A PRELIMINARY STUDY OF THE ACTION OF MALE HOR MONE ON HIGH DENSITY LIPOPROTEIN-CHOLESTEROL

Plasma testosterone and estradiol are determined in 72 patients with abnormal high density lipoprotein. cholesterol (HDL-C) and high density lipoprotein cholesterol/total cholesterol (H/T) ratio values, and in 72 randomly chosen males with normal HDLC and H/T values. The results showed that plasma testosterone levels in the groups with abnormal HDL-C and H/T were obviously lower than those in the controls. Statistically significant differences were found in all the abnormal groups in comparison with the controls (P0.20). Testosterone levels lowered further with increasing age in the groups with abnormal HDL-C and H/T., the most obvious drops were in the groups around 56 years of age (P<0.005). The data indicate that male hormone deficiency may reduce HDL-C and H.'T and facilitate the process of atherosclero_is. Therefore, it seems rational to treat such patients with male sex hormone preparations or to use the traditional Chinese medicines which strengthen Yang (maleness) in a new attempt to treat and prevent CHD.     

Adeno-associated virus vector-mediated systemic delivery of IFN-beta combined with low-dose cyclophosphamide affects tumor regression in murine neuroblastoma models.

PURPOSE: Type I IFNs (IFN-alpha/beta) have shown significant antitumor activity in preclinical models but limited efficacy and significant toxicity in clinical trials. We hypothesized that the antitumor activity of type I IFNs could be enhanced by chronic, low-dose systemic delivery and sought to test this in murine neuroblastoma models. EXPERIMENTAL DESIGN: Continuous liver-generated expression of human IFN-beta (hINF-beta) was achieved through a gene therapy-mediated approach using adeno-associated virus vectors encoding hIFN-beta (AAV hINF-beta). Orthotopic localized retroperitoneal and disseminated models of neuroblastoma were established using three different xenografts. Immunohistochemical analysis and ELISA were used to evaluate the antiangiogenic effect of therapy. RESULTS: The development of both localized orthotopic (retroperitoneal) and disseminated neuroblastoma was prevented in all mice expressing hINF-beta. Continued growth of established retroperitoneal tumors, treated with AAV hINF-beta as monotherapy, was significantly restricted, and survival for mice with established, disseminated disease was significantly prolonged following administration of AAV hINF-beta. Analysis of treated tumors revealed a significant antiangiogenic effect. Mean intratumoral vessel density was diminished and expression of the angiogenic factors vascular endothelial growth factor and basic fibroblast growth factor were both decreased. Finally, combination therapy in which AAV hIFN-beta was used together with low-dose cyclophosphamide resulted in regression of both established retroperitoneal and disseminated disease. CONCLUSIONS: AAV-mediated delivery of hIFN-beta when used as monotherapy was able to restrict neuroblastoma growth due in part to inhibition of angiogenesis. When used in combination with conventional chemotherapy, AAV hIFN-beta was able to effect complete tumor regression.     

子宫脱垂病人合并压力性尿失禁的超声研究

目的研究子宫脱垂病人为什么易合并压力性尿失禁(SUI),如何预测无SUI病人在作矫治子宫脱垂手术后,可能发生压力性尿失禁.方法采用经前庭B超观察了子宫脱垂47人,对照组88人的尿道膀胱.47名子宫脱垂病人中,28人合并不同程度的压力性尿失禁(female stress urinary incontinence,SUI).检查指标是压力期尿道膀胱连接位UVJ-M(Mobility of Urethrovesical Junction),静止期近端尿道长度PUL-r(Proximal Urethra Length at rest).结果子宫脱垂妇女的UVJ-M和PUL-r与对照组有极显著差异,P＜0.001.将UVJ-M＞1.0cm,PUL-r≤2.0cm作为诊断SUI的临界值,子宫脱垂妇女发生SUI的可能性高达85.1%(40/47)其中显性的SUI占59.6%,潜在性的占25.5%.在子宫脱垂无SUI表现的19人中,潜在性的SUI占63.2%(12/19).结论用经前庭B超检查尿道膀胱是术前评估子宫脱垂妇女是否需作尿失禁矫治术最理想的方法,并有利于选择手术方式.     

四种口服头孢菌素的体外抗菌活性的比较研究

本文报道口服Cefixime对900株需氧菌和厌氧菌的体外抗菌作用,并与其它口服的头孢克罗、头孢羟氨苄和头孢氨苄进行了比较。Cefixime对链球菌具较强作用,但对葡萄球菌的作用较差;对肠球菌无效。对肠杆菌科细菌的抗菌活性四者之中以Cefixime最强。Cefixime对头孢氨苄耐药株仍具高度活性,其MIC均值可降低60～1200倍以上。绿脓杆菌、硝酸盐阴性杆菌、鲁氏不动杆菌和产碱杆菌对Cefixime和其它3种头孢菌素的敏感性均较差。四者对厌氧菌的作用除厌氧球菌外其抗菌活性都很弱。Cefixime对β-内酰胺酶稳定,口服吸收良好,抗菌作用强,是一个值得进行临床研究的口服头孢菌素。     

持续压力超负荷对兔左心室细胞凋亡的影响

OBJECTIVE: To study the effect of chronic pressure overload on the apoptosis of the left ventricle myocytes in rabbits. METHODS: Rabbit models of chronic pressure overload-induced heart failure were prepared in which dynamic changes of apoptotic myocytes in the left ventricle were observed by way of terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay. RESULTS: Only a few apoptotic cells was observed in the sham-operated group, while in the experimental group, the apoptotic left ventricle myocytes significantly increased after operation, presenting a peak level between day 3 and day 7. Seven days after the operation, the apoptotic myocytes began to decrease and till day 14, the apoptotic cell number had been smaller than that measured on day 1. When signs of heart failure set in, the apoptotic myocytes were again increased (P<0.001). CONCLUSION: During chronic pressure overload, myocyte apoptosis in the left ventricle is elevated at the early stages and undulates subsequently, with the peak occurring before hypertrophy is obvious.     

小水囊在小月份中期妊娠引产中的应用价值

目的探讨两种引产方法在小月份中期妊娠中的应用。方法40例年龄18．35岁，停经12．17wk，要求引产的病例随机分为两组各20例，分别用米非司酮配伍米索前列醇引产，米非司酮配伍米索加用小水囊联合引产终止妊娠。结果两种引产方法对比分析，从引产至临产时间、总产程、出血量及失败率、住院时间比较，差异有显著性，从清宫率和感染率方面比较，两组无显著差异。结论双米加用小水囊应用于小月份中孕引产是一种高效、安全、经济的引产手段，值得临床推广。     

The Receptor Role of Glycophorin A during the Invasion of Erythrocytes by Plasmodium Falciparum

Under the condition of in vitro culture,the effects of glycophorin A(GPA)of human erythrocyte membrane,anti-GPA IgG,α_1-acid glycoprotein,ovomucoid,wheat germ agglutinin,etc.on the invasion of erythrocytes by P.falciparum were observed in this study.The results show that the combination ofGPA with P.falciparum merozoites has high specificity,high affinity and atendency to saturation,and produces specific biological effects.These confirm forthe first time from the characteristics of the ligand-receptor interaction that GPAis a receptor recognized and bound by P.falciparum merozoites.