肿瘤坏死因子—α单抗对烫伤大鼠组织脂多糖结合蛋白mRNA表达 …

探讨肿瘤坏死因子－α单抗治疗对烫伤后组织ＴＮＦ－α及脂多糖结合蛋白ｍＲＮＡ表达、不同器官功能改变的影响。方法采用大鼠３５％体表面积Ⅲ度烫伤模型,检测伤后肺,肝,肠肾等组织ＴＮＦ－α及ＬＢＰｍＲＮＡ水平。结果烫伤后肺,肝,肠,肾等组织ＴＮＦ－α及ＬＢＰＭｒｎａ表达前水平均有显著升高,约为正常对照地１．７－２．５倍。     

线粒体比较蛋白质组学在心肌保护领域的研究和展望

背景 线粒体是心肌保护中最为重要的细胞器之一,心肌缺血损伤抑或后处理,必然会影响细胞核DNA及线粒体DNA转录活性,引起相应表达的蛋白质的变化.比较蛋白质组学,为我们提供了在全貌性了解心肌线粒体蛋白质组表达差异的基础上,探索引起能量代谢障碍的上游分子机制或关键调控环节的手段. 目的 探讨线粒体比较蛋白质组学在心肌保护领域的研究和展望. 内容 对缺血/药物后处理及作用机制、后处理心肌保护的线粒体机制、线粒体蛋白组学研究现状及其与心肌保护、研究进展进行综述. 趋向 比较蛋白质组学技术为心肌保护研究提供了新的方法和思路.     

易-和康复技术改善缺血性中风痉挛性偏瘫随机对照研究

目的 观察易-和康复技术改善缺血性中风后痉挛性偏瘫的临床疗效.方法 将171例缺血性中风后痉挛性偏瘫的患者,通过中央随机系统按2:1比例将患者随机分为治疗组114例、对照组57例.其中,治疗组脱落17例,完成病例观察97例,脱落率为14.9％;对照组脱落8例,完成病例观察49例,脱落率为14.0％.治疗组采用易-和康复...     

Unmarried status is a barrier for access to treatment in patients with metastatic renal cell carcinoma

International Urology and Nephrology - We tested the effect of marital status on cytoreductive nephrectomy, metastasectomy, and systemic therapy rates, as well as on cancer-specific mortality (CSM)...     

43例无卡环义齿修复牙列大部分缺损的临床应用

目的:探讨牙列大部分缺损的无卡环义齿修复效果。方法:选择缺失牙较多的牙列缺损患者43例,制作无卡环义齿(根据患者的情况选择做不同材料金属支架),分别于修复后1~3年内复诊,观察义齿固位、美观、咀嚼功能等,评价义齿修复效果。结果:5例患者义齿断裂,总失败率为11.6%,其余良好。结论:选择适应证,牙列大部分缺损患者采用无卡环义齿修复可达到满意的效果。     

Regeneration of immunocompetent B lymphopoiesis from pluripotent stem cells guided by transcription factors

Regeneration of functional B lymphopoiesis from pluripotent stem cells (PSCs) is challenging, and reliable methods have not been developed. Here, we unveiled the guiding role of three essential factors, Lhx2, Hoxa9, and Runx1, the simultaneous expression of which preferentially drives B lineage fate commitment and in vivo B lymphopoiesis using PSCs as a cell source. In the presence of Lhx2, Hoxa9, and Runx1 expression, PSC-derived induced hematopoietic progenitors (iHPCs) immediately gave rise to pro/pre-B cells in recipient bone marrow, which were able to further differentiate into entire B cell lineages, including innate B-1a, B-1b, and marginal zone B cells, as well as adaptive follicular B cells. In particular, the regenerative B cells produced adaptive humoral immune responses, sustained antigen-specific antibody production, and formed immune memory in response to antigen challenges. The regenerative B cells showed natural B cell development patterns of immunoglobulin chain switching and hypermutation via cross-talk with host T follicular helper cells, which eventually formed T cell-dependent humoral responses. This study exhibits de novo evidence that B lymphopoiesis can be regenerated from PSCs via an HSC-independent approach, which provides insights into treating B cell-related deficiencies using PSCs as an unlimited cell resource.     

东菱迪芙治疗糖尿病合并急性脑梗死的疗效观察

目的评价应用东菱迪芙治疗糖尿病合并急性脑梗死的临床疗效及安全性。方法60例糖尿病合并急性脑梗死患者中,35例采用东菱迪芙10BU隔日静滴,共3次为治疗组,另25例为对照组。比较治疗前,治疗后5d、10d2组神经功能缺损评分及临床疗效,并观察治疗前后5d、10d血浆纤维蛋白原(FG)、凝血酶原时间(PT)、凝血酶时间(TT)、红细胞比积(HCT)。结果治疗组病例神经功能缺损评分及临床疗效在治疗前后以及与对照组比较均有显著意义。结论东菱迪芙治疗糖尿病合并急性脑梗死疗效良好。     

Non‐synonymous alterations in AKR7A3 and ABCA6 correlate with bleeding in aged patients treated with rivaroxaban

