田基黄抗缺氧活性成分的分离鉴定与活性测定

目的分离鉴定田基黄(Herba Hypericci japonici)中抗缺氧活性成分,期望得到具有抗缺氧活性的纯化合物。方法采用多种柱色谱法进行分离纯化,根据理化性质及IR、NMR、ESI-MS等波谱数据进行结构鉴定;对分离得到的化合物采用PC12细胞进行活性追踪,评价其体外抗缺氧活性。结果分离得到5个化合物,分别为3-O-甲基槲皮素(3-O-methylquercetin,1)、原儿茶酸(protocate-chuic acid,2)、二氢山柰酚(dihydronaphthol,3)、二氢槲皮素(dihydroquercetin,4)、绿原酸(chloro-genic acid,5)。结论化合物2~5为首次从田基黄中分离得到;化合物均具有一定的抗缺氧活性,其中化合物2活性最强。     

瓜蒌的抗血小板聚集活性成分研究

目的研究瓜蒌的抗血小板聚集活性成分。方法利用抗血小板聚集的活性追踪方法，采用Sephadex LH-20柱色谱、反复硅胶柱色谱分离，通过IR、^1H—NMR、^13C-NMR、MS等波谱分析手段研究化学结构。结果分离得到7个化合物，分别鉴定为棕榈酸(Ⅰ)，5，5‘-双氧甲基呋喃醛(Ⅱ)，N-苯基苯二甲酰亚胺(Ⅲ)，4-羟基-2-甲氧基苯甲酸(Ⅳ)，4-羟基-烟酸(Ⅴ)，香叶木素-7-O-β-D-葡萄糖苷(Ⅵ)，腺苷(Ⅶ)。结论化合物Ⅳ、Ⅵ、Ⅵ均具有很强的抗血小板聚集作用，化合物Ⅲ为首次从植物中分离得到的天然产物，化合物Ⅴ～Ⅵ为首次从该属植物中分离获得，化合物Ⅱ、Ⅳ为首次从该种植物中分离得到。     

坐骨棘筋膜固定术的临床解剖学研究

Objective The study was to explore the safety, firmness and convenience of the fascia around the ischial spine as a new fixation site for the vaginal fornix. Methods Between June 2007 and January 2008, detailed dissections and related measurements of the regions around the ischial spine were performed on 10 Chinese female cadavers (3 unembalmed and 7 embalmed cadavers). At the same time, the sacrospinous ligament,the fascia on the ischial spine, the iliococcygeus fascia as well as the vaginal fornix were exposed and the pull-out strength sequentially tested using a digital push-pull force gauge. Results The fascia on the ischial spine was firm and strong, with a thickness of 3 about mm. No major vessels or nerves were observed on the ischial spine. The greatest pullout strengths of the sacrospinous ligament,the fascia on the ischial spine, the iliococcygeus fascia as well as the vaginal fornix were (102±26),(64±15),(33±8)and(32±6)N, respectively. Conclusion The fascia at 1 cm from anterior lateral ischial spine, free of major vessels and nerves, is safe and strong and could be used as a new site for suspension in vaginal prolapse.     

坐骨棘筋膜固定术的临床解剖学研究

Objective The study was to explore the safety, firmness and convenience of the fascia around the ischial spine as a new fixation site for the vaginal fornix. Methods Between June 2007 and January 2008, detailed dissections and related measurements of the regions around the ischial spine were performed on 10 Chinese female cadavers (3 unembalmed and 7 embalmed cadavers). At the same time, the sacrospinous ligament,the fascia on the ischial spine, the iliococcygeus fascia as well as the vaginal fornix were exposed and the pull-out strength sequentially tested using a digital push-pull force gauge. Results The fascia on the ischial spine was firm and strong, with a thickness of 3 about mm. No major vessels or nerves were observed on the ischial spine. The greatest pullout strengths of the sacrospinous ligament,the fascia on the ischial spine, the iliococcygeus fascia as well as the vaginal fornix were (102±26),(64±15),(33±8)and(32±6)N, respectively. Conclusion The fascia at 1 cm from anterior lateral ischial spine, free of major vessels and nerves, is safe and strong and could be used as a new site for suspension in vaginal prolapse.     

坐骨棘筋膜固定术的临床解剖学研究

Objective The study was to explore the safety, firmness and convenience of the fascia around the ischial spine as a new fixation site for the vaginal fornix. Methods Between June 2007 and January 2008, detailed dissections and related measurements of the regions around the ischial spine were performed on 10 Chinese female cadavers (3 unembalmed and 7 embalmed cadavers). At the same time, the sacrospinous ligament,the fascia on the ischial spine, the iliococcygeus fascia as well as the vaginal fornix were exposed and the pull-out strength sequentially tested using a digital push-pull force gauge. Results The fascia on the ischial spine was firm and strong, with a thickness of 3 about mm. No major vessels or nerves were observed on the ischial spine. The greatest pullout strengths of the sacrospinous ligament,the fascia on the ischial spine, the iliococcygeus fascia as well as the vaginal fornix were (102±26),(64±15),(33±8)and(32±6)N, respectively. Conclusion The fascia at 1 cm from anterior lateral ischial spine, free of major vessels and nerves, is safe and strong and could be used as a new site for suspension in vaginal prolapse.     

不同制备工艺的瓜蒌薤白提取物药效学比较

目的：对不同工艺制备的瓜蒌薤白提取物（分别简称为水提物WE及醇提物EE）进行药效学比较研究。方法：采用小鼠常压耐心肌缺氧模型,豚鼠离体心脏冠脉流量及体外抗血小板聚集等药效学试验。结果：EE可明显延长小鼠在常压缺氧情况下的存活时间,而相同剂量的WE则无效;相同剂量的EE及WE对离体豚鼠心脏幸免具有明显的扩冠作用,并均可抑制由ADP诱导的血小板聚集。结论：相同剂量的EE的药理作用略优于WE。     

血小板聚集性测定方法主要影响因素的考察

应用比浊法对ADP诱导兔血小板聚集测定方法的主要影响因素、ADP浓度、pH值、溶剂和增溶剂进行了考察,选择出血小板聚集性测定方法的适宜条件。     

醒酒冲剂降低血醇浓度的试验研究

<正> 醒酒灵冲剂具有醒酒解醉的功能。酒醉的主要客观指标是饮酒者体内血液中乙醇浓度的升高。我们以有酒史、健康无病的自愿受试者为试验对象,利用气相色谱法测定醒酒灵冲剂降低血醇浓度的效果。由于人的个体差异很大,故采用平行自身交叉对照试验方法测定。受试者10人,分为二组,第一组先饮一定量的水,半小时后饮酒,然后测不同时间的血中乙醇     

pH值对黄芩甙提取工艺影响的初步考查

本文对碱酸法提取黄芩甙的工艺中四个不同pH值进行了四个不同水平的系统考查,得出较适宜的pH值,即:碱提取为pH6.0;第一次酸沉淀pH1.0;碱溶解pH6.0;第二次酸沉淀pH1.0.并以此制订了新的工艺流程,使黄芩甙纯品收率较原工艺提高了86.35%.     

人参化学成分的研究 10.人参皂甙含量测定方法的比较研究

本文报道了国内外八个人参样品用直接比色法、TLC-比色法、薄层光密度法、大孔树脂—比色法四种分析方法测定人参皂甙的含量,作了比较研究。实验结果表明,四种方法呈现一定的关系,其中薄层—比色法和大孔吸附树脂—比色法结果比较相近,直接比色法含量偏高,薄层光密度法适合分组皂甙及单体皂甙的测定。