基于波长切换技术的风热感冒颗粒UPLC指纹图谱研究

目的:建立风热感冒颗粒的UPLC指纹图谱.方法:采用UPLC法,色谱柱为Waters Acquity UPLC Hss T3 (150 mm×2.1 mm,1.8 μm),流动相为乙腈-0.01％磷酸(梯度洗脱),流速为0.5 ml· min-1,检测波长切换测定,柱温为30℃,进样量为1μl.以牛蒡苷为参照,测定15...     

UPLC法同时测定风热感冒颗粒中13种成分

目的 建立UPLC法同时测定风热感冒颗粒(板蓝根、连翘、菊花等)中(R,S)-告依春、连翘酯苷A、连翘酯苷I、连翘苷、绿原酸、新绿原酸、隐绿原酸、异绿原酸A、异绿原酸B、异绿原酸C、苦杏仁苷、牛蒡苷、牛蒡苷元的含量.方法 该药物甲醇提取液的分析采用Waters Acquity UPLC Hss T3色谱柱(150 mm...     

采用放射性骨显像评价rhBMP-2与珊瑚人工骨复合植入狗牙槽骨后的成骨效能

目的探讨应用^99Tom—MDP放射性骨显像评价重组人骨形成蛋白-2（rhBMP-2）与珊瑚人工骨在体外复合后植入狗缺损牙槽骨中后的成骨效应。方法拔除12只成年狗两侧上颌第二及第三切牙,并去除牙槽窝之间的牙槽间隔,一侧随即植入复合骨,对侧植入珊瑚骨作为对照。并于分别于植入后4、8、12周后取材,采用组织学观察、图像分析和^99Tom—MDP核素骨显像等方法比较两种类型的骨移植材料在牙槽窝中的骨修复能力。结果复合骨植入牙槽骨后,材料被逐渐降解吸收,新骨不断生成,12周后,植入材料完全被成熟的骨组织取代;图像分析结果显示不同时间复合骨组新骨形成的比值显著高于单纯珊瑚骨组（P〈0．05）;4周和8周复合骨组核素浓聚程度高于珊瑚骨组,12周两组核素浓聚程度差异不明显。结论^99Tom—MDP放射性骨显像能早期判断移植骨的成活情况及成骨效果。     

气相色谱法测定银柴颗粒中薄荷脑的含量

银柴颗粒为《国家中成药标准汇编》(中成药地方标准上升国家标准部分内科肺系分册)收载的品种[1],由忍冬藤、柴胡、芦根、枇杷叶、薄荷等药组成,具有清热、解表、止咳之功效,临床上用于治疗风热感冒,发热咳嗽。本实验以方中薄荷[2]的主要活性成分薄荷脑为测定指标,采用气相色谱法测定其含量,经方法学考察,认为本法可行,为该制剂的质量控制提供了快速、准确的测定方法。1仪器与试药Agilent6890N气相色谱仪:FID检测器,7683B自动进样器;HP—INNOWax弹性石英毛细管柱(30m×0.32mm×0.25μm);薄荷脑对照品(中国药品生物制品检定所,批号:072…     

扩张力作用下大鼠顶骨矢状缝成骨活动的定量分析

目的对扩张力作用下骨缝改建过程中成骨活动进行定量分析.方法 72只鼠龄7周左右SD大鼠,随机分为正常对照组、手术对照组、实验加力组,分别在实验第4,7,14天时各处死8只.取材制片后,采用免疫组化SP法进行内源性骨形成蛋白免疫组织化学染色,通过显微图像分析系统测量骨骨缝边缘长、骨缝组织面积、骨缝组织的面积积分光密度和骨缝组织的平均光密度.结果①加力后第4天组和第7天组实验动物的骨缝组织面积显著增加.②加力后第7天,骨缝组织中骨骨缝边缘长、骨缝组织面积积分光密度和平均光密度最高.③加力后第14天骨骨缝边缘长较加力后第7天组没有显著性差异.结论①加力后第7天骨缝纤维组织保持活跃增殖.②第7天时骨缝组织成骨活动最活跃.③第14天时沿着骨缝边缘的骨沉积和骨基质矿化持续活跃进行.     

