地塞米松和组胺对人鼻息肉组织黏蛋白MUC5AC mRNA和蛋白表达的影响

目的:研究地塞米松和组胺对培养的人鼻息肉组织黏蛋白MUC5AC mRNA和蛋白表达的影响。方法:实验分为对照组、组胺刺激组和地塞米松干预组,采用RT-PCR和免疫组织化学技术分别检测鼻息肉组织中MUC5AC mRNA和蛋白的表达。结果:组胺刺激组鼻息肉组织MUC5AC mRNA和蛋白表达分别为0.6389±0.0526和0.1934±0.0137,均较对照组(分别为0.3495±0.0357和0.1172±0.0173)明显增加(均P<0.01),地塞米松抑制后两者均明显降低(分别为0.4988±0.0603和0.1444±0.0075)(均P<0.05)。结论:炎性递质组胺可上调黏蛋白表达;地塞米松可下调黏蛋白表达,这可能是它抑制鼻息肉黏液过度分泌的途径之一。     

中医护理慢性湿疹

慢性湿疹常由于急性或亚慢性湿疹的治疗不当,造成湿疹长期不愈或反复发作。病因复杂、诊断分型多样、治疗棘手。现对近年来中医药对慢性湿疹护理进行研究分析。     

Research on depression in Parkinson disease: A bibliometric and visual analysis of studies published during 2012–2021

Background:The diagnosis and treatment rate of Parkinson disease (PD) with depression has a low diagnostic rate, and there is no consensus on the choice of treatment mode. This study evaluates the global research trends of scientific outputs related to depression in PD from multiple perspectives, using a bibliometric analysis and visualization tool to scientifically analyze the knowledge from the literature.Methods:Literature related to depression in PD published from 2012 to 2021 was included and selected from the Web of Science Core Collection database in October 2021. CiteSpace software was used to visualize and analyze co-occurrence analyses for countries, institutions, authors, and keywords.Results:A total of 4533 articles from the Web of Science database were included. The United States made the largest contribution with the majority of publications (1215; 29.40%). Toronto University was the most productive institution. PD, depression, quality of life, dementia, nonmotor symptom, prevalence, anxiety, Alzheimer disease, symptom, and disorder would be significantly correlated with depression in PD. The current hot spots in this field focus on the following: risk factors for depression in PD, assessment scale of depression in PD, and rehabilitation of depression in PD.Conclusions:This analysis not only reveals the current research trends and hotspots but also provides some instructive suggestions on the development of depression in PD.     

A Strategy Toward Convergent Combination Immunotherapy for SARS

Passive Immunotherapyfor viral infections withimmune humanimmunoglobulin has been usedfor many yearsinthe pro-phylaxis andtreatment of infectious disease such as RSV,CMV,rabies,hepatitis Aand B and others.Recently,ad-vances in antibody engineering have allowedthe rapid isolation and pre-clinical development of human monoclonal anti-bodies(Mab)for the treatment of humaninfectious diseases and other conditions.We have explored the use of humanmonoclonal antibodies against the newly emerged S…     

结直肠癌多学科专业数据库的初步建立

目的利用Microsoft Visual Studio 2005开发结直肠癌临床病例资料数据库,初步探讨其在患者资料的系统保存、分析统计以及随访方面的应用价值。方法收集上海交通大学医学院附属第九人民医院普外科2005—2009年入院的946例结直肠癌患者资料,利用Microsoft Visual Studio 2005数据库软件开发建立"结直肠癌多学科专业数据库",患者的文字信息输入由基础信息(12个字段)、术前资料(7个字段)、外科治疗(14个字段)和术后随访(3个字段)4个页面组成。结果在Microsoft VisualStudio 2005软件平台上成功建立了结直肠癌病例数据库,并实现了对资料的存储、添加、检索及分类等功能。结论该数据库数据录入简单、资料检索方便,对结直肠癌病案资料具有较强的处理能力,基本符合临床与科研的需求。     

米索前列醇用于人工流产术前宫颈扩张给药时间的观察

目的　探讨口服米索前列醇用于人工流产术前宫颈软化扩张适宜的给药时间。方法　采用前瞻性对比研究 ,将 1 97例早孕妇女随机分为米索前列醇 4 0 0 μg术前 1h服、米索前列醇 4 0 0 μg术前 2h服用、米索前列醇 4 0 0 μg术前 1h服用配伍 1 %利多卡因宫颈 3点、 9点各注射 2ml以及不采用任何药物的A、B、C、D四组。观察并比较各组宫颈松弛程度、扩宫难易度、手术效果、疼痛程度、术前术后宫腔长度差、手术时出血量及人流综合征等参数。结果　A、B、C组在术前宫颈松弛度和扩宫难易度方面差异无显著性 (P >0 0 5 )。而A、B、C组与D组比较差异具有极显著性 (P =0 0 0 0 )。手术效果以及疼痛评分比较 :A组与B组比较差异无显著性 (P >0 0 5 ) ,A、B组与C组之间差异有显著性 (P =0 0 0 9、P =0 0 0 8) ;A、B、C组与D组比较差异有极显著性 (P =0 0 0 0 )。宫腔长度差及出血量在A、B、C、D组之间差异均无显著性 (P >0 0 5 )。A、B、C组均无人流综合征发生 ,D组 1例人流综合征。结论　人工流产术前口服米索前列醇 4 0 0 μg可以有效地软化扩张宫颈 ,减少受术者痛苦 ;术前 1h和 2h用药同样可以有效地软化扩张宫颈 ;口服米索前列醇配伍利多卡因宫颈局部麻醉镇痛效果更佳。     

