Box-Behnken设计优选藏药坐珠达西供试品溶液制备条件

目的采用Box-Behnken设计响应面法优化藏药坐珠达西的供试品溶液的制备条件,确定最佳的提取制备方法。方法选取加甲醇量、超声时间、超声温度为考察因素,以没食子酸、苯甲酸、山柰酚、木香烃内酯、去氢木香烃内酯、绿原酸、西红花苷Ⅰ和西红花苷Ⅱ8种成分量的总评归一值(OD)为指标,采用Box-Behnken设计优化坐珠达西样品的提取制备条件。结果最佳提取制备工艺为加甲醇15 m L,超声30 min,超声温度为80℃,重复3次试验,各成分量的RSD在1.1%~2.7%范围内。结论优选的提取制备条件稳定可行,可为坐珠达西质量标准研究的样品提取制备提供参考。     

Erbin interacts with Sema4C and inhibits Sema4C-induced epithelial-mesenchymal transition in HK2 cells

Erbin, a member of Leucine-rich repeat and PDZ-containing protein family, was found to inhibit TGF-β-induced epithelial-mesenchymal transition （EMT） in our previous study. However, the mechanism of Erbin in regulating EMT is unclear. Semaphorin protein Sema4C, with PDZ binding site at C-terminal has been recognized as a positive regulator of EMT. Here, we aimed to examine the inter- action between Erbin and Sema4C. HK2 cells were treated with TGF-β1, or transfected with Erbin and （or） Sema4C. Interaction of Erbin and Sema4C was identified by immunoprecipitation. RT-PCR was used to detect the expression of Erbin and Sema4C at mRNA level after transfection. The expression levels of Erbin, Sema4C, and markers of EMT were measured by using Western blotting or ELISA. Af- ter HK2 cells were stimulated with 10 ng/mL TGF-β1 for 72 h, the protein expression levels of Erbin and Sema4C were both up-regulated, and immunoprecipitation results showed Erbin interacted with Sema4C in HK2 cells both at endogenous and exogenous levels. Furthermore, overexpression of Sema4C suppressed E-cadherin, induced vimentin and promoted fibronectin secretion, indicating Sema4C promotes the process of EMT. However, HK2 cells overexpressing Erbin were resistant to Sema4C-induced EMT. In contrast, Erbin specific siRNA promoted EMT induced by Sema4C. Taken together, these results suggest that Erbin can interact with Sema4C, and co-expression of Erbin blocks the process of Sema4C-induced EMT.     

Box-Behnken设计及正交设计优选复方龙砂颗粒提取工艺

目的优选复方龙砂颗粒的提取工艺。方法采用响应面法,以挥发油体积为响应值,选取加水量、浸泡时间和蒸馏时间三个因素进行中心组合试验优化挥发油提取工艺;采用正交试验设计,以黄芩苷量和干膏率二者的综合评分值为指标,对挥发油提取残渣与地龙等其余6味药材合并后所进行的提取工艺中的加水量、提取时间和提取次数进行优选。结果挥发油提取最佳工艺为加10倍量水,浸泡36 min,蒸馏提取5.5 h;水提取最佳工艺为提取3次,每次加水10倍,提取1 h。结论实验合理的利用了两个设计方案的特点,所优选的提取工艺稳定可行。     

高血压与心律失常相关性研究的进展

高血压是心血管疾病最常见的危险因素,研究表明高血压和心律失常之间存在复杂的相互作用,与多种房性和室性心律失常的发生、发展有关。本文总结了有关高血压与心律失常相关性的现有文献,对高血压与心房颤动、室性心律失常、心源性猝死的流行病学、发病机制和临床意义进行综述。     

混合痔外剥内扎术中痔核根部两种结扎方法的临床效果比较

目的 为了减少术后的并发症,观察混合痔外剥内扎术中痔核根部两种结扎方法的临床治疗效果,对1048例混合痔患者采用0号丝线单纯痔核根部(治疗1组),1127例采用0号丝线缝扎和加固结扎痔核根部(治疗2组).对两组伤口愈合时间、结扎线脱落时间、伤口感染率进行比较.结果显示:治疗1组结扎线脱落时间3～7天,伤口愈合时间13～37天,平均17.4天;伤口感染37例,占3.53%;原发性大出血2例,占0.19%;继发性大出血1例,占0.10%.治疗2组结扎线脱落时间3～22天,伤口愈合16～53天,平均27.6天,伤口感染57例,占5.06%;原发性大出血3例,占0.26%;继发性大出血11例,占0.98%.结果表明:在混合痔外剥内扎术中以0号丝线单纯结扎痔核根部是安全的、可靠的.可以减少伤口感染的机会,结扎线脱落快而安全,可减少痔术后伤口感染和痔后继发性出血,缩短伤口的愈合时间.     

冠心病临床诊断价值分析

[目的]比较冠心病的3种常规临床诊断方法:典型的胸痛症状、静息心电图(electrocardiogram,ECG)、心肌肌钙蛋白Tn对冠心病的诊断价值. [方法]选择70例可疑冠心病患者,在就诊后1周内先后接受ECG、心肌肌钙蛋白Tn和冠状动脉造影(coronary arteriongraphy,CAG)检查,以冠状动脉造影为标准,70例患者被分为冠心病组(n=51)和非冠心病组(n=19),分别分析ECG、心肌肌钙蛋白Tn和发作性胸痛对冠心痛的检出率及其诊断的特异性、准确性等指标. [结果]胸痛、ECG和心肌肌钙蛋白Tn诊断冠心病的敏感性分别为56.86%、21.57%、88.24%;特异性分别为42.11%、63.16%、89.47%;准确性分别为52.86%、32.86%、88.57%. [结论]ECG、心肌肌钙蛋白Tn是诊断冠心病的安全、无创伤、重复性强且较准确的检查方法,如果将以上3项指标联合运用,将会在很大的程度上提高冠心病的诊断正确率.     

