Localization of proteins associated with the outer hair cell plasma membrane in the gerbil cochlea

There is substantial evidence that the motility of mammalian outer hair cells is generated close to or within the plasma membrane. Several analogies between the outer hair cell cortical lattice and the membrane-related cytoskeleton of erythrocytes have been noted. In erythrocytes a member of the anion exchanger protein family, AE1, also known as Band 3, is involved in membrane-cytoskeleton linkage via Protein 4.1. In the following paper, the presence of these two proteins in gerbilline outer hair cells is confirmed by western blot. Furthermore, co-localization of these two proteins was detected in the lateral wall of outer hair cells by immunofluorescence and postembedding electron immunohistochemistry. Band 3 is restricted to this region, whereas Protein 4.1 has a somewhat more dispersed distribution.

Thus, the structure of these sensory receptor cells may result from an adaptation of a strategy used by other motile cells. The proteins investigated likely have a support function and may comprise “pillars” seen between the lateral plasma membrane and the cytoskeleton in micrographs of outer hair cells. The possibility that Band 3 comprises “protein particles” seen in the lateral plasma membrane, or maybe directly involved in the voltage-dependent force generation in outer hair cells, is also discussed.     

Scalable synthesis and antibacterial evaluation of 2‐(3‐(N‐(substituted phenyl)sulfamoyl)ureido)benzothiazoles

A new series of 2‐(3‐(N‐(substituted phenyl)sulfamoyl)ureido)benzothiazoles was synthesized via a one‐pot efficient and scalable method, involving the condensation of 2‐aminobenzothiazoles derivatives, substituted anilines, and chlorosulfonyl isocyanate. The products were obtained in good yield with a simple workup, and their structures were confirmed from their spectral analyses. The synthesized compounds were further screened for their antibacterial activity against Gram‐positive and Gram‐negative pathogenic strains. The molecules show promising activity in the MIC value range of 2–0.25 µg/ml against selected bacterial strains, especially against nonfermentative carbapenem‐resistant bacteria (Pseudo VIM‐2 and Acinetobacter baumanni).     

Phytochemical constituents from Salsola tetrandra

The new norisoprenoid 3beta-hydroxy-5alpha,6alpha-epoxy-beta-ionone-2alpha-O-beta-d-glucopyranoside (1) and the long-chain hydroxy fatty acids 9,12,13-trihydroxyoctadeca-10(E),15(Z)-dienoic acid (2) and 9,12,13-trihydroxyoctadeca-10(E)-dienoic acid (3) were isolated from Salsola tetrandra aerial parts, together with 3,4,5-trimethoxyphenyl-beta-d-glucopyranoside (4), 9-hydroxylinaloyl glucoside (5), taxiphyllin (6), trans-N-feruloyltyramine (7), and S-(-)-trans-N-feruloyloctopamine (8). Their structures were elucidated by extensive spectroscopic analysis and chemical methods. Compounds 6 and 8 displayed mild antibacterial activity against Staphylococcus aureus, whereas compound 6 showed the highest activity in the Artemia salina bioassay.     

Predicting morbidity after robotic partial nephrectomy: The effect of tumor,environment, and patient-related factors

Purpose

To investigate the effect of tumor and nontumor related parameters on perioperative outcomes of robotic partial nephrectomy (RPN).

An analysis of the outcomes for patients with failed primary vitrectomy for rhegmatogenous retinal detachment

Purpose

Our aim was to identify the timing of retinal redetachments and the prognosis for affected patients.

Methods

Retrospective review of electronic patient records and casenotes from two centres. Inclusion criteria were failed primary vitrectomy for rhegmatogenous retinal detachment during a 12-year period. Failure was defined as re- or persistent detachment of the retina prior to a further procedure or at final follow-up.

Results

The records of 133 cases of failed primary PPV for RRD were analysed. The mean age at time of primary surgery was 62.9 years, and mean follow-up was over 3 years from the date of primary surgery. In 72.9 % of cases, the redetachment was diagnosed within 2 months of the primary surgery. Eighty-five percent were diagnosed within 3 months, 89.5 % within 4 months, and 97.7 % within 6 months. For patients in whom the primary surgery had failed, the rates of attached retina at final follow-up were 80.5 %, or 68.8 % if PVR B/C were present at the time of primary surgery. The final follow-up success rates (attached retina with no tamponade) were 66.2 % for all patients, and 59.4 % for the subset with PVR B/C at the time of primary surgery respectively. Of the total, 33.8 % had one further vitreoretinal procedure, 30.8 % two more, 25.6 % three more, and 5.3 % had four or more. Visual outcome was better with final success (attached retina and no tamponade, p?p?Conclusions A short duration of follow-up can over-estimate surgeon success rates for retinal detachment repair. For patients in whom primary surgery has failed, the prognosis for ultimate success is markedly lower than for primary surgery, and most patients require two or more further procedures to achieve this.