关节镜下半腱肌、股薄肌重建前交叉韧带

膝关节韧带损伤中，前交叉韧带（anterior cruiate ligament，ACL）损伤占据了较大比例，重建ACL对增加膝关节稳定性起到了积极作用。在微创技术日臻完善、成熟的条件下，我们自1999年11月至2003年11月，在关节镜下应用可吸收界面螺钉（Bio—interference Screw）固定半腱肌、股薄肌肌腱（ST—GT）重建ACL损伤22例，取得良好疗效。     

能谱-X线微束分析检测新生大鼠额叶皮质神经干细胞定向分化为神经元样细胞各时段α辅肌动蛋白的表达

BACKGROUND： Alpha-actinin （ a -actinin） plays a key role in neuronal growth cone migration during directional differentiation from neural stem cells （NSCs） to neurons. OBJECTIVE： To detect in situ microdistribution and quantitative expression of a -actinin during directional differentiation of NSCs to neurons in the temporal lobe cerebral cortex of neonatal rats. DESIGN, TIME AND SETTING： Between January 2006 and December 2008, culture and directional differentiation of NSCs were performed at Department of Histology and Embryology, Preclinical Medical College, China Medical University. Immune electron microscopy was performed at Department of Histology and Embryology and Department of Electron Micrology, Preclinical Medical College, China Medical University. Spectrum analysis was performed at Laboratory of Electron Microscopy, Mental Research Institute, Chinese Academy of Sciences. MATERIALS： Basic fibroblast growth factor, epidermal growth factor, brain-derived nerve growth factor, type-1 insulin like growth factor, and a -actinin antibody were provided by Gibco BRL, USA; rabbit-anti-rat nestin monoclonal antibody, rabbit-anti-rat neuron specific enolase polyclonal antibody, and EDAX-9100 energy dispersive X-ray analysis were provided by PHILIPS Company, Netherlands. METHODS： NSCs, following primary and passage culture, were differentiated with serum culture medium （DMEM/F12 ＋ 10% fetal bovine serum ＋ 2 ng/mL brain-derived nerve growth factor ＋ 2 ng/mL type-1 insulin like growth factor）. MAIN OUTCOME MEASURES： Expression of a -actinin in neuron-like cells was quantitatively and qualitatively detected with immunocytochemistry using energy dispersive X-ray analysis. RESULTS： Immunocytochemistry, combined with electron microscopy, indicated that positive α -actinin expression was like a spheroid particle with high electron density. In addition, the expression was gradually concentrated from the nuclear edge to the cytoplasm and expanded into developing neurites, during differentiation of neural stem cells to neurons. Conversely, energy dispersive X-ray analysis indicated that the more mature the neural differentiation was, and the greater the expression of α -actinin. CONCLUSION： The gradual increase of α -actinin expression is related to growth, development, and maturity of differentiated neuron-like cells, in neonatal rat frontal lobe cortex, at different differentiating time points of NSCs to neurons.     

多层螺旋CT脑血管造影容积重建技术对颅内动脉瘤的诊断及临床应用价值

目的：探讨多层螺旋CT血管造影（MSCTA）容积重建（VR）技术对颅内动脉瘤的诊断及价值。方法：对37例临床怀疑颅内动脉瘤的患者进行MSCTA检查，其中6例另行DSA检查。全部患者CT扫描获得原始图像后采用VR技术对图像进行三维重建，10例同时采用最大强度投影（MIP）进行重建。结果：37例患者MSCTA共发现43个动脉瘤，其中多发动脉瘤5例（4例2个，1例3个）。VR图像上所测动脉瘤直径2.5—38mm。6例DSA患者发现6个动脉瘤，与CTA结果基本相符。结论：MSCTAVR技术是一种简便、微创而且相对可靠的诊断颅内动脉瘤的方法，对于指导手术或介入治疗颅内动脉瘤有重要价值。     

沉默长链非编码RNA HULC增加脑胶质瘤对替莫唑胺的敏感性

目的:研究HULC在脑胶质瘤中的表达,探讨HULC对脑胶质瘤对替莫唑胺敏感性的影响.方法:Real-time PCR检测HULC基因在脑胶质瘤组织中的表达.构建HULC基因表达沉默载体sh-HULC并转染U251细胞,Real-time PCR检测转染效果.MTT法检测HULC基因表达沉默对于U251细胞的增殖能力及对替莫唑胺敏感性的影响.结果:HULC基因在脑胶质瘤表达显著上调,HULC的高表达与脑胶质瘤的低分化及高分期相关.HULC基因的表达沉默载体能够显著沉默HULC基因的表达.HULC表达沉默的U251细胞的增殖活力下调明显,对替莫唑胺的敏感性明显增加.结论:长链非编码RNA HULC在脑胶质瘤中高表达,沉默其表达能够抑制脑胶质瘤细胞的增殖活力并提高脑胶质瘤细胞对替莫唑胺敏感性.     

DNA拓扑异构酶-Ⅱα沉默对脑胶质瘤干细胞增殖和凋亡的影响

目的由U87MG细胞分离并鉴定脑胶质瘤干细胞,研究Topo-Ⅱα基因对脑胶质瘤干细胞增殖和凋亡的影响。方法培养脑胶质瘤U87MG细胞,分离和鉴定其中的胶质瘤干细胞。将Topo-Ⅱα基因特异性的siRNA转染胶质瘤干细胞。转染后,Western Blot检测转染后Topo-Ⅱα蛋白的表达,MTT法检测细胞增殖活力,双标流式法检测细胞凋亡率。结果成功分离出U87MG细胞球细胞,并鉴定其具有肿瘤干细胞特性。转染Topo-Ⅱα基因特异性的siRNA后,胶质瘤干细胞中的Topo-Ⅱα蛋白表达明显降低,同时,细胞增殖能力显著降低,凋亡率明显增加。结论由U87MG细胞系中分离出细胞球细胞具备肿瘤干细胞特性,Topo-Ⅱα基因沉默降低胶质瘤干细胞的增殖能力,促进细胞凋亡。     

CaM和Actin在大鼠皮层神经干细胞定向分化的神经元的共存表达

目的研究大鼠皮层神经干细胞定向分化为神经元过程中钙调蛋白(calmodulin,CaM)与肌动蛋白(actin)的时空表达与两者的共存表达。方法采用细胞培养、免疫组化,图像分析术,免疫荧光双标技术检测神经干细胞定向分化为神经元过程中第1、3、5、7天的钙调蛋白与肌动蛋白的时空表达,共焦激光扫描显微镜(CLSM)下观察二者表达共存变化及其相关性。结果在大鼠大脑皮层神经干细胞定向分化的神经元,CaM与Actin的时空表达随着分化时间的增加,表达逐渐增强,随神经元发育逐渐成熟,CaM与Actin的阳性表达也逐渐延伸到突起,分化7天阳性表达最强。CaM的表达与Actin表达基本一致,共存表达胞体和突起。结论在大鼠大脑皮层神经干细胞定向分化的神经元CaM和actin共存表达,随着神经元分化发育成熟表达增强。