喜炎平注射液治疗小儿手足口病的临床疗效

目的:分析喜炎平注射液治疗小儿手足口病的临床疗效。方法:选取小儿手足口病患者98例作为研究对象,按治疗方法的不同,分为对照组(n=49例)和观察组(n=49例)。对照组给予利巴韦林治疗,观察组在对照组基础上采取喜炎平注射液治疗,观察对比两组患者的临床效果。结果:观察组的总有效率(95.92%)显著高于对照组的(81.63%),差异有统计学意义(P<0.05);观察组的皮疹消退时间、愈合时间分别为(3.45±1.24)d、(7.54±1.39)d,优于对照组的(5.65±2.34)d、(12.43±2.78)d,差异均有统计学意义(P<0.05);两组患者均无严重不良反应发生。结论:小儿手足口病采取喜炎平注射液治疗的临床效果显著,改善患儿的临床症状,值得临床推广应用。     

阿德福韦酯治疗乙型肝炎的临床效果及其不良反应

目的：分析阿德福韦酯治疗乙型肝炎的临床效果及其不良反应。方法选取慢性乙型肝炎患者110例作为研究对象,均给予阿德福韦酯治疗,根据药物剂量的不同,分为低剂量组（38例,剂量为3 mg/d）、中剂量组（38例,剂量为9 mg/d）及高剂量组（34例,剂量为12 mg/d）,治疗3个月后,观察对比3组患者的临床疗效及其不良反应。结果低剂量组、中剂量组、高剂量组的阳性率分别为39.47%、68.42%、73.53%,差异有统计学意义（P<0.05）;低剂量组血清肌酐水平为（62.34±5.64）mmol/L,中剂量组血清肌酐水平为（63.48±6.21）mmol/L,高剂量组血清肌酐水平为（96.52±12.32）mmol/L,差异有统计学意义（P<0.05）;低剂量组血清磷水平为（4.45±1.23）mg/L,中剂量组血清磷水平为（5.48±1.38）mg/L,高剂量组血清磷水平为（6.32±2.03）mg/L,差异有统计学意义（P<0.05）。结论应用阿德福韦酯治疗乙型肝炎的剂量不宜超过10 mg/d,高剂量阿德福韦酯治疗产生的不良反应较大,但低剂量也会影响其临床效果,因此临床上应选择合适的阿德福韦酯剂量治疗乙型肝炎。     

hSav1蛋白高表达对Mst1介导的细胞凋亡的影响

Objective To elucidate the effect of hSavl expression on Mstl-mediated apoptosis in HeLa cells. Methods Plasmids pCMV-HA-hSav1 and pcDNA/4TO-Flag-Mst1 were constructed and cotransfected into HeLa cells. Triple immunofluorescent labeling of hSav1, Mst1 and nucleus was performed to determine their subcellular localization. Plasmids pCMV-HA-hSav1 and/or pcDNA/4TO-Flag-Mst1 were transfected into HeLa cells, and 36 hours later cisplatin (50 μmol/L) as a pro-apoptotic agent was added for 14 hours. Cell apoptosis was analyzed by annexin V/PI assay. Results Plasmids pCMV-HA-hSav1 and pcDNA/4TO-Flag-Mst1 were constructed and the authenticity of constructs was verified by sequencing. The binding in vitro showed that hSavl could be detect from the anti-Mstl immunoprecipitation complex. The immunofluorescent labeling showed that hSavl and Mstl had the same localization in cells. Overexpressed protein hSavl did not induce a significant cell apoptosis. However, co-expression of hSavl with Mstl resulted in a significant increase of apoptosis above the level seen with Mstl alone (24. 5% ± 2.4% vs. 39.3% ± 4.0%, P < 0. 05). Conclusion Our findings indicate that hSavl is a newly identified protein that interacts with Mst1 and a, augments Mst1-mediated apoptosis.     

程序性死亡分子1免疫治疗在B细胞淋巴瘤中的研究进展

免疫检查点阻滞剂抗程序性死亡分子1（PD-1）／PD-1配体（PD-L1）抗体类药物的研发是目前肿瘤治疗领域的研究热点。越来越多的临床试验证实抗PD-1／PD—L1抗体在各类肿瘤的治疗中具有良好的疗效和安全性。研究已证实该类药物在实体瘤中具有显著疗效,其在淋巴瘤治疗中的独特机制也逐渐被发现,文章就PD-1在B细胞淋巴瘤中的最新研究进展进行介绍。     

盐酸氮卓斯汀滴眼液治疗儿童春季结膜炎的临床观察

目的评价盐酸氮卓斯汀滴眼液对儿童春季角结膜炎的治疗效果。方法入选儿童春季结膜炎62例（124眼）,给予患者0.05%盐酸氮卓斯汀滴眼液,2次/d,早晚各1次,每眼1滴。症状严重者,剂量可增加至4次/d。在症状消失后,仍应持续滴用本品滴眼液1周。采用自身对照方法观察治疗前后的春季角结膜炎症状和体征的变化及副作用。结果随诊2-18个月,所有患者在用药1周后症状和体征均有不同程度改善。盐酸氮卓斯汀滴眼液治疗儿童春季结膜炎的总有效率为96.7%,患者的各项症状和体征均得到显著的改善。治疗期间未观察到明显的不良反应。结论盐酸氮卓斯汀滴眼液治疗儿童春季结膜炎具有较好的疗效和安全性。     

