成人睾丸组织在裸鼠体内的存活情况研究

目的以成人睾丸组织为供体,免疫缺陷小鼠为受体,研究成人睾丸组织中生精细胞异种移植后的存活及变化情况。方法将成人正常睾丸组织植入去势裸鼠背部,于移植后110d取出移植物,进行组织形态学观察。结果在一例移植前显示非正常生精上皮结构标本的移植物中,大多数生精小管中只含有Sertoli细胞,而另一例移植前显示出正常生精上皮结构的标本,在移植到裸鼠背部110d后,仍然观察到圆形和长形精子细胞存在。结论将成人睾丸组织移植到免疫缺陷小鼠体内,110d后仍观察到有生精细胞的存活。     

两种抗生素治疗呼吸道感染的对比观察

呼吸道感染是门诊常见疾病，治疗的关键在于如何恰当的运用经验，制订适合患者实际状况的治疗方案。抗生素作为主要治疗手段必须基于预测疗效、清除细菌、防止产生耐药性等原则。阿奇霉素对临床上多种常见致病菌有抗菌作用；头孢他啶是第三代头孢菌素，对大多数革兰阳性菌、阴性菌均有抗菌活性。在本院各种呼吸道感染住院患者中，分别随机抽取应用阿奇霉素、头孢他啶为主要治疗手段的患者共81例，研究、评价阿奇霉素、头孢他啶治疗呼吸道感染的疗效和安全性。     

人细小病毒B19感染与小儿特发性血小板减少性紫癜

目的探讨小儿特发性血小板减少性紫癜(ITP)与人细小病毒B19(HPVB19)感染的关系.方法用ELISA技术对102例ITP患儿进行人HPVB19特异性IgM的检测.结果 102例ITP患儿中HPVB19-IgM阳性41例(40.20%),30例正常对照HPVB19-IgM阳性2例(6.67%),ITP组HPVB19感染率明显高于正常对照组,两组比较有显著性差异(P＜0.05).结论人细小病毒B19(HPVB19)与小儿 ITP 的发生密切相关.     

浅谈人口老龄化引起的抗生素用药不安全因素

目前人口老龄化日益明显。老年人在生理、心理等方面处于衰老退化的状态,这种变化可影响药物的吸收、分布、代谢和排泄,同时亦影响药物的效应和引起不良反应的发生。老年人是一个特殊的群体,全社会应关注老年人的用药问题,特别是抗生素的用药问题。通过对此问题的分析,医师和药师要认真履行各自的职责,在充分认识老年患病特点的基础上,采取安全对策,注意合理选择药物。     

Conversion of mononuclear cells from human umbilical cord blood into hepatocyte-like cells

Objective: To evaluate the differentiation of human umbilical cord blood cells into hepatocyte-like cells. Methods: Mononuclear cells (MNCs) derived from human umbilical cord blood were isolated using Ficoll. The experiment was derived into 3 categories:(1) MNCs co-cultured with 50 mg minced liver tissue separated by a trans-well membrane and then collected at 0 h,24 h,48 h and 72 h; (2) MNCs cultured along supplemented with 100 ml/L FBS. 100μ/ml penicillin, 100μg/ml streptomycin. 4. 7μg/ml linoleic acid, 1×ITS, 10-4 mol/L L-Ascorbic acid 2-P and a combination of FGF4 (100 ng/ml) and HGF (20 ng/mL). Cells were then collected at 0 d and 16 d to examine the expression profile of hepatocyte correlating markers;(3) 0. 2-0. 3 ml of MNCs with a cell density of 2×107/ml were transplanted into prepared recipient mice [n = 12, injected with 0. 4 ml/kg (20%) CCl4 and 150 ng/kg 5-fluorouracil (5-Fu) prior the transplant 24 h and 48 h, respectively] via injection through tail vein. Mice were sacrificed 4 weeks after transplantation. The hepatocyte correlating mRNAs and proteins were determined by RT-PCR, immunohistochemical analysis and immunoflurence technique. Results: (1) After 72 h. a number of glycogen positive stained cells were observed with MNCs co-cultured with damaged mouse liver tissues. The expression of hepatocyte markers, human albumin (ALB),α-fetal protein (AFP) and human GATA4 mRNA and proteins were detected by RT-PCR and immunohistochemistry as well. For the confirmation, the DNA sequencing of PCR products was performed. In control groups, MNCs co-cultured with normal mouse hepatocytes or MNCs cultured alone, all markers remained negative. (2) In growth factor supplemented culture system, MNCs developed into larger volume with richer cytoplasm and binucleation after 16 d. Positive expression of ALB, AFP, CK18 and CK19 mRNA were detected with RT-PCR, and ALB positive staining was observed by immunocytochemistry as well. In contrast. MNCs cultured without exogenous growth factors scarcely attached to the culture dish and ALB mRNA was not detected. (3) In transplantation experiment, both of ALB and AFP mRNA were detected by RT-PCR and HSA, PCNA and ALB positive staining were observed in the livers of recipient mice by immunocytochemistry. Conclusion: MNCs from human umbilical cord blood could convert into hepatocyte-like cells in 3 different ways, indicating their potential use in the clinic applications for the treatment of human liver diseases.     

