A PCR-basedRapidNeutralizationAssay for GII.4 NorovirusInfection in HIEC6CellCulture

Because of limited viral replication and lack of cytopathic effect in cell culture,a new PCR-based rapid seroneutralization assay for detection of GII.4norovirus neutralized antibodies was developed with serum samples from acute-phase patients,convalescent-phase patients and healthy controls.According to this study,neutralizing antibodies were detected in 100% of     

肝细胞癌低分割螺旋断层放射治疗后非典型放射性肝病的预测因素分析

目的 探讨临床和剂量学参数与肝细胞癌(hepatocellular carcinoma,HCC)低分割螺旋断层放射治疗(helical tomotherapy,HT)后是否发生放射性肝病(radiation-induced liver disease,RILD)的关系.方法 纳入2011年6月至2015年6月于复旦大学...     

白屈菜赤碱对大鼠肺组织的长期毒性作用及其对肺组织中 NF-κB表达的影响

目的：观察白屈菜赤碱对大鼠肺组织形态的长期毒性作用及其对大鼠肺组织中核转录因子κB （NF-κB）表达的影响,探讨其引起大鼠肺损伤的相关机制。方法：将120只Wistar大鼠随机分为对照组（给予生理盐水）和低、中及高剂量白屈菜赤碱组（给予3.7、5.6和8.4 mg·kg^-1白屈菜赤碱）,每组30只。观察各组大鼠一般情况;HE染色观察各组大鼠肺组织形态表现;酶联免疫吸附（ELISA）法检测各组大鼠血清白细胞介素6（IL-6）、白细胞介素8（IL-8）和肿瘤坏死因子α（TNF-α）水平;实时荧光定量PCR法检测各组大鼠肺组织中NF-κB和细胞间黏附分子1（ICAM-1） mRNA表达水平;Western blotting法检测各组大鼠肺组织中NF-κB和ICAM-1蛋白表达水平。结果：高剂量白屈菜赤碱组大鼠累积死亡率最高,其次为中和低剂量白屈菜赤碱组。各剂量白屈菜赤碱组大鼠体质量和摄食量均明显低于对照组（P<0.05）,高剂量白屈菜赤碱组大鼠体质量和摄食量低于低和中剂量白屈菜赤碱组（P<0.05）。白屈菜赤碱能引起大鼠肺充血和血性腹水。HE染色,随着白屈菜赤碱剂量的增加,各剂量白屈莱赤碱组大鼠肺组织损伤加重。与对照组比较,各剂量白屈菜赤碱组大鼠血清IL-6、IL-8和TNF-α水平升高（P<0.05）;与低和中剂量白屈菜赤碱组比较,高剂量白屈菜赤碱组大鼠血清IL-6、IL-8和TNF-α水平升高（P<0.05）。与对照组比较,各剂量白屈菜赤碱组大鼠肺组织中NF-κB和ICAM-1 mRNA和蛋白表达水平升高（P<0.05）,并呈剂量依赖性;与低和中剂量白屈菜赤碱组比较,高剂量白屈菜赤碱组大鼠肺组织中NF-κB和ICAM-1 mRNA和蛋白表达水平升高（P<0.05）。结论：白屈菜赤碱以剂量依赖的方式对大鼠肺组织产生长期毒性作用,中和高剂量白屈菜赤碱可能通过NF-κB活化和产生炎性细胞因子加重中毒性肺损伤。     

人工饲养恒河猴、食蟹猴的繁殖性能初报

目的 探索北京地区人工饲养恒河猴与食蟹猴的繁殖性能,为温带地区猕猴的人工饲养和繁殖方式提供借鉴.方法 对军事医学科学院实验动物中心饲养的317只恒河猴繁殖群(30只雄猴,287只雌猴)和78只食蟹猴繁殖群(8只雄猴,70只雌猴)近两年的繁殖性状进行观察和统计分析.结果 恒河猴母猴妊娠率、繁殖率和成活率分别为60.73%、54.45%和96.89%.食蟹猴母猴妊娠率、繁殖率和成活率分别为79.86%、56.12%和75.00%.结论 食蟹猴和恒河猴可以成功的在温带地区饲养和繁殖,但人工饲养食蟹猴的妊娠率与产仔率较恒河猴高,而仔猴成活率则低于恒河猴.     