自锁托槽矫治器摩擦力研究

随着滑动机制在固定矫治器中的普遍应用,托槽沿弓丝滑动所产生的摩擦力对正畸治疗的影响就显得尤为重要.摩擦力越大,牙齿移位时所需矫治力就越大.国外研制出一种新型矫治器--自锁托槽（self-ligating bracket）,并逐渐广泛地应用于临床.该矫治器的结构和临床特点与传统的矫治器有很大不同,减小正畸矫治的摩擦力是其最显著的特点.随着技术和设计的不断完善,这种矫治器系统大大地减小了摩擦力引起的静力学或动力学阻力,由此引起矫治技术的革新将使矫治效果更理想.本文从自锁托槽的结构、摩擦力、影响摩擦力大小的因素、临床意义等几方面进行综述.     

应用锥形束CT评估腭部种植体的植入部位

Objective To investigate the vertical bone height and the bone density of the palate for implants placement using cone beam CT(CBCT)and to provide references to the safe and stable placement of palatal implants.Methods Three-dimensional reformatting images were reconstructed with the selected CBCT scanning data of 34 patients aged 18 to 35 yeras,by means of EZ implant software.The vertical bone height was measured at 20 interesting sites of palate.Bone density was measured at 10 sites that could support 3.0mm long implants.The data of the vertical bone height and bone density were analyzed by Kmeans cluster analysis.Results According to the cluster analysis results,the 10 sites were classified into 3 clusters.There were statistical differences among these three clusters in bone height and bone density(P＜0.05).The ISD result showed that the greatest mean value of vertical bone height was obtained in cluster 2,followed by cluster 1 and cluster 3;the highest bone density was founded in cluster 3,followed by cluster 1 and cluster 2.Conclusions Evaluation of the sites for palatal implant placement with cone beam CT would be helpful in safe and stable implantation.     

正畸用粘结剂对人牙龈成纤维细胞环氧化酶-2表达的影响

目的通过检测正畸用带环粘结材料与人牙龈成纤维细胞共培养后环氧化酶-2表达水平的变化,评估正畸用带环粘结材料对牙周组织的刺激作用。方法实验采用两种常用的正畸粘结剂材料,分成6个实验组,树脂类粘结剂的糊剂组、树脂类粘结剂的底剂组、树脂类粘结剂的聚合后产物组、玻璃离子粘结剂的粉剂组、玻璃离子粘结剂液体组、玻璃离子粘结剂混合后聚合产物组。上述6组实验材料分别置于人牙龈成纤维细胞培养液中,与人牙龈成纤维细胞一起培养。对照组分别设阴性对照和阳性对照组。实验组在培养1、3、6、12及24 h时收集样本,对照组在培养24 h时收集样本,测量环氧化酶-2 mRNA的表达水平。结果人牙龈成纤维细胞环氧化酶-2 mRNA的表达水平在培养6 h时达到高峰,其中以树脂类粘结剂的底剂和玻璃离子类粘结剂的粉剂组升高明显。结论正畸用粘结剂通过升高人牙龈成纤维细胞环氧化酶-2 mRNA的表达水平造成牙周组织的损害。     

下颌角咬肌肥大的外科矫治

下颌角骨质异常增生及咬肌良性肥大表现为方形脸，是并不罕见的发育畸形。自１９８７～１９９４年共治疗１２例，获得了良好的效果，手术方法采用口腔内进路完成肥大下颌角及咬肌的切除。强调只要手术操作仔细，术后有效加压包扎及冰敷，并发症可以避免。     

应用锥形束CT评估腭部种植体的植入部位

Objective To investigate the vertical bone height and the bone density of the palate for implants placement using cone beam CT(CBCT)and to provide references to the safe and stable placement of palatal implants.Methods Three-dimensional reformatting images were reconstructed with the selected CBCT scanning data of 34 patients aged 18 to 35 yeras,by means of EZ implant software.The vertical bone height was measured at 20 interesting sites of palate.Bone density was measured at 10 sites that could support 3.0mm long implants.The data of the vertical bone height and bone density were analyzed by Kmeans cluster analysis.Results According to the cluster analysis results,the 10 sites were classified into 3 clusters.There were statistical differences among these three clusters in bone height and bone density(P＜0.05).The ISD result showed that the greatest mean value of vertical bone height was obtained in cluster 2,followed by cluster 1 and cluster 3;the highest bone density was founded in cluster 3,followed by cluster 1 and cluster 2.Conclusions Evaluation of the sites for palatal implant placement with cone beam CT would be helpful in safe and stable implantation.