Rivaroxaban is an oral anticoagulant that inhibits thrombin and blocks coagulation cascade through directly inactivating factors Xa. Despite rivaroxaban is widely used for prevention and treatment of venous thrombosis, and its common adverse reactions have been reported, including abnormal coagulation, mucosal hemorrhage, hematuria, and intracranial hemorrhage. To explore potential drivers of individual differences in adverse reactions induced by rivaroxaban, we performed whole‐exome sequencing and found that AKR7A3 rs1738023/rs1738025 and ABCA6 rs7212506 are susceptible sites for rivaroxaban‐related bleeding in aged patients treated with rivaroxaban. Gene functional annotation and signaling pathway enrichment indicated that homozygous mutations in AKR7A3 and ABCA6 might alter normal rivaroxaban transport and metabolism, and lead to continuous accumulation of activated drugs and toxic substances in vivo. Our results suggested that interindividual differences in bleeding events induced by rivaroxaban may be potentially driven by genetic alterations related to abnormal metabolism and transport of rivaroxaban.

Study Highlights

• WHAT IS THE CURRENT KNOWLEDGE ON THE TOPIC?

Although rivaroxaban has been wildly used for the prevention and treatment of prevent of deep vein thrombosis without requiring routine coagulation monitoring, the adverse events, such as bleeding following rivaroxaban treatment, has not been fully addressed.

• WHAT QUESTION DID THIS STUDY ADDRESS?

The correlation between genetic variations and rivaroxaban treatment‐induced side effects (e.g., bleeding).

• WHAT DOES THIS STUDY ADD TO OUR KNOWLEDGE?

The AKR7A3 rs1738023/rs1738025 and ABCA6 rs7212506 confer susceptibility to adverse reactions caused by rivaroxaban.

• HOW MIGHT THIS CHANGE CLINICAL PHARMACOLOGY AND TRANSLATIONAL SCIENCE?

This study identified AKR7A3 and ABCA6 genes involved in drug metabolism and transport associated with susceptibility to rivaroxaban‐related bleeding events, and provided supporting evidence for the prevention and treatment of anticoagulant‐caused adverse effects.     

皮内痣伴结缔组织增生性结节一例

患者男,28岁,因左侧胸部单发褐色肿物4年、多发小丘疹1年就诊。皮肤科检查：胸部左侧一1.2 cm × 1.1 cm × 1.0 cm褐色类圆形肿物,质韧,其右侧数个直径3 ~ 5 mm褐色丘疹,左侧腋下淋巴结未触及肿大。手术完整切除肿物后行组织病理检查：肿物和小丘疹内均可见真皮浅层痣细胞聚集成巢,考虑皮内痣;最大肿物...     

血清可溶性 CD36、抵抗素、能量平衡相关蛋白联合预测 2型糖尿病合并非酒精性脂肪性肝病的价值探讨

目的探讨血清可溶性 CD36(sCD36)、抵抗素、能量平衡相关蛋白(Adropin)联合对 2型糖尿病(T2DM)合并非酒精性脂肪性肝病( NAFLD)的预测价值。方法前瞻性选取石家庄市第二医院 2018年 5月至 2021年 5月收治的 90例 T2DM合并 NAFLD病人( A组)、 90例单纯 T2DM病人( B组)同期选取 90例体检健康者(对照组)作为研究对象。通过酶联免疫吸附测定检测研究对象血清 sCD36、抵抗素、 Adropin表达水,平,对比分析三组 sCD36、抵抗素、 Adropin表达水平差异;分析 sCD36、抵抗素、 Adropin表达相关性; logistic回归分析 T2DM合并 NAFLD的影响因素;受试者操作特征曲线( ROC曲线)分析 sCD36、抵抗素、 Adropin三者联合对 T2DM合并 NAFLD的预测价值。结果 A组病人血清 sCD36(63.7±15.6)ng/L、抵抗素( 3.15±0.46)μg/L水平均高于 B组［( 43.8±14.2)ng/L、(2.72±0.68)μg/L］和对照组［( 22.9±5.7)ng/L、(2.58±0.39)μg/L］(P<0.05),血清 Adropin水平(66.28±27.62)ng/L均低于 B组( 86.73±25.46)ng/L和对照组( 128.59±45.22)ng/L,差异有统计学意义( P<0.05)。 sCD36与抵抗素表达水平呈正相关(r=0.29,P<0.05)Adropin与 sCD36表达呈负相关(r=0.57,P<0.05)。 logistic回归分析显示, sCD36,抵抗素,总胆固醇( TC)是 T2DM合并 NAFLD影响因素( P<0.05)。 ROC曲线分析显示, sCD36、抵抗素、 Adropin单独及三者联合预测 T2DM合并 NAFLD的曲线下面积( AUC)分别为 0.81、0.74、0.72、0.89,三者联合对 T2DM合并 NAFLD的预测效能显著高于单指标独立预测( P<0.05)。结论 T2DM合并 NAFLD病人血清 sCD36、抵抗素水平升高, Adropin水平降低,三者联合对预测 T2DM合并 NAFLD具有较高效能。