帕金森病患者视幻觉发生率及危险因素的调查

目的 研究原发性帕金森病(PD)患者的视幻觉发生率和相关特征,分析潜在的诱发因素.方法 采用自制的调查表调查128例原发性PD患者的视幻觉发生情况;比较有视幻觉与无视幻觉的患者在性别、年龄、病程、简易智能量表(MMSE)评分、Hoehn & Yahr分级、药物使用种类、左旋多巴等效剂量(LDE)等方面的差异;调查有视幻觉的PD患者快速眼动期睡眠行为障碍(RBD)的发生情况.结果 128例患者中,18例(14.06%)发生过视幻觉.其中10例(55.56%)视幻觉的出现频率≥1次/d;11例(61.11%)视幻觉图像为人影;15例(83.33%)伴随RBD.女性患者比例、MMSE评分、Hoehn & Yahr分级、多巴胺激动剂的使用率在有视幻觉和无视幻觉的患者间比较,差异均有统计学意义(P<0.05).结论 视幻觉是PD患者常见的一种非运动性症状,认知功能、病情严重程度、多巴胺激动剂的使用可能与视幻觉的发生有关.     

支持性护理联合心理干预对脑卒中患者负面情绪、防跌倒的影响

目的:探究支持性护理联合心理干预对脑卒中患者负面情绪、防跌倒的影响.方法:选择2017年5月—2019年8月间广州市荔湾中心医院收治的脑卒中患者90例,随机分为对照组和观察组,每组各45例.对照组给予常规治疗和心理护理,观察组给予支持性护理联合心理干预.比较两组干预前后患者负面情绪、生活质量和跌倒情况的差异.结果:干预后,两组患者焦虑自评量表(SAS)评分和抑郁自评量表(SDS)评分均明显下降(P<0.05),其中观察组变化更为显著(P<0.05).干预后,两组卒中专门生存质量表(SS-QOL)均明显上升,其中观察组变化更为显著(P<0.05).干预后,对照组自我效能感量表(GSES)无明显差异(P>0.05),观察组GSES明显上升,两组差异具有统计学意义(P<0.05).观察组跌倒发生率更低(P<0.05).结论:支持性护理联合心理干预能够明显减弱脑卒中患者焦虑和抑郁情绪,提升生活质量和自我效能,同时减少跌倒的发生.     

比格犬ERβ剪切异构体ERβ419沉默表达载体的构建及筛选

目的构建最有效的慢病毒介导RNAi干扰序列,未来将应用于比格犬ERβ419的未知生物学功能的探索。方法模拟比格犬ERβ419靶细胞筛选针对目的基因mRNA设计的最佳干扰序列实验,通过qRT-PCR和Western Blot技术筛选最佳沉默表达载体序列。结果qRT-PCR结果显示,ERβ419-shRNA1(P0.01)和ERβ419-shRNA3(P0.01)的差异表达极显著,Western Blot结果与qRT-PCR结果一致,ERβ419-shRNA3的干扰效果最佳。结论 ERβ419-shRNA3最有效地抑制了目的基因的表达,未来将应用于探索比格犬ERβ419未知生物学功能对比格犬生殖系统影响的研究,并进而预防和治疗比格犬繁殖机能障碍性疾病。     

