In vitro differentiation of human adipose-derived adult stromal cells into neuron-like cells in hippocampal astrocyte conditioned medium

BACKGROUND: At present, researches on differentiating from human adipose-derived adult stromal cells (hADASC) to neuron-like cells are focus on inducing by artificial-synthetic compound solution; however, hippocampal astrocyte conditioned medium (HCAM) can induce in vitro differentiation from hADASC to neuron-like cells is still unclear. OBJECTIVE: To observe whether HCAM can induce in vitro differentiation from hADASC to neuron-like cells. DESIGN: Randomized control study. SETTING: Department of Neurology, Taixing People's Hospital; Central Laboratory, North China Coal Medical College. MATERIALS: Donor of adipose tissue was donated by female volunteers suffering from caesarean section in the department of obstetrics & gynecology in our hospital and aged 20－35 years. Adipose tissue was collected from subcutaneous tissue of abdomen during the operation. In addition, 8 male newborn Wistar rats within 24 hours with average body mass of 20 g were provided by Animal Institute of Chinese Academy of Medical Sciences. Rabbit-anti-human Nestin polyclonal antibody, rabbit-anti-human glial fibriliary acidic protein (GFAP) polyclonal antibody, rabbit-anti-human neuro-specific enolase polyclonal antibody and mouse-anti-human microtubal associated protein 2 (MAP-2) polyclonal antibody were provided by Wuhan Boster Company. METHODS: The experiment was carried out in the Central Laboratory of North China Coal Medical College from October 2004 to June 2005. hADASC was cultured with HCAM and its growth and morphological changes were observed under inverted phase contrast microscope. Immunocytochemistry, immunofluorescence and Western blotting were used to evaluate the expressions of Nestin, which was a specific sign of nerve precursor, neuro-specific enolase and MAP-2, which was a specific sign of nerve cell, and GFAP, which was a specific sign of neuroglial cells. MAIN OUTCOME MEASURES: Nestin, which was a specific sign of nerve precursor, neuro-specific enolase and MAP-2, which was a specific sign of nerve cell, and GFAP, which was a specific sign of neuroglial cells. RESULTS: On the 3rd day of culture, partial hADASC started deformation from slender shuttle-shape cells to neuron-like cells. It suggested that cells stretched out apophysis, which were mainly double-pole or multiple-pole cells. Five days later, immunohistochemical detection suggested that expression of Nestin (10.5±0.037) was found out in cells; meanwhile, expressions of GFAP (38.4±0.052) and neuro-specific enolase (NSE) (15.7±0.023) were also found out in cells; however, expression of MAP-2 was not observed. Western blot indicated that, 5 days after effect of HCAM, Nestin was found out in hADASC; meanwhile, expressions of GFAP and neuro-specific enolase were also found out; however, expression of MAP-2 was not observed. CONCLUSION: HCAM can induce the differentiation from hADASC to neuron-like cells in vitro.     

聚丙烯酰胺水凝胶注射隆乳应用体会

目的 探索准确的乳腺后间隙注射聚丙烯酰胺水凝胶隆乳的方法，减少并发症的发生。方法 采用长钝针穿刺，进入乳腺后间隙，一点注射麻醉药及水凝胶。结果 为869例患者行聚丙烯酰胺水凝胶注射隆乳，渴意率达98．3％。结论 准确的注射层次和及时正确的处理并发症，是聚丙烯酰胺水凝胶注射隆乳术的成功保证。     

子宫黏膜下肌瘤行膨宫泵等离子电切术的手术配合

目的 探讨子宫黏膜下肌瘤行膨宫泵和等离子电切术的配合要点。方法 对30例子宫黏膜下肌摘患者使用膨宫泵和等离子电切手术。结果 30例手术顺利．均1次完成切除，平均手术时间40min，平均住院5d出院,无1例发生并发症。结论 术前心理护理、设备准备，术中正确摆放体位、熟练应用专科器械是护理配合的要点。     

老年病人中α1—肾上腺素能阻滞剂的合理应用

本文介绍了老年人高血压伴充血性心衰、左室肥厚、糖尿病、高脂血症、呼吸道疾病和良性前列腺增生时α_1-肾上腺素能阻滞剂的合理应用。     

世界健康教育发展的新趋势

世界健康教育发展的新趋势毕连华，戴梅竞，黄治平健康教育是由现代医学、心理学、卫生学、社会医学、儿童少年卫生字、教育学、行为科学、艺术学、社会学等多种学科组成的综合性应用学科，健康教育作为现代医学的一个新型交叉学科，从其诞生之日起，就给医学带来了巨大的...