药铜宫内节育器的恒河猴实验研究

实验选用恒河猴放置药铜宫内节育器（ＬＮＧ－Ｃｕ－ＩＵＤ：ＬＮＧ６μｇ／ｄ，Ｃｕ２００ｍｍ２）６个月，以观察其节育效果及月经情况。结果：节育效果好，无妊娠、无脱落；月经改变表现为出血不规则、经量减少及闭经，但取器后即恢复正常；宫腔冲洗液ＰＧｓ放免测定，置器前后ＰＧＥ２、ＰＧＦ２α、６－ｋｅｔｏ－ＰＧＦ１α浓度无显著差异，ＴＸＢ２明显上升，６－ｋｅｔｏ－ＰＧＦ１α／ＴＸＢ２比值下降，提示ＬＮＧ－Ｃｕ－ＩＵＤ导致周期出血减少可能与此有关；置器后子宫内膜上皮细胞和若干酶活性不同程度受抑。结果提示：兼释ＬＮＧ和Ｃｕ２＋的ＩＵＤ具有节育效果可靠、经血量少等优点，但对月经的影响尚需深入研究。     

Expression of Nuclear Factor-κB in Mouse Uterus during Peri-implantation

To investigate the expression of the subunit p65 of NF-κB and inhibitor kappa B alpha (IκBα) in mouse uterus during peri-implantation, thereby investigating whether transient activation of nuclear factor-κB (NF-κB) takes place during embryo implantation in mice. Immunohistochemical technique was used to examine the expression and localization of p65 in endometrium or deciduas, and Western blot analysis was employed to detect the levels of IκBα protein in mouse endometrium or deciduas. P65 protein was detected in stromal cells, epithelial cells of endometrium as well as in myometrium. Staining was predominately seen in the cytoplasm of the cells. Staining intensity for p65 was stronger in the epithelial compartment than the stromal compartment and myometrium. Staining intensity increased slightly during pregnancy, and it reached a high level on pregnancy day 5 and day 8. In contrast to p65, the level of IκBα protein was lowest on pregnancy day 5 in all groups. Our results suggested that NF-κB may regulate embryo implantation by its transient activation in mice.     

类固醇激素与年龄有关的变化

类固醇激素的产生是以多变性为其特征。如皮质类固醇的产生在一天24小时内有变化,随月经周期而每月变化,以及在一生中各个时期如青春期、更年期、男性更年期、肾上腺激素初现期、肾上腺激素停止期亦均有变化等。类固醇激素全部以类固醇核为基础,主要从胆固醇衍生而出。不同的类固醇分子——皮质类固醇、雄激素、雌激素和孕酮,其区别在于不同侧链的数、性质和位置,而有关类固醇环结构中双键位置的变化则相对较     

表面修饰对镁合金WE42生物相容性的影响

目的:评价微弧氧化(MAO)及聚左旋乳酸(PLLA)封孔表面修饰对生物可降解WE42镁合金材料的生物相容性的影响.方法:以微弧氧化法制备陶瓷膜表面的WE42镁合金材料(MAO/WE42),再在陶瓷膜膜层表面进行PLLA封孔处理,制成MAO+PLLA修饰WE42镁合金材料(MAO+PLLA/WE42);扫描电镜观测材料在体外生理环境中的表面微观形貌;用材料浸提液处理人脐静脉内皮细胞,MTT比色法检测细胞相对增殖率(RGR),判断细胞毒性的级别;同时测定与材料表面接触2 min后血浆的凝血时间(PT、RT)及经材料浸提液处理的2%人血悬液的溶血率(hemolytic ratio).结果:扫描电镜观察显示WE42浸泡后出现严重腐蚀,MAO/WE42和MAO+PLLA/WE42材料浸泡前后变化较轻,未出现明显的腐蚀孔洞;WE42细胞毒性较强,而MAO/WE42、MAO+PLLA/WE42无明显细胞毒性作用;MAO/WE42、MAO+PLLA/WE42具有一定的抗凝血性能;溶血试验结果显示,MAO/WE42和MAO+PLLA/WE42的溶血率均低于WE42,且MAO+PLLA的抗溶血性能优于MAO(P < 0.01).结论:微弧氧化表面修饰可改善WE42的生物相容性,PLLA封孔处理可提高WE42的抗溶血性能,MAO+PLLA作为可控吸收镁合金药物洗脱支架的载药层是安全的.     

