甘精胰岛素对游离脂肪酸诱导的RIN-m细胞凋亡的影响

[目的]研究甘精胰岛素(insulin glargine)对游离脂肪酸诱导的β细胞凋亡是否具有保护作用.[方法]不同浓度的甘精胰岛素、普通人胰岛素以及IGF-1与0.3 mmol/L棕榈酸分别加入RIN-m细胞培养液中,24h后,采用Hoechst33342染色、流式细胞仪以及ELISA方法检测RIN-m细胞的凋亡.[结果]0.3 mmol/L棕榈酸诱导RIN-m细胞凋亡率达42.10%±4.24%,同时加入甘精胰岛素或者普通胰岛素(均为100 nmol/L)的凋亡率分别为32.00%±3.08%和35.97%±3.14%,甘精胰岛素对凋亡的抑制作用明显强于普通胰岛素,并呈剂量依赖性.[结论]甘精胰岛素对游离脂肪酸诱导的β细胞凋亡具有呈剂量依赖性的保护作用.     

低氧与去铁敏对移植供肝作用的对比性研究

Objective To compare the protecting effect of hypoxia preconditioning(HP) and deferoxam-ine preconditioning (DP)on transplanted livers and to search a protecting method which is suitable for clini-cal practice.Methods SD rats are randomly divided into four groups:sham operation group (S),analogic transplantation group (AT),deferoxamine preconditioning group (DP) and hypoxia preconditioning group (HP).Twenty-four hours after operation,several factors of each group were measured respectively,inclu-ding deterioration of histomorphology,hepatic zymogram in the serum,hypoxia inducing factor-1 (HIF-1α)and malonaldehyde (MDA) in hepatic tissue.Results The levels of alanine aminotransferase (ALT),glu-tamic oxalacetic transaminase (AST) and alkaline phosphatase (ALP) in AT group,DP group and HP group were higher than S group's.Levels of ALT,AST,ALP in AT group were dramatically high,hepatic tissue suffers from edema,degeneration even necrosis.By contrast,the pathological change is milder in DP group and HP group.HIF-1α in hepatic tissue of AT group was lower than that in DP group and HP group,MDA was higher than two latters (P < 0.05).Conclusion DP can protect transplanted liver as same as HP,however,DP is more suitable for clinical practice.     

低氧与去铁敏对移植供肝作用的对比性研究

Objective To compare the protecting effect of hypoxia preconditioning(HP) and deferoxam-ine preconditioning (DP)on transplanted livers and to search a protecting method which is suitable for clini-cal practice.Methods SD rats are randomly divided into four groups:sham operation group (S),analogic transplantation group (AT),deferoxamine preconditioning group (DP) and hypoxia preconditioning group (HP).Twenty-four hours after operation,several factors of each group were measured respectively,inclu-ding deterioration of histomorphology,hepatic zymogram in the serum,hypoxia inducing factor-1 (HIF-1α)and malonaldehyde (MDA) in hepatic tissue.Results The levels of alanine aminotransferase (ALT),glu-tamic oxalacetic transaminase (AST) and alkaline phosphatase (ALP) in AT group,DP group and HP group were higher than S group's.Levels of ALT,AST,ALP in AT group were dramatically high,hepatic tissue suffers from edema,degeneration even necrosis.By contrast,the pathological change is milder in DP group and HP group.HIF-1α in hepatic tissue of AT group was lower than that in DP group and HP group,MDA was higher than two latters (P < 0.05).Conclusion DP can protect transplanted liver as same as HP,however,DP is more suitable for clinical practice.     

都叫肾炎，但“张冠”不能“李戴”

肾炎与通常所说的炎症不同，肾脏炎症尽管都叫肾炎，但其病因、病理、病程和预后等都不同，所以在预防、治疗、康复等方面也有很大的区别。     

结肠癌的多层螺旋CT检查

结肠癌的检查方法很多，过去主要依靠大便潜血试验、钡灌肠、结肠镜检查。近年来，随着多层螺旋CT的出现及CT仿真内窥镜等新技术的应用，使CT检查逐渐成为肠道肿瘤术前分期实用而可靠的方法。综述了结肠多层螺旋CT的检查方法、临床应用及其展望。     

肾炎因不同 治不同

与通常所说的发炎不同，肾脏发炎尽管都叫做肾炎，但是病因、病理、病程经过都有很大不同，所以在预防、治疗、康复、预后等环节，都有很大的区别。     

37例大面积烧伤的临床分析

我院烧伤科自1990年至2003年共收治烧伤面积达80％以上的大面积烧伤病人37例，我们对所有患者均采取及时复苏，快速大量补充液体，使患者平稳渡过休克期，对患者的预后起到了很好的作用。     

带蒂骨──骨膜肌瓣鼻腔粘骨膜下植入术治疗萎缩性鼻炎

上颌窦前壁骨──骨膜肌瓣植入鼻腔粘骨膜下治疗萎缩性鼻炎患者25例（38侧），通过术后6个月～3年观察，显效88％，总有效率100％。因移植自体带蒂骨──骨膜肌瓣有血液供应，骨质不会被吸收、坏死或脱落，故该疗法近期疗效显著，远期疗效巩固，无并发症。     

几种常见疾病高凝状态的探讨

高凝状态也称血栓前状态、血栓前综合征、易栓症等，主要指在有些疾病发展过程中，血液凝固性增高，有利于血栓形成，使原发病向恶化方面发展。高凝状态有原发性与继发性之分。原发性见于遗传性抗凝血酶Ⅲ（AT—Ⅲ）、蛋白C（PC）、蛋白S（PS）、肝素辅因子Ⅱ（HCⅡ）缺乏症，继发性者可见于多种血液及非血液疾病。高凝状态是当前国内外研究十分活跃和有争议的课题。我所自1989年以来先后通过6名硕士研究生对内科常见几种疾病进行了高凝状态的研究，综述如下。一、检测对象：共215例，其中急性白血病29例、慢性肺源性心脏病42例、冠心…     

牵拉对α1受体激动剂苯肾上腺素诱发兔离体血管收缩反应的影响

目的 研究在α₁受体激动剂苯肾上腺素(Phe)诱发血管收缩反应实验中，如何确定动脉标本的最适前负荷。方法 采用兔离体肾动脉、股动脉、隐动脉、肠系膜动脉、脾动脉和耳中央动脉环标本的等长张力纪录法。结果 在1.0～5.0 g前负荷条件下, 随前负荷增加, 各血管环静息张力呈线性增加。 KCl (120 mmol·L^-1)引起各动脉的收缩随前负荷的增加而增强，但除耳动脉外, 在其他动脉标本上未观察到KCl最大收缩反应的最终坪值点。以Phe（0.01～100 μmol·L^-1）为收缩剂时, 在肠系膜动脉, 脾动脉和隐动脉, Phe的EC₅₀(EC_50·Phe)值随前负荷增加(1.0～5.0 g)而明显改变; 但是, 各组标本在不同前负荷下的EC_50·Phe值中存在一个最小值，其标准差亦很小。结论 前负荷明显影响血管的EC_50·Phe值测定。确定离体血管标本最适前负荷, 特别是研究受体反应时, 应以受体激动剂的EC₅₀值及其变异程度最小作为关键指标。