超声介入治疗盆腔囊肿256例处理体会

目的探讨超声下不同方法穿刺介入治疗盆腔囊肿的疗效。方法收集两家医院两年的盆腔囊肿病人256例作为研究对象,采用超声引导下经腹部穿刺介入和经阴道穿刺介入治疗盆腔囊肿,观察其临床疗效。结果6个月后145例单纯性卵巢囊肿、输卵管积水、盆腔包裹积液全部消失,治愈率100%;108例巧克力囊肿治愈率83.3%、显效率10.2%、有效率3.7%、无效率2.78%。结论超声下介入治疗盆腔囊肿,操作简单、创伤小、成功率高、重复性好,深受临床医师和病人欢迎,但要严格掌握好适应证和禁忌证,抽尽囊液,囊壁固化彻底,就会提高成功率、减少复发率,值得推广应用。     

变应性鼻炎基因芯片检测及其基因表达谱的研究

目的 运用基因芯片技术研究变应性鼻炎的基因表达谱。方法 对变应性鼻炎和非变应性鼻炎鼻黏膜组织进行总RNA抽提，纯化后的mRNA进行逆转录制备杂交探针，应用含有12800个人类全长互补DNA（complementary DNA,cDNA)的表达谱芯片对8例变应性鼻炎与非变应性鼻炎鼻黏膜组织进行差异表达谱分析。结果 在变应性鼻炎的基因表达谱中差异表达基因共有734条，上调的基因430条，下调的基因304条，共存性差异表达基因有21条，其中上调基因15条，下调基因6条，在差异表达的基因中，其中有些是炎性反应、免疫应答、免疫调控、神经内分泌网络系统及信号传导的相关基因，这些基因可能与变应性鼻炎发病机制有关。结论 运用基因芯片技术研究变应性鼻炎相关基因，可能为鼻变态反应性疾病的病因和发病机理的研究提供新思路。     

导升明治疗单纯型糖尿病视网膜病变的临床研究

作者用导升明(doxium,calcium dobesilute)治疗单纯型糖尿病视网膜病变患者32例，设对照组26例患者不用导升明，经治疗3月，观察治疗前后的血液流变学和眼底变化。结果表明，治疗组患者的全血粘度、血浆粘度、红细胞电泳时间和纤维蛋白原均比服药前降低(P<0.05)，而对照组无明显变化(P>0.05)。治疗组眼底病变趋于稳定和改善者多于对照组(P<0.05)，提示导升明能降低糖尿病患者的血液粘度，改善和稳定其眼底病变。 （中华眼底病杂志，1995，11：224-226）     

迟发性外伤性硬脑膜外血肿的诊治进展

迟发性外伤性硬脑膜外血肿的预后和能否早期诊断、处理密切相关。本文对其定义、发生率、发生机理、临床特点、早期诊断方法和预后等问题进行了综述。     

Cloning and characterization of a novel splice variant of the brain-specific protein densin-180

Densin-180 is a brain-specific synaptic protein of the o-sialoglycoprotein family. It functions in specific adhesion between presynaptic and postsynaptic membranes at glutamatergic synapses. We have cloned a novel splice variant of densin-180 from a fetal brain cDNA library and termed it LRRC7. It is located on 1p31 and consists of 8 exons. It encodes a putative protein of 216 amino acids which contains a nuclear localization signal and four leucine-rich repeats (LRRs). LRRs are 20-29 residue sequence motifs present in a number of proteins with diverse functions. Expression analysis of LRRC7 shows it is expressed ubiquitously while its splice variant densin-180 is brain-specific.     

Identification and expression analysis of a novel splice variant of human Sprouty1 gene

Sprouty (SPRY) was first identified in a genetic screen in Drosophila to be an antagonist of fibroblast growth factor (FGF) and epidermal growth factor (EGF) signaling, seemingly by inhibiting the Ras/MAP kinase pathway. During large scale DNA sequencing of the human fetal brain cDNA library, we cloned a novel splice variant of human Sprouty1 gene, and termed it human Sprouty1b (SPRY1b). It has the same deduced protein as Sprouty1a, which has been reported. And like other members of Sprouty family, SPRY1b deduced protein also has a C-terminal cysteine-rich region. According to the search against human genome database, SPRY1b was mapped to 4q25-28. Expression analysis of SPRY1a and SPRY1b shows that they are hardly expressed in adult human, but have different expression patterns in fetus, which confirmed that SPRY1 is an important gene during fetal development.     

纵隔神经源性肿瘤临床病理特征和外科治疗

目的　探讨原发性纵隔神经源性肿瘤的临床病理特征和外科治疗原则。方法　总结125 例纵隔神经源性肿瘤的诊治经验。所有病例均经手术和病理证实。结果　神经源性肿瘤占同期原发性纵隔肿瘤及囊肿的22 .0 % (125/568) 。其中神经鞘瘤48 .9 % (61/125) ,神经纤维瘤27 .2 % (34/125) ,节细胞神经瘤13 .6 % (17/125) ,其他少见肿瘤10 .4 % (13/125) 。无临床症状而于查体时发现78例,占62 .4 % 。肿瘤位于后纵隔117 例。手术切除率98 .4 % (123/125) ,术后并发症发生率4 .0 % (5/125) ,手术死亡率0 .8 % 。恶性肿瘤的3 年生存率18 .2 % 。结论　纵隔神经源性肿瘤的临床特征与病理类型相关,无论良恶性均首选手术治疗,不同的病理类型有不同的预后。     

肝癌自发性破裂治疗方式的选择

肝癌自发性破裂,以往多倾向保守治疗,近年也有人提倡手术治疗。我们对本院１９９５年１０月至１９９８年３月收治的１１例１２次肝癌自发性破裂的病例进行回顾性分析,旨在探讨肝癌自发性破裂治疗方式的选择。１临床资料１．１一般资料本组男９例,女２例。１例发生２次...     

糖原诱出小鼠腹腔巨噬细胞的形态学及细胞化学研究

本实验用电镜及定量细胞化学技术证明糖原诱出的小鼠腹腔巨噬细胞(Mφ)体积增大,皱折和伪足增多。细胞质中吞噬体和溶酶体增多。酸性磷酸酶(AcPase)、非特性酯酶(ANAE)、琥珀酸脱氢酶(SDH)活性增强,而5核苷酸酶(5Nase)活性则与对照组无明显差别。