Primary culture of retinal Müller cells in adult rats assisted by the peritoneal exudative cells

Objective To investigate the effect of peritoneal exudative cells as feeder cells on growth from adult rats, which were identified with specifically biological marker of maerophage (CD68). The by enzyme digestion method, and identified by GFAP and vimentin immunocytochemicaUy. As the feeder cells. Results Over ninety-five percent of rat peritoneal exudative cells were macrophage, which have a flask and grew fast, with large applanate cell bodies. The third-generation cells grew slowly. After cocuhured with feeder cells, the Moiler cells showed more rapid growth rate with more cells in S and G2/M phase(S phase, t=4. 172, P<0.001; G2/M phase, t=3. 562, P<0.01)and less apoptotic rate (t =3. 804, P<0. 01). The growing cycle was cut down from 25-30 days to 18-22 days for the first-generation cells, from 10-15 days to 7-10 days for the second-generation cells. Conclusion It is an effective method to use the peritoneal exudative cells as feeder cells cocuhured with primary culture of retinal Müller cells,which can shorten the culture period of Müller cells in adult rats.     

转化生长因子β在角膜屈光手术中作用的研究进展

转化生长因子刘双珍（transforming growth factor beta,TGF-β）作为一种具有双向性作用的细胞因子，在多种细胞中广泛表达。由于角膜屈光手术的广泛开展，TGF-β在角膜中的表达及作用备受关注。本研究叙述了TGF-β的结构、生物学作用、对角膜创伤修复的调节及其在角膜屈光手术中的作用的研究进展，     

CT对眼部占位病变诊断的初步分析

我院自1985年开始对临床诊断为眼部占位病的45例(47眼)患者进行了CT 检查,其中28例(30眼)经过手术治疗。现将其结果初步分析如下。临床资料经临床诊断为眼部占位病者45例的CT检查结果(表),CT 检查的阳性结果为96.66%。     

视网膜母细胞瘤眼球摘除后健眼失明

陈××男3岁住院号299812 自1985年7月,发现左眼瞳孔区反黄光,当时由于患儿无任何不适,未引起重视,黄光反射逐渐明显。近半月来,左眼发红、畏光、流泪。于1986年9月30日入院。全身检查未见异常。右眼正常。遮盖右眼,左眼不能寻见物体。球结膜混合性充血,下方球结膜水肿,瘀血,角膜呈云雾状,前房极浅,可见黄绿色、颗粒状、类似前房积脓约2mm。瞳孔约7mm,对光反射消失。虹膜纹理不     

共同性及麻痹性斜视眼外肌显微结构的研究

目的　观察共同性斜视、麻痹性斜视及正常人眼外肌显微、超微结构的异同。方法　共同性斜视弱侧肌 (作用力与眼位偏斜相反 )、麻痹性斜视拮抗肌各 10例 ,分别行光、电镜观察 ,并计算单位面积中肌纤维数量 ,正常人眼外肌 10例作为对照组。结果　共同性斜视组肌纤维排列紊乱 ,萎缩变性 ,胶原组织增生 ,随病程延长而显著 ;麻痹性斜视组肌纤维轻度肥大 ,排列稍紊乱 ,未见肌组织溶解坏死及胶原组织增生。共同性斜视组肌小节结构破坏 ,M线、Z线模糊 ,线粒体空泡样变 ,有异常颗粒 ;麻痹性斜视组肌小节结构完整 ,M线、Z线可见 ,肌细胞内线粒体增多并堆积。两斜视组肌纤维含量分别为 :13 3 3± 1 5 2 /12 60 0 μm2 、 18 93± 2 3 1/12 60 0 μm2 ,共同性斜视组肌纤维含量显著少于正常人组 (19 7± 1 5 0 /2 60 0 μm2 )和麻痹性斜视组 (P <0 0 1) ,而麻痹性斜视组与正常人组无明显差别。结论　共同性斜视弱侧肌出现萎缩、胶原组织增生 ,随病程延长而加重 ,提示共同性斜视在保守治疗无效时应尽早手术。麻痹性斜视拮抗肌形态学改变与其功能亢进相一致 ,主要表现为肌纤维轻度肥大和胞浆中线粒体增生并异常堆积。     

