急性淋菌性腹膜炎28例诊治体会

急性淋菌性腹膜炎是由淋病双球菌引起的急性腹膜感染。现将诊治体会总结如下。     

高效液相色谱法测定人血浆中头孢替坦二钠含量的不确定度

[摘要] 目的：HPLC法测定人血浆中头孢替坦二钠含量不确定度的评定。方法：对测定过程中不确定度的来源进行分析评定,包括测定重复性、称量、标准溶液的配制、含药血浆的配制、血浆样本的处理、仪器、标准曲线拟合等,计算合成不确定度和扩展不确定度。结果：人血浆中低(1.2 μg?mL-1)、中(27.0 μg?mL-1)、高(270.0 μg?mL-1) 3个浓度的扩展不确定度分别为0.12,2.50,28.65 μg?mL-1(k=2,P=95%)。结论：HPLC法测定人血浆中头孢替坦二钠含量的不确定度主要由血浆样本配制和处理,标准曲线拟合（低浓度）及仪器允差引入。     

A simple and sensitive liquid chromatographic technique for the determination of cefotetan disodium in human plasma and its application in a pharmacokinetic study

A simple and sensitive liquid chromatographic method was developed for quantification of cefotetan disodium (CTT),a semi-synthetic cephamycin antibiotic,in human plasma.CTT and the internal standard chloramphenicol were extracted from plasma by a simple one-step protein precipitation with 35% (v/v) perchloric acid.Separation was carried out on a reverse-phase C18 column with a mobile phase of acetonitile-water containing 0.5% (v/v) phosphoric acids (20:80,v/v) at a flow rate of 1.0 mL/min.The column effluent was monitored by UV detection at 300 nm.The column temperature was maintained at 40°C.This method demonstrated good linearity in the range of 0.525-300.0 μg/mL,with correlation coefficients greater than 0.99.The limit of quantification (LOQ) was 0.525 μg/mL in human plasma.Intra-and inter-day precisions were less than 6.63% in terms of relative standard deviation (RSD).The accuracy,when expressed by the bias,ranged from 0.57% to 4.04%.The mean extraction recovery of CTT was higher than 40.94%.The method was found to be precise,accurate,and specific for CTT quantitative analysis,and was successfully applied for a pharmacokinetic study of CTT after a single intravenous dose of 1.0 g of CTT in healthy Chinese subjects.     

RAC1 基因多态性对Rac1-GTP 蛋白表达水平的影响

目的:考察RAC1 基因多态性与Rac1-GTP 蛋白表达水平的相关性。方法:选择182 例健康汉族志愿者,经TaqMan-MGB 探针等位基因分型技术测定RAC1 基因中的4 个标签单核苷酸多态性(tag single nucleotide polymorphism,tag-SNPs)位点的基因型,采用酶联免疫吸附法测定其血浆中的Rac1-GTP 蛋白表达水平。分析Rac1-GTP 蛋白表达水平的分布特点;对RAC1 基因4 个tag-SNPs 不同基因型间Rac1-GTP 蛋白表达水平进行比较。结果:Rac1-GTP 蛋白表达水平在健康汉族人群中呈现正偏态分布,女性中的表达水平高于男性(P<0.05);随着年龄的增加有降低的趋势,但差异无统计学意义(P>0.05)。Rac1-GTP 蛋白表达水平在位点rs702482 和rs10951982 基因型间存在显著性差异(P<0.05),但在位点rs702483 和rs6954996 基因型间无统计学差异(P>0.05)。结论:在健康汉族人群中RAC1 基因多态性存在影响Rac1-GTP 蛋白表达水平的SNP 位点。