PEG—20000分离HDL亚组分胆固醇测定临床应用

<正> 高密度脂蛋白(HDL)主要有HDL_2和HDL_3两种亚组分。高甘油三酯、冠心病及高血压患者的低HDL—C血症主要为HDL_2—C降低,糖尿病患者的HDL—C降低和注射胰岛素后HDL—C增高也表现为HDL_2—C的改变。而患肝病时低HDL—C血症主要为HDL_3—C的降低。上述两种情况都有HDL—C降低,但亚组分的改变不同。本文在Kostner方法的基础上采用不同浓度的PEG—20000分离HDL及亚组分,经实验观察     

分光光度法测定红细胞嘌呤核苷磷酸化酶

应用Trinder反应建立终点分光光度法测定红细胞嘌呤核苷磷酸化酶(PNP)活性,操作简单、快速、灵敏、准确。对酶反应最佳条件进行了实验探讨,底物Km为64mm,最佳PH7.5,延滞期1分钟30秒,1.5～8分钟酶活性与反应时间成比例。用3.5mol/L硫酸铵溶液终止反应,效果好,显色稳定。批内CV2.51％～3.15％,批间CV2.69％～3.28％。35例正常人红细胞PNP活性为13.66±1.73u/ml红细胞。     

血清高密度脂蛋白—胆固醇测定法

<正> 临床实验室分离血清中高密度脂蛋白(HDL)多采用肝素—Mn~++、磷钨酸钠—Mg~++、聚乙二醇—6000(PEG—6000)等化学法。我们近年来,采用PEG—6000—磷硫铁法测定HDL—胆固醇,具有影响因素少、简便、微量、精确、价廉、不受高甘油三酯的影响等优点,并且与肝素—Mn~++法有较好的相关性(n=47、r=0.954)。现将方法报道如下。     

高密度脂蛋白—胆固醇在肝胆疾病中的临床意义

高密度脂蛋白-胆固醇(HDL-C)作为动脉粥样硬化的防御因子,其水平与动脉粥样硬化发病间呈明显负相关。测定 HDL-C 对预测冠心病发病危险性比测定其它血脂指标更可靠,现已普遍受到人     

大学生经验性回避、认知融合及正念与焦虑抑郁关系

目的了解大学生经验性回避、认知融合及正念与焦虑抑郁的关系以及正念在经验性回避与焦虑抑郁间的中介作用。方法于2014年12月采用整群抽样方法从北京和河南2所高校抽取大学新生366名,使用接纳与行动问卷第二版(AAQ-Ⅱ)、认知融合量表(CFQ)、正念注意觉知量表(MAAS)、焦虑自评量表(SAS)和抑郁自评量表(SDS)进行问卷调查。结果大学新生经验性回避为(19.48±7.62)分,认知融合为(28.97±10.84)分,正念为(66.32±10.98)分,焦虑为(31.27±6.95)分,抑郁为(34.82±7.70)分;经验性回避对焦虑、抑郁具有正向预测作用(β=0.429、0.459,均P0.001);正念在经验性回避与焦虑抑郁间具有部分中介作用(β=–0.198,–0.173;P0.01),中介效应分别占总效应的22.06%和18.80%。结论经验性回避对焦虑、抑郁具有正向预测作用,正念在经验性回避与焦虑抑郁间具有部分中介作用。     

精准肝切除在结直肠癌肝转移中的应用价值

目的 探讨精准肝切除在结直肠癌肝转移治疗中的应用价值.方法 回顾性分析2006年10月至2009年10月天津医科大学附属肿瘤医院收治的85例结直肠癌肝转移患者的临床资料.根据治疗方法分为常规组43例和精准组42例.常规组:术前常规检测评估肝肾功能,增强CT和B超检查评估肿瘤情况;根据术前检查结果行解剖性肝段切除.精准组:除常规组进行的各项检查外,还采用吲哚菁绿排泄试验评估肝脏储备功能;通过CT对肝动脉、肝静脉和门静脉进行三维重建,并测量肝脏体积及剩余肝脏体积;术中使用低中心静脉压;采用术中超声检查明确切除范围并保护好周围脉管结构,进行精确的解剖性肝段切除.观察比较两组患者术中、术后及预后的情况.计量资料采用t检验,计数资料采用x2检验.结果 两组患者均无围手术期死亡.常规组和精准组术中全肝血流阻断时间分别为(35±25)min和(64±39)min,出血量分别为(685±524)ml和(486±360)ml,两组比较,差异有统计学意义(t=4.116,-2.033,P＜0.05);术中输血量分别为(228±398)ml和(160±330)mJ,两组比较,差异无统计学意义(t=-0.861,P＞0.05).常规组和精准组患者术后第1天ALT分别为(672±284)U/L和(344±158)U/L,第7天ALT分别为(332±161)U/L和(125±93)U/L;住院时间分别为(18±10)d和(12±6)d;术后并发症发生率分别为26%(11/43)和7%(3/42),两组比较,差异有统计学意义(t=-6.541,-7.232,-3.915,x2=5.251,P＜0.05).常规组和精准组患者术后1年肝脏肿瘤复发率分别为37%(16/43)和21%(9/42);术后1年生存率分别为88%(38/43)和93%(39/42),两组患者预后比较,差异无统计学意义(x2=0.110,0.501,P＞0.05).结论 对于结直肠癌肝转移患者,精准肝切除较常规肝切除创伤小,恢复快,更加安全、有效.

