磁共振扩散加权成像与常规序列在早期股骨头缺血坏死诊断中的应用

目的：比较磁共振扩散加权成像（DWI）与常规序列对早期股骨头缺血坏死的检测差异,并探讨DWI的参数设置及病理基础。方法使用GE Signa HDx 1．5T磁共振对61例可疑早期股骨头缺血坏死患者行自旋回波T2加权像（SE T2WI）、自旋回波T1加权像（SE T1WI）及DWI,其中26例（36髋）证实为坏死,35例（70髋）为正常,然后通过工作站对数据进行处理,分析扩散敏感度b值分别为200、400、600、800 s/mm2时DWI信号强度、表观弥散系数（ADC）,对比DWI、SE T1WI及SE T2WI检测结果。从而显示早期股骨头缺血坏死的敏感性。结果当b值取400 s/mm2时,最有利于图像分析及诊断。缺血坏死组平均ADC值为（12．5±1．4）×10-4 mm2/s,健康组为（2．7±1．2）×10-4 mm2/s,健康组的ADC值明显小于坏死组（P＜0．05）。 DWI、SE T1WI与SE T2WI分别检出早期股骨头缺血坏死36髋（100％）、32髋（89％）和30髋（83％）。3种检查差异有统计学意义（P＜0．05）。结论应用DWI有利于早期股骨头缺血坏死的鉴别,与常规序列对比,可提高早期股骨头缺血坏死诊断的准确性。     

自噬在胶质母细胞瘤发生发展中的作用

自噬是细胞内维持稳态和保护细胞在应激条件下免于死亡的过程,通过溶酶体途径降解受损细胞器及错误折叠的蛋白质发挥作用.自噬在胶质母细胞瘤(GBM)中发生频繁,可导致长期化疗后多药耐药(mul-tidrug resistance,MDR).自噬在胶质瘤及MDR细胞中发挥双重作用,既参与其发生发展,同时也能清除凋亡抑制的肿瘤细...     

幼年和成年大鼠外周血T细胞亚群变化

本实验应用了MAbs和流式细胞仪做为检测方法,比较观察了不同鼠龄的大鼠外周血T细胞亚群分布变化。结果显示,幼鼠(40d)和成鼠(5～6个月)的外周血T细胞亚群变化有明显差异:成鼠的OX-19~+(全T细胞)和W3/25~+(Th/i)细胞细胞较幼鼠有明显减少(P<0.001),而OX-8~+(Ts/C细胞)亚群却较幼鼠明显升高。但W3/25~+和OX- 8+亚群细胞表面的荧光密度变化却与阳性细胞百分率相反。推测造成这种T细胞亚群变化的原因可能与T细胞的发育成熟及T细胞本身的调节有关。实验资料为研究大鼠T细胞亚群的发育成熟过程提供了重要数据。     

新型全固态双膜钙离子选择电极的研制

将电化学可逆性好、化学稳定性高的聚苯胺通过电化学方法修饰于铂丝表面，进一步以土霉素合钙（Ⅱ）为电活性物质制作成全固态钙离子选择电极，用于生物试样的测定，与原子吸收光度法对照，结果令人满意。材料和方法仪器和试剂ＰＨＳ－２型酸度计，ＺＦ－３恒电位仪，ＶＩ...     

利福平减低心痛定降压效果1例

1 病例报告患者女72岁,因腹部饱满,食欲不佳,于一九九○年十二月入院.病人有五年原发性高血压史,长期以来服用心痛定40毫克,每天2次病情控制良好.查体:体温36.2℃,脉搏84次/分,呼吸22次/分,血压156/86毫升汞柱.实验室:红细胞沉降率92毫米/小时.C-反应2.1毫克/升,免疫球旦白G2890毫克/分升.血清腺苷酶26.6单位/毫升.结核菌素试验( )(14mm).胸     

