全文获取类型
收费全文 | 12729篇 |
免费 | 613篇 |
国内免费 | 88篇 |
专业分类
耳鼻咽喉 | 168篇 |
儿科学 | 263篇 |
妇产科学 | 73篇 |
基础医学 | 1433篇 |
口腔科学 | 281篇 |
临床医学 | 824篇 |
内科学 | 3178篇 |
皮肤病学 | 282篇 |
神经病学 | 1319篇 |
特种医学 | 428篇 |
外科学 | 2125篇 |
综合类 | 64篇 |
预防医学 | 255篇 |
眼科学 | 322篇 |
药学 | 1072篇 |
中国医学 | 77篇 |
肿瘤学 | 1266篇 |
出版年
2023年 | 74篇 |
2022年 | 167篇 |
2021年 | 274篇 |
2020年 | 189篇 |
2019年 | 208篇 |
2018年 | 292篇 |
2017年 | 216篇 |
2016年 | 254篇 |
2015年 | 281篇 |
2014年 | 340篇 |
2013年 | 424篇 |
2012年 | 706篇 |
2011年 | 733篇 |
2010年 | 435篇 |
2009年 | 400篇 |
2008年 | 690篇 |
2007年 | 732篇 |
2006年 | 754篇 |
2005年 | 681篇 |
2004年 | 640篇 |
2003年 | 600篇 |
2002年 | 585篇 |
2001年 | 271篇 |
2000年 | 303篇 |
1999年 | 283篇 |
1998年 | 146篇 |
1997年 | 132篇 |
1996年 | 90篇 |
1995年 | 81篇 |
1994年 | 72篇 |
1993年 | 71篇 |
1992年 | 161篇 |
1991年 | 156篇 |
1990年 | 172篇 |
1989年 | 185篇 |
1988年 | 163篇 |
1987年 | 163篇 |
1986年 | 171篇 |
1985年 | 172篇 |
1984年 | 110篇 |
1983年 | 85篇 |
1982年 | 58篇 |
1981年 | 49篇 |
1980年 | 43篇 |
1979年 | 77篇 |
1978年 | 44篇 |
1977年 | 45篇 |
1972年 | 51篇 |
1971年 | 45篇 |
1969年 | 51篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
31.
32.
Norihiro Ishida Koji Hayashi Asuka Hattori Keiichiro Yogo Toru Kimura Tatsuo Takeya 《Journal of bone and mineral research》2006,21(1):48-57
We found that a chemokine receptor gene, CCR1, acts downstream of NFAT2 in RANKL-stimulated RAW264 and bone marrow cells. The upstream regulatory region of CCR1 showed RANKL-dependent and CsA-suppressible promoter activity. Downregulation of the expression and function of CCR1 suppressed cell migration. INTRODUCTION: We previously reported that the expression of NFAT2 induced by RANKL is a key process for progression to multinucleated cells in an in vitro osteoclastogenesis system. Identifying the target genes of NFAT2 would thus be informative about the differentiation process. We focused here on chemokine and chemokine receptor genes that act downstream of NFAT2 in RAW264 cells as well as osteoclast precursors prepared from bone marrow cells. MATERIALS AND METHODS: RAW264 mouse monocyte/macrophage line cells were cultured with or without cyclosporin A (CsA) in the presence of RANKL or glutathione S-transferase (GST). Osteoclast precursors were prepared from bone marrow cells. RANKL-inducible and CsA-suppressible genes were searched for by microarray analysis, and expression was confirmed by quantitative RT-PCR. Promoter activity was measured by luciferase gene reporter assay. Short interfering (si)RNA for CCR1 was introduced in RAW264 cells. Cell migration activity was examined using a Boyden chamber assay. RESULTS AND CONCLUSIONS: We identified the chemokine receptor gene CCR1 as a gene showing significant differential expression profiles in osteoclastogenesis in the presence versus the absence of CsA, an inhibitor of NFAT. This property was unique to CCR1 among the chemokine and chemokine receptor genes examined in both RAW264 and bone marrow cells. The upstream regulatory region was isolated from CCR1, and its RANKL-dependent and CsA-suppressible promoter activity was confirmed. The functional significance of CCR1 was assessed by monitoring the migration of cells in a transwell migration assay, and this activity was abolished when either CsA- or CCR1 siRNA-treated cells were used. Moreover, treatment with a Galpha inhibitor pertussis toxin (PTX) or methiolynated-regulated on activation, normal T cells expressed and secreted (Met-RANTES), an antagonist of CCR1, suppressed multinucleated cell formation in the bone marrow cell system. Together, these results suggest that the CCR1 signaling cascade is under the control of NFAT2 and seems to enhance the migration of differentiating osteoclasts. 相似文献
33.
