Change of alveolar epithelial type Ⅱ cells and pulmonary surfactant protein A in young rats with acute lung injury

Objective To study the temporal changes of alveolar epithelial type Ⅱ cells and surfactant pro-tein A in young rats with acute lung injury induced by lipopolysaecharide. Method Totally 110 SD young rats (male:53, female : 57) were randomly divided into ALI and normal control groups (six subgroups in each group).LPS(4 mg/kg) was given intraperitoneally in ALI group. The same amount of normal saline was given in the con-trol groups. Eight rats in each subgroup were sacrificed at 6, 12, 24, 36, 48 and 72 hours after the injection.Lung samples were taken for transmission electron microscope examination. RT-PCR was epmloyed for the mea-surement of SP-A mRNA. Western blot was used for the detection of SP-A in the lung tissue. ANOVA and homo-geneity of variance test were performed by SPSS 12.0. Results The microvilli disappeared at 24 hours after the injection of LPS. The number of lamellar body (LBs) was provisionality increased at 24 hours and 48 hours. The ring-like an'angement of LBs around nucleus and the giant LB with vacuole-like deformity were found as the main characteristics of AEC- Ⅱ in ALI at 48 hours. The number of LBs reduced and broken and residual LB remained at 72 hours. SP-A elevated greatly from 24 to 48 hours (P < 0.01), reached peak at 36 hours (6.94 ± 0.80, P <0.01),reached the lowest level(3.87 ±0.50, P <0.01)at 72 hours. Conclusions The pathological changes of AEC-Ⅱ and SP-A in lung tissue wiht ALI are time-dependent. The typical alterations of AEC- Ⅱ occurs at 48 hours accompanied by the compensatory increase of SP-A. AEC- Ⅱ is seriously injuried with the typical changes of LBs and the diminishing of SP-A in lung tissue.     

归芪白术方联合奥沙利铂对胃癌荷瘤小鼠机体免疫炎症分子的影响及抑癌作用

目的:探讨归芪白术方联合奥沙利铂在IL-6/JAK2/STAT3通路中对MFC胃癌荷瘤小鼠的抑瘤作用及对炎症分子影响。方法:建立MFC胃癌荷瘤小鼠模型,随机分为模型组、奥沙利铂组、奥沙利铂加归芪白术方高、中、低剂量组,10只/组,另选10只健康小鼠作为空白组;各组小鼠经口灌胃给予相应药物,空白组、模型组给予生理盐水,连续治疗14 d。处死小鼠取脾脏、胸腺、肿瘤称重,计算脏器指数及抑瘤率;HE染色观察小鼠瘤体病理形态学变化;ELISA法检测小鼠血清中IL-6含量;RT-qPCR和Western blot分别检测瘤组织中IL-6、JAK2、STAT3 mRNA及IL-6、p-JAK2、p-STAT3蛋白含量;免疫组化法检测肿瘤组织中c-Myc、Cyclin D1蛋白。结果:各治疗组小鼠瘤体重量显著低于模型组(P<0.01),联合用药高、中剂量组瘤体重量明显低于奥沙利铂组(P<0.05,P<0.01);与空白组相比,模型组小鼠的脾指数、胸腺指数降低(P<0.01),与模型组相比,奥沙利铂组小鼠的脾指数、胸腺指数降低(P<0.01),与奥沙利铂组相比,联合用药高剂...     

Change of alveolar epithelial type Ⅱ cells and pulmonary surfactant protein A in young rats with acute lung injury

Objective To study the temporal changes of alveolar epithelial type Ⅱ cells and surfactant pro-tein A in young rats with acute lung injury induced by lipopolysaecharide. Method Totally 110 SD young rats (male:53, female : 57) were randomly divided into ALI and normal control groups (six subgroups in each group).LPS(4 mg/kg) was given intraperitoneally in ALI group. The same amount of normal saline was given in the con-trol groups. Eight rats in each subgroup were sacrificed at 6, 12, 24, 36, 48 and 72 hours after the injection.Lung samples were taken for transmission electron microscope examination. RT-PCR was epmloyed for the mea-surement of SP-A mRNA. Western blot was used for the detection of SP-A in the lung tissue. ANOVA and homo-geneity of variance test were performed by SPSS 12.0. Results The microvilli disappeared at 24 hours after the injection of LPS. The number of lamellar body (LBs) was provisionality increased at 24 hours and 48 hours. The ring-like an'angement of LBs around nucleus and the giant LB with vacuole-like deformity were found as the main characteristics of AEC- Ⅱ in ALI at 48 hours. The number of LBs reduced and broken and residual LB remained at 72 hours. SP-A elevated greatly from 24 to 48 hours (P < 0.01), reached peak at 36 hours (6.94 ± 0.80, P <0.01),reached the lowest level(3.87 ±0.50, P <0.01)at 72 hours. Conclusions The pathological changes of AEC-Ⅱ and SP-A in lung tissue wiht ALI are time-dependent. The typical alterations of AEC- Ⅱ occurs at 48 hours accompanied by the compensatory increase of SP-A. AEC- Ⅱ is seriously injuried with the typical changes of LBs and the diminishing of SP-A in lung tissue.     