1-(5''''-溴阿魏酰基)-4-苄基哌嗪盐酸盐类似物体外清除羟自由基活性研究

目的:研究1-(5'-溴阿魏酰基)-4-苄基哌嗪盐酸盐类似物(BFP,L1～9)在体外清除羟自由基(·OH)的活性.方法:采用邻二氮菲-Fe2 氧化法测定BFP、阿魏酸(FA)、抗坏血酸(Vc)在体外对Fenton反应产生·OH的清除能力,计算表观清除率(CL).结果:L5的最大·OH表观清除率(CLmax)最大,L8最小,L1、L3、L5、L7的CLmax都比FA、Vc大.结论:BFP苄基苯环上2-Cl、3-Cl、2-CH3、4-CH3、3-OCH3取代,可增强体外清除·OH活性;3-CH3取代,体外清除·OH活性基本不变;2-OCH3、4-OCH3取代,体外清除·OH活性降低.     

性腺疾病应早治

睾丸是男性的重要生殖器官，也就是性腺，主要功能是产生精子和雄性激素，它决定着男孩长大成人后有无正常的性功能和生育能力。作为家长，从男孩降生后的第一声啼哭起，就要小心抚摸他的阴囊．查看一下阴囊内是否有两个如小花生大小的睾丸。     

4-羟基肉桂酸衍生物清除羟自由基活性的构效关系

目的:研究4-羟基肉桂酸(4-HCA)衍生物清除羟自由基(.OH)活性并初步分析其构效关系。方法:用邻菲罗啉化学发光法测定4-HCA衍生物清除.OH活性。结果:化学结构不同的4-HCA衍生物体外清除.OH的能力有较大差异。结论:4-HCA苯环上3、5位取代基的引入可以明显地影响此类化合物抗.OH活性,甲氧基和乙氧基可明显提高活性,溴次之,硝基则降低活性。     

单倍型供者淋巴细胞输注治疗复发性急性白血病

Objective To evaluate the effect of haploidentical lymphocyte infusion on refractory and relapse acute leukemia. Methods The haploidentical donor lymphocyte infusion was used to treat for relapse acute myeloid leukemia 3 patients (M2 2 eases, M4 lcase), one relapse acute lymphocyte leukemia from April 2006 to October 2007. Four cases who had accepted secondly regimens were ineffective,after relapse. Collecting donor lymphocytes, parents children as donor supply in 3 cases, mother as donor supply one case. Before donor lymphocyte infusion patients received chemotherapy of different regimens. Donor haploidentical iymphocytes irradiated by 6-8 Gy radial were infused when patients white cell count was at the lowest after the chemotherapy. The average of infusion cells was 2.3 (1.4-3.1)×108/kg. Results One patient acquired complete remission and two patients were effective in three relapse acute myeloid leukemia. It was ineffective in relapse acute lymphocyte leukemia. No transfusion related graft versus host disease was observed. One patient has had herpes zoster virus infection. Conclusion Haploidentical donor lymphocyte infusion with chemotherapy are effective for refractory and relapse in acute myeloid leukemia, but the infused cell quantity and irradiated dosage must be further discussed.     

单倍型供者淋巴细胞输注治疗复发性急性白血病

Objective To evaluate the effect of haploidentical lymphocyte infusion on refractory and relapse acute leukemia. Methods The haploidentical donor lymphocyte infusion was used to treat for relapse acute myeloid leukemia 3 patients (M2 2 eases, M4 lcase), one relapse acute lymphocyte leukemia from April 2006 to October 2007. Four cases who had accepted secondly regimens were ineffective,after relapse. Collecting donor lymphocytes, parents children as donor supply in 3 cases, mother as donor supply one case. Before donor lymphocyte infusion patients received chemotherapy of different regimens. Donor haploidentical iymphocytes irradiated by 6-8 Gy radial were infused when patients white cell count was at the lowest after the chemotherapy. The average of infusion cells was 2.3 (1.4-3.1)×108/kg. Results One patient acquired complete remission and two patients were effective in three relapse acute myeloid leukemia. It was ineffective in relapse acute lymphocyte leukemia. No transfusion related graft versus host disease was observed. One patient has had herpes zoster virus infection. Conclusion Haploidentical donor lymphocyte infusion with chemotherapy are effective for refractory and relapse in acute myeloid leukemia, but the infused cell quantity and irradiated dosage must be further discussed.