HBV-DNA阳性育龄妇女HBV血清标志物、前S1抗原的检测分析

目的：探讨HBV—DNA阳性育龄妇女病毒复制与HBV标志物及前S1抗原的关系。方法：对1643例慢性乙型肝炎育龄妇女采用荧光定量PCR法检测血清HBV—DNA,酶联免疫吸附法（ELISA）检测HBV标志物和前S1抗原。结果：HBV—DNA阳性育龄妇女432例。其中,HBsAg阴性者占19．44％,前S1抗原总阳性率55．09％,且随着HBV—DNA载量增加,前S1抗原阳性率也升高。结论：采用HBV—DNA、HBV标志物、前S1抗原联检,才能更准确提供育龄妇女HBV感染诊疗依据,有效控制HBV感染率。     

重点理工科高校大一新生焦虑状况及相关因素的研究

目的探讨重点理工科学校大一新生焦虑状况及相关因素,为进行针对性的干预提供依据。方法以广东某重点理工高校2007级的大一学生为研究对象,采用自行设计一般项目调查表、状态-特质焦虑量表（STAI）、匹兹堡睡眠质量指数量表（PSQI）、应付方式问卷进行测评。结果测查学生不论男女状态焦虑、特质焦虑分均显著高于地方常模;19.29%的学生睡眠质量较差;高状态焦虑组和高特质焦虑组解决问题、求助应付因子分均显著低于低状态焦虑组和低特质焦虑组,而自责、幻想、退避应付因子分则显著高于低状态焦虑组和低特质焦虑组;多元逐步回归分析说明,学生如常采用解决问题应付方式,在一定程度上可避免焦虑的产生,而睡眠质量差、采用自责应对方式则可加重焦虑水平。结论采取有效的认知应对策略其意义是使学生改变应激源,以使其变得威胁较小或变成挑战,把情绪基调训练成兴奋、期待和征服。     

男性CHD患者血清T和LP（a）测定的临床价值

目的：探讨血清睾酮（T）和脂蛋白（a）[Lipoprotein（a）,LP（a）]测定对男性冠心病患者的诊疗价值。方法：对102例疑似冠心病（CHD）的男性患者进行冠状动脉造影检查,根据造影结果分为（CHD）病组和非（CHD）组,分别采用放射免疫分析和免疫透射比浊法进行血清T和LP（a）的测定,并对结果进行分析。结果：男性CHD患者血清T水平明显低于非CHD组（P〈0.01）,而血清LP（a）水平则显著高于非CHD组,两者之间呈负相关。结论：检测血清T和LP（a）对男性CHD患者的辅助诊断、疗效观察和预后判断具有重要价值。     

Adjustable degradation and drug release of a thermosensitive hydrogel based on a pendant cyclic ether modified poly(ε-caprolactone) and poly(ethylene glycol)co-polymer

The convenient and precise fabrication of drug–hydrogel formulations with satisfactory degradability and a well-controlled drug release profile are crucial factors for injectable hydrogel formulations in clinical applications. Here a new injectable thermosensitive hydrogel formed from poly(ε-caprolactone) (PCL)–poly(ethylene glycol)–poly(ε-caprolactone) amphiphilicco-polymers with 1,4,8-trioxa[4.6]spiro-9-undecanone (TOSUO) moieties incorporated in the poly(ε-caprolactone) (PCL)block (PECT) was constructed to provide a route to tailor the degradation and drug release behavior. The effect of hydrophilic cyclic ether moieties on the degradation of and drug release by PECT hydrogels were evaluated in vitro and in vivo. The results indicated that a freeze-dried powder of paclitaxel-loaded PECT nanoparticles rapidly dissolved in water at ambient temperature with slightly shaking and formed a stable injectable in situ drug–hydrogel formulation at body temperature, which is convenient for clinical operations because it avoids the need for pre-quenching or long-term incubation. The paclitaxel distribution was also more quantitative and homogeneous on entrapping paclitaxel in PECT nanoparticles. Further, the small number of pendant cyclic ether groups in PCL could decrease the cystallinity and hydrophobicity and, as a result, the in vitro and in vivo retention time of PECT hydrogels and the release of entrapped paclitaxel could be tuned from a few weeks to months by varying the amount of PTOSUO in the hydrophobic block. Significantly, paclitaxel-loaded PECT nanoparticles and free paclitaxel could be simultaneously released during the in vitro paclitaxel release from PECT hydrogels. A histopathological evaluation indicated that in vivo injected PECT hydrogels produced only a modest inflammatory response. Thus pendant cyclic ether modification of PCL could be an effective way to achieve the desired degradation and drug release profiles of amphiphilicco-polymer thermosensitive hydrogels and PECT hydrogels may be suitable for local drug delivery.     

