CT对乳腺癌化疗后所致脂肪肝的诊断价值

目的探讨CT对化疗性脂肪肝的诊断及监测价值。方法 68例患者接受乳腺癌根治术或改良根治术,并行术后常规化疗,根据患者的血脂水平及肝脏CT检查确诊为脂肪肝。结果化疗3、6周期后和化疗后0.5、1年分别有54、65和53、9例病人提示为脂肪肝。结论化疗药物可以导致肝脏的脂肪变性,发展为脂肪肝。临床医生应意识到脂肪肝的危害性,早诊断,早处理。     

Enhanced CD3~+/CD4~+ T Cell Activities and Modulation of Th1/Th2 Lineage Development in Irradiated Rats Due to Treatment with the Male Zooid of Antheraea Pernyi Extracts

Objective:Cancer patients undergoing large dose radiotherapy exhibit multifaceted defects in their immune capacity that are likely to contribute to an increased susceptibility to infections and disease progression.The immune impairment may also constitute a barrier to effective immunotherapeutic interventions.Here,we evaluate whether supplementation with the male zooid of Antheraea pernyi extracts could enhance immune function in irradiated rats.Methods:Fifty male Wistar rats were randomly divided into a...     

胰腺癌组织中淋巴管生成的机制及意义

目的：探讨胰腺癌组织中血管内皮生长因子C（vascular endothelial growth factor-C,VEGF-C）、血管内皮生长因子D（vascular endothelial growth factor-D,VEGF-D）与微淋巴管密度（microvessel vessd density,MLVD）、微血管密度（microvessel density,MVD）及淋巴结转移之间的相关关系,阐明癌周淋巴管增生的机制及意义.方法：免疫组化检测30例胰腺癌组织中VEGF-C、VEGF- D、血管内皮生长因子受体3（vascular endothelial growth factor receptor-3, VEGFR-3）（MLVD）以及CD34（MVD）蛋白的表达,RT-PCR检测VEGF-A、VEGF-C、VEGF-D mRNA在30例胰腺癌新鲜组织中的表达.结果： VEGF-C、VEGF-D蛋白阳性率分别为73%（22/30）和57%（17/30）,肿瘤周边部位显著高于肿瘤中心部位,差异有统计学意义,P ＜0.05; 其表达与肿瘤的部位、分化程度以及组织学类型无关,而与肿瘤的TNM分期有关,Ⅲ、Ⅳ期显著高于Ⅰ、Ⅱ期.在VEGF-C蛋白阳性组,MVD高于阴性组, P=0.047 2,MLVD亦高于阴性组,P＜0.01,淋巴结转移增多,P=0.031 8;VEGF- D蛋白阳性组与阴性组相比MVD无变化, P=0.07,MLVD高于阴性组, P＜0.01,淋巴结转移增加,P=0.017 9.结论： VEGF-C参与胰腺癌血管生成和淋巴管生成的调控,VEGF-D只参与淋巴管生成的调控.VEGF-C和VEGF-D诱导胰腺癌淋巴管生成,促进肿瘤细胞淋巴道转移.     

麻黄属6种植物茎叶表皮的扫描电镜观察

目的：通过对麻黄属植物茎、叶表面气孔及微形态特征观察,为其遗传育种及分类研究提供重要微观特征和依据。方法：利用扫描电镜观察6种麻黄属植物茎、叶表面气孔密度、气孔长轴、短轴、气孔形态及茎、叶表面特征。结果：6种麻黄属植物之间气孔密度、茎、叶表面特征存在不同程度的差异；气孔表现出典型旱生植物的特征,略下陷,气孔保卫细胞被厚的角质层覆盖,并形成突起状角质唇状物；气孔大小、形状在种之间无明显区别；叶表面、茎表面气孔均属于无规则型,但形状明显不同,前者主要呈长方形或六边形,后者为窄条形或哑铃形。结论：麻黄属植物气孔的类型及特征比较固定和保守,气孔的大小、形状仅在种内有变异,种间差异较小,分种的价值不大,但在植物分类上具有重要的鉴定价值。     

出血性脑梗死52例临床分析

[目的]探讨出血性脑梗死（HI）的发病率、病因、临床和影像学特点以及短期预后。[方法]对2001年1月~2005年12月收治的52例HI患者的临床资料进行回顾性分析。[结果]HI的发生率为7.3%。继发出血多发生在缺血性卒中后2周内,第1周为31例（59.6%）,第2周为17例（32.7%）。发病1周内发生HI者的短期预后较1周后发生者差（P〈0.05）。根据影像学特点,HI可分为血肿型和非血肿型。与非血肿型相比,血肿型的短期预后明显不良（P〈0.05）。HI的死亡率为15.4%。[结论]对于心源性脑栓塞和大面积脑梗死病例在发病1-2周内进行影像学复查,将有助于HI的及时诊断和治疗。     

Enhanced CD3+/CD4+ T cell activities and modulation of Th1/Th2 lineage development in irradiated rats due to treatment with the male zooid of antheraea pernyi extracts