幽门螺杆菌感染与脑卒中的相关性研究

目的：探讨幽门螺杆菌（Hp)感染与脑卒中的关系。方法：应用酶联免疫法检测135例脑卒中患者和60例对照组血清Hp细胞毒素相关蛋白A IgG抗体（CagA-Hp-IgG)及血清总胆固醇（TC）、甘油三酯（TG）、载脂蛋白A（Apo-A)、载脂蛋白B（Arpo-B)、高密度脂蛋白胆固醇（HDL－C），低密度脂蛋白胆固醇（LDL－C）浓度。结果：观察组血清CagA-Hp-IgG阳性率（57．8％）显著高于对照组（35.0％）。CagA-Hp-IgG阳性与阴性者间血脂水平比较差异无显著性（P＞0．05）。结论：Hp感染与脑卒中可能有明显差异；其可能的发病机制不明。     

复方红景参丸治疗慢性阻塞性肺疾病临床研究

目的 探讨复方红景参丸治疗慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)的临床效果。方法 选取2019年7月—2020年6月本院收治的COPD患者60例,按照随机数字表法随机分为2组,每组30例。对照组患者规律吸入布地奈德福莫特罗粉吸入剂,观察组患者在对照组治疗基础上予以复方红景参丸口服。比较2组患者治疗前后慢性阻塞性肺疾病评估测试评分(COPD assessment test,CAT)、中医症状积分、肺部啰音评分、肺功能水平、血气分析及炎症指标水平,比较2组患者28d内COPD急性加重次数。结果 治疗后,2组患者CAT积分、中医症状积分、肺部啰音积分、PaCO2、WBC、NEU%及CRP水平均明显降低,FEV1%预计值、FEV1/FVC及PaO2水平均明显升高(P<0.05);且观察组患者CAT积分、中医症状积分、PaCO2、WBC及CRP水平显著低于对照组,FEV1%预计值、FEV1/FVC、PaO2水平显著高于对照组(P<0.05)。结论 复方红景参丸辅助治疗COPD患者可显著控制临床症状,有效改善患者呼吸功能,降低机体炎症水平和COPD急性加重次数,具有良好的临床推广价值。     

GC-MS法分析藏药坐珠达西中的化学成分

目的 GC-MS法分析藏药坐珠达西(肉豆蔻、木香、藏红花等)中的化学成分。方法甲醇超声提取该药物后,GC-MS法分析其中成分,面积归一化法计算其相对含有量,对结果进行直观分析、主成分分析、聚类分析。结果9批坐珠达西样品可分为4类,有19种成分,含有量差异明显。其中,油酸、肉豆蔻酸、榄香素、大匙叶桉油烯醇、巴西菊内酯、桉叶油二烯-5,11(13)-8,12-内酯、去氢风毛菊内酯为特征性成分。结论 4个厂家坐珠达西样品间差异较大,故应进一步完善该药物及其原药材的质量标准。     

Erbin在肾间质纤维化中的表达和作用

Objective To investigate the expression of Erbin in renal interstitial fibrosis (RIF) and the effect of over-expression of Erbin on transforming growth factor β1 (TGF-(β1)-induced epithelial-mesenchymal transition (EMT) in NRK52E cells. Methods In vivo, the model of renal fibrosis was induced by 5/6 subtotal nephrectomy in rat. Scr and BUN was detected and Masson staining was used to evaluate the level of renal tissue fibrosis. The location and expression of Erbin in renal tissue were detected by immunohistochemistry and Western blotting. In vitro, after NRK52E cells were treated by TGF-β1 (10 μg/L) for 72 h, immunofluorescence and Western blotting were used to obverse the expression and distribution of E-cadherin and α-SMA. The expression of Erbin mRNA and protein were detected by RT-PCR and Western blotting respectively. NRK52E cells were transiently transfected with Prk5-myc-Erbin plasmid via lipofectamine 2000, then the expressions of Erbin, E-cadherin and α-SMA were detected by Western blotting. Results (l)Compared to sham group with Scr (33.96±7.28) μmol/L and BUN (8.11±2.55) mmol/L, rats in 5/6 nephrectomy model with Scr (140.52±61.11) μmol/L and BUN (34.23±7.66) mmol/L revealed renal dysfunction. Masson staining indicated kidney interstitial fibrosis, and the expression of Erbin was significantly increased in renal tissue(2.9 folds), especially in tubular epithelia. (2)In vitro, the expressions of Erbin and α-SMA were markedly increased (2.3 folds and 2.1 folds, P<0.05, respectively) and the expression of E-cadherin was dramatically decreased in NRK52E cells stimulated by TGF-β1, which were consistent with immunofluorescence results. TGF-β1-induced E-cadherin suppression and a-SMA induction could be efficiently blocked by over-expression of Erbin (all P <0.05). Conclusions Erbin is up-regulated in renal interstitial fibrosis, and over-expression of Erbin can partly inhibit renal EMT induced by TGF-β1, which indicates Erbin playing an protective role in renal fibrosis.