RP-HPLC法测定五苓散中麦角甾醇和桂皮醛的含量

目的建立五苓散中麦角甾醇和桂皮醛的含量测定方法。方法五苓散样品经甲醇超声提取后,用HPLC法测定。色谱柱为HypersllC18（250mm×4.6mm,5μm）,流动相为甲醇-水梯度洗脱,流速为1mL.min-1,检测波长为283nm,柱温为30℃。结果麦角甾醇在0.0437~1.3098mg.mL-1浓度范围内线性关系良好（r=0.9991）,平均回收率为102.9%,RSD为1.5%（n=6）;桂皮醛在0。0062~0.1872mg.mL-1浓度范围内线性关系良好（r=1.0000）,平均回收率为97.8%,RSD为1.9%（n=6）。结论该方法简便、准确、可靠,可用于五苓散中麦角甾醇和桂皮醛的同时含量测定。     

一个中国Gilbert综合征家系的临床和遗传学特征分析

Objective To perform clinical and genetic pedigree analyses of a Chinese male patient with Gilbert's syndrome and his relatives. Methods Blood sample were collected from the proband and his relatives by liver function test, etiological examination and genetic analysis to exclude other related diseases. The phenobarbital-responsive enhancer module (PBREM) , TATA box and common mutation sites in exons of UGT1A1 gene were amplified by polymerase chain reaction (PCR) and the products screened by direct DNA sequencing. Results c. -3279T ＞ G in PBREM, TA insertion in TATA box and Gly71Arg were observed in this family. A linkage disequilibrium is also noted between c. -3279T ＞ G and TA insertion. In four affected members, three are heterozygotes and one is homozygote. The correlation between genotype and phenotype with a high serum level of unconjugated bilirubin was confirmed. Conclusion c. -3279T ＞ G in PBREM, TA insertion in TATA box and Gly71 Arg are essential for the pathogenesis of Gilbert's syndrome in this Chinese family. Gilbert's syndrome in this family is inherited in an autosomal recessive manner.     

角膜塑型术的相对安全性研究

目的 探讨角膜型术中出现并发症的机率、原因及处理治疗方法，从而评估角膜塑型术在青少年近视治疗过程中的相对安全性问题。方法 对233例444眼青少年近视患者进行了规范的DYNALENS角膜塑型术治疗，在近3年的随访检查过程中，及时做出对症处理。结果 治疗过程中出现角膜染色282眼（63．4％），角膜水肿10眼（2．3％），角膜溃疡5眼（1．1％），斜向散光103眼（23．2％），明显角膜变形（重影）34眼（7．6％），过敏性眼炎8眼（1．8％）。经过对症治疗，取得明显的疗效，总治愈率达95．2％。结论 运用规范的配验技术，严格的随记计划，角膜地形图的追踪分析，角膜塑型术是一种相对安全的方法。     

高透氧性硬性角膜接触镜治疗屈光参差性弱视的疗效评估

目的评估高透氧性硬性角膜接触镜治疗屈光参差性弱视的疗效。方法将符合屈光参差性弱视诊断的53例患者分为两组,A组26例配戴高透氧性角膜接触镜,B组27例配戴框架眼镜并同时给予遮盖、精细目力等弱视治疗,对比两组患者随访1个月、6个月、1年、2年后的疗效。结果在随访1个月、6个月、1年、2年后,A组患者的弱视眼矫正视力均明显提高,有效率显著高于B组患者,两组疗效差异具有统计学意义（P〈0.05）,且A组患者均能满意接受配戴RGP;1月后100%均感配戴舒适,在随访中未发现角膜上皮脱落、巨乳头性结膜炎、感染、眼部痛、痒等症状及眼镜护理方面的问题。结论高透气性硬性角膜接触镜基于其优质的像质和较小的像差,能安全、有效地治疗屈光参差性弱视,与其他治疗方法相比具有其独特的优越性。