Objective  Cancer patients undergoing large dose radiotherapy exhibit multifaceted defects in their immune capacity that are likely to contribute to an increased susceptibility to infections and disease progression. The immune impairment may also constitute a barrier to effective immunotherapeutic interventions. Here, we evaluate whether supplementation with the male zooid of Antheraea pernyi extracts could enhance immune function in irradiated rats. Methods  Fifty male Wistar rats were randomly divided into a control group, a simple radiation group and a treatment group. The mice in the simple irradiation and treatment groups were given whole-chest irradiation with 6Gy. In the treatment group, the male zooid of Antheraea pernyi extracts was gavaged at the doses of 16.53mg/kg (large dose group), 2.62mg/kg (medium dose group), and 0.564mg/kg (small dose group) once a day for 14 days. The thymus and spleen indices were calculated. T cell subsets in peripheral blood were determined by flow cytometry and the expressions of IL-2, IFN-γ, IL-4 and IL-10 in sera were determined by ELISA on the 15th day. Results  The thymus index and spleen index of the high dose treatment group were statistically lower than that of the control group and higher than that of the radiation group (P<0.01). CD3+ and CD4+ T cells in the peripheral blood were increased in the high dose treatment group and decreased in the radiation group (P<0.01). Expression of IL-2 and INF-γ in the radiation group was lower than that in control, and significantly increased during therapy. The production of IL-4 and IL-10 could be induced by radiation and was inhibited in the high dose treatment group (P<0.01). Conclusion  Our data indicate that the male zooid of Antheraea pernyi extracts may be administrated to improve immune function in irradiated rats and reverse the radial immune inhibition of rats by stimulating the proliferation of Th cells and inducing the differentiation of Th2 to Th1. This work was supported by the grants from the National Natural Science foundation of China (No. 30472260), the Administration of Traditional Chinese Medicine of Shandong Province, China(No. 2005-109),, and the Health Protection Committee of Shandong Province, China(No. 2006059).     

Syk和VEGF-C蛋白在胰腺癌中的表达及其与淋巴转移的关系

目的探讨胰腺癌组织中Syk和VEGF—C蛋白的表达及其在淋巴转移中的临床意义。方法应用免疫组化技术检测40例胰腺癌组织中Syk和VEGF—C蛋白的表达情况。结果正常胰腺组织表达Syk蛋白，在慢性胰腺炎中有中等表达，其阳性率分别为100．0％和77．8％；在胰腺癌中阳性率为27．5％，大多数呈中等或弱阳性表达，与前两者比较有显著性差异（P=0．0002，P=0．0079）；Syk蛋白与肿瘤的发生部位、分化程度和有无淋巴结转移间无显著性差异；与TNM分期有关，Ⅰ～Ⅱ期组表达明显高于Ⅲ～Ⅳ期组（P=0．0245）。胰腺癌组织中VEGF—C蛋白阳性率为72．5％（29／40），肿瘤周边部位显著高于肿瘤中心部位，具有统计学意义。在VEGF—C蛋白阳性组，淋巴结转移（P=0．0398）增多。Syk和VEGF—C具有关联性。结论Syk蛋白在胰腺癌中表达缺失，并与胰腺癌的发展有关；VEGF—C参与胰腺癌血管生成和淋巴管生成的调控，促进肿瘤细胞淋巴道转移；两者的表达具有相关性。     

携带人α1,2-岩藻苷转移酶基因转基因小鼠的建立

目的：建立携带人α1，2-岩藻苷转移酶[alpha（1，2）-fucosyltransferase，HT]基因的转基因小鼠模型。方法：采用受精卯显微注射法，将人HT转基因构件导入昆明白小鼠受精卵的雄原核内，然后将注射后仍健康的受精卵植入假孕母管内，等其自然分娩。对G0代小鼠，应用聚合酶链反应（PCR）和Southern杂交检测人HT基因的整合情况、应用逆转录PCR（RT—PCR）检测人HT基因mRNA水平的表达，应用流式细胞计数（FCM）检测H抗原及α—Gal抗原在小鼠外周血单核细胞（PBMCs）表面的表达。结果：注射后卵的存活率和幼鼠出生率分别为84．9％（968／1140）和10．8％（105／968），外源基因的整合率为7．6％（8／105），基因整合阳性小鼠中有7只的心、肝、肾及骨骼肌组织均有人HT mRNA表达，并且PBMCs表面H抗原表达阳性，表达强度为人PBMCs的95％-150％，同时α—Gal抗原表达减少，为正常小鼠的5％-10％。转基因小鼠已经稳定传3代。结论：携带人α1，2-岩藻糖苷转移酶基因的转基因小鼠模型已制备成功。     

高效液相色谱法测定蒲地蓝消炎片中黄芩苷的含量

目的建立高效液相色谱法测定蒲地蓝消炎片中黄芩苷含量的方法.方法采用Zorbax ODS C18(250 mm×4.6 mm,5μm)色谱柱,以甲醇-水-磷酸(47∶53∶0.2)为流动相,流速1.0 mL·min-1,检测波长为280nm,用回流法提取蒲地蓝消炎片中黄芩苷.结果黄芩苷在0.1265～0.6325 mg·L-1(r=0.999 7,n=5)范围内线性关系良好,平均回收率为97.24%.结论本方法操作简便、精密度好,适用于蒲地蓝消炎片的质量控制.     

FasL表达在肝癌肿瘤免疫逃避中的意义

目的 :探讨肝癌中FasL表达在肿瘤免疫逃避中的意义。方法 :采用免疫组织化学法和脱氧核糖核酸末端转移酶介导的缺口末端标记 (TUNEL)技术 ,对 4 1例肝癌组织中FasL表达及其肿瘤浸润淋巴细胞凋亡的关系进行研究。结果 :肝癌FasL表达阳性率为 4 1 4 6% ,FasL阳性组肝癌肿瘤浸润淋巴细胞 (tumorinfiltratinglymphocytes ,TIL )凋亡指数约是FasL阴性组肝癌的 2倍 ,两组差异有显著意义。结论 :肝癌FasL表达在肿瘤免疫逃避中有重要意义