肝衰竭中血清转化生长因子-β1的检测及临床意义

目的：探讨血清转化生长因子β1（TGF-β1）对肝衰竭（LF）预后判断的意义。方法：选择LF患者135例,测定血清TGF-β1分析其水平变化与肝脏损伤程度的关系。结果：LF患者血清TGF-β1明显高于健康组,随着肝病基础之肝硬化、肝癌发展,TGF—β1水平逐渐下降（P〈0．05—0．0001）;慢性亚急性肝衰竭（SACLF）患者早中期TGF—β1水平低于晚期（P〈0．05）;死亡患者血清TGF-β1水平明显高于病情好转者（P〈0．005）。结论：LF患者血清TGF-β1的水平与疾病的严重程度及预后密切相关,它们在LF发生发展过程中可能起重要作用。     

自微乳制剂的研究进展

目的通过查阅文献,对自微乳的制法、处方的优化及代表性研究实例三个方面,作一客观的综述。     

hSav1蛋白高表达对Mst1介导的细胞凋亡的影响

目的 观察hSav1蛋白高表达对Mst1介导的细胞凋亡的影响,探讨hSav1在Mst1介导的细胞凋亡中的作用.方法 构建蛋白hSav1的载体pCMV-HA-hSav1与蛋白Mst1的载体pcDNA/4TO-Flag-Mst1,共转染HeLa细胞.采用相应抗体做二者的免疫共沉淀,以证实蛋白hSav1和Mst1之间的相互作用;应用细胞免疫荧光共定位,进一步证实二者之间的相互作用.在HeLa细胞中,分别单独或同时转染质粒pcDNA/4TO-Flag-Mstl和pCMV-HA-hSav1,转染36 h后,加入凋亡诱导剂顺铂(50 μmol/L)作用14 h.应用磷脂结合蛋白碘化丙啶(Annexin V/PI)法检测细胞凋亡,观察蛋白hSav1高表达对Mst1介导的细胞凋亡的影响.结果 载体pCMV-HA-hSav1与pcDNA/4TO-Flag-Mst1构建成功,测序结果表明无突变或缺失.免疫共沉淀结果显示,蛋白hSav1可以在Mst1抗体的免疫沉淀物中检测出来,蛋白Mst1可以在hSav1抗体的免疫沉淀物中检测出来.细胞免疫荧光的结果显示,二者的荧光在细胞内存在共定位,并可充分融合.HeLa细胞中,单独Mst1蛋白高表达组的细胞凋亡率为24.5%±2.4%,单独hSav1蛋白高表达组与对照组比较,无明显细胞凋亡.蛋白Mst1和hSav1高表达组的细胞凋亡率为39.3%±4.0%,差异有统计学意义(P<0.05).结论 蛋白hSav1与Mst1在HeLa细胞内存在相互作用,蛋白hSav1高表达可明显促进由蛋白Mst1介导的细胞凋亡.     

滤泡性淋巴瘤的侵袭性转化及预后分析

目的:探讨滤泡性淋巴瘤(FL)侵袭性转化和预后的相关因素。方法:收集2015年8月至2021年1月本院收治的168例FL患者的临床资料,分析各指标在组织学转化(HT)及预后中的意义。结果:病理3级、Ki-67≥40%、β₂-MG>3 mg/L、LDH>245 U/L、POD24、骨髓未受侵犯与HT相关。单因素分析结果显示,FLIPI-2高危、病理3级、Ki-67指数≥40%、贫血、β₂-MG>3 mg/L、LDH>245 U/L、HT是影响PFS的不良因素;β₂-MG>3 mg/L、LDH>245 U/L、 POD24、HT是影响OS的不良因素。Cox多因素分析结果显示,β₂-MG>3 mg/L、HT是PFS的独立危险因素;HT是OS的独立危险因素。结论:FL中病理分级、Ki-67指数、β₂-MG、LDH、POD24及骨髓累及与否可预测HT风险,同时HT、β₂-MG>3 mg/L与FL预后不良有关。     

高表达蛋白hSav1对人胚肾HEK293增殖能力的影响

Objective To elucidate the effects of human Salvador 1 (hSav1 ) on cell proliferation of human embryonic kidney cell line HEK293. Methods The plasmid CFP-N1-hSav1 was constructed and transfected into HEK293 cells with lipofectamine 2000. The transfection efficiency was detected by fluorescent microscopy. The effects of hSav1 on cell proliferation were measured by MTT and BrdU incorporation. Results The transfection efficiency was about 70% -80%. The MTT results showed that the inhibition rate of cell proliferation in transfected group at the 12th, 24th, 36th, and 48th h was 2% , 5% , 15% , and 23% , respectively;while that in the control group was 2% , 3% , 2% , and 2% respectively. The BrdU incorporation revealed that the BrdU incorporation rate in transfected group (12. 9 ±5. 3)% was significantly lower than in control group (27.3±3.8)% (P<0.05). Conclusion hSav1 is a newly identified protein that can cause cell proliferation inhibition in HEK293 cells.