直接PCI术中严重无复流1例报告

患者男,63岁.因间断胸痛3d、持续加重3h于2012年8月24日入院.入院前2d间断出现心前区疼痛,伴胸闷、气促、大汗,症状持续数分钟自行缓解,每天发作1～3次.本次入院前3h症状持续加重,遂来我院就诊.患者否认有高血压、糖尿病史;吸烟30年,10支/d.查体：体温36.5℃,脉搏79次/min,呼吸16次/min,血压150/90 mmHg.双肺呼吸音清,未闻及干、湿性罗音.心界不大,心率70次/min,未闻及杂音.实验室检查：WBC 12.99×109/L,肝肾功能和电解质基本正常,B型脑利钠肽（BNP）65.1 pg/mL,肌酸激酶（CK）1 389.0 U/L,肌酸激酶同工酶（CK-MB） 110.0 U/L,超敏肌钙蛋白Ⅰ 23.271 ng/mL.心电图：窦性心律,W1～4导联ST段弓背上抬0.05 ～0.1 mV.入院诊断：冠心病,急性前壁心肌梗死,心功能Ⅰ级（Killip分级）.立即给予阿司匹林300 mg、氯吡格雷600 mg口服.急诊冠状动脉造影显示左主干无狭窄,前降支近中段狭窄99％,TIMI血流2级;第2对角支开口80％狭窄,分叉病变;右冠近段局限80％狭窄,回旋支无严重狭窄.PCI术中行梗死相关动脉血运重建,使用Crossover单支架术式,对角支以导丝保护,前降支近中段以9个大气压释放3.5 ×33 mm支架,造影显示明显支架膨胀不良;使用3.5 ×15 mm后扩球囊,给予12个大气压扩张,显示支架膨胀良好,但前降支远端TIMI 1级血流.     

阿司匹林通过转化生长因子-β1途径抑制人脐动脉平滑肌增殖的实验研究

目的:探讨阿司匹林对人脐动脉平滑肌细胞(HUASMCs)增殖的影响及转化生长因子(TGF)-β1信号途径在其中可能发挥的作用。方法:利用HUASMCs进行体外培养;通过MTT法观察不同浓度阿司匹林(0.5、1.0、2.0及5.0mmol/L)以及加入TGF-β1中和抗体后对细胞增殖(OD值)的影响;采用流式细胞技术检测阿司匹林(2.0mmol/L)以及加入TGF-β1中和抗体后对细胞周期的影响。结果:不同浓度阿司匹林(0.5、1.0、2.0及5.0mmol/L)及加入TGF-β1中和抗体培养HUASMC细胞后,其数量(OD值)组间、时间效应差异均有统计学意义(P<0.05),且组间与时间效应间存在交互作用(P<0.05)。阿司匹林(2.0mmol/L)可使HUASMCs的增殖阻滞在G0/G1期(P<0.05),TGF-β1中和抗体可使A2.0组的细胞G0/G1期减少,S期增多(P<0.05)。结论:阿司匹林可使HUASMCs的增殖阻滞于G0期从而抑制HUASMCs增殖,这种抑制作用可能在一定程度上是通过TGF-β1信号途径所介导的。     

正常范围甲状腺激素水平与冠状动脉Gensini评分的相关性分析

Abstrat：Objective To study the correlation between normal level of thyroid hormone and gensini coronary scoring system．Methods 233 patients who were treated with CAG and diagnosed of patients with coronary heart disease were divided into 4 groups based on gensini coronary scoring system ,all the patients were determined kinds of index which include triiodothyronine(T3),thyronine(T4),free thyronine(FT4), free triiodothyronine (FT3), thyroid stimulating hormone (TSH) and correlation between thyroid hormone level and gensini coronary scoring system was studied by Spearman correlation analysis and Multiple regression analysis.Results No significant difference was found in the levels of triiodothyronine(T3),thyronine(T4),freethyronine(FT4),Cholesterol(CHO)andTriglyceride(TG) between the 4 groups(P>0．05).FT3 and HDL-C decreased with the increase of the degree of coronary artery lesions（P<0.01）, AGE and LDL-C increased with the increase of the degreeof coronary artery lesions(P<0.05),there was statistical significance.Negative linear correlation was found between the gensini coronary scoring system and FT3、HDL-c,was positively correlated with age and LDL-C .both the index Was independently correlated to Gensini integral.Conclusion the lower level of FT3 is an indepent risk factor for lesion severity of CHD．