乙型肝炎病毒转基因小鼠体内肝靶向反义RNA抗病毒疗效研究

目的：探讨半乳糖修饰的反义RNA真核表达质粒在乙型肝炎病毒转基因小鼠体内的抗病毒作用。方法：以半乳糖多聚赖氨酸（Gal-PLL)作肝靶向载体，将乙型肝炎病毒基因C区的反义RNA真核表达重组质粒（pCEP4-aC）制备为Gal-PLL－pCEP4-aC。将24只血清HBV DNA、HBsAg阳性的小鼠，随机等分为Gal-PLL－pCEP4-aC治疗组、Gal-PLL-pCEP4对照组和生理盐水阴性对照组，于实验第1天尾静脉分别注射Gal-PLL－pCEP4-aC、Gal-PLL－pCEP4（100μg/只）和等体积的生理盐水，观察治疗前后血清HBV DNA以及HBsAg变化。结果：Gal-PLL－pCEP4-aC治疗组21天时血清HBV－DNA转阴率62．5％（5／8），且7，14，21天时血清HBsAg明显降低；而Gal-PLL－pCEP4组血清HBV DNA转阴1只（1／8），生理盐水组8只均未转阴，两组用药后血清中HBsAg与用药前比较差异性均不明显（P＞0．05）。结论：肝靶向反义RNA能在乙肝基因小鼠体内抑制HBV的复制和抗原表达。     

Experimental study on the bone cement-precoated implant in arthroplasty

Canine hip joints, in vitro and in vivo, were used for arthroplasty: one side was replaced by a bone cement-precoated femoral head prosthesis and the other side by a non-cement-precoated one. Results showed that the interfacial shearing strength between both the implant-cement and the cement-bone interfaces of the cement-precoated ones was obviously stronger than that of the non-cement-precoated ones. Thus, the stability of the prosthesis was remarkably increased in the former than the later.     

铜（Ⅱ）的－N（2－羟基乙基）水杨醛亚胺配合物的合成及抑菌活性

合成了４种－Ｎ（２－羟基乙基）水杨醛亚胺合铜（Ⅱ）类配合物，其结构经红外光谱、元素分析，原子吸收证实，并对其抑菌活性进行了试验。结果表明，配合物Ⅰ、Ⅱ对４种革兰氏阳性菌和一种革兰氏阴性菌有抑菌活性。     

肠外营养与生长激素联合应用对外科大手术术后患者蛋白代谢的影响

目的观察肠外营养与生长激素联合应用对大手术术后患者蛋白代谢的影响。方法将拟实施外科大手术(四类以上手术)熏APACHE-Ⅱ评分>7分的患者30例,入院后随机分为3组熏A组穴n=10雪押肠外营养治疗与生长激素穴金磊赛增雪联合治疗组(PN+GH)鸦B组穴n=10雪押肠外营养治疗组(PN)鸦C组穴n=10雪押正常补液组。其中A组在术后第2天开始给予生长激素皮下肌注,连续7d。结果术后8d内,B、C组患者均处于负氮平衡状态,血清白蛋白与胰岛素样生长因子-Ⅰ(IGF-Ⅰ)维持较低水平,A组在术后5d机体开始恢复氮平衡,血清白蛋白与IGF-Ⅰ水平逐渐上升,A组与B、C组比较差异有显著性(P<0.05)。结论较大的外科手术打击后,单纯给予常规的肠外营养要素不能被机体有效的利用,GH能够提高机体对肠外营养要素的利用率,促进机体蛋白合成。     

带血管蒂岛状筋膜瓣移植治疗晚期类风湿性髋关节炎

目的：阐明带血管蒂岛状筋膜瓣移植治疗晚期类风湿性髋关节炎的疗效。材料和方法：对４２例４８髋晚期类风湿性髋关节炎病人采用以旋股外侧血管降支为蒂的股前外侧筋膜瓣移植行患髋成形术。结果：术后随访４～１０．５年，平均４．５年，髋关节活动范围术前平均１６．５°，术后２２８．６°，按照Ｃｈａｒｎｌｅｙ和吴之康人工髋关节置换术后的疗效评定标准，优良２９髋、很好１３髋、好６髋，尚可、差、很差无。结论：患者年龄在１５～４０岁之间为合适的治疗年龄。与人工髋关节置换术相比，本方法对髋关节正常结构破坏小；一旦手术失败仍可选用其它治疗方法弥补；且并发症少、费用低、远期效果好。     

Tongqiao Huoxue Tang and Buyang Huanwu Tang for Treatment of Vascular Dementia -A report of 36 Cases

To study the effects of Tongqiao Huoxue Tang (通窍活血汤) and Buyang Huanwu Tang (补阳还五汤) onvascular dementia (VaD), 36 VaD patients in thetreatment group were treated with the two decoctions,which were compared with 28 cases in a controlgroup treated with Duxil.     

New Exploration of Needling Manipulations at Point Tianzong

In present clinical practice, point Tianzong (SI 11) is often used to treat pain and numbness in the scapular region and the upper limbs. The authors have tried various needling manipulations at this point and obtained extraordinarily good therapeutic result, as is reported in the following.     

荧光定量聚合酶链反应检测乙肝病毒及其临床意义

OBJECTIVE: To study the relationship between the serum contents of HBV DNA copies and the HBV serum index of immunology and the degree of hepatocyte injury in patients with HBV hepatitis. METHODS: The contents of HBV DNA copies were detected by fluorescence quantitative PCR (FQ-PCR), and the HBV serum index of immunology by ELISA. RESULTS: The contents of HBV DNA copies had a significant difference between HBeAg (+)/anti-HBe (-) group (42 patients) and HBeAg (-)/anti-HBe (+) group (57 patients) or HBeAg (-)/anti-HBe (-) group(11 patients) (P < 0.05), while there was no significant difference between HBeAg (-)/anti-HBe (+) group and HBeAg (-)/anti-HBe (-) group(P > 0.05). The contents of HBV DNA copies had no correlation with the severe degree of hepatocyte injury(P > 0.05). CONCLUSION: Anti-HBe is not the terminal sign of HBV replication. The severe degree of hepatocyte injury has not a direct correlation with the contents of HBV DNA copies.     

土地供给市场化是实现土地资源向土地资本转变的关键

由于传统计划经济形成的政府替代市场的土地审批定价模式存在，我国土地供给的市场机制并没有真正建立，市场在国家宏观调控下对土地资源配置起基础性作用的问题并没有解决，由此已经产生很大的诸如国有资产流失等经济外部性问题。目前改革的重点、难点应集中在审批定价和招标拍卖并存的双轨制方面。当前，为促进土地资源向土地资本转变，政府土地管理体制的改革首当其冲。要以土地供给市场化为核心来设计改革的突破口。