Effects of Tung Oil Composite Regenerating Agent on Rheological Properties and Microstructures of Reclaimed Asphalt Binder

The single light oil regenerating agent has certain limitations on the performance recovery of aged asphalt. In this study, tung oil, dioctyl phthalate (DOP), C9 petroleum resin, and organic montmorillonite (OMMT) were used to prepare the composite regenerating agent, and its optimal mix proportion was determined by the orthogonal experimental design. The rheological properties and anti-aging performance of reclaimed asphalt were studied by the dynamic shear rheometer (DSR) and bending beam rheometer (BBR); and the Fourier transform infrared (FTIR) spectrometer, gel permeation chromatography (GPC), and scanning electron microscope (SEM) were adopted to explore its microstructure, morphology, and mechanism of action. The results show that with the addition of tung oil composite regenerating agent, the rheological properties of aged asphalt can be effectively recovered, even better than that of base asphalt. By using the complex modulus aging index (CMAI) and phase angle aging index (PMAI) it is found that the anti-aging performance of reclaimed asphalt is better than that of base asphalt. With the optimal content of the tung oil composite regenerating agent, the contents of characteristic functional groups and macromolecular asphaltenes in the aged asphalt can be reduced, indicating that the composite regenerating agent is beneficial to the dispersion and dissolution of polar substances in the aged asphalt. After aging, a large number of wrinkles appear on the surface of the asphalt. However, the addition of the tung oil composite regenerating agent can make the asphalt surface smooth, which indicates that the tung oil composite regenerating agent can restore the microstructure and morphology of aged asphalt to a certain extent.     

Integrated gene expression profiling analysis reveals SERPINA3, FCN3, FREM1, MNS1 as candidate biomarkers in heart failure and their correlation with immune infiltration

BackgroundThe purpose of this study was to identify possible diagnostic indicators for heart failure (HF) and to investigate the function of immune cell infiltration in this pathophysiology.MethodsHF datasets from the Gene Expression Metascape database were utilized. R software was used to the identify differentially-expressed genes (DEGs) and perform functional correlation analysis. Least absolute shrinkage and selection operator (LASSO) and Boruta algorithms elimination algorithms were then employed to screen and validate the HF diagnostic variables. Finally, Single-sample Gene Set Enrichment Analysis (ssGSEA) was utilized to assess immune cell infiltration in HF tissues, and the Spearman association between gene expression and immune cell concentration was investigated.ResultsA total of 239 DEGs were screened in this study. SERPINA3 (area under the curve, AUC =0.958), FCN3 (AUC =0.972), FREM1 (AUC =0.954), and MNS1 (AUC =0.948) were identified as diagnostic factors of HF. The gene set differentiation analysis (GSVA) (R package “GSVA”) results showed that the high expression of FREM1 and MNS1 genes was involved in bile acid, fatty acid, and heme metabolism, suggesting that the core gene affects the progression of HF by regulating metabolism. Meanwhile, the high expression of FCN3 and SERPINA3 was related to xenobiotic metabolism, inflammatory response, and adipogenesis.ConclusionsGiven the importance of immune cell infiltration in the genesis and progression of HF, SERPINA3, FCN3, FREM1, and MNS1 may be used as diagnostic variables for HF.     

A PCR-basedRapidNeutralizationAssay for GII.4 NorovirusInfection in HIEC6CellCulture

Because of limited viral replication and lack of cytopathic effect in cell culture,a new PCR-based rapid seroneutralization assay for detection of GII.4norovirus neutralized antibodies was developed with serum samples from acute-phase patients,convalescent-phase patients and healthy controls.According to this study,neutralizing antibodies were detected in 100% of     

MED28在口腔鳞状细胞癌及癌旁组织中的表达及临床特征分析

目的 探讨中介体复合物亚基28蛋白(mediator complex subunit 28 protein,MED28)于口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)、癌旁组织中表达及其与病理特征、预后的关系.方法 选取2015年1月至2020年6月首次在我院口腔颌面外科住院手术的OSCC患者标本142例,其中91份OSCC标本包含癌旁正常组织.免疫组织化学法于OSCC及癌旁组织石蜡切片检测MED28的表达.分析OSCC组织与癌旁组织的MED28表达差异.计算MED28表达结果即切片最终评分的平均值,并以此为标准将OSCC患者分为MED28低表达组和MED28高表达组,用Logistic回归模型分析MED28表达与病理特征的关系,Kaplan-Meier法分析MED28的表达与预后的关系,并用COX回归模型分析预后的影响因素.结果 与癌旁组织比较,MED28在OSCC组织中高表达(P＜0.01).Logistic回归模型分析显示MED28表达与吸烟、淋巴结转移相关(P＜0.05),Kaplan-Meier生存分析显示MED28低表达组总体生存时间(overall survival,OS)显著高于高表达组(P＜0.01).吸烟和性别也是影响预后的相关因素(P＜0.05).结论 MED28在OSCC患者癌组织中高表达,可能与OSCC的发生、发展相关,并影响预后,其表达与吸烟、淋巴结转移有关.     