海口市2010—2020年输入性登革热流行病学特征

Objective This study intends to understand the epidemiological characteristics of imported dengue fever in Haikou city from 2010 to 2020, we provide references for dengue fever prevention and control in Haikou city. Methods The epidemiological investigation and laboratory nucleic acid test data of dengue fever cases in Haikou City from 2010 to 2020 were collected, and analyzed by Excel 2010 and SPSS 20.0 software, to describe the epidemiological characteristics of imported dengue fever cases. Results A total of 72 cases of imported dengue fever were reported in Haikou City from 2010 to 2020, 53 cases were imported from abroad (73.61%) and 19 cases were imported domestically (26.39%), all of which were mild. The number of imported cases from 2017 to 2019 increased by 1 321.68% compared with the average annual imported cases from 2010 to 2016. The cases were concentrated in the main urban area (97.22%), distributed in the streets in the main urban area. The peak of case imported occurred in August-November,in addition, the domestic imported cases were concentrated in September-October. The ratio of male to female was 2.27∶1, and the average age was (34.93 ±1.55) years old. Working, doing business and visiting relatives accounted for 84.22% in China. Overseas workers, business and tourism accounted for 77.78%. There was a significant difference in the reasons for going out between domestic and overseas imported cases (χ2=75.10,P< 0.05). Among the imported sources, Southeast Asia accounted for 94.34% of overseas imported cases, and Guangdong Province accounted for 57.89% of domestic imported cases. The median of onset to diagnosis was 3 days, the first diagnosis rate was 77.78% (56/72), and the screening rate of people who have been infected before entering the country was 28.57%. Most of the dengue viruses infected were type I and type II, accounted for 84.72%. Conclusion The imported cases of dengue fever in Haikou city have gradually changed from scattered occurrence to rapid growth; we should strengthen the publicity and education of dengue fever among migrant workers, businessmen, tourists and relatives in the main urban area of Haikou City, and improve the awareness of disease prevention and medical treatment, strengthen quarantine inspection and medical examination of entry personnel from Southeast Asian countries as well as dengue fever diagnosis and treatment training in grass-roots medical institutions, which can effectively reduce the risk of local transmission caused by imported dengue fever cases. © 2021 Editorial Office of Chinese Journal of Schistosomiasis Control. All right reserved.     

复方七芍降压片对自发性高血压大鼠肾动脉重塑的影响

【目的】探讨复方七芍降压片（三七、白芍、三麻等组成）对自发性高血压大鼠（SHR）肾动脉重塑的干预作用及机制。【方法】将SHR随机分为模型对照组、厄贝沙坦片组、复方七芍降压片组,每组10只,另设10只WKY大鼠为正常对照组。厄贝沙坦片组给予厄贝沙坦片混悬液27 mg·kg-1·d-1灌胃,复方七芍降压片组给予复方七芍降压片混悬液496.8 mg·kg-1·d-1灌胃,正常对照组和模型对照组给予等体积蒸馏水10 m L·kg-1灌胃。每天给药1次,连续给药6周。给药结束后,苏木素-伊红（HE）染色法观察大鼠肾动脉组织病理变化,并测量管壁厚度、中膜厚度及相同位置的管腔直径,计算中膜厚度/管腔直径比值;免疫组织化学法检测肾动脉中Ⅰ型胶原（COⅠ）、Ⅲ型胶原（COⅢ）蛋白表达情况。【结果】与正常对照组比较,模型对照组大鼠肾动脉管壁厚度、中膜厚度/管腔直径比值增高（P<0.01）,肾动脉组织COⅠ、COⅢ表达水平均明显升高（P<0.01）;与模型对照组比较,复方七芍降压片组和厄贝沙坦片组大鼠肾动脉管壁厚度、中膜厚度/管腔直径比值降低（P<0.05）,肾动脉组织COⅠ、COⅢ表达水...     

广州市越秀区食源性非伤寒沙门菌感染的病例对照研究

目的 探索广州市越秀区食源性非伤寒沙门菌感染的流行病学危险因素,为制定相应的预防控制措施提供依据.方法 运用病例对照研究方法,收集2012～2014年广州市越秀区确诊感染食源性非伤寒沙门菌的病例,以1:1比例选择年龄段、性别及所在的居住地匹配者为对照组进行面对面问卷调查,将所得数据用配对t检验或卡方分析,用OR值比较相关危险因素.结果 从调查中发现在发病前5d有将禽蛋和禽肉储于冰箱(P=0.03,OR=2.67),不常洗手(P＜0.01,OR=29.40),不彻底清洗菜板(P＜0.01,OR=25.72)等习惯,在发病前5d曾进食过含禽蛋饭菜(P＜0.01,OR=4.68),是主要的危险因素.结论 食源性非伤寒沙门菌感染的主要危险因素是不良卫生习惯和饮食习惯,改变不良生活习惯,从源头上切断非伤寒沙门菌污染是防控工作的重要方向.