急性非淋巴细胞白血病中MGMT与p53基因突变的研究

目的 探讨肿瘤抑制基因ｐ５３、ＭＧＭＴ在急性非淋巴细胞白血病（ＡＮＬＬ）中的改变。方法 用聚合酶链反应－单链构象多态性（ＰＣＲ－ＳＳＣＰ）技术对３１例ＡＮＬＬ患者、２２例正常人ｐ５３基因第５、６、７、８外显子和ＭＧＭＴ基因第１、４外显子进行突变分析。结果 ３１例ＡＮＬＬ中５例存在ｐ５３基因突变、４例存在ＭＧＭＴ基因突变,其中２例ｐ５３与ＭＧＭＴ同时发生突变。结论 ｐ５３基因突变在白血病发生中起一定     

统计软件平台结构研究

讨论了统计软件平台的概念,抽象描述了统计软件平台模型和统计软件平台体系结构,着重介绍了数据字典,统计分析和历史数据处理.采用层次支持的开放式软件结构,并综合统计事务中的各种统计需求设计统计软件平台,使实现的系统具有良好的动态可扩充性.中间件技术的应用使该平台能满足各种数据应用的特定需要.应用数据字典作为控制参数也使得该平台能够满足不同用户的统计要求.     

准分子激光原位角膜磨镶术后屈光回退与术中角膜床厚度的关系

目的　探讨准分子激光原位角膜磨镶术（ｅｘｃｉｍｅｒ ｌａｓｅｒ ｉｎｓｉｔｕ ｋｅｒａｔｏｍｉｌｅｕｓｉｓ ＬＡＳＩＫ） 矫治高度近视后屈光回退与术中角膜床厚度的关系。方法　对４７６ 例９３８ 只眼在我院接受Ｌａｓｉｋ 治疗的患者中,发生屈光回退的病例９６ 例,１８９ 只眼与未发生屈光回退的病例３８０ 例７４９ 只眼术中角膜床厚度及术前、术后１ ｗ ｋ 、１ 、３ 、６ ｍ ｏ 屈光状态的资料进行对比分析。结果　有屈光回退眼组比无屈光回退眼组平均角膜床厚度小;屈光回退眼组屈光回退程度（ 术后６ ｍｏ 时） 与角膜床厚度呈负相关系,上述结果经统计学检验均有显著性差异（ Ｐ ＜ ００１） ;有屈光回退眼组术后早期（１ ｗｋ） 屈光状态略成过矫,但其后屈光回退程度在０ ．５ａ 内呈加重趋势。结论　高度近视准分子激光原位角膜磨镶术中的角膜床厚度是影响术后屈光稳定性的重要因素     

复发性单纯疱疹性角膜炎患者血清IL-2及SIL-2R水平的研究

目的研究复发性单纯疱疹性角膜炎患者血清白细胞介素-2（IL－2）及其可溶性白细胞介素-2受体（SIL－2R）水平。方法本文测定了30例复发性单纯疱疹性角膜炎患者（实验组）和25例健康人（对照组）血清白细胞介素－2（IL－2）及其可溶性白细胞介素－2受体（SIL－2R）的水平。结果对照组IL－2的水平为5．41±1．45mg·L－1,实验组IL－2R水平为2．80±1.47mg·L-1,2组比较t＝2．90,P＜0．01,说明复发性单纯疱疹性角膜炎患者血清IL－2水平较对照组明显降低。对照组SIL－2R水平为90．86±17．90mg·L－1,实验组SIL－2R水平为171·46±19.30mg·L－1,2组比较t＝5．17,P＜0．01。复发性单纯疱疹性角膜炎患者血清SlL－2R水平较对照组显著升高。结论复发性单纯疱疹性角膜炎患者体内免疫功能紊乱,血清IL－2活性降低、SlL－2R水平升高可能是其原因之一,这也为免疫治疗提供了一定依据。     

Prevention of experimental autoimmune anterior uveitis by anterior chamber-associated immune deviation

目的实验性自身免疫性前葡萄膜炎（ＥＡＡＵ）是研究人前葡萄膜炎的有用模型。本研究旨在观察前房相关免疫偏离（ＡＣＡＩＤ）能否阻止ＥＡＡＵ发生。方法将溶于磷酸盐缓冲液（ＰＢＳ）的牛黑色素蛋白（ＢＭＰ）注射于大鼠右眼前房;对照组动物仅注射ＰＢＳ。７ｄ后,使用添加完全福氏佐剂（ＣＦＡ）的ＢＭＰ和百日咳毒素免疫所有动物。通过临床观察和组织病理学检测葡萄膜炎的严重程度和发病率。通过由注射ＢＭＰ刺激的足垫水肿反应评估迟发型超敏反应（ＤＴＨ）。结果与对照组相比,预先眼内注射ＢＭＰ的大鼠,其ＥＡＡＵ的严重程度和发病率减低,且ＤＴＨ反应受到显著抑制。结论这些资料提示ＡＣＡＩＤ可以用来阻止ＥＡＡＵ的发生。     