Yoko Saeki Adachi Toshiko Itazawa Motokazu Nakabayashi Tatsuya Fuchizawa Yoshie Okabe Yasunori Ito Yuichi Adachi Gyokei Murakami Toshio Miyawaki 《Allergology international》2006,55(2):167-171
BACKGROUND: A new electronic mesh nebulizer, eMotion is known to have higher performance compared to conventional nebulizers. However, there are some concerns about whether too much delivered dose might cause side effects with higher frequency. METHODS: To evaluate the safety and usefulness of the nebulizer, we measured changes in heart rates and lung functions of 73 asthmatic children when they inhaled 1 microg/kg of procaterol with eMotion or a conventional nebulizer, Junior BOY. RESULTS: In 34 children with mild asthma exacerbation, physical findings, lung function and transcutaneous oxygen saturation levels were improved after inhalation using both nebulizers. No adverse effects including significant increase of heart rate were found. Improvements in the rates of the parameters were comparable. When response to beta2-agonist inhalation was checked in 39 children in stable condition, similar degrees of improvement in lung function were observed, and heart rates did not change after inhalation with either nebulizers. CONCLUSIONS: Safety and efficacy was comparable between eMotion and a conventional nebulizer when it was used to administer beta2-agonists in asthmatic children. However, from the fact that eMotion needs only 3-4 minutes to inhale 2 mL solution, eMotion could be more useful for most children who usually do not prefer longer inhalation time with conventional compressor nebulizers. 相似文献
34.
T Matsunaga N Maseki Y Kaneko K Sampi T Tsuchimochi M Sakurai M Hattori S Takayama 《[Rinshō ketsueki] The Japanese journal of clinical hematology》1992,33(6):829-833
The authors report six cases of non-Hodgkin's lymphoma (NHL) (3B-cell type, one T-cell type, one non-T non-B cell type, one unclassified type) occurring subsequently to autoimmune diseases. The patients were females aged 43 to 70 (median 61). Rheumatoid arthritis was most frequent as the preceding autoimmune disease, and the intervals from the onset of an autoimmune disease to that of NHL were 10 to 36 years (median 20). Polyclonal hypergammaglobulinemia was seen in 4 cases, lymphocytopenia in 3 cases, and conversion to negative PPD reaction in 2 cases. Only one patient had been given corticosteroids, and immunosuppressive agents may not contribute much to the development of lymphoma in patients with autoimmune diseases. 相似文献
35.
Ruri Aoki Tetsuya Arinobu Takeshi Kumazawa Hideki Hattori Hiroshi Noguchi 《Forensic Toxicology》2007,25(1):8-15
An automated on-line method for simultaneous analysis of five phenothiazine drugs by high-performance liquid chromatography
(HPLC)/sonic spray ionization mass spectrometry (SSI-MS) has been established, using backflush column switching. A 400-μl
portion of serum sample diluted 81-fold with distilled water was subjected to the on-line system. In the system, an Oasis
HLB cartridge was used as the precolumn for extraction; large molecules such as proteins in serum were discarded by use of
distilled water containing 0.1% formic acid as a mobile phase. After switching a valve, the analytes trapped in the precolumn
were eluted in the backflush mode and separated by a Chromolith Performance RP-18e column, which is composed of C18-bonded monolithic silica. The column effluents were then introduced into the SSI-MS. The present method provided successful
separation and determination of six phenothiazines including an internal standard. Satisfactory linearities, reproducibility,
and sensitivity were obtained at concentration levels that matched the toxic levels of phenothiazines. All drug peaks appeared
within 18 min, and the system could be reequilibrated in only about 8 min for the next run. Because of the simplicity and
rapidness of the method, it is likely to be useful in the fields of emergency medicine and forensic toxicology. 相似文献
36.
