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991.
F. S. Baranova A. A. Berman Yu. M. Zaretskaya 《Bulletin of experimental biology and medicine》1980,89(1):44-46
1-Antitrypsin (1-AT) reduces the intensity of transformation of human peripheral blood lymphocytes stimulated by phytohemagglutinin. The degree of inhibition is determined by the antiprotease activity of the 1-AT. Maximal inhibition of transformation was shown to be 50%. Participation of 1-AT in the control of activity of lymphoid tissue cells is postulated.Laboratory of Immunogenetics, Research Institute of Transplantology and Artificial Organs, Ministry of Health of the USSR, Moscow. (Presented by Academician of the Academy of Medical Sciences of the USSR V. N. Orekhovich.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 89, No. 1, pp. 35–36, January, 1980. 相似文献
992.
Cooperative transformation of murine fibroblast NIH3T3 cells by hepatitis C virus core protein and hepatitis B virus X protein 总被引:7,自引:0,他引:7
Co-infection with hepatitis B virus (HBV) and hepatitis C Virus (HCV) is associated with increased frequency in the development of hepatocellular carcinoma (HCC). Here, we demonstrated that HBV X protein (HBx) and HCV core cooperate to transform mouse fibroblast NIH3T3 cells. They additively stimulated cell growth, especially in the absence of serum growth factors. In addition, co-expression of HBx and HCV core had additive effects on the induction of anchorage-independent cell growth as well as on the secretion of matrix metalloproteases, which may contribute to increased metastatic potential. Furthermore, the cells expressing both viral proteins exhibited higher tumorigenicity, as demonstrated in athymic nude mice. 相似文献
993.
A new haplogroup pattern displayed in Fujian Han in China 总被引:1,自引:0,他引:1
Yu M Zhang Y Xue Y Chen F Wang Q Huang X Wang B Yu Y Liu A Ma L Shi R Lu F Shi Z Zhang Y Cheng W Ai Q Xu F Huang C Chen B Yang H Kang X Sun Y Zhang G Li P Fu S 《Journal of human genetics》2002,47(2):95-98
Human Y-chromosomal binary polymorphisms have been considered to preserve the paternal genetic legacy and provide evidence
on human evolution and the genetic relationships among and demographic history of different populations. To reveal the genetic
origin and immigration of the Fujian Han, 13 binary markers on the Y chromosome were used to screen Fujian Han by allele-specific
polymerase chain reaction. The results indicated that the M9G marker was highly prevalent (96.20%), suggesting a significant genetic drift. In addition, M122C frequency was only 22.78%, and M45A and M103T were default. The distinctive haplogroup frequencies (H1, H5, and H6/7/8) imply that the haplogroup pattern is a relatively ancestral and interim type.
Received: October 13, 2001 / Accepted: December 3, 2001 相似文献
994.
O. G. Andzhaparidze N. M. Chaplygina N. N. Bogomolova V. D. Lotte I. B. Koptyaeva Yu. S. Boriskin 《Archives of virology》1987,96(1-2):17-28
Summary We have shown that a latent infection of herpes simplex virus type 2 (HSV-2) can be established in a human neuroblastoma cell line IMR-32 if the infected cells are cultured at 40°C. In the present study, viral polypeptides and cellular heat-shock proteins which were synthesized in HSV-2 infected IMR-32 cells cultured at 40°C were analyzed by polyacrylamide gel electrophoresis. It was found that the synthesis of late viral polypeptide ICP 5 was markedly reduced in the infected cells at 40°C as compared with those at 37°C. Although infection of IMR-32 cells with HSV-2 at 40°C resulted in shutoff of cellular protein synthesis, it was found that some cellular heat-shock proteins (90, 72 and 70 kd polypeptides) were synthesized and accumulated intracellularly. These findings suggest that modification of cascade regulation of HSV-2 polypeptide synthesis and/or accumulation of heat-shock proteins may be involved in the incomplete arrest of virus growth and in survival of the infected cells, leading to the establishment of HSV-2 latency in IMR-32 cells. 相似文献
995.
996.