线粒体去乙酰化蛋白SIRT3在噪声诱导隐匿性听力损失中的作用

目的 探讨线粒体去乙酰化蛋白(sirtuin-3,SIRT3)在噪声性隐性听力损失(noise-induced hidden hearing loss, NIHHL)中的作用。方法 将注射过SIRT3抑制剂[3-(1H-1,2,3-triazol-4-yl) pyridine,3-TYP]与未经处理的C57BL/6J小鼠分别于100 dB白噪声下暴露2 h,并在噪声暴露 (noise exposure, NE) 后1 d和14 d进行测听。利用免疫荧光染色观察内毛细胞 (inner hair cell, IHCs)带状突触(ribbon synapses, RS)的变化。冰冻切片下,4-羟基壬烯醛 (4-hydroxynonenal, 4-HNE)染色观察耳蜗内活性氧(reactive oxygen species, ROS) 的累积。结果 与对照组相比,噪声组及3-TYP组小鼠均在噪声后出现短暂性听力阈值的升高,ROS的累积及带状突触的丢失。与噪声组小鼠相比,3-TYP组小鼠在噪声后第14天听力未恢复正常,带状突触丢失明显,且伴有ROS的显著累积。结论 NE诱发了内耳的氧化应激反应,SIRT3可能通过抵抗氧化应激作用来保护耳蜗带状突触及听功能。     

Effects of Online and Face-to-Face Intuitive Eating Interventions on Body Image and Eating Behaviors among Women in China: A Feasibility Study

Body dissatisfaction and eating disorders have become major global concerns, including in Asian populations. Few studies have examined intervention effects on body dissatisfaction and disordered eating in China, especially for interventions with positive psychological perspectives (e.g., intuitive eating). In this pilot study, 66 women participated in an eight-module intuitive eating intervention delivered online (n = 42; mean age, 30.74 years) and face-to-face (n = 24; mean age, 19.46 years) for 8 weeks. Measures of body image and eating behaviors were used to assess the intervention’s feasibility, acceptability, and initial efficacy. Linear mixed models were used to analyze the data. The intervention had significant effects on both groups, promoting positive body image and intuitive eating and reducing negative body image and disordered eating behaviors. The effects of the online and face-to-face interventions did not differ significantly. Thus, whether delivered online or face-to-face, an intuitive eating intervention may effectively improve Chinese women’s body image and eating behaviors. However, the efficacy of the intuitive intervention in the Chinese context should be confirmed in future studies with designs in randomized control trials.     

钼和硒对心肌细胞存活的协同作用

本文利用体外培养心肌细胞方法,通过直接观察钼和硒对心肌细胞生长发育及自发性收缩的影响,证明了钼和硒同时作用比钼和硒单独作用有更加明显之效果,即证明在这方面钼和硒具有协同作用。     

胸腰椎骨折分型、损伤评分、后凸畸形及神经功能损伤的关联研究

目的 探究腰椎骨折损伤程度与骨折的分型、损伤评分、后凸畸形和神经功能损伤的关联.方法 将患者根据后方韧带复合体(PLC)损伤程度分为PLC完整组和PLC损伤组.通过评判两组患者的骨折分型、损伤评分、后凸畸形及神经功能损伤情况,研究他们与PLC损伤的关系.结果 PLC损伤租的载荷分享评分系统(LCS)评分(7.1士0.8)分和严重程度评分(TLICS) (8.2±0.6)分均比PLC完整组的LCS评分(5.7士0.5)分和TLICS(4.6±0.7)分高.PLC损伤组患者的Denis分型和AO分型亦更严重.PLC损伤组的Cobb角为29°,PLC完整组的Cobb角为19°.PLC损伤组的神经功能不全比例89％,PLC完整组的这一比例为60％.结论 胸腰椎骨折与后方韧带复合体关系密切.PLC损伤程度与骨折分型、损伤评分、后凸畸形和神经损伤程度呈正相关的关系.     

硬膜外预置管用于经产妇分娩镇痛临床研究

目的 探讨硬膜外预置管用于经产妇分娩镇痛临床研究价值.方法 选择足月待产经产妇270例作为研究对象,依据患者需求和分娩镇痛方式将产妇分为3组,A组选择自愿要求分娩镇痛无禁忌证的足月经产妇90例,B组同期硬膜外无禁忌证的足月经产妇90例,另设同期未行分娩镇痛的足月经产妇90例作为C组,记录3组产妇产程时间,观察3组产妇缩宫素的使用率、分娩结局、新生儿窒息率以及产后出血率.结果 产程3组比较差异无统计学意义;会阴裂伤率A、B组比较差异无统计学意义,两组与C组比较差异有统计学意义(P<0.05);A、B组在缩宫素使用率和剖宫产率方面比较差异无统计学意义,两组与C组比较差异有统计学意义(P<0.05).3组产妇在新生儿窒息率、产后出血率等方面比较差异无统计学意义.结论 硬膜外预置管用于经产妇分娩镇痛安全性高,且满足产妇不同阶段镇痛需求,保证镇痛的及时性、有效性,具有重要的临床价值.     

肠内营养与激素治疗对儿童克罗恩病的疗效对比研究

目的比较肠内营养（EN）与激素对儿童活动期克罗恩病（CD）的临床指标和肠黏膜愈合方面的治疗效果,探讨EN治疗儿童CD的应用价值。方法将本院2012年7月-2014年1月确诊为活动期CD的患儿,依据治疗方式的不同将患儿随机分为肠内营养治疗组（EN组）和激素治疗组（激素组）,观察对比CD患儿在0、2、4、6、8、10、12周的各项临床检测指标变化,并通过内镜及组织学检查观察对比CD患儿在0、12周时的肠黏膜炎症改变。结果（1）共48例CD患儿入组,EN组25例,激素组23例,两组患儿的一般资料比较,差异无统计学意义（P〉0．05）,具有可比性;（2）治疗结束（12周）时,EN组的临床缓解率为80％（20／25）,激素组为82．6％（19／23）,两组比较差异无统计学意义（P〉0．05）;（3）治疗后EN组的内镜下缓解率为72％（18／25）,显著高于激素组的34．8％（8／23）（P〈0．05）;EN组的组织学缓解率为60％（15／25）,也显著高于激索组的26．1％（6／23）（P〈0．01）。结论EN是治疗儿童活动期CD的一种有效方法,在诱导黏膜愈合方面较激素治疗更为有效。