Salivary and Serum Interleukin-18 in Patients with Oral Lichen Planus: A Study in an Ethnic Chinese Population

The purpose of this study was to investigate the potential pro-inflammatory role of the cytokine interleukin (IL-18) in oral lichen planus (OLP) so as to provide a reliable and early indicator for the diagnosis of OLP. One hundred three ethnic Chinese patients with OLP were enrolled in this study, as were 48 age- and sex-matched controls. IL-18 concentrations in serum and saliva were screened by enzyme-linked immunosorbent assay. The protein content was expressed as picograms per milliliter. OLP patients showed a high-level of IL-18 expression profile in serum compared with the control group (OLP?=?21.32?±?8.26?pg/mL, control?=?12.29?±?5.11?pg/mL, P?<?0.05), and the saliva partner had significantly higher concentrations of IL-18 compared to the control (OLP?=?20.12?±?5.78?pg/mL, control?=?15.60?±?4.17?pg/mL, P?<?0.05). In patients with OLP, serum and salivary IL-18 are elevated, correlating with the severity of illness. These findings may be considered to improve the predictive or prognostic values of inflammatory cytokines for OLP and also to design possible novel therapeutic approaches.     

Endogenous glucocorticoids promote the expansion of myeloid-derived suppressor cells in a murine model of trauma

Stress-dose of glucocorticoid has been demonstrated to be beneficial for trauma patients in clinical studies. Recently, a heterogeneous population of myeloid cells with immunosuppressive activity named myeloid-derived suppressor cells (MDSCs) has been found to accumulate in the trauma host and can be induced by glucocorticoids in vitro. In order to explore the effect of endogenous glucocorticoids on MDSCs under trauma conditions, we blocked the glucocorticoid signal in a murine trauma model using the antagonist of the glucocorticoid receptor RU486 (mifepristone). We found for the first time that RU486 not only blunted MDSC expansion induced by trauma in the spleen, peripheral blood and bone marrow especially at 6 h after traumatic stress but also decreased the survival rate from 100 to 20% in traumatic mice within 7 days. Moreover, neither MDSCs producing arginase-1 nor the morphological characterization of trauma-induced MDSCs was affected by the blockage of the glucocorticoid receptor. Our results suggest that endogenous glucocorticoids may promote MDSCs expansion in a murine trauma model and MDSCs may be beneficial for the trauma host.     

Trmt112 gene expression in mouse embryonic development

Mouse Trmt112, the homologous gene of yeast Trm112 (tRNA methyltransferase 11-2), was initially cloned from RIKEN with uncertain function. The yeast TRM112 is now known to play important roles in RNA methylation. Here, we studied the expression of Trmt112 by in situ hybridization and quantitative real-time RT-PCR (QRT-PCR). A higher expression level of Trmt112 was observed in the brain and nervous system by whole mount in situ hybridization from embryonic day 10.5 (E10.5) to E11.5. At later developmental stages E13.5 and E16.5, abundant expression was prominently found in various organs and tissues including developing brain, nervous system, thymus, lung, liver, intestine, kidney, and cartilage. Furthermore, Trmt112 was persistently expressed from E9.5 to E18.5 on whole embryos and highly expressed in multiple organs at E12.5, E15.5 and E18.5 by QRT-PCR. These results showed that Trmt112 gene was highly and ubiquitously expressed during mouse embryonic development, implying that it might be involved in the morphogenesis of diverse organs and tissues and numerous physiological functions.     

Cellular solitary fibrous tumor (hemangiopericytoma) with anaplasia at cerebellopontine angle--a case report

Cellular solitary fibrous tumor is currently considered a synonym for hemangiopericytoma, as it became increasingly clear that the morphological and immunohistochemical features that separate these two entities have become tenuous, and evidence for a unifying concept has emerged. Furthermore, as no evidence of pericytic differentiation is given in most cases of hemangiopericytoma, this diagnostic term is waning in popularity. We present here a case of cellular solitary fibrous tumor in a 22-year-old man. Neuroimaging revealed a right cerebellopontine angle tumor. Most of the tumor was cellular although some less cellular areas were seen. Sinusoidally dilated large vessels, including staghorn type, were seen. Nuclear pleomorphism and increased mitotic activity (5 mitosis/10 high power field) were regarded as evidence of anaplasia. Diffuse CD34 immunoreactivity and focal positivity for Factor XIIIa were seen in the tumor, which was negative for EMA and S100. The tumor also displayed rich reticulin network. Solitary fibrous tumor at cerebellopontine angle is rare, and 20 such cases (five reported as hemangiopericytoma) have been reported in the English literature.     

高中生真实性特点研究

目的考察不同地区和不同性别高中生在父母和朋友关系中感受到的真实性的特点。方法采用修订的真实性量表,对广东省619名高一～高三的学生进行调查。结果①高中生在父母关系中的真实性低于在朋友关系中的真实性(F=82.57,P<0.01),且城市学生的差异大于农村学生(F=8.58,P<0.01);②农村学生(F=11.07)、女生(F=12.15)在父母关系中的真实性高于城市学生和男生在该关系中感受到的真实性(P<0.01)。结论高中生在朋友关系中比在父母关系中更能真实地表达自己,城市学生和农村学生、男女学生在父母关系中的真实性表达存在差异。