O. Ya. Kaufman Yu. L. Perov A. K. Boikov T. B. Timashkevich 《Bulletin of experimental biology and medicine》1977,84(1):1053-1056
In experiments on rats the ultrastructure of the muscle tissue of the inferior vena cava after disturbance of the outflow of blood, of the muscular coat of the stomach after resection of 50% of its fundus, and of the muscular coat of the cecum after constriction of its ascending portion was studied. Activation of smooth muscles was shown to reflect the phase of injury to the ultrastructure of the cells, followed by processes of intracellular regeneration. Analysis of the ratio between DNA-synthesizing and activated cells showed the local origin of the latter from differentiated myocytes.Institute of General Pathology and Pathological Physiology, Academy of Medical Sciences of the USSR. No. 4 Main Board, Ministry of Health of the USSR. Institute of Human Morphology, Academy of Medical Sciences of the USSR. Moscow. (Presented by Academician of the Academy of Medical Sciences of the USSR A. P. Avtsyn.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 84, No. 7, pp. 112–115, July, 1977. 相似文献
997.
M. N. Karpova I. Yu. Abrosimov G. N. Kryzhanovskii 《Bulletin of experimental biology and medicine》1998,126(1):667-669
The pharmacological kindling with corazol was used as a model of chronic cerebral epileptization. In contrast to sham-operated
rats or rats with unilateral occlusion of the common carotid arteries, bilateral occlusion of these vessels moderated acute
seizures provoked by a single administration of the convulsant corazol and abated the enhanced cerebral seizure readiness
induced by its repeated administration.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 126, No. 7, pp. 30–33, July, 1998 相似文献
998.
广东省人群中人嗜T细胞病毒Ⅰ型感染的血清流行病学调查及其与人类疾病的关系 总被引:7,自引:0,他引:7
收集广东省各类人群血清标本2224份,用间接免疫荧光法检测人类嗜T细胞病毒Ⅰ型(HTLV-1)抗体,结果:HTLV-1抗体阳性35份,总阳性率为1.57%;其中健康人群血清1810份,抗体阳性23份,阳性率为127%;献血员血清248份,抗体阳性1份,阳性率040%;白血病患者血清109份,阳性8份,阳性率734%,与健康人群比较,有非常显著性差异(P<0005);神经系统疾病患者血清57份,阳性3份,阳性率526%。 相似文献
999.
F. Z. Meerson V. G. Pinelis V. B. Kosheley L. Yu. Golubeva T. V. Ryasina E. N. Arsen'eva A. L. Krushinskii T. P. Storozhevykh 《Bulletin of experimental biology and medicine》1993,116(6):1465-1467
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 116, N
o
12, pp. 572–574, December, 1993 相似文献
1000.
Comparison of the autoSCAN-W/A rapid bacterial identification system and the Vitek AutoMicrobic system for identification of gram-negative bacilli 总被引:1,自引:12,他引:1 下载免费PDF全文
M A Pfaller D Sahm C O'Hara C Ciaglia M Yu N Yamane G Scharnweber D Rhoden 《Journal of clinical microbiology》1991,29(7):1422-1428
The autoSCAN-W/A (W/A; Baxter MicroScan, West Sacramento, Calif.) with the new fluorometric Rapid Neg Combo 1 (RNC) panel is a fully automated fluorometric system for identification of both enteric and nonenteric gram-negative bacilli within 2 h. We compared the W/A with the Vitek AutoMicrobic System (Vitek AMS; Vitek Systems, Inc., Hazelwood, Mo.) for identification of 383 clinical isolates of gram-negative bacilli. The API 20E (Analytab Products, Plainview, N.Y.) and conventional biochemical testing were used as the reference systems. The W/A correctly identified 336 isolates (87.7%) to the species level and classified an additional 29 isolates (7.6%) as correct with low probability (overall identification = 95.3%); the Vitek AMS correctly identified 355 isolates (92.7%) to the species level and classified an additional 8 isolates (2.1%) as correct with low probability (overall identification = 94.8%). A common set of 134 isolates of gram-negative bacilli was tested in both participating laboratories as a means of assessing interlaboratory agreement with both the W/A and the Vitek AMS. The overall agreements between the two laboratories were 86% with the W/A and 92% with the Vitek AMS. The W/A performed comparably to the Vitek AMS for identification of most gram-negative bacilli, actually exceeding the Vitek AMS for identification of nonenteric bacilli. Rapid time to identification and a high level of automation make the W/A an attractive system for clinical microbiology laboratories. 相似文献