扩大的血管周围间隙临床意义的研究进展

血管周围间隙（PVS）是中枢神经系统的正常结构,具有一定生理和免疫功能。扩大的PVS（EPVS）长期以来被视为良性改变,然而越来越多的证据显示EPVS与多种神经系统血管性疾病和退行性疾病相关,其在脑内的分布特点对疾病的性质有一定的提示意义。本文将就EPVS的发生机制、影像学评价及其与多种神经系统疾病的关系作以综述。     

两、三焦点牵张成骨在下颌骨缺损修复中的应用

目的：通过两焦点、三焦点对山羊下颌骨缺损进行牵张,比较两种牵张成骨方式的新骨成骨量。方法：将8只成年山羊随机分成两组,每组4只,分别采用两焦点和三焦点牵张成骨术进行骨缺损修复。牵张结束后固定8周,处死动物取牵张区新生骨组织标本进行X线检查、组织学检查、骨密度分析。结果：两实验组牵张区新骨生长的质无明显区别,在量的比较上,三焦点组X线检查、组织学检查、骨密度测定的结果均好于两焦点组,骨密度测定分析结果有统计学意义（P〈0.05）。结论：三焦点牵张成骨在大面积颌骨缺损的修复中新骨的成骨量好于两焦点牵张成骨组。     

心脑血管疾病痰瘀互结证述析

近年来,社会发展,科技进步,人们生活方式发生了巨大变化,疾病谱也发生了重大改变。引人关注的是中医病证也发生了变化。据张伯礼教授根据临床观察,痰瘀互结证显著增多,尤其在心脑血管疾病以及糖尿病、高脂血症等代谢性疾病中表现尤为突出。在治疗上,也易误治,而出现变证。因此,系统进行痰I互结证研究具有重要意义。笔者将就近十年心脑血管疾病的痰瘀互结证综述如下。     

突发公共卫生事件下医疗机构捐赠药品标准化管理探索与实践

目的 分析新冠肺炎疫情期间捐赠药品管理工作流程，为突发公共卫生事件发生时，医疗机构受赠药品管理工作提供应急管理模式。方法 结合我院对接受捐赠药品在选择、接收、验收、储存、出库、使用、监管等具体工作模式，制定切实可行的捐赠药品管理制度。分析捐赠药品使用及管理情况，优化捐赠药品管理工作流程。结果 捐赠药品管理制度具有合法、合理、实操性强的特点。受赠药品遴选均符合新冠肺炎疫情防疫所需；验收、储存做到质量严把关，建立专账，来源可追溯；规范受赠药品使用审批流程，去向可追踪。结论 制定了符合医疗机构实际工作需求的《捐赠药品管理制度》，提高了捐赠药品管理工作效率，保障药品的质量及合理使用。     

生脉注射液佐治有机磷中毒67例疗效观察

有机磷农药中毒是临床常见急症 ,至今仍有很高的死亡率。我科自 1999年 6月～ 2 0 0 1年 6月采用生脉注射液佐治有机体磷中毒患者 6 7例 ,取得了较好的疗效 ,现报告如下。1　临床资料1.1　一般资料　 12 7例围期入院病人 ,均按《现代内科治疗学》的诊断标准[1] 进行诊断和分型。随机分成治疗组和对照组。治疗组 6 5例 :男 2 8例 ,女37例 ,年龄 11～ 80岁 ,平均 37.1岁。重度 8例 ,中度 32例 ,轻度 2 5例。对照组 6 2例 :男 2 8例 ,女 34例 ,年龄 15～ 78岁 ,平均 36 .8岁。重度 5例 ,中度30例 ,轻度 2 7例。两组病人性别 ,年龄 ,分型等方面…     

呼出气一氧化氮预测慢性咳嗽患儿气道高反应性临床价值研究

目的分析慢性咳嗽患儿呼出气一氧化氮(Fe NO)水平与支气管激发试验结果的相关性,探讨能否应用Fe NO预测慢性咳嗽患儿气道高反应性。方法对于中国医科大学附属盛京医院小儿呼吸内科门诊就诊的276例慢性咳嗽患儿,进行Fe NO、肺功能和乙酰甲胆碱为激发剂的支气管激发试验检查。结果支气管激发试验阳性组患儿122例,阴性组154例,阳性组的Fe NO值为20.0×10-9(14.0×10-9~37.51×10-9),阴性组的Fe NO值为16.0×10-9(12.0×10-9~21.0×10-9),两者间差异存在统计学意义(P=0.000);为明确Fe NO对于支气管激发试验结果是否存在判别作用,绘制受试者工作特征曲线(ROC),Fe NO预测气道激发试验结果的最佳阈值为31.5×10-9,相对应的敏感性为28.7%,特异性为97.4%,阳性预测值89.5%,阴性预测值63.0%;Fe NO与FEV1%下降20%时吸入的乙酰甲胆碱累积剂量(PD20-FEV1)之间不存在剂量相关性。结论慢性咳嗽患儿的Fe NO值对于气道高反应性有一定的预测作用。当Fe NO31.5×10-9时预测支气管激发试验阳性的准确性较高,提示患儿存在气道高反应性;当Fe NO31.5×10-9时,不能对支气管激发试验阳性或阴性做出预测,须进一步行支气管激发试验以判断气道的反应性。