增生性玻璃体视网膜病变玻璃体切割物的细胞凋亡观察

目的观察增生性玻璃体视网膜病变玻璃体切割物的细胞凋亡情况 。方法收集60例不同分级的增生性玻璃体视网膜病变患者的玻璃体切割物,核苷酸末端转移酶介导dUTP缺口翻译法(TdT-mediated dUTP nick end label ling method,TUNEL法)标记凋亡细胞,光镜观察。结果60例患者的玻璃体切割物均有细胞凋亡的特征性改变,随着病变程度的加重,非色素细胞凋亡总数逐渐减少,并出现色素细胞凋亡。结论增生性玻璃体视网膜病变存在不同类型细胞的凋亡,细胞凋亡是调控其病变程度的重要机制之一。(中华眼底病杂志,1999,15:81-83)     

B超在近视眼巩膜后葡萄肿诊断中的应用价值

目的 :探讨B超在近视眼巩膜后葡萄肿中的应用价值。方法 :用B型超声仪检查 >3.0 0D的 38例76眼进展性近视眼的巩膜后葡萄肿的发生情况。结果 :76眼中 5 8眼发生后葡萄肿。其中各级后葡萄肿的平均屈光度及眼轴差异均有非常显著性意义 (P <0 .0 1和P <0 .0 0 1)。 1～ 4级后葡萄肿与 0级葡萄肿的弧形斑发生率比较差异有非常显著性意义 (P <0 .0 1)。结论 :B超是诊断巩膜后葡萄肿最安全、快速、可靠的方法 ,对近视眼的预后判断有着重要作用。近视眼的屈光度和眼轴随巩膜后葡萄肿的加重而增加 ,眼底弧形斑的形成与后葡萄肿的形成有密切关系     

The Binding of Cyclosporin A to Human Plasma: An in Vitro Microdialysis Study

Purpose. The human plasma binding of cyclosporin A was studied in vitro using the technique of microdialysis. The effect of temperature on the overall binding interaction between cyclosporin A and human plasma was also investigated. Methods. Flow-through loop-type microdialysis probes were constructed from fused silica tubing and regenerated cellulose tubing with a MWCO of 13000 daltons. Probes were perfused with phosphate buffer (0.5 µl/min) and the concentration of ³H-cyclosporin A in the well-mixed medium (plasma or buffer) was 1200 ng/ml. Relative recoveries of cyclosporin A from plasma or buffer were determined for each probe by separate experiments to measure the solute gain or loss with reference to the perfusate. Results. Recoveries determined by loss were significantly greater than those determined by gain and in each case temperature dependent, with higher recoveries at higher temperatures. The plasma free fraction of cyclosporin A calculated from the recovery data and the perfusate to plasma concentration ratios was dependent on temperature in a log-linear fashion. Mean ± s.d. plasma free fractions expressed in percent were 33.5 ± 4.6, 17.9 ± 3.6, 6.2 ± 0.8, 3.0 ± 0.6, and 1.5 ± 0.2 at temperatures of 4, 10, 20, 30, and 37°C, respectively. Assuming that the enthalpy of binding is constant over the temperature range studied and pseudo-first order conditions exist, the binding reaction at these temperatures was spontaneous, endothermic (H = 74.0 kJ/mole), and entropically driven (S = 0.274 kJ/mole/deg). Conclusions. These results show that the free fraction of cyclosporin A in human plasma is dependent on temperature with the fraction unbound decreasing with temperature in the range of 4 to 37°C. The thermodynamic parameters for the binding of cyclosporin A to plasma components indicate that the reaction is a spontaneous endothermic reaction that is mainly entropy driven, similar to the partitioning of lipophilic molecules from an aqueous to a hydrophobic phase. Moreover, these results show that microdialysis is a feasible method to determine the binding interactions between plasma and cyclosporin A, which indicates the method may be suitable for other difficult binding studies where the solutes have nonspecific binding to separation devices.     

对99例髋臼骨折内固定治疗的分析

从1972年11月到1994年3月,401例髋臼骨折病人中的99例经手术治疗并获随访,包括30例双柱骨折,13例横骨折伴后壁骨折,9例后壁骨折,9例后柱骨折,9例前往骨折伴前壁骨折,13例T形骨折和16例横骨折.平均随访时间为7年(2～14).外科手术入路的选择,后入路53次,髂腹股沟入路23次,延长的髂股骨入路11次,放射状入路9次和结合性入路6次.复位情况用X线平片来评判,解剖复位的有59例(59.6%),接近解剖复位(<2mm的错位)的有19例(19.2%).总结果用Harris Hip Score来评判,优秀的有53例(53.5%),良好的有25例(25.3%),一般的有14例(14.1%),差的有7例(7%).并发症中,有10例(53%)为感染,7例(37%)为静脉栓塞.这些结果表明,对于错位严重的髋臼骨折,切开复位内固定是一种可以选择的治疗方法.     

Effects of eating an uncooked vegetable diet for 1 week

Biochemical and metabolic indicators were monitored in a group of volunteers suffering from a variety of chronic illnesses participating in a week's course on a special uncooked vegetable diet, known as "living food". Unmatched healthy controls ate the same diet cooked for 2 min in a microwave oven. After 1 week on the regimen, serum protein and urea contents decreased and alanine aminotransferase (ALAT) activity increased in both groups, although all within the normal range. Blood glucose increased in both groups to slightly above normal limits but total serum cholesterol dropped about 1 mmol/l from normal starting levels. Serum tocopherol and retinol increased only in the group eating the uncooked diet. In both groups urinary sodium dropped drastically without a significant change in potassium. Serum and urinary phenol and p-cresol diminished also significantly. It is concluded that this vegetable diet may be of some benefit in the short term but any longer-term use requires evaluation.