1,25(OH)2D3对miR-130b转染的高糖条件下HMC细胞TGF-β1/Smad3及Col-Ⅳ表达的影响

目的 观察高糖环境下1,25(OH)2D3对miR-130b转染肾小球系膜细胞TGF-β1/Smad3、Col-Ⅳ表达水平的变化,探索1,25(OH)2D3是否可通过miR-130b改善糖尿病肾病肾脏纤维化的过程.方法 HMC细胞传代培养,miR-130b mimics 及 miR-130b inhibitor 经 L...     

酵母和植物水解物在中国仓鼠卵巢细胞生产重组蛋白中的应用研究进展

目前，重组蛋白药物主要是由中国仓鼠卵巢(CHO)细胞表达系统生产的。细胞培养技术特别是培养基是影响CHO细胞表达重组蛋白的关键因素。酵母和植物水解物作为细胞培养基的补充物，已广泛应用于无血清培养基的优化，以改善CHO细胞的生产性能，提高重组蛋白药物的质量和产量。本文综述了酵母和植物水解物在CHO细胞生产重组蛋白中的应用，以期为研发更安全、有效的无血清培养基及提高重组蛋白药物的产量提供参考。     

《针灸甲乙经》从脾论治抑郁症学术思想探析

《针灸甲乙经》是现存最早的一部针灸学专著，其从脾论治的学术思想贯穿抑郁症的因机、选穴及刺灸法三个方面。《针灸甲乙经》认为，抑郁症以“脾虚”为基本病机：脾虚土衰，则意伤神疲；土虚木郁，则脏气失达；子病及母，则积思成疾；土不制水，则命火微之、如堕深渊。治疗抑郁症选穴时应注意以脾为要、实土通阳，且宜与脾相应、和调五脏。在选择刺灸法方面，治疗抑郁症多用缪刺法和刺血法，可起到祛瘀生新、助脾统血之功，同时也宜用灸法，以达温阳举陷、扶土升清之效。深入挖掘《针灸甲乙经》从脾论治抑郁症学术思想，对临床诊治抑郁症有积极的指导意义。     

雷帕霉素灌胃对糖尿病大鼠视网膜的保护作用及机制

目的 观察雷帕霉素对糖尿病大鼠视网膜的保护作用，并探讨其作用机制与PI3K/AKT/mTOR信号通路的关系。方法 将50只大鼠随机分为正常对照组10只和糖尿病组40只，糖尿病组采用高脂饮食及腹腔注射链脲佐菌的方式制作糖尿病模型，对照组给予普通饲料喂养。取造模成功的30只大鼠，随机分为模型对照组、阳性对照组和雷帕霉素组各10只，阳性对照组给予二甲双胍20 mg/（kg·d）灌胃，雷帕霉素组给予雷帕霉素1 mg/（kg·d）灌胃，模型对照组给予等体积纯净水灌胃。4周后大鼠取血检测空腹血糖；取视网膜组织，行HE染色后观察视网膜神经细胞数及视网膜病理组织形态变化；Western blotting法检测视网膜组织中PI3K、AKT、mTOR蛋白表达。结果 与正常对照组比较，模型对照组、阳性对照组及雷帕霉素组空腹血糖均升高（P均<0.05）；与模型对照组比较，阳性对照组和雷帕霉素组空腹血糖均降低（P均<0.05）。正常对照组视网膜形态无明显病理改变；与正常对照组比较，模型对照组视网膜形态改变，细胞水肿严重，细胞膜结构紊乱，视网膜厚度明显变薄，神经节细胞数量减少；与模型对照组比较，阳性对...     

线粒体DNA突变与氨基糖甙类抗生素致聋研究进展

线粒体脱氧核糖核酸（mitochondrial deoxyroibose nucleic acid，mtDNA）是唯一存在于核外的遗传物质，具有独特的遗传特性，如严格的母系遗传、具有半自主性、不与组蛋白结合，修复能力低下，容易发生碱基突变。氨基糖甙类抗生素耳聋具有家族聚集的现象，遗传的线粒体突变是氨基糖甙类抗生素致聋（aminoglycoside antibiotics induced deafness，AAID）等非综合征耳聋的原因之一。     

Mechanism of Polygonum capitatum in treatment of Helicobacter Pylori associated gastritis by network pharmacology,microarray data analysis and molecular docking北大核心CSCD

Aim To explore the mechanism of Polygonum capitatum(PC)in the treatment of Helicobacter Pylori associated gastritis(HAG). Methods The databases were used to identify the target of PC active compounds and HAG-related genes,and the intersection was taken to obtain the potential targets of PC treatment of HAG. The interaction network diagram of “drug-active compound-target-disease” and the protein-protein interaction(PPI)network of potential target protein interaction in HAG treated by PC were constructed by software Cytoscape 3.6.0. The important nodes in the network were screened by several topological indexes,and the GO and KEGG enrichment were analyzed by STRING database to obtain the potential signaling pathway of PC in the treatment of HAG. The binding ability of PC active components with key target proteins was observed by molecular docking method. On this basis,the related targets of PC in the treatment of HAG were verified in vivo and in vitro experiments. Results The PC active compounds and targets were identified through the database,and the “drug-active compound-target-disease” network diagram and the PPI network of potential target proteins were constructed. Combined with several topological indexes,the PPI network of potential target-protein interaction was analyzed,and 52 hub genes were screened. Further bioinformatics analysis and high-throughput sequencing revealed that PC exerted an effect on HAG through the Akt/NF-κB/NLRP3 pathway. Based on this,it was found that PC could reduce IL-18 and IL-1β in HAG GES-1 cells and HAG SD rats,up-regulate Akt and its phosphorylation level and reduce NF-κB expression,inhibit the activation of NLRP3 inflammatory body,so as to improve HAG inflammatory response. Conclusions PC could exert a therapeutic effect on HAG by activating Akt and its phosphorylation level,and inhibiting the expression of NF-κB and NLRP3 inflammasome related factors. This study provides a theoretical basis for explaining the mechanism of PC in the treatment of HAG. © 2023 Publication Centre of Anhui Medical University. All rights reserved.     

大果木姜子精油及浸膏对心肌损伤大鼠的保护作用

目的 探讨大果木姜子精油、浸膏对心肌损伤大鼠的保护作用。方法 将SD大鼠随机分为正常对照组(CG)、模型组(MG)、复方丹参组(FFDS)、尼可地尔组(Nic)，大果木姜子精油低剂量组(GO-1)、精油高剂量组(GO-2)，浸膏低剂量组(GE-1)、浸膏高剂量组(GE-2)，每组11只。除CG外，各组大鼠冰水力竭游泳14 d，第12天后于颈部皮下多点注射异丙肾上腺素(sisoprotereno,ISO)50 mg/kg，连续3 d，建立心肌缺血损伤模型。造模后灌胃相应药物，每天2次，连续7 d。采集大鼠治疗前后Ⅱ导联心电图，观察治疗后HE、Masson着染的腹主动脉、心肌组织病理变化，试剂盒检测心肌标志物门冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)、肌酸激酶同工酶(CK-MB)、肌钙蛋白Ⅰ(cTn-Ⅰ)，炎性因子白细胞介素-6(IL-6)、肿瘤坏死因子α(TNF-α)，和超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量。结果 造模大鼠Ⅱ导联心电图表现主要为室上性心律失常，心肌组织出现炎性细胞浸润，细胞间质发生胶质沉积，说明大鼠心肌损伤模型构建成功。与CG相比，MG大鼠心电ST段...