细胞因子Irisin缓解高糖诱导骨髓间充质干细胞凋亡的机制研究

背景 高糖可诱导骨髓间充质干细胞(bone-marrow mesenchymal stem cells,BMSCs)凋亡,而细胞因子Irisin对糖毒性所致的细胞损伤具有保护作用.目的 探讨细胞因子Irisin对高糖环境中BMSCs凋亡的影响及其作用机制.方法 复苏BMSCs并将其分为对照组(Vehicle)、高糖诱导组(high glucose,HG)、Irisin干预组(HG+irisin;Irisin)和抑制剂组(HG+irisin+compound C,CC).各组细胞培养3 d后,流式细胞术检测细胞凋亡率.Western blotting检测凋亡相关蛋白Bcl-2/Bax比值和磷酸化AMP依赖的蛋白激酶(phosphorylated AMP-activated protein kinase,p-AMPK)、AMPK的表达水平.结果 与Vehicle组相比,HG组BMSCs细胞凋亡率显著升高(P<0.05);Irisin可降低高糖诱导的BMSCs细胞凋亡(P<0.05),提高Bcl-2/Bax的蛋白表达比(P<0.05),同时激活AMPK.当特异性地抑制AMPK后,Irisin缓解高糖诱导BM-SCs凋亡的作用明显减弱.结论 Irisin可通过激活AMPK缓解高糖诱导的BMSCs凋亡.     

A movable microelectrode array for chronic basal ganglia single-unit electrocorticogram co-recording in freely behaving rats

The basal ganglia–cortical circuits are important for information process to brain function. However, chronic recording of single-unit activities in the basal ganglia nucleus has not yet been well established. We present a movable bundled microwire array for chronic subthalamic nucleus (STN) single-unit electrocorticogram co-recording. The electrode assembly contains a screw-advanced microdrive and a microwire array. The array consists of a steel guide tube, five recording wires and one referenced wire which form the shape of a guiding hand, and one screw electrode for cortico-recording. The electrode can acquire stable cortex oscillation-driven STN firing units in rats under different behaving conditions for 8 weeks. We achieved satisfying signal-to-noise ratio, portions of cells retaining viability, and spike waveform similarities across the recording sections. Using this method, we investigated neural correlations of the basal ganglia–cortical circuits in different behaving conditions. This method will become a powerful tool for multi-region recording to study normal statements or movement disorders.     

Development of a Valid Simplified Chinese Version of the Oxford Hip Score in Patients With Hip Osteoarthritis

Background

Although the Oxford Hip Score has been translated and validated in several languages, there is currently no Chinese version of the outcomes measurement. Our study aims to crossculturally adapt and validate the Oxford Hip Score into a simplified Chinese version.

Questions/purposes

We tested the (1) reliability; (2) validity; and (3) responsiveness of the Chinese version of the Oxford Hip Score.

Methods

First we translated the Oxford Hip Score into simplified Chinese, then back into English, then held a consensus meeting to achieve the final simplified Chinese version. Then we evaluated the psychometric properties of Chinese version of the Oxford Hip Score in patients undergoing total hip arthroplasty (THA). All patients undergoing THA between July and December 2012 were invited to participate in this study; a total of 108 (79% of 136 invited) did so. To assess the test-retest validity, all participants completed the Chinese version of the Oxford Hip Score again with a 2-week interval. Pearson correlation coefficient was used to evaluate the construct validity between the Chinese version of the Oxford Hip Score and visual analog scale (VAS), Harris hip score, and eight individual domains of the SF-36. Responsiveness was demonstrated by comparing the pre- and postoperative scores of the Chinese version of the Oxford Hip Score.

Results

The test-retest reliability with intraclass correlation coefficient (0.937) and internal consistency with Cronbach’s alpha (0.91) were excellent. The Chinese version of the Oxford Hip Score correlated with the Harris hip score (0.89, p < 0.01), VAS (−0.79, p < 0.01), and Physical Functioning (0.79, p < 0.01) and Bodily Pain (0.70, p < 0.01) domains of SF-36, which suggested construct validity. No floor or ceiling effects were found. The effect size and standardized response mean values were 3.52 and 3.31, respectively, indicating good responsiveness.

Conclusions

The Chinese version of the Oxford Hip Score showed good reliability, validity, and responsiveness in evaluating standard Chinese-speaking patients with hip osteoarthritis undergoing THA. It can be used by clinical surgeons as a complement to the traditional outcome measures.     

大剂量云南白药治疗应激性胃溃疡的疗效观察及护理

目的观察大剂量云南白药治疗应激性胃溃疡的疗效及总结护理对策。方法将2000年9月～2001年12月收治的104例各种原因引起的应激性消化道出血病人作为对照组,选择2002年1月～2003年2月的92例各种原因引起的应激性消化道出血病人作为实验组。两组在常规止血药治疗基础下,实验组加用冰盐水、云南白药局部治疗,对照组加用冰盐水、去甲肾上腺素及凝血酶,观察两组治疗效果。结果实验组:显效62例,有效24例,无效6例,总有效率93.5%;对照组:显效24例,有效31例,无效49例,总有效率52.9%。两组疗效比较,字2=10.61,P<0.01,差异有统计学意义。结论云南白药直接作用于出血部位,能使血小板黏附、聚集,加速凝血酶生成等,从而缩短了凝血过程,保护胃黏膜促进溃疡愈合。     

Expression Characteristics of KAI1 and Vascular Endothelial Growth Factor and Their Diagnostic Value for Hepatocellular Carcinoma

Background/Aims

We tried to investigate the expression characteristics of KAI1, a suppressor of wide-spectrum tumor metastasis, and vascular endothelial growth factor (VEGF), the most common angiogenesis factor, and then to analyze their diagnostic value for hepatocellular carcinoma (HCC).

