收费全文 | 1835篇 |
免费 | 108篇 |
国内免费 | 10篇 |
耳鼻咽喉 | 36篇 |
儿科学 | 145篇 |
妇产科学 | 60篇 |
基础医学 | 219篇 |
口腔科学 | 121篇 |
临床医学 | 139篇 |
内科学 | 394篇 |
皮肤病学 | 70篇 |
神经病学 | 100篇 |
特种医学 | 73篇 |
外科学 | 202篇 |
综合类 | 20篇 |
一般理论 | 1篇 |
预防医学 | 69篇 |
眼科学 | 97篇 |
药学 | 120篇 |
中国医学 | 7篇 |
肿瘤学 | 80篇 |
2024年 | 6篇 |
2023年 | 28篇 |
2022年 | 45篇 |
2021年 | 84篇 |
2020年 | 65篇 |
2019年 | 74篇 |
2018年 | 58篇 |
2017年 | 70篇 |
2016年 | 93篇 |
2015年 | 90篇 |
2014年 | 106篇 |
2013年 | 126篇 |
2012年 | 155篇 |
2011年 | 150篇 |
2010年 | 93篇 |
2009年 | 73篇 |
2008年 | 91篇 |
2007年 | 101篇 |
2006年 | 120篇 |
2005年 | 112篇 |
2004年 | 87篇 |
2003年 | 72篇 |
2002年 | 33篇 |
2001年 | 2篇 |
2000年 | 1篇 |
1999年 | 2篇 |
1998年 | 7篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1995年 | 2篇 |
1994年 | 2篇 |
1992年 | 1篇 |
1985年 | 1篇 |
The co-stimulatory molecules CD80 and CD86 are upregulated on activated antigen-presenting cells (APC). We investigated whether local APC activation, induced by subcutaneous (s.c.) inoculation of lipopolysaccharides (LPS), can be imaged by positron emission tomography (PET) with CD80/CD86-targeting 64Cu-labelled abatacept.
ProceduresMice were inoculated s.c. with extracellular-matrix gel containing either LPS or vehicle (PBS). Immune cell populations were analysed by flow cytometry and marker expression by RT-qPCR. 64Cu-NODAGA-abatacept distribution was analysed using PET/CT and ex vivo biodistribution.
ResultsThe number of CD80+ and CD86+ immune cells at the LPS inoculation site significantly increased a few days after inoculation. CD68 and CD86 expression were higher at the LPS than the PBS inoculation site, and CD80 was only increased at the LPS inoculation site. CTLA-4 was highest 10 days after LPS inoculation, when CD80/CD86 decreased again. A few days after inoculation, 64Cu-NODAGA-abatacept distribution to the inoculation site was significantly higher for LPS than PBS (4.2-fold). Co-administration of unlabelled abatacept or human immunoglobulin reduced tracer uptake. The latter reduced the number of CD86+ immune cells at the LPS inoculation site.
ConclusionsCD80 and CD86 are upregulated in an LPS-induced local inflammation, indicating invasion of activated APCs. 64Cu-NODAGA-abatacept PET allowed following APC activation over time.
相似文献