间充质干细胞与再生障碍性贫血免疫紊乱关系的研究

再生障碍性贫血(再障)的发病机制主要涉及免疫功能紊乱、造血微环境异常和造血干/祖细胞破坏,而其中免疫功能紊乱起到非常重要的作用.间充质干细胞具有造血支持作用,也具有免疫调节作用.将再障的间充质干细胞的免疫调节作用与免疫功能紊乱发病机制联系起来,将为再障进一步的研究提供一个新的方向.     

ICE中间件技术在医疗数据集成平台中的应用

现代医院的数字化建设从深度和广度上都有了新的发展，医疗数据集成平台也应运而生。这种特殊的信息系统可以将其他各种不同构架设计、不同实现手段和不同开发环境的信息系统结合起来，按照一定的规范进行数据交互，并结合业务流程实现业务的跨系统整合。文章从医院数据集成平台的设想和构建实际出发，提出了数据集成平台设计理念、构架模块方面的理论设想，详细讨论了ICE中间件技术的应用，并将在实际建设中加以进一步验证和落实：     

足叶乙甙-铂类联合方案用于耐药性滋养细胞疾病的治疗

为进一步探讨足叶乙甙-铂类(Etoposide-Platin,EP)联合化疗对耐药性滋养细胞疾病的治疗作用及其毒性,对1984～1992年间7例化疗耐药的恶性妊娠滋养细胞疾病(gestational trophoblastic disease,GTD)接受EP联合化疗进行回顾性分析。病例均经病理诊断为绒毛膜癌或临床诊断妊娠后继发恶性GTD,伴hCG上升;开始化疗时均有转移灶的影像学诊断;在EP化疗前已接受过平均6程的其它药物     

苯酚-硫酸法测定河南丝瓜络多糖的含量

目的测定河南产丝瓜络多糖的含量,探讨其药用价值和开发前景。方法采用苯酚-硫酸法,以葡萄糖(105℃干燥至恒重)为对照品测定河南丝瓜络中多糖的含量。结果在6~91μg.mL-1的范围内,样品的浓度与吸光度有良好的线性关系,线性回归方程A=8.995 8 c-0.007 8(r=0.999 7),加标回收率101.8%(n=5,相对标准偏差=1.59%),河南丝瓜络多糖的平均含量为7.66%。结论河南产丝瓜络的多糖含量高于广东、山西和浙江等产地,具有较高的药用价值和开发前景。     

防治药源性疾病是医药工作者的重要职责

药源性疾病已对人类的健康构成威胁，必须引起关注。要积极开展对药源性疾病这个新的医药学科的学习，从诊断入手，加强药源性疾病的预防和治疗工作。这关系到患者的利益，也是我们医药工作者的重要职责。