人组织因子途径抑制物基因在兔颈动脉损伤血栓形成中的作用

Objective To observe the effects of tissue factor pathway inhibitor(TFPI) on thrombosis formation in rabbit carotid arteries after ballon injury. Methods Fouty rabbits with the weight 2.5-3.0 kg were respectively divided into 4 groups, Ad-TFPI, Ad-LacZ, PBS and normal control groups. The normal control group was not given any treatment and other 3 groups were given 0.2 ml Ad-TFPI, Ad-LacZ or PBS reproduced by the Dispatch catheter respectively after the PTCA balloon iniury on the right carotid arteries. Ten days after gene transfer the repeated balloon injury was performed in the 3 groups, and the first balloon injury was performed in the normal control group by the same method. The carotid blood flow was recovered immediately after the injury. Thirty minutes later all the animals were sacrificed. The injured carotid arteries and one part of contralateral normal artery were cut down, scissored along the long axis, flattened and fixed in the 2% glutaral. The platelet aggregation and thrombosis formation on the luminal surfaces was observed under electron microscope. Results The electron microscope results showed that the vascular endothelial cell structure was integrated and lined up in order in the nomal artery which had no any injury. After the balloon injury in the normal control group, the structure of the endothelial cell was disintegrated, and there was some platelet aggregation but no fibrosis formation. A large amount of platelet aggregated but no fibrosis formed in Ad-TFPI group after the repeated balloon injury. A large amount of fibrosis formed and red cells piled up in the Ad-LacZ and PBS group. The positive rate of thrombosis formation among groups had siginificant differences(χ2=14.95, P<0.01). The positive rate in Ad-TFPI group(20%) was lower than that in Ad-LacZ group(80%, χ2=7.20, P<0.01) and PBS group(70%, χ2=5.05, P<0.05), but was higher than that in the normal control group(10%, χ2=0.39, P>0.05). The positive rate in Ad-LacZ group(80%) was higher than in the normal control group(10%, χ2=9.90, P<0.01) and in the PBS group(70%, χ2=0.27, P> 0.05). The positive rate in PBS group(70%) was higher than that in the normal control group(10%, χ2=7.50, P< 0.01). Conclusions The repeated balloon injury method can cause a large amount of fibrosis formation in the rabbit carotid. TFPI gene inhibits thrombosis formation in balloon-injured rabbit carotid arteries.     

痰热清治疗失代偿肝硬化并急性肺炎的疗效观察

失代偿性肝硬化是各种慢性肝病的晚期阶段，主要表现为肝细胞功能衰竭，机体免疫防御功能低下，易发生各种感染，肝硬化患者为急性肺炎的易感人群之一，而急性肺炎起病急，临床症状多为发热、咳嗽、咳黄白色或黄色粘痰和胸痛等症状，因此临床选择具清热、解毒、化痰多种药效为一体而对肝脏没有损害或基本没有损害的药物具有十分重要的意义。为此，我科采用痰热清注射液治疗失代偿肝硬化合并急性肺炎，并设对照组加以比较，由此探讨痰热清的疗效及对肝脏的影响。     

木防己汤治疗慢性充血性心力衰竭30例

临床资料　本组 6 0例 ,男 41例 ,女 1 9例 ;年龄 1 8～ 85岁 ,平均 5 0 .3岁 ;心衰史 1～ 1 6年 ,平均 5 .1年。 6 0例中风湿性心脏 1 6例 ,冠心病 1 5例 ,高血压心脏病 1 3例 ,扩张性心肌病 1 6例。临床表现以心悸 ,气急 ,动则喘甚 ,端坐呼吸 ,不能平卧 ,双下肢浮肿 ,舌淡胖、苔薄白 ,脉沉细数或结代等心气阳虚 ,水气上逆之证 ,不符合本证型者剔除。 6 0例随机分为治疗组 3 0例 ,对照组 3 0例 ,两组年龄、性别、病程等经统计学处理无显著差异 ,具有可比性。　　治疗方法　治疗方法采用木防己汤 ,药物组成 :人参 6 g,防己 1 5 g,桂枝、生石…     

郎罕细胞组织细胞增多症

郎罕细胞首先是由ＰａｕｌＬａｎｇｅｒｈａｎｓ于１８６８年报道的一种组织细胞亚型，正常发现于皮肤的表皮中，偶尔也可在支气管、口腔粘膜、食管、胸腺、结肠下段等组织中发现。１９５３年，Ｌｉｃｈｔｅｎｓｔｅｉｎ等发现“嗜酸细胞肉芽肿”、“韩—薛—柯病”、“勒...     

57例新生儿窒息后脑损伤阴离子间隙的分析

窒息新生儿常因缺氧出现脑损伤 ,且多伴有电解质、酸碱平衡紊乱。内环境稳定是维持脏器功能正常的一个重要因素。及时准确判断酸碱失衡的性质并给予纠正 ,对降低危重新生儿的死亡率、提高生存质量有重要意义。本文对 5 7例出生窒息并经复苏处理后的新生儿作动脉血气、血电解质、头颅ＣＴ检查 ,计算阴离子间隙 (ＡＧ)值 ,探讨窒息后脑损伤新生儿ＡＧ值的变化及对酸碱失衡的影响。1　资料与方法1 1　临床资料　 2 0 0 0年 10月～ 2 0 0 1年 12月我院收治生后 2 4ｈ以内的新生儿 ,出生时有窒息史 ,经头颅ＣＴ证实有脑损伤者 5 7例。其中男 3 6…     

公立医院社会功能的定量评价

该文对不同级别公立医院的社会功能指标及其在1985－2000年之间的变化情况进行了测算，讨论、分析了这些指标的影响因素，发现基层医院比三级医院具有较强的社会功能，在转轨时期公立医院社会功能有下降趋势。     

四点法治疗重症肩周炎的体会

四点法治疗重症肩周炎的体会侯重庆＊自１９８９年４月至今，笔者采用“四点法”药物注射治疗重症肩周炎，报道如下。临床资料本组共６７例，治疗６９人次。其中男５２例，５４人次，年龄４３～６０岁，平均５０±２岁。女１５例，年龄２７～５８岁，平均４７±３岁。病史...     

女性的“性福”谁来呵护

一位有近二十年从医经验的妇科医生告诉我,目前,女性的生殖健康观念亟需加强。你瞧,看妇科病的女同志,不仅没人陪,还得躲躲闪闪的。可来做妊娠期检查的大都有“保镖”在外面等候。这位医生还认真地说,从她接待过的病例看,不少女性在最为隐秘的夫妻生活,也就是性问题上同样存在着被丈夫忽略的烦恼。在走访了几位已婚女性之后,我发现,这位医生的话至少在一定程度上反映了一种事实——女性的“性福”亟待关注,尤其是来自自己另一半的关注。     

利用化验指导抗艾滋病毒疗法

艾滋病在潜伏期无症状。有的国家和地区曾带有戏谑韵味的称之谓“爱之病”,这在科学的含义上虽不确切,却也道出了某些真谛。病情一旦发作,常常缠绵悱侧,或极为凶险。例如该病毒引起免疫力极度低下,易继发感染;当合并卡氏肺囊虫性肺炎时,从首次发病起即使用现代疗法也只能维持18—24个月。发生与艾滋病有关的淋巴瘤后,其平均生存期为12个月;在80年代早期流行时为8个月。经过十余年的技术进步只不过多延长了4个月。