呼吸道合胞病毒和人类偏肺病毒致儿童急性毛细支气管炎临床分析

目的：探讨呼吸道合胞病毒（RSV）和人类偏肺病毒（hMPV）所致婴幼儿急性毛细支气管炎的临床表现、住院时间、肺功能的差异。方法：收集苏州大学附属儿童医院2011-2012年收治住院的急性呼吸道感染患儿,无菌负压吸引法吸取新鲜痰液1~2 mL,采用直接免疫荧光法（DFA）检测常见病毒抗原,逆转录-聚合酶链反应（RT-PCR）扩增hMPV的N基因,以明确诊断。选取其中RSV急性毛细支气管炎155例（RSV组）、hMPV急性毛细支气管炎46例（hMPV组）进行临床分析,对部分RSV（50例）、hMPV（31例）感染患儿进行肺功能检测,并选取同龄健康儿童49例作为正常对照组。结果：确诊为儿童急性毛细支气管炎的住院患儿共计653例,其中RSV感染占23.74%（155/653）,hMPV感染占7.04%（46/653）,二者主要临床表现均为低氧血症、发热、咳嗽、喘息、鼻塞流涕、气促,但hMPV组低氧血症、发热、喘息、气促的发生率比RSV组低,住院时间比RSV组短（P均<0.05）。RSV和hMPV急性毛细支气管炎患儿肺功能均有不同程度受损,主要表现为小气道功能障碍,但两组肺功能各指标比较差异均无统计学意义（P均>0.05）。结论：hMPV和RSV一样是儿童急性毛细支气管炎常见病原之一。RSV、hMPV急性毛细支气管炎患儿临床表现、肺功能相似,但后者症状较轻、住院时间较短。潮气呼吸肺功能检测有利于评估RSV、hMPV感染后肺功能损害程度,可为临床诊治提供客观依据。     

Effect of structural domain Ⅱ of HCV 5' noncoding region on its translation initiation activity

Objective To examine the role of domain Ⅱ of hepatitis C virus(HCV) 5'noncoding region (5'NCR) in its translation initiation activity. Methods The fragment of HCV 5' NCR with deletions of 5'-proximal 118 nucleotides were amplified with PCR, then was used to substitute for the full length HCV 5'NCR of plasmid pCMVNCRIuc, a firefly luciferase(Flue) eukaryotic expression plasmid regulated by HCV 5' NCR, to generate recombinant plasmid pCN1-d3, pCMVNCRIuc, pCN1-d2 (modified pCMVNCRluc with deletions of HCV 5' NCR nt1-43) and pCN1-d3 were transfected into HepG2 cells with liposome transfection protocol, respectively, and the relative luciferase activity was measured to analyze the regulatory effect of the truncated 5'NCR on Fluc gene expression. Meanwhile the Fluc mRNA levels were detected by RT-PCR. Results The recombinant plasmid was successfully constructed. The Fluc mRNA levels of the 3 plasmids were not significantly different(P 0.05), and there were also no significant difference between the relative luciferase activity of plasmids pCN1-d2 and pCMVNCRluc(P 0.05). However, that of pCN1-d3 was decreased significantly(P < 0.01, compared with pCN1-d2 or pCMVNCRluc). Conclusion Structural domain Ⅱ of HCV 5' NCR plays an important role in itstranslation initiation activity.     

芍药甘草汤抗胃溃疡作用的谱效关系

目的 研究芍药甘草汤抗胃溃疡作用的谱效关系.方法 制备11批芍药甘草汤,以无水乙醇造模法制备胃溃疡大鼠模型,以胃康灵为阳性对照,考察各批芍药甘草汤对模型大鼠胃组织中防御因子一氧化氮(NO)、表皮生长因子(EGF)、超氧化物歧化酶(SOD)和攻击因子丙二醛(MDA)含量的影响.采用《中药色谱指纹图谱相似度评价系统(200...     