H. Hattori E. Nagata Y. Oya T. Takahashi M. Aoki D. Ito N. Suzuki 《European journal of neurology》2007,14(11):1288-1291
Miyoshi myopathy (MM) is an autosomal recessive distal muscular dystrophy characterized by mutations of the dysferlin gene. Although several pairs of homozygous/heterozygous mutations have been reported, few effective treatments of MM are available. We had observed the decreased serum creatine kinase (CK) before and after administration of dantrolene in the elder brother and the increased serum CK before and after discontinuance of the drug on suspicion of drug-induced hepatopathy in the younger sister. We report a novel pair of heterozygous mutations in the 3'-splicing site of exon 26 and the translation site of exon 28 of the dysferlin gene in two siblings, and effective treatment of their MM with dantrolene. 相似文献
37.
Mitsuru Masaki Tadashi Kuroda Naoki Hosen Hisao Hirota Kazuo Terai Yuichi Oshima Yoshikazu Nakaoka Shoko Sugiyama Ryusuke Kimura Satoshi Yoshihara Manabu Kawakami Norishige Iizuka Yasuhiko Tomita Hiroyasu Ogawa Ichiro Kawase Keiko Yamauchi-Takihara 《Journal of the American Society of Echocardiography》2004,17(4):397-398
A 57-year-old man with a history of renal cell carcinoma presented with presyncope. He underwent nephrectomy years earlier followed by HLA-matched allogeneic peripheral-blood stem-cell transplantation. Echocardiographic investigation revealed a solitary right ventricle mass without contiguous vena caval or right atrial involvement. The mass was pathologically confirmed to be metastatic carcinoma in the right ventricular cavity. This case highlights the need to consider an underlying neoplastic syndrome in patients presenting isolated right ventricle mass by echocardiography. 相似文献
38.
Takashi Ishimori Satoru Nakano Yasutane Mori Reiko Seo Taro Togami Tetsuya Masada Toshiaki Kusuhara Motoomi Ohkawa Seigo Nagao Yuichi Yamashita Satoshi Sugiura 《Magnetic resonance in medical sciences》2007,6(4):225-229
PURPOSE: We assessed the feasibility of utilizing three-dimensional (3D) phase sensitive inversion recovery (IR) images for preoperatively determining deep brain stimulator position. METHODS: We measured geometric distortion with a grid phantom and evaluated images of 3 volunteers to determine optimum imaging parameters for 3D phase sensitive IR. RESULTS: Geometric distortion measured less than 1.0%. Respective inversion and recovery times, which provided high T(1) contrast between the subthalamic nucleus and adjacent tissue, were 200 and 4000 ms. In studies of 3 volunteers and 2 patients, the subthalamic nucleus was clearly depicted in 3D phase sensitive IR images. The measured coordinates of the subthalamic nucleus agreed well with those calculated by conventional estimation from midpoint of the anterior and posterior commissure. CONCLUSION: Three-dimensional phase sensitive inversion recovery was useful in visualizing the subthalamic nucleus for effective deep brain stimulation. 相似文献
39.
40.
Tsuneo Namba Hongxi Xu Shigetoshi Kadota Masao Hattori Tooru Takahashi Yasuhiko Kojima 《Phytotherapy research : PTR》1993,7(3):227-230
The inhibitory effects of glycoproteins separated from a hot water extract of corn silk (U-CSE) on the formation of IgE antibodies after primarily and secondarily challenged responses with dinitrophenyl (DNP)-ovalbumin (OVA) antigen in mice were investigated using the passive cutaneous anaphylaxis (PCA) test. When U-CSE was given intranasally or intraperitoneally the day before primary immunization, IgE antibody production was strongly inhibited. Furthermore, it was found that new formation of IgE antibodies was readily inhibited by U-CSE administration in mice with high levels of IgE after primary immunization. It was also found that U-CSE markedly suppressed IgE antibody formation in secondarily challenged responses with the antigen. U-CSE may be clinically applicable to type I allergic diseases. 相似文献