重组人胰高血糖素样肽-1联合二甲双胍治疗2型糖尿病的有效性和安全性评价

目的 评价在口服降糖药二甲双胍的基础上餐前皮下注射重组人胰高血糖素样肽-1(rhGLP-1)治疗2型糖尿病的有效性和安全性。方法 在随机、双盲、安慰剂对照的临床试验中,选取42例二甲双胍单药治疗的2型糖尿病患者,随机分为观察组和对照组,每组21例。在口服原剂量二甲双胍的基础上分别接受rhGLP-1 0.2mg(观察组)或安慰剂(对照组)三餐前皮下注射治疗,随访12周,观察空腹血糖、餐后2h血糖和糖化血红蛋白(HbA1c)等有效性指标及低血糖、不良反应和肝肾功能等安全性指标。结果 观察组治疗后HbA1c、空腹及餐后血糖水平较前明显下降(P<0.05),且较对照组下降更为显著(P<0.05)。两组治疗后体质量指数均显著下降(P<0.05),但组间比较无统计学差异(P>0.05);观察组体质量指数下降程度与基线值呈正相关(P<0.01)。观察组和对照组均无确切证据的低血糖发生,两组间胃肠道不良反应发生率无统计学差异(P>0.05)。结论 在口服二甲双胍的基础上给予rhGLP-1三餐前皮下注射治疗可有效降低2型糖尿病患者的空腹及餐后血糖,而不增加低血糖及其他不良事件的发生率。     

从中医脾瘅角度评价OLETF大鼠发病特点

[目的]观察自发2型糖尿病大鼠(OLETF)发病特点及病变过程,为深入研究脾瘅理论提供新的实验平台。[方法]引进OLETF大鼠及正常组同系非糖尿病对照大鼠(LETO),动态观察体质量、腹腔脂肪含量、脂体比、口服葡萄糖耐量、高胰岛素正葡萄糖钳夹实验葡萄糖输注率及血脂变化情况。[结果]模型组体质量、腹内脂肪含量及脂体显著高于正常组,其口服葡萄糖耐量葡萄糖曲线下面积(AUC)显著高于正常组,模型组大鼠稳态葡萄糖输注率均较正常组下降(P<0.05)。血清甘油三酯、胆固醇、游离脂肪酸水平均较正常组显著增高。[结论]OLETF较好体现了中医脾瘅的发病过程及代谢综合征病变特点。     

“军卫一号”用户管理的安全漏洞与解决模型

本文分析了“军卫一号”内部网用户管理系统在运行过程中所遇到的网络安全问题，提出了一种全新的“程序代理角色”模型。实践证明，该方法行之有效，在一定程度上可以适应医院管理系统的安全策略。     

HOPX is methylated and exerts tumour‐suppressive function through Ras‐induced senescence in human lung cancer

HOPX acts as a tumour suppressor in various cancers. However, the regulation of HOPX in human lung cancer as well as the mechanism underlying its tumour‐suppressive function has not yet been well elucidated. Here we investigated the epigenetic regulation and molecular mechanism by which HOPX exerts growth inhibitory effects. We found that HOPX was down‐regulated in 12 out of 13 lung cancer cell lines and in 69 out of 120 primary lung tumours at mRNA and protein levels. Patients with lung adenocarcinoma (ADC) exhibited significantly more positive staining of HOPX protein compared with lung squamous cell carcinoma (SCC) (p =0.036). Again in ADC, patients with higher HOPX expression had a significantly longer disease‐free survival (p =0.001). Methylation analysis showed that down‐regulation of HOPX was associated with DNA methylation (p =0.011). To analyse the function of HOPX in lung cancer cells, stable transfection with an expression vector of HOPX was performed. It turned out that HOPX inhibited tumour cell proliferation rate, migration, and invasion, and, more interestingly, forced expression of HOPX enhanced cellular senescence via activation of oncogenic Ras and the downstream MAPK pathway, which in turn led to decreased MDM2 and increased p21. On the contrary, knockdown of HOPX by siRNA resulted in reduced Ras activity, inactivation of the MAPK pathway, and decreased p21 levels, accompanied by reduced cellular senescence. Additionally, the HOPX‐induced senescence pathway was also active in human bronchial epithelial cells. Taken together, our data suggest that down‐regulation of HOPX was related to DNA methylation and that HOPX exerts tumour‐suppressive activity by oncogenic Ras‐induced cellular senescence in lung cancer cells. Copyright © 2014 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.