IL-36γ、p38MAPK通路和Th17细胞因子在扁平苔藓中的表达

目的:检测IL-36及p38丝裂原活化蛋白激酶(p38MAPK)通路和Th17细胞因子在扁平苔藓中的表达。方法:应用Envision免疫组化法检测58例扁平苔藓及19名正常皮肤标本中IL-36γ、磷酸化-p38MAPK(p-p38)和IL-17A的表达。结果:IL-36/、p-p38和IL-17A在扁平苔藓皮损中的蛋白表达阳性率为86.21%、79.31%和94.83%,显著高于正常对照标本阳性率为68.42%、15.79%和63.16%(均P0.01)。扁平苔藓组中,IL-36γ与p-p38、IL-36/与IL-17A的表达水平存在显著正相关(P0.001),两因子表达强度有显著差异(P0.05)。结论:p38MAPK通路和Th17细胞因子可能与扁平苔藓的发病有关。     

白藜芦醇抗恶性黑素瘤血管新生的体外研究

目的:检测白藜芦醇抗恶性黑素瘤血管形成的影响.方法:体外小管形成实验检测白藜芦醇对人脐静脉内皮细胞(HUVEC)体外小管形成的影响,蛋白印迹法检测白藜芦醇对A375细胞血管内皮细胞生长因子(VEGF)及环氧合酶-2(COX-2)表达的影响.结果:白藜芦醇能明显抑制HU-VEC管状结构的形成;100μmol/L白藜芦醇处理后,二维基质胶中HUVEC形成不完整的,可见松散的管状结构,小管的直径及面积均明显低于对照组(P＜0.05).白藜芦醇明显下调A375细胞VEGF及COX-2的表达,且呈量效及时效关系.结论:白藜芦醇可能通过调节VEGF及COX-2的表达进而抑制恶性黑素瘤血管新生,这可能是其对黑素瘤抗癌效应的机制之一.     

年龄、性别及肥胖等因素对人体内脂肪分布的影响

人体内脂肪过多可引起多种合并症而使生存率下降。近年研究,脂肪蓄积程度与患病未必呈直线关系,腹部肥胖等脂肪分布异常的代谢性疾病(糖尿病、高脂血症及高尿酸血症等)与高血压、缺血性心脏病均有密切关系,脂肪分布有男女性别之差别并随年龄增长而变化。肥胖的脂肪分布可用腰围(W)/臀围(H)表示,W/H比例增大,说明上身比下身肥胖并易发生代谢异常。作者用CT扫     

Association of HLA-DRB1，DQB1 Alleles with Chronic Urticaria

In order to investigate the association of genotypes of HLA DRB1 and HLA-DQB1 alleles with the genetic susceptibility of chronic urticaria (CU), genotypes of HLA-DRB1 and HLADQB1 genes were detected by polymerase chain reactions with sequence-specific primers (PCR-SSP) in 42 patients with CU (19 men and 23 women, mean age 30.67~12.45 y old as well as 193 racially matched healthy persons in ethnic Han from Hubei provinece. Gene frequencies of HLA-DRB1 *12, *0901(RR=3.11,X^2=7.579, P=0.006； RR=2.47,X^2=5.684, P=0.017) were signifi-cantly in creased in CU patients as compared with that in healthy people. Gene frequencies of HLADQB1 *05 (RR=0.26, X^2=6.683, P=0.01) were significantly decreased in CU patients. It was suggested that CU was found strongly associated with HLA-DRB1 *12, *0901 and HLA-DQB1 *05, the former might be the genetic markers for susceptibility to CU, but the latter might play a resistive role。     

Establishment of Mus Skin Photo-damage Model by 8-MOP plus UVA Irradiation

To establish a simple and reliable animal model of skin photo-damage, 20 mice were treated with 8-MOP and exposed to UVA (UVA 320–400 nm) for 24 h. After irradiation, the structure of the epidermis and dermis, collagen fibers, elastic fibers were observed by using HE staining and Weigert technique and compared with the normal controls. The acanthosis and epidemis proliferation with accompanying hyperkeratosis and parakeratosis were observed. Inflammatory infiltration was noted in the dermis. The elastic fibers became coarse, irregularly arranged and clustered, with their number increased. The collagen fibers showed obvious degeneration and some amorphous materials could also be observed. The blood vessels were irregularly dilated and vascular walls were thickened, with infiltration of inflammatory cells. It is concluded that murine photodamage model can be quickly, conveniently and reliably established by means of 8-MOP/UVA.     

MCM5蛋白在银屑病、鲍温病和鳞状细胞癌中的表达

目的 研究微小染色体维持蛋白 5(MCM5) 在银屑病、鲍温病和鳞状细胞癌中的表达情况,并与上述疾病中细胞增殖核抗原(PCNA)蛋白的表达相对比,以寻找一种新的反映皮肤细胞增殖活性的标志物.方法 采用免疫组化ABC 法检测 MCM5 蛋白和 PCNA蛋白在 16 例寻常性银屑病、16 例鲍温病、38 例鳞状细胞癌和 12 例正常人皮肤组织石蜡包埋切片中的表达,并计算其标记指数.结果 ①银屑病组、鲍温病组和鳞状细胞癌组中 MCM5 蛋白标记指数明显高于正常对照组(P<0.01).②各研究组中,MCM5 和 PCNA 蛋白免疫组化染色特点相似.结论 MCM5 蛋白是检测皮肤组织中细胞增殖活性的良好标志物.     

不同抗真菌药治疗白念珠菌性阴道炎模型小鼠的疗效比较

目的 比较特比萘芬、氟康唑、伊曲康唑等抗真菌药治疗小鼠白念珠菌性阴道炎的疗效。方法构建ICR小鼠白念珠菌性阴道炎模型，应用特比萘芬、氟康唑、伊曲康唑灌服治疗，并在灌药后不同时间动态观察阴道灌洗液真菌载量。结果 特比萘芬组在不同时间点其小鼠阴道灌洗液真菌载量明显高于氟康唑组及伊曲康唑组（P＜0.01），连续灌药1周后其真菌载量仍处于较高水平。氟康唑组与伊曲康唑组从灌服药物后第2天开始其阴道灌洗液真菌载量明显减少，灌服药物后第3天开始阴道灌洗液真菌载量即处于极低水平，表明两组疗效显著，但两者之间疗效差异无显著性（P＞0.05）。结论 特比萘芬治疗小鼠白念珠菌性阴道炎的疗效欠佳，而氟康唑与伊曲康唑治疗小鼠白念珠菌性阴道炎疗效显著。     

内皮素3上调黑素瘤细胞株A375表达骨桥蛋白的研究

目的 探讨内皮素受体(ETR)-B拮抗剂BQ788和磷脂酰肌醇-3激酶(PI3K)抑制剂LY294002对黑素瘤细胞株A375骨桥蛋白基因的表达。方法 对照组在人A375细胞培养体系中加入100 nmol/L内皮素3,试验组除加入100nmol/L内皮素3外,分别加入1μmol/L BQ788和20μmol/L、50μmol/L LY294002,采用RT-PCR和蛋白质印迹方法检测骨桥蛋白的mRNA和蛋白表达。结果 ETR-B拮抗剂BQ788和PI3K抑制剂LY294002均能显著减少人恶性黑素瘤细胞株A375中骨桥蛋白mRNA和蛋白的表达,与对照组比较,差异均有统计学意义(P<0.05)。高浓度较低浓度LY294002对骨桥蛋白表达的抑制作用更明显(P<0.05)。结论 内皮素3/ETR-B能通过激活PI3K信号通路上调骨桥蛋白的表达。