血管活性肠肽及其在眼科学的研究进展

血管活性肠肽作为神经肽递质,广泛分布于全身和眼球各器官组织中,发挥广泛而重要的信息传递和生理调控功能。本文就血管活性肠肽及其受体的结构、分布与功能,特别是对眼球生理功能及视觉发育调控的研究现状作一综述。     

眼眶肿瘤手术并发症及其影响因素

目的:通过对215例眼眶肿瘤手术并发症分析,探讨手术并发症的发生规律,明确主要的影响因素。方法:根据已经获得的临床资料,分析与眼眶手术并发症密切相关的因素,然后进行统计学处理。结果:45例发生了54种不同类型的并发症(25.12％)。并发症发生最多的是术后眼球运动障碍(31.48％),然后依次为上睑下垂(22.22％)、球后出血(18.51％)、视力障碍(12.96％)、瞳孔散大(12.96％)、硬脑膜损伤(1.85％)。结论:眼眶肿瘤手术并发症的发生主要与肿瘤发生的部位(P=0.027)、肿瘤的性质(P=0.045)、肿瘤累及范围、肿瘤边界是否清晰有关(P<0.005),也与手术入路的选择有密切联系(P<0.005)。     

Primary culture of retinal Müller cells in adult rats assisted by the peritoneal exudative cells

Objective To investigate the effect of peritoneal exudative cells as feeder cells on growth from adult rats, which were identified with specifically biological marker of maerophage (CD68). The by enzyme digestion method, and identified by GFAP and vimentin immunocytochemicaUy. As the feeder cells. Results Over ninety-five percent of rat peritoneal exudative cells were macrophage, which have a flask and grew fast, with large applanate cell bodies. The third-generation cells grew slowly. After cocuhured with feeder cells, the Moiler cells showed more rapid growth rate with more cells in S and G2/M phase(S phase, t=4. 172, P<0.001; G2/M phase, t=3. 562, P<0.01)and less apoptotic rate (t =3. 804, P<0. 01). The growing cycle was cut down from 25-30 days to 18-22 days for the first-generation cells, from 10-15 days to 7-10 days for the second-generation cells. Conclusion It is an effective method to use the peritoneal exudative cells as feeder cells cocuhured with primary culture of retinal Müller cells,which can shorten the culture period of Müller cells in adult rats.     

低氧诱导因子-1α干扰RNA对血管内皮生长因子mRNA表达的抑制作用

目的 探讨低氧诱导因子-1α（hypoxia inducible factor-1α．HIF-1α）特异性小片段干扰RNA表达载体pSUPER^H1-SiHIF1α对低氧诱导因子-1α和血管内皮生长因子（vascular endothelial growth factor．VEGF）mRNA表达的抑制作用。方法 构建HIF-1α特异性小片段干扰RNA表达载体pSUPER^H1-SiHIF1α通过脂质体Lipofectamine2000分别介导pSUPER^H1-SiHIF1α转染低氧状态下培养的人低分化鼻咽鳞癌细胞（nasopharyngeal carcinoma cell line，CNE2），对照组转染pSUPER空载体．采用半定量RT—PCR方法检测HIF-1αmRNA和VEGF mRNA的表达。结果 转染DSUPER^H1-SiHIF1α的CNE2细胞中HIF-1α mRNA和VEGF mRNA的表达较对照组分别降低79．4％和65．7％。结论 HIF-1α特异性小片段干扰RNA表达载体pSUPER^H1-SiHIF1α能明显抑制HIF-1α mRNA和VEGF mRNA的表达。     

显微吻合修复87例泪小管断裂

目的探讨显微镜下修复泪小管断裂的方法及其手术效果。方法回顾性分析应用显微外科技术,行泪小管吻合术的87例泪小管断裂患者随访2～18个月的病例资料。结果87例中治愈63例,治愈率72.41%。锐器伤的治愈率最高,挫裂伤次之,车祸及爆炸伤最低（P〈0.05）;伤后36h内手术的治愈率高于36h后（P〈0.05）;不同吻合方式之间的治愈率比较,差异无统计学意义;用硬膜外导管、输尿管导管及硅胶管作为义管的疗效相似。结论泪小管显微吻合术是治疗泪小管断裂的有效方法,致伤原因和伤后至手术的时间影响其手术效果。