7)％，F=9．32，P&;lt;0．05]。②细胞凋亡检测结果：正常对照组鼠脑内基本没有凋亡细胞。模型+针刺组吸光度明显低于模型组吸光度(0．203&;#177;0．065，0．254&;#177;0．071，t=2．916，P&;lt;0．05)。结论：针刺可以通过减轻脑组织水肿和减少脑细胞凋亡两方面来促进缺血缺氧性脑瘫幼鼠神经功能的恢复。     

三胶固冲汤治疗崩漏200例

自2000年以来,笔者运用贾占清主任自拟的三胶固冲汤治疗崩漏200例,疗效满意,现介绍如下。1一般资料200例崩漏病人全部为门诊患者,其中已婚125例,未婚75例;年龄最小11岁,最大52岁,11~20岁32例,21~30岁37例,31~40岁39例,41~52岁92例。排除生殖器肿瘤,凝血机制障碍性出血,炎症等器     

综合疗法治疗腓总神经麻痹的临床观察

目的:观察电针、灸法与走罐联合应用与西药对比治疗腓总神经麻痹的临床疗效差异。方法:将90例腓总神经麻痹患者随机分为综合组和西药组,各45例。综合组应用电针阳陵泉、足三里、悬钟、解溪、太冲、足临泣等穴并结合温和艾条灸以及患肢小腿外侧走罐3种方法综合治疗,每日1次;西药组应用药物口服维生素B1每次10mg,每日3次,甲钴胺每次0.5mg,每日3次。两组均15天为一疗程,2个疗程后观察疗效。结果:综合组治疗后感觉、运动神经功能改善,总有效率为97.8%(44/45),西药组为82.2%(37/45),综合组疗效优于西药组(P0.01)。电生理检查显示综合组腓深神经运动传导和腓浅神经感觉传导波幅治疗后较治疗前有明显提高(均P0.05),综合组较西药组治疗后腓深神经运动传导波幅有显著改善(P0.05)。结论:电针、灸法与走罐综合疗法治疗腓总神经麻痹较西药治疗疗效显著。     

CLINICAL APPLICATION OF NEIGUAN （内关 PC 6）IN THE TREATMENT OF DISORDERS OF THE DIGESTIVE SYSTEM THE RELATED RESEARCH PROGRESS

In the present paper, the authors review recent situations of clinical application of Neiguan (PC 6 内关), etc. in the treatment of disorders of the digestive system and its related research progresses. In clinical practice,Neiguan (PC 6) is usually selected as one of the main acupoints to treat gastrointestinal reactions induced by nausea and vomiting,hiccup, gastrointestinal dyskinesia, gastritis, and intra-gastric examination, cesarean section, etc. Concerning experimental studies, the effects of Neiguan (PC 6), etc. on gastric electrical activities, gastrointestinal motion, gastrointestinal hormone secretion, and gastric rnucesal structure were observed. Resuits indicated that Neiguan (PC 6) is an important and effective acupoint for treatment of gastrointestinal disorders in clinic. Stimulation of Neiguan (PC 6) induced favorable regulation of both the peripheral nerveus system and central nervous system, and changes of the gastrointestinal hormone secretion may contribute to its effects in treating various disorders.