中药复方重肌灵免疫调节作用及其机制的实验研究

目的：探讨中药复方重肌灵免疫调节作用及其机理。方法：建立Lewis大鼠EAMG模型。采用3H—TdR法检测重肌灵对Cona、脂多糖(LPS)诱导正常BALB／C小鼠脾淋巴细胞增殖反应的影响，及重肌灵对ConA、N2AchR诱导EAMG大鼠淋巴结细胞增殖反应的影响。ELISA法测定重肌灵对EAMG大鼠血清中N2AchRab滴度及血清中IFN—γ水平的影响。结果：重肌灵对ConA、LPS诱导正常ALB／C小鼠脾淋巴细胞增殖反应有明显的促进作用(P＜0.05)；对ConA诱导EAMG大鼠淋巴结细胞增殖反应有明显的促进作用(P＜0．01)，而对N2AchR诱导EAMG大鼠淋巴结细胞增殖反应有明显抑制作用(P＜0．01)。重肌灵对EAMG大鼠血清中N2AchR滴度及血清IFN—γ水平有明显下调作用(P＜0．01)。结论：重肌灵防治EAMG不是通过免疫抑制，而是利用它的免疫调节作用。机理可能是通过下调IFN－γ水平，而抑制N2AchR抗原诱导特异性免疫反应，而起到治疗作用。     

微波诱导视网膜神经细胞凋亡及硒的保护作用

目的 观察微波诱导视网膜神经细胞凋亡及硒的保护作用。方法 体外培养视网膜神经细胞,按微波辐照强度分为４组辐照１ｈ,选３０ｍＷ．ｃｍ＾－２组为防护组,在培养液中加入Ｎａ２ＳｅＯ３后辐照,照后测培养液温度,细胞存活率,激光共聚焦显微镜观察细胞凋亡并进行荧光强度的定量分析。结果 培养液温度,细胞凋亡数量及荧光强度 随微波辐照强度增大而增加,加Ｎａ２ＳｅＯ３后辐照可使凋亡细胞数量减少,荧光强度减弱。结论微     

Analysis of density and epitopes of D antigen on the surface of erythrocytes from DEL phenotypic individuals carrying the RHD1227A allele

Background

The characteristics of the D antigen are important as they influence the immunogenicity of D variant cells. Several studies on antigenic sites have been reported in normal D positive, weak D and partial D cases, including a comprehensive analysis of DEL types in Caucasians. The aim of this study was to assess D antigen density and epitopes on the erythrocyte surface of Asian type DEL phenotypic individuals carrying the RHD1227A allele in the Chinese population.

Materials and methods

A total of 154 DEL phenotypic individuals carrying the RHD1227A allele were identified through adsorption and elution tests and polymerase chain reaction analysis with sequence-specific primers in the Chinese population. D antigen density on the erythrocyte surface of these individuals was detected using a flow cytometric method. An erythrocyte sample with known D antigen density was used as a standard. Blood samples from D-negative and D-positive individuals were used as controls. In addition, D antigen epitopes on the erythrocyte surface of DEL individuals carrying the RHD1227A allele were investigated with 18 monoclonal anti-D antibodies specific for different D antigen epitopes.

Results

The means of the median fluorescence intensity of D antigen on the erythrocyte membrane surface of D-negative, D-positive and DEL individuals were 2.14±0.25, 193.61±11.43 and 2.45±0.82, respectively. The DEL samples were estimated to have approximately 22 D antigens per cell. The samples from all 154 DEL individuals reacted positively with 18 monoclonal anti-D antibodies specific for different D antigen epitopes.

Discussion

In this study, D antigen density on the erythrocyte surface of DEL individuals carrying the RHD1227A allele was extremely low, there being only very few antigenic molecules per cell, but the D antigen epitopes were grossly complete.     

组织芯片技术检测PTEN、P53及hTERT在I型子宫内膜癌中的表达特征

目的:应用组织芯片技术检测磷酸酶及张力蛋白同源基因(PTEN)、P53及人端粒酶反转录酶(hTERT)在Ⅰ型子宫内膜癌中的表达,为Ⅰ型子宫内膜癌的早期诊断和治疗提供更可靠的理论依据。方法:应用组织芯片技术和免疫组化技术检测Ⅰ型子宫内膜癌和正常子宫内膜组织中P53、PTEN及hTERT的表达,并与传统免疫组化研究结果进行对比分析。结果:组织芯片技术的研究结果与传统免疫组化结果具有较好的一致性(Kappa值>0.40,P<0.05),研究结果可靠性较强。PTEN在Ⅰ型子宫内膜癌中的阳性表达率明显低于正常子宫内膜组织;hTERT和P53在Ⅰ型子宫内膜癌中的阳性表达均高于正常子宫内膜组织。结论:组织芯片技术可作为一种有效的方法应用于Ⅰ型子宫内膜癌研究,PTEN的表达缺失、P53及hTERT的阳性表达与Ⅰ型子宫内膜癌的形成和发展有关,三者均可作为Ⅰ型子宫内膜癌诊断及判定预后的标记物。     