Fribrinolysis Kinetics and Its Application

A new, convenient, and rapid method for kinetic measurement of human fibrinolysis was established. The alteration of absorbance (A) in the process of blood coagulation and lyses was auto-matically scanned and recorded using a UV2000 spectrophotometer connected to a computer. The parameters of human fibrinolysis kinetics were established. Urokinase at 20 U/mL was the optimal concentration used. There was significant difference in fibrinolysis kinetics and plasma plasminogen concentration between 22 normal subjects and 27 patients with acute myeloblastic leukemia (P<0.05 and <0.01 respectively). The coefficience of variation was (5.24±1.51)%. This method could also be used to measure the plasma fibrinogen concentration at the same time. It was concluded that this method was stable and was capable of providing dynamic, direct experimental data and multipareme-ters for clinicians. It was also valuable in evaluating the anti- and pro-fibrinolytic capcity of patients' plasmas, allowing for monitoring of therapy, choice of drugs and adjustment of drug concentrations.     

Effect of structural domain Ⅱ of HCV 5' noncoding region on its translation initiation activity

Objective To examine the role of domain Ⅱ of hepatitis C virus(HCV) 5'noncoding region (5'NCR) in its translation initiation activity. Methods The fragment of HCV 5' NCR with deletions of 5'-proximal 118 nucleotides were amplified with PCR, then was used to substitute for the full length HCV 5'NCR of plasmid pCMVNCRIuc, a firefly luciferase(Flue) eukaryotic expression plasmid regulated by HCV 5' NCR, to generate recombinant plasmid pCN1-d3, pCMVNCRIuc, pCN1-d2 (modified pCMVNCRluc with deletions of HCV 5' NCR nt1-43) and pCN1-d3 were transfected into HepG2 cells with liposome transfection protocol, respectively, and the relative luciferase activity was measured to analyze the regulatory effect of the truncated 5'NCR on Fluc gene expression. Meanwhile the Fluc mRNA levels were detected by RT-PCR. Results The recombinant plasmid was successfully constructed. The Fluc mRNA levels of the 3 plasmids were not significantly different(P 0.05), and there were also no significant difference between the relative luciferase activity of plasmids pCN1-d2 and pCMVNCRluc(P 0.05). However, that of pCN1-d3 was decreased significantly(P < 0.01, compared with pCN1-d2 or pCMVNCRluc). Conclusion Structural domain Ⅱ of HCV 5' NCR plays an important role in itstranslation initiation activity.     

军事训练致16例腕舟状骨骨折

2003年1月～2005年10月我院共收治军事训练中致腕舟骨骨折病人16例,通过石膏固定或手术植骨内固定,疗效满意.现报告如下.     

内毒素增加微血管通透性的细胞信号机制

内毒素(LPS)主要激活PMNs和内皮细胞,通过复杂的信号转导通路,引起微血管内皮细胞屏障功能和形态的损伤,使血管通透性增加,血容量下降,是导致内毒素性休克和多器官功能损害的重要原因.     

Effect of structural domain Ⅱ of HCV 5' noncoding region on its translation initiation activity

Objective To examine the role of domain Ⅱ of hepatitis C virus(HCV) 5'noncoding region (5'NCR) in its translation initiation activity. Methods The fragment of HCV 5' NCR with deletions of 5'-proximal 118 nucleotides were amplified with PCR, then was used to substitute for the full length HCV 5'NCR of plasmid pCMVNCRIuc, a firefly luciferase(Flue) eukaryotic expression plasmid regulated by HCV 5' NCR, to generate recombinant plasmid pCN1-d3, pCMVNCRIuc, pCN1-d2 (modified pCMVNCRluc with deletions of HCV 5' NCR nt1-43) and pCN1-d3 were transfected into HepG2 cells with liposome transfection protocol, respectively, and the relative luciferase activity was measured to analyze the regulatory effect of the truncated 5'NCR on Fluc gene expression. Meanwhile the Fluc mRNA levels were detected by RT-PCR. Results The recombinant plasmid was successfully constructed. The Fluc mRNA levels of the 3 plasmids were not significantly different(P 0.05), and there were also no significant difference between the relative luciferase activity of plasmids pCN1-d2 and pCMVNCRluc(P 0.05). However, that of pCN1-d3 was decreased significantly(P < 0.01, compared with pCN1-d2 or pCMVNCRluc). Conclusion Structural domain Ⅱ of HCV 5' NCR plays an important role in itstranslation initiation activity.     

磁性支具外固定治疗四肢训练伤152例

2003年2月～2006年4月,我们采用自行研制的磁性支具外固定治疗四肢训练伤152例,疗效满意。现报告如下。