冷冻治疗女阴尖锐湿疣118例经验体会

尖锐湿疣（Condyloma acuminatum，CA）是人乳头瘤病毒（HPV）引起的一种常见的性传播疾病（STD）。近年来cA的发生率迅速增高，在欧美国家成为最常见的性传播疾病，我国近10a来发现CA病例逐年增多，在STD中仅次于淋病。目前治疗CA的方法很多，最常用的有激光、冷冻、免疫、药物、手术等。我院于2001年6月-2004年10月，与郑州大学第三附属医院和周口市中医院合作，用液氮冷冻治疗女阴部尖锐湿疣（均经病检证实）118例，效果满意，现报道如下。     

Combination bcl-2 antisense and radiation therapy for nasopharyngeal cancer.

PURPOSE: A wide variety of tumors depend on the dysregulation of Bcl-2 family proteins for survival. The resulting apoptotic block can often provide a mechanism for resistance to anticancer treatments, such as chemotherapy and radiation. This current study evaluates the efficacy of combining systemically delivered Bcl-2 phosphorothioate antisense (Bcl-2 ASO) and radiation for nasopharyngeal cancer therapy. RESULTS: Antisense uptake was unaffected by 0, 3, or 6 Gy radiation. Radiation decreased the fraction of viable C666-1 cells to 60%, with a further decrease to 40% in combination with Bcl-2 ASO. Despite a modest in vitro effect, Bcl-2 ASO alone caused the regression of established xenograft tumors in mice, extending survival by 15 days in a C666-1 and by 6 days in a C15 model. The survival times for mice treated with both Bcl-2 ASO and radiation increased by 52 days in C666-1 and by 20 days in C15 tumors. This combination resulted in a more-than-additive effect in C666-1 tumors. Less impressive gains observed in C15 tumors might be attributable to higher expression of antiapoptotic Bcl-2 family proteins and limited drug distribution in the tumor. Retreatment of C666-1 tumors with the Bcl-2 ASO-radiation combination, however, was effective, resulting in mice surviving for >80 days relative to untreated controls. CONCLUSIONS: Our results show that the Bcl-2 ASO and radiation combination is a highly potent therapy for nasopharyngeal cancer. Further examination of combination therapy with radiation and other Bcl-2 family-targeted anticancer agents in both preclinical and clinical settings is definitely warranted.     

The Synergistic Inhibitory Effect of STI571 in Combination with Arsenic Trioxide (As2O3) on Multidrug-Resistant Leukemic Cells

OBJECTIVE To study the synergistic effect of STI571, an inhibitor of tyrosine kinase, in combination with arsenic trioxide As2O3 on a multidrug-resistant leukemia cell line expressing bcr-abl.METHODS The cytotoxic effect of STI571 alone or in combination with different concentrations of As2O3 on the bcr-abl and mdr1 -positive leukemia cell line, K562-n/VCR, was examined by the MTT method.RESULTS One μmol/L of STI571 alone had no significant cytotoxic effect on K562-n/VCR cells. However the cytotoxic effect increased markedly when combined with As2O3 at concentrations of 10-5, 10-6, 10-7 and 10-8 mol/L. The IC50 of K562-n/VCR cells in As2O3 group was 1.879 μmol/L, with. Upon addition of STI571, the IC50 decreased to 0.155 μmol/L resulting in a synergistic cytotoxic effect on K562-n/VCR ceils that was increased 12.1 times.CONCLUSION A combination of STI571 with As2O3 has a more powerful inhibitory effect on leukemia cells expressing positive bcr-abl and positive mdrl compared to the effect with As2O3 alone.     

2000年～2004年广东地区医院血液系统药物利用分析

目的:了解广东地区血液系统药物的利用现状及趋势。方法:对2000年～2004年广东地区60多家医院血液系统药物的品种、用药金额等进行统计、分析。结果:血液系统用药金额呈逐年上升趋势;合资及国产药用药金额逐年上升;用药金额最多的为重组人粒细胞集落刺激因子。结论:国内企业应加强研发力度,进一步提高市场占有率,更好地满足广大患者的需要。     

荧光分光光度法测定注射用盐酸拓普替康的含量

目的:建立注射用盐酸拓普替康的荧光光度测定法.方法:以水为溶剂,采用荧光分光光度法测定注射用盐酸拓普替康的含量,测定波长为ex-380.8nm,em=521.6nm.结果:平均回收率99.81%,RSD为0.87%,线性范围为0.16～1.6μg·ml-1.结论:该方法简便,准确,适用于注射用盐酸拓普替康的含量测定.     

当归妇康胶囊剂成型工艺的研究

目的:确定当归妇康胶囊剂成型工艺条件.方法:考察不同辅料对浸膏粉吸湿性的影响;以颗粒的流动性与吸湿性为指标,用单因素法优选制粒工艺条件.结果:最佳制粒工艺条件为:浸膏粉(药材提取物:淀粉=5:2)与微晶纤维素按7:1的比例混匀,以95%乙醇为润湿剂制软材,醇用量为浸膏粉的5%.结论:成品颗粒的流动性好,临界相对湿度约为65%.     

高效液相色谱法测定复方六月雪颗粒中齐墩果酸的含量

目的:建立高效液相色谱法测定复方六月雪颗粒中齐墩果酸含量的方法.方法:色谱柱为symmetry C18柱,流动相为甲醇-乙腈-水-冰醋酸(72:13:15:0.4),检测波长为210 nm.结果:线性范围0.21～6.39 μg,r=0.999 8.平均加样回收率101.5%,RSD=3.2%(n=6).结论:方法简便,灵敏、准确,建立的方法可用于复方六月雪颗粒的质量控制.     

救急行军散中雄黄的含量测定

目的:建立救急行军散中雄黄的含量测定方法.方法:采用分光光度法对散剂中雄黄所含有的砷进行定量分析.结果:砷的线性范围为2.5～22.5μg,r=0.9995,平均回收率为100.1%,RSD为1.0%.结论:方法简便可行、重线性好,适用于该制剂的质量控制.     

醋酸地塞米松片补充检验方法的研究

对现行版<中国药典>"醋酸地塞米松片"质量标准提出改进意见,建立高效液相色谱法测定醋酸地塞米松片中的醋酸地塞米松含量.色谱柱:Diamonsil(TM)钻石C18(4.6mm×200mm,5μm);流动相:甲醇-水(70:30);检测波长:240nm;流速:1.0ml·min-1.醋酸地塞米松的线性范围为11.16～39.06μg·ml-1,r=0.9999,平均回收率为98.82%,RSD为1.76%.本方法简单,专属性强,重现性好,易于操作.     

浅谈医院药房药品分包装管理

药品包装是保证药品质量的重要因素,离开药品包装就谈不上药品质量.现在的药品生产企业越来越重视药品包装,小包装越来越多,但是还有很多药品实行大包装,调剂时需要分包装.为了保证分包装后药品的质量,必须加强管理工作.