C-反应蛋白水平对急性缺血性脑卒中预后影响的系统评价

目的 应用Meta分析,评价C-反应蛋白(C-reactive protein,CRP)水平对急性缺血性脑卒中预后的影响.方法 计算机检索PubMed、MEDLINE(1991-2006)、中国学术期刊全文数据库(1979-2006)等.搜集CRP水平对急性缺血性脑卒中预后影响非随机对照研究,逐篇评价纳入文献的质量,采用RevMan4.2版软件进行Meta分析.结果 共纳入7篇文献(外文4篇,中文3篇),涉及841例患者.在急性缺血性脑卒中患者中,3个月以上随访截尾时间的死亡率和再发血管事件与CRP水平相关,CRP增高组发生率比CRP正常组分别高2.71和1.81倍[OR合并分别为3.71和2.81,95%CI分别为(2.40,5.73)和(1.42,5.53)]均有统计学意义(P＜0.05);CRP测量值与缺血性脑卒中复发和死亡亦相关,在复发患者中,CRP测量值平均高于未复发者为3.52 mg/L[WMD=3.52,95%CI(-0.01,7.05)],具有一定的临床意义;在死亡患者中,CRP测量值平均高于生存者为8.02 mg/L[WMD=8.02,95%CI(5.68,10.37)],具有统计学意义(P＜0.05).结论 发病时CRP的水平可以初步评价急性缺血性脑卒中患者的预后.     

Mutation screening of mismatch repair gene Mlh3 in familial esophageal cancer

AIM: To shed light on the possible role of mismatch repair gene Mlh3 in familial esophageal cancer (FEC).METHODS: A total of 66 members from 10 families suggestive of a genetic predisposition to hereditary esophageal cancer were screened for germline mutations in Mlh3 with denaturing high performance liquid chromatography (DHPLC), a newly developed method of comparative sequencing based on heteroduplex detection. For all samples exhibiting abnormal DHPLC profiles,sequence changes were evaluated by cycle sequencing.For any mutation in family members, we conducted a segregation study to compare its prevalence in sporadic esophageal cancer patients and normal controls.RESULTS: Exons of Mlh3 in all samples were successfully examined. Overall, 4 missense mutations and 3 polymorphisms were identified in 4 families. Mlh3 missense mutations in families 9 and 10 might be pathogenic, but had a reduced penetrance. While in families 1 and 7,there was no sufficient evidence supporting the monogenic explanations of esophageal cancers in families.The mutations were found in 33% of high-risk families and 50% of low-risk families.CONCLUSION: Mlh3 is a high risk gene with a reduced penetrance in some families. However, it acts as a low risk gene for esophageal cancer in most families. Mutations of Mlh3 may work together with other genes in an accumulated manner and result in an increased risk of esophageal tumor. DHPLC is a robust and sensitive technique for screening gene mutations.     

粒细胞集落刺激因子对亚致死剂量照射后小鼠胸腺细胞周期及近期输出功能的影响

本研究探讨粒细胞集落刺激因子(G-CSF)对急性辐射损伤小鼠胸腺细胞的细胞周期、凋亡及近期输出功能的影响.雌性BALB/c小鼠40只,给予6 Gy60C0 γ线1次性全身照射后随机均分为2组.G-CSF组小鼠给予重组人G-CSF 100 μg/(kg·d),皮下注射,连续14天;时照组小鼠给予等体积磷酸盐缓冲液,皮下注射,连续14天.用流式细胞仪检测照后72小时内胸腺细胞的细胞周期及凋亡细胞比例;应用荧光定量PCR方法检测照后30和60天105个胸腺细胞中T细胞受体重排删除环(sjTREC)拷贝数,以判断胸腺细胞输出功能.结果表明,G-CSF在照后6小时即可使胸腺细胞出现G0/G1期阻滞,G-CSF组vs对照组为(82.0±5.0)％ vs (75.9±2.8)％(P＜0.05),S期细胞比例下降,G-CSF组vs对照组为(10.2±4.8)％ vs( 15.7±2.3)％(p＜0.05),但对G2/M期细胞比例影响不大.胸腺细胞受照后6小时即出现明显凋亡,12小时达高峰.G-CSF组在照后6及12小时胸腺细胞凋亡率分别为(11.5±2.4)％和(15.5±3.3)％,而对照组分别为(16.5±2.2)％和(22.6±0.7)％,两时间点统计学差异明显(p＜0.05).荧光定量PCR结果显示,照后30天G-CSF组胸腺细胞sjTREC拷贝数明显高于对照组(p＜0.01),但照后60天两组胸腺细胞sjTREC拷贝数无统计学差异.结论:G-CSF可调节急性辐射损伤后胸腺细胞周期,减少胸腺细胞凋亡,增加胸腺细胞输出功能,促进中枢免疫恢复.     

地方高校医学生物化学双语教学模式探索

为了提高地方高校医学生物化学双语教学的效果,本文对河南科技大学医学院进行生物化学双语教学的一些经验进行了归纳总结,从教学目的、教师素质提高、教学方法和考评机制等方面探讨了地方高校医学生物化学双语教学的模式,将为地方高校开展医学生物化学的双语教学提供一些新的思路和方法。     

糖尿病视网膜病变粘附分子表达与白细胞粘附的干预性研究进展

糖尿病视网膜病变是糖尿病的重要微血管并发症之一,其发病机制复杂,尚未完全阐明。近年来研究发现白细胞和粘附分子在糖尿病性视网膜病变的病理发生过程中起重要作用。本研究就糖尿病时白细胞和粘附分子表达异常、对视网膜的损害及干预性药物治疗等方面进行了综述。