Methods

The protein and mRNA expression levels of KAI1 or VEGF in HCC tissues and in self-controlled para-carcinoma tissues were analyzed by Western blot and real-time polymerase chain reaction, respectively. Serum levels of KAI1 and VEGF in the patients with HCC, benign liver disease or in healthy controls were quantitatively detected by enzyme-linked immunosorbent assay.

Results

The expression level of KAI1 was downregulated, while the expression level of VEGF was upregulated in the tissues or serum of the patients with HCC. The expression level of serum KAI1 in HCC patients was correlated with TNM staging, intrahepatic metastasis, lymph node or peritoneal metastasis, and portal vein thrombus. In addition to the factors that were correlated with KAI1 expression, VEGF expression was also closely related to the α-fetoprotein level of the patients. The area under the receiver operating characteristic curve for the diagnosis of HCC was 0.907 for KAI1 and 0.779 for VEGF. The sensitivity of serum KAI1 levels in the diagnosis of HCC was 86.96%; the accuracy was 83.06%, while the sensitivity, the accuracy and the negative predictive value were improved to 91.86%, 84.68%, and 78.79% according to the combined detection of KAI1 and VEGF, respectively.

Conclusions

A combined detection of KAI1 and VEGF may greatly improve the efficiency of diagnosis and form a reliable panel of diagnostic markers for HCC.     

Millard法和Tennison法修复单侧完全性唇裂316例比较研究

目的:通过两种不同的方法修复单侧完全性唇裂,比较其结果并基于不同唇裂形态学变化选择最优手术方法。方法:316例单侧完全性唇裂患者随机分为两组。每一组分别为158例患儿,分别采用Millard法和Tennison法行首次唇裂修复。术前唇鼻软组织测量并记录。通过白唇卷、唇红缘、丘比特弓、唇高、鼻孔对称性、鼻翼穹隆外观以及鼻翼基脚位置术后评估分析。结果:采用Preson-χ2检验分析关联性和线性趋势对两组患者进行比较。结果显示,Millard法在鼻孔对称性和鼻翼形态方面效果显著(P=0.004,P=0.002),而Tennison法则在恢复唇高方面效果较为突出(P=0.003)。一种特殊的方法能很好的适用于某些唇裂解剖类型的观点在统计上不被证实。结论:某些术前唇裂解剖特点使得手术者选择一种特殊手术方法,但本研究发现这种特殊的手术方法本质上与其他方法是一样的。单一的手术修复方法并不能达到理想的美学和功能结果,而缝合技术与皮肤切口设计同样重要。     

Treatment of intracerebral glioblastomas with G422 tumour cell vaccine in a mouse model

The aim of this study was to develop a tumour vaccine with the ability to induce and expand higher affinity cytotoxic T lymphocytes and stimulate an effective antitumour immune response. The hypothesis tested was that G422 glioblastoma cells modified with B7-1 and interferon (IFN)-gamma genes could serve as a tumour vaccine. It was found that therapeutic subcutaneous immunizations with this tumour vaccine significantly induced a cytotoxic T-cell response and prolonged the survival of female Kuming mice with intracerebral G422 tumour isografts. The data collectively suggested that G422 glioblastoma cells genetically modified with B7-1 and IFN-gamma genes could serve as a tumour vaccine.     

人骨唾液蛋白非融合荧光蛋白载体的构建及在乳腺癌细胞中的表达

背景：人骨唾液蛋白基因在矿化组织以外的易发生骨转移的人乳腺癌中表达。临床观察显示骨转移处的乳腺癌细胞骨唾液蛋白的表达量要高于原发部位的乳腺癌细胞，因此骨唾液蛋白有可能与肿瘤特异性骨转移的关系密切。研究乳腺癌骨转移可为将来临床的预防和治疗提供新的药物靶点。目的：建立骨唾液蛋白的稳定表达乳腺癌细胞系，观察骨唾液蛋白在乳腺癌骨转移的整个过程中的作用。设计：对照实验。单位：华南理工大学生物科学与工程学院，解放军广州军区广州总医院医学实验中心。材料：实验于2003-11／2004-03在解放军广州军区广州总医院医学实验室完成。质粒、菌种和细胞：pIRES2-EGFP载体质粒，E．Coli．Top10、含有人骨唾液蛋白基因全长的克隆载体pB-hBSP和发生特异性骨转移以及脑转移的人乳腺癌细胞株MDA-MB-231BO和MDA-MB-23lBR。方法：将人骨唾液蛋白基因通过聚合酶链式反应的方法从构建好的pB-hBSP载体中亚克隆出来，在其5’和3’端分别引入BglⅡ和PsiⅠ限制性酶切位点，定向克隆至真核表达载体pIRES2-EGFP，构建重组载体pIRES2-hBSP-EGFP。利用脂质体转染的方法将构建好的重组质粒转入特异性脑转移和骨转移的乳腺癌细胞株MDA-MB-231BR和MDA-MB-231BO中。主要观察指标：pIRES2-hBSP-EGFP重组表达载体的构建。重组表达载体pIRES2-hBSP-EGFP转染乳腺癌细胞。结果：①成功构建人骨唾液蛋白和绿色荧光蛋白非融合表达的真核表达载体pIRES2-hBSP-EGFP。②成功转染特异骨转移和脑转移的乳腺癌细胞株，可在荧光显微镜下观察到荧光蛋白标记，人骨唾液蛋白得到相应表达。结论：真核表达载体pIRES2-hBSP-EGFP的构建及转染可为骨唾液蛋白在乳腺癌骨转移中的作用的体内、外研究奠定一定的基础。