布-加氏综合症介入放射学治疗

目的　探讨布 -加氏综合征不同类型的介入放射学治疗效果。方法　 1例患者经下腔静脉造影后采用球囊成形术 ,其余 3例均造影后行球囊成形 支架置入术。结果　 1例膜型梗阻经球囊成形术后治愈 ,2例节段型梗阻患者经球囊成形术后分别置入“Z型支架 ,1例肝静脉型置入wallstent支架治愈。结论　介入放射学在诊治布 -加氏综合征方面是一种独特、有效的方法。     

视场角设置对虚拟环境中目标判断的影响

目的探讨不同视场角参数对虚拟环境中目标判断的影响规律 ,找出最佳视场角参数。方法利用自行研制基于PC平台的虚拟现实 (VR)实验系统 ,选定 45名年龄在 2 3～ 5 8岁被试者并完成大小与角度判断的实验设计。首先进行 30 0人次的预实验 ,确定引起虚拟环境中目标判断差错的视场角及相应观察任务的差别等级 ,然后开始单因素、4水平的完全随机正式实验。结果方差分析表明 ,不同的虚拟环境视场角对目标判断的真实性有显著性影响 ,当视场角为 60°时 ,目标判断的正确率最大。结论利用VR技术进行载人航天器工效设计、操作训练或其它任务的研究时 ,应考虑视场角的作用 ,适合目标大小判断的视场角参数值宜取 60°,以便获得最大的工效     

Poly-L-Lysine玻片在寡核苷酸芯片制备中的改进

目的 为了制得适合固定未修饰寡核苷酸的芯片，提高检测灵敏性，对Patrick Brown实验室的多聚左旋赖氨酸包被玻片的方法进行改进。方法 玻片经清洗后用缩水甘油-丙氧基三甲氧基硅烷进行硅烷化，然后应用Poly-L-Lysine在玻片表面形成聚合物涂层，经次亚苯基二异硫氰酸盐表面活化后可使寡核苷酸共价连接在芯片表面。设计了各种实验考察方法改进前后芯片表面的性能，并将改进后的玻片初步应用于SARS冠状病毒寡核苷酸芯片检测中。结果 方法改进后芯片表面性能优良：固定效率高、点的同一性好、杂交效率和热稳定性好、寡核苷酸结合牢固、芯片可以重复利用。结论 利用共价连接，方法改进后的芯片表面适合固定未修饰的寡核苷酸，解决了寡核苷酸与玻片之间物理结合不稳定、易剥离的缺陷，提高了芯片检测的灵敏性。     

Kinetics and enzyme involvement in the metabolism of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) in microsomes of rat lung and nasal mucosa.

The rat lung and nasal cavity are two target organs for carcinogenesis by 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). In order to characterize further the enzymes involved in the bioactivation of NNK, detailed kinetic and inhibitory studies were conducted with rat lung and nasal mucosa microsomes, and the results were compared with previous studies. The enzymes in rat lung microsomes catalyzed the alpha-hydroxylation, pyridine N-oxidation and carbonyl reduction of NNK. The apparent Km for the formation of the NNK-derived keto aldehyde, NNK-N-oxide, the NNK-derived keto alcohol and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol were 28.8, 10.4, 7.0 and 178.1 microM respectively. In rat nasal microsomes, alpha-hydroxylation was the predominant pathway and the rate was approximately 200 times higher than that in lung microsomes. The apparent Kms for keto aldehyde and keto alcohol formation in rat nasal microsomes were 9.6 and 10.1 microM respectively. The cytochrome P450 inhibitors metyrapone and carbon monoxide markedly inhibited the metabolism of NNK in both rat lung and nasal microsomes. In rat lung microsomes, alpha-naphthoflavone and monospecific antibodies against P450s 1A2, 2A1 and 2B1 inhibited the formation of keto aldehyde by 39, 46, 64 and 23% respectively. In rat nasal microsomes, alpha-naphthoflavone and antibodies against P450s 1A2, 2A1 and 3A inhibited the metabolism of NNK by 80, 35, 20 and 14% respectively. The results indicate that cytochromes P450 play a major role in the metabolic activation of NNK in rat lung and nasal microsomes, and that there are tissue-related differences in NNK metabolism.     