Abstract：

Objective To evaluate precise hepatectomy for liver metastases of colorectal cancer. Methods The clinical data of 85 patients with liver metastases of colorectal cancer who were admitted to the Cancer Hospital of Tianjin Medical University from October 2006 to October 2009 were retrospectively analyzed. Forty-two patients received precise hepatectomy(precise group) and 43 received routine hepatectomy (routine group). Evaluation of the hepatic and renal functions and detection of the tumors' condition were done before carrying out anatomical liver resection for patients in the routine group. Hepatic functional reserve of patients in the precise group was detected by indocyanine green excretion test. Hepatic artery, hepatic vein and portal vein were three-dimensionally reconstructed according to the data of computed tomography. The liver volume and residual liver volume of the patients were calculated. Hepatic resection was guided by intra-operative ultrasound in the precise group. Periand postoperative conditions and the results of follow-up of patients in the two groups were compared. All data were analyzed using the t test or chi-square test. Results No perioperative mortality was observed in the two groups.Time of hepatic blood flow occlusion and blood loss were (35±25)minutes and (685 ± 524) ml in the routine group, and (64±39) minutes and (486±360) ml in the precise group, respectively, with a significant difference between the two groups(t=4.116,-2.033, P＜0.05). The volumes of blood transfusion of the routine group and the precise group were (228±398) ml and (160±330)ml, respectively, with no significant difference between the two groups (t=-0.861, P＞0.05). The postoperaive levels of alanine transaminase at day 1 and day 7 were (672±284)U/L and (332±161)U/L in the routine group, and (344±158)U/L and (125 ±93) U/L in the precise group, respectively, with a significant difference between the two groups (t=-6.541,-7.232,P＜0.05). The length of hospital stay and postoperative mobidity were (18±10)days and 26% (11/43) in the routine group, and (12±6)days and 7%(3/42) in the precise group, respectively, with a significant difference between the two groups (t=- 3.915, x2=5.251, P＜0.05). The 1-year tumor recurrence rate and 1-year survival rate were 37% (16/43) and 88% (38/43) in the routine group, and 21% (9/42) and 93% (39/42) in the precise group, with no significant difference between the two groups (x2= 0.110, 0. 501, P＞0.05). Conclusion Precise hepatectomy is superior to routine hepatectomy in aspect of minimal trauma, quick recovery, efficacy and safety.     

肠乐康散剂Ⅰ期临床耐受性试验

目的观察和评价人体对肠乐康散剂的耐受性和安全性,推荐临床用药剂量.方法将30例健康受试者随机均分为0.5,1.0和2.0g3个剂量组,po,bid,共10d.试验前后作肠道菌群培养分析.结果除肠乐康散剂4.0g@d-1剂量组使食欲增加外,未见异常反应.肠道菌群培养结果表明有益菌有增加趋势.结论肠乐康散剂安全性大,耐受性好,推荐Ⅱ期临床使用剂量为po,1.0～1.5g,bid.     

益生菌治疗溃疡性结肠炎及其作用机制

日益增加的研究结果表明,肠道菌群紊乱是诱发溃疡性结肠炎(ulcerative colitis,UC)的重要原因之一.因此,应用益生菌治疗UC受到越来越多的关注.本文简述益生菌治疗UC的效果及其在信号转导途径中的作用靶标.     

益生菌在肠道黏膜损伤修复中的作用

利用益生菌调节微生态平衡、研究开发微生态制剂在世界范围内日益受到重视。微生态新药在肠道疾病的预防和治疗方面倍受关注，我国在这方面的研究工作也逐步增多。酪酸梭状芽孢杆菌（下称酪酸梭菌）防治结肠癌和凝结芽孢杆菌在治疗肠道疾病方面的作用尤其引人注意。     

对氧磷酶(Paraoxonase)的测定方法与临床意义

对氧磷酶 (Paraoxonase)是结合在高密度脂蛋白 (HDL)上的一种有机磷三酯化合物水解酶。最近研究表明 ,对氧磷酶可以保护低密度脂蛋白 (LDL)的氧化 ,其活性与基因多态性被认为是动脉粥样硬化、冠心病的独立危险因素。本文综述Paraoxonase的生化特征、基因的多态性、与动脉粥样硬化的关系及其酶活性和基因多态性的检测方法