STUDIES ON NUTRITION AND PRECANCEROUS LESIONS OF THE ESOPHAGUS IN THE ADOLESCENTS

This paper reports the prevalence of chronic esophagitis and nutritional status among 538 young persons aged 15 to 26 years from the high risk area for esophageal cancer. Of these subjects, 166 were from households with history of esophageal cancer and 372 were from households without history of esophageal cancer. The Incidences of chronic esophagltis among male and female adolescents were 37. 6% and 36% respectively, which was significantly higher than those in the low risk area (17%). The frequency of chronic esophagltis in the adolescents in the households with history of esophageal cancer was aiso higher than in those In the households without history of esophageal cancer. The deficiencies of vitamins, especially of riboflavin and ascorbate, are prevalent and severe among these adolescents. Ascorbate deficiency Is correlated with the severity of the chronic esophagltis. These results indicate that chronic esophagltis may be involved in the natural history of esophageal carclnogenesis. Nutrient defic     

羊水细胞培养宫内诊断的应用(附120例分析)

报告120例羊膜腔穿刺羊水细胞培养结果:在成功的99例中,发现二例染色体异常,核型为47,xy,+18和46,xx/47,xx,+F,在21例失败中出现一例眼球畸形。培养成功率为82.5%;染色体异常检出率为2.02%。无一例因羊膜腔穿刺而导致流产,但出现一例轻度羊水栓塞合并症。并对羊水细胞培养在产前宫内诊断中应用价值及术后并发症进行了讨论。     

The dynamic distribution of nitric oxide synthase in the small intestine of mice with intestinal radiation sickness

ＴｈｅｄｙｎａｍｉｃｄｉｓｔｒｉｂｕｔｉｏｎｏｆｎｉｔｒｉｃｏｘｉｄｅｓｙｎｔｈａｓｅｉｎｔｈｅｓｍａｌｌｉｎｔｅｓｔｉｎｅｏｆｍｉｃｅｗｉｔｈｉｎｔｅｓｔｉｎａｌｒａｄｉａｔｉｏｎｓｉｃｋｎｅｓｓＷｅｉＬｉｃｈｕｎ（魏丽春）；ＧｕｏＹａｏ（郭鹞）（...     

人腭扁桃体隐窝上皮结构的研究

采用扫描电镜、透射电镜及光镜免疫金银法观察了31例慢性扁桃体炎患者和8例胎儿的扁桃体隐窝上皮结构及其中细胞的分布。电镜观察发现隐窝上皮表面存在三种类型微隐窝开口,其腔内有浸润细胞、异物及细菌。有三种特化上皮细胞(M细胞)覆盖于Ⅲ型微隐窝开口处,其结构与肠道M细胞相似,其数量随扁桃体炎的反复发作而减少。形态表明微隐窝是浸润细胞和外来抗原的出入口。M细胞与抗原的摄取及传递有关。光镜免疫金银法观察证明上皮浸润细胞中多数为OKT_s~+细胞,其中OKT_4~+者又占多数而OKT_s~+细胞较少,这些细胞是隐窝上皮参于免疫应答的结构基础。     

股骨头缺血性坏死的CT诊断

本文报告了19例股骨头缺血性坏死的CT及多平面重建和三维CT成像表现,并探讨其对术前病变评估的价值,在显示Ⅱ期病变时CT明显优于平片,多平面重建有利于发现股骨头塌陷、关节间隙改变和髋臼改变,三维CT可作为一种补充检查手段。     

福州市区蚊虫孳生习性调查分析

目的:探讨福州城区蚊虫孳生习性与分布现状。方法:调查不同环境孳生地种类与分布,捕捞幼虫计数密度（条/勺）,以人工小时诱捕蚊数计算刺叮率（只/h.m）。结果:调查各类水体 33种 16257处,发现阳性水体32种45522处,阳性率27.8％。建筑工地、高层建筑地下层积水池、开放式排水沟和下水道口等环境积水是城区致倦库蚊的主要孳生环境,建筑工地积水池最高幼虫密度1065～2342条/勺,成蚊刺叮率141.5只/h.m,是普通居民区的16.2倍。白纹伊蚊的孳生环境多达28种,主要为建筑工地和高层建筑地下层水泥池、阳台积水和水缸、桶类、轮胎等容器型积水。结论:城市蚊虫孳生环境与50～60年代明显不同,建筑工地、高层建筑地下层、阳台等建筑类积水和花圃蓄水缸成为现代都